Dual roles of transcription factor zic3 in regulating embryonic stem cell pluripotency and differentiation

... specification of midbrain dopamine neurons Radial glia cells are neuronal progenitors in vivo 10 11 14 1. 3 Long non- coding RNAs in biology 15 1. 3 .1 1.3.2 1. 3.2 .1 1.3.2.2 1. 3.2.3 Long non- coding RNAs in pluripotency ... pluripotency, and repressing differentiation simultaneously   18   1. 3.2 Long non- coding RNAs in neural developmen...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... chr 13: 36 ,31 5,670 -36 ,31 7,9 83 chr4:1,179,572-1,181,6 03 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 AK124684 AK125262 AK12 539 2 AL 832 189 AY927 532 BC008027 BC 030 122 BC 031 955 ... location (NCBI36/hg18) AF242771 AK05 533 5 AK056826 (lncRNA_ES1) AