screening of matrix metalloproteinase inhibitors by microanalysis with fluorescence detection

... conducted with the approval of the ethics review board of the Centre Hospitalier Universitaire de Sherbrooke Detection of matrix metalloproteinases by zymography The gelatinase activity of SF was ... hydroxamate-based inhibitors on arthritic synovial fluids (a) fluids Detection of the inhibitory effect of the matrix metalloproteinase (MMP) inhibitors (inhibitor II and inhibitor III) at different doses by ... Implication of matrix metalloproteinases in proteolytic reactivity found in synovial fluids of RA patients To further assess the contribution of MMP-9 and other members of the MMP family in the NPA of...

Ngày tải lên: 09/08/2014, 08:22

... shown with the blue lines Negative control with no reverse transcription of RNA before real-time PCR is shown with a red line Negative control with no cDNA is shown with the black line and by the ... Human Matrix Metalloproteinase Antibody Array (Raybiotech), which can detect seven MMPs and three tissue inhibitors of metalloproteinases (TIMPs) Both membranes were spotted in duplicate with ... studied within the context of RA [10] It has been shown that IL-7 is produced at higher levels by fibroblast-like synoviocytes isolated from patients with RA and that stimulation of these cells with...

Ngày tải lên: 09/08/2014, 10:23

... Control of matrix metalloproteinase catalytic activity Matrix Biol 2007, 26:587-596 Clark IM, Swingler TE, Sampieri CL, Edwards DR: The regulation of matrix metalloproteinases and their inhibitors ... binding of c-Jun to the MMP-1 promoter was not affected by TGF-β (Figure 6a) Of note, under the same culture conditions, TGF-β-induced binding of SP1 to the promoter region of COL1A2 was abrogated by ... binding of AP2, but not of SP1, to the COL1A1 promoter (Figure 2c) This was distinctly different from the observed increase in binding of SP1, but not Page of 14 of AP2, to the promoter region of...

Ngày tải lên: 12/08/2014, 12:20

... legends, with or without 0.1 mm ATP, with or without 10 lm oligomycin, and with or without mm ouabain The control experiment for the oligomycin effect was performed in the presence of 1% ethanol without ... addition of 0.1 lmol [32P]pNPP with or without 0.03 lmol Pi (a reaction mixture of 100 lL), followed for 10 at 37 °C by the method of Inturrisi & Titus [57] and stopped by addition of mL ice-cold ... activation of pNPPase by KCl in the presence of NaCl with or without oligomycin In the absence of oligomycin, increasing the NaCl concentration decreased pNPPase activity (Fig 2A) In the presence of...

Ngày tải lên: 07/03/2014, 16:20

... selective inhibitors Efforts have been put into both high throughput screening [101] and the design of inhibitors targeting exosites without interfering with the catalytic zinc [8] Such inhibitors ... class of selective matrix metalloproteinase inhibitors J Am Chem Soc 129, 10408–10417 103 Lauer-Fields JL, Whitehead JK, Li S, Hammer RP, Brew K & Fields GB (2008) Selective modulation of matrix metalloproteinase ... attenuated by GSH [35] Other studies have reported activation of proMMP-2 by peroxynitrite, although the activation was followed by a cleavage of the enzymes prodomain, resulting in an enzyme with...

Ngày tải lên: 15/03/2014, 00:20

... tissue inhibitor of metalloproteinases (TIMP)-2, but not by serine and cysteine protease inhibitors, or the ethanol vehicle for the 1,10-phenanthroline (Fig 2A), thereby demonstrating metalloproteinase- mediated ... Yamada H, Hayakawa T, Fujikawa K & Okada Y (2000) Matrix metalloproteinases and tissue inhibitors of metalloproteinases in synovial fluids from patients with rheumatoid arthritis or osteoarthritis Ann ... a2) are indicated Cleavage of type I collagen by proMMP-9 combined with APMA was investigated in five separate experiments, with similar results each time (B) The effect of lg monoclonal anti-(human...

Ngày tải lên: 23/03/2014, 09:21

... The source of Ar ions is a commercial ion gun used for sputtering of materials during depth profiling Ar ion beam with the energy of keV at an incidence angle of 45° and a raster of mm × mm was ... surface of the substrate with bacteria of × 1018 m–2 s–1 We used no charge compensation during treatment of bacteria with argon ions 2.3 SEM imaging Scanning electron micrographs of substrates with ... dimensional distributions of bacteria with out overlapping 2.2 Experimental system Samples were treated either by neutral oxygen atoms in an afterglow chamber of oxygen plasma reactor or by positively charged...

Ngày tải lên: 18/05/2014, 20:33

... prevalence of cryptococcal meningitis of 40 out of 149 (26.8%) HIV-infected adults admitted with a chief complaint of either headache or altered mental status [19] Of 113 patients with either ... prevalence of cryptococcal meningitis was 14 of 93 (15.0%) patients with CD4 counts below 100 cells/mm3, six of 90 (6.7%) with a new diagnosis of HIV, and five of 48 (10.4%) who had been on ART for ... for a quarter of the deaths of HIV-infected adult inpatients in sub-Saharan Africa Patients with cryptococcal meningitis presented with typical symptoms, and universal screening with a serum cryptococcal...

Ngày tải lên: 20/06/2014, 08:20

... the prevention of cartilage degradation is the inhibition of MMPs by targeting either the expression of their genes or the synthesis of the proteins Triterpenoids are a novel family of steroid-like ... chondrocytes expressed low levels of MMP-1 and MMP-13 in the absence of IL-1, and increased levels with addition of the cytokine (Figs 2a,b) Pretreatment of cultures with 300 nM CDDO for 24 hours ... simultaneously with the cytokine: we observed a decrease of 67% (P < 0.1) MMP-1 expression was not significantly inhibited by CDDO added simultaneously with IL-1 Effects of CDDO on expression of Bcl-3...

Ngày tải lên: 09/08/2014, 01:23

... inhibitor of versus matrix controls (MMP-9 )with systemic sclerosismetalloproteinase-1 (TIMP-1) in Comparison of serum concentrations ofhealthy metalloproteinase- 9 (MMP-9) and tissue inhibitor of metalloproteinase- 1 ... growth with skin fibrosis trations factor circulating and with concentrations of transforming Correlation of β (TGFβ) matrix metalloproteinase- 9 (MMP-9) concentrations with skin fibrosis and with ... Soloway PD, Wynn TA: Regulation of hepatic fibrosis and extracellular matrix genes by the Th response: new insight into the role of tissue inhibitors of matrix metalloproteinases J Immunol 2001,...

Ngày tải lên: 09/08/2014, 06:22

... release of matrix metalloproteinase- 9 from peripheral blood mononuclear cells without affecting tissue inhibitor of matrix metalloproteinases-1: suppression by TNF alpha blockage and modulation by ... upregulation of MMP-2 by MIF, whereas the α and β isoform-specific PKC inhibitor Gö6976 did not By contrast, none of the other specific signaling inhibitors mentioned above reduced MMP-2 expression of ... expansion of the synovial lining of the joints Angiogenesis requires proteolysis of the extracellular matrix, proliferation, and migration of endothelial cells, in addition to synthesis of new matrix...

Ngày tải lên: 09/08/2014, 08:22

... in the activation of a number of other members of the MMP family in a range of cell types, including articular chondrocytes [19,20,32] The activation of MMP by other members of the MMP family ... histological grade of degeneration Values are mean ± standard error of the mean *P < 0.05 Gene expression of interleukin-1 and tumour necrosis factor-alpha and correlation with matrix metalloproteinase- 10 ... imaging with LBP A number of studies have demonstrated an increase in expression and activity of a range of matrix- degrading enzymes in IVD degeneration, including the matrix metalloproteinase (MMP)...

Ngày tải lên: 09/08/2014, 14:22

... heterogeneous sample of children with the presence of children without disorders and clinical cases of different severity levels As expected, the Brazilian version of K-SADS-PL showed evidence of convergent ... considered non-clinical by the total problem scale, 41.9% of children with no disruptive disorders were considered non-clinical by the externalizing scale, and 22.7% of children with no affective/anxiety ... 89.7% of children with any psychiatric disorder, the externalizing scale identified 94.3% of children with any disruptive disorder, and the internalizing scale identified 85.3% of children with...

Ngày tải lên: 11/08/2014, 16:22

... TE, Bresnihan B: Early joint erosions and serum levels of matrix metalloproteinase 1, matrix metalloproteinase 3, and tissue inhibitor of metalloproteinases in rheumatoid arthritis Arthritis Rheum ... Relationships of matrix metalloproteinases and their inhibitors to cartilage proteoglycan and collagen turnover and inflammation as revealed by analyses of synovial fluids from patients with rheumatoid ... 17:127-136 36 Joronen K, Kahari VM, Vuorio E: Temporospatial expression of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases in mouse antigen-induced arthritis Histochem Cell...

Ngày tải lên: 12/08/2014, 12:20

... inhibitor of metalloproteinases, matrix metalloproteinases, interleukin-, and tumor necrosis factor-related genes are listed IL, interleukin; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; ... proteoglycan Tissue inhibitor of metalloproteinases Matrix metalloproteinases Interleukin related Tumor necrosis factor related A total of 15,779 rat genes consistent with the quality criteria, ... Rat gene profile of rat nucleus pulposus cells in mono-culture and in co-culture with human MSCs (d) Number of altered rat genes seven days after coculture of rat nucleus pulposus cells with human...

Ngày tải lên: 12/08/2014, 15:22

... secreted by ASMC and stimulation by ILActivity of MMP-12 secreted by ASMC and stimulation by IL1β (A) Gelatin zymography detection of activity of MMPs released into the conditioned media by ASMC ... 48 hours with IL-1β and/or TNF-α in the absence or presence of the specific inhibitors The results of MMP-12 secretion analysed by Western blot was consistent with the data obtained by gelatin ... the induction of a number of inflammatory mediators by ASMC [27,28] Increased expression of IL-1β has been detected in airway epithelial cells and alveolar macrophages of patients with asthma [29,30]...

Ngày tải lên: 12/08/2014, 18:20

... screening (SBVS) and ligand-based virtual screening (LBVS) (10) SBVS consists of the virtual docking of candidate ligands into a protein target followed by the estimation of the probability of ... ligand based virtual screening Structure-based virtual screening (SBVS) starts with a 3-D structure of a target protein and a database of the 3-D structures of ligands as the screening pool It is ... column Screening task Compounds screened No of compounds 536 536 2.5M 46 703 98,435 Protease inhibitors 102,514 102,514 Thrombin inhibitors 25 98,435 COX inhibitors 2.5M 25,300 COX2 inhibitors No of...

Ngày tải lên: 09/09/2015, 18:53

... treated 14 Drawbacks of studies: • First: in infant RCTs (crossover design): Immediate withdrawal of PPIs may trigger a rebound effect of hypersecretion of gastric acid, thereby influencing study ... was diagnosed in 12.3% of North American infants & in 1% of other pediatric age groups, with health care costs ~US $2386 /patient /6 months • Use of PPIs for the treatment of GERD in children has ... Using a reflux questionnaire for the inclusion of patients without other tools to diagnose GERD may not be of good value in the prediction of severity of GERD • Fourth: the studies involved children...

Ngày tải lên: 14/11/2016, 06:19

... pro-domain region of matrix metalloproteinases J Biol Chem 269, 30227–30231 Odake, S., Morita, Y., Morikawa, T., Yoshida, N., Hori, H & Nagai, Y (1994) Inhibition of matrix metalloproteinases by peptidyl ... substrate-degrading capacity of intact plasminogen (3 lg) activated by t-PA (100 ng) in the presence of collagen type IV cofactor, or by cell membrane-associated PAs (equivalent of 100 ng scuPA) but ... final volume of 200 lL with or without the MMP activator APMA (1 mM) or with or without EDTA (15 mM) Assays were incubated at 37 °C for 24 h All assays were performed in the presence of plasmin...

Ngày tải lên: 31/03/2014, 09:20

... source of proinflammatory cytokines and proteinases TNF-a is a key cytokine, which triggers the inflammatory cascade and stimulates the production of matrix degradable proteinases such as matrix metalloproteinases ... difficult to compare our findings with past cases due to lack of reports with detailed histopathological analyses of SAPHO syndrome synovitis, the similarity of the histopathological features ... pathogenesis of SAPHO syndrome synovitis through the processing of TNF-a, which triggers a cascade of pathological events through a mechanism similar to RA To the best of our knowledge, 10 cases of SAPHO...

Ngày tải lên: 11/08/2014, 14:21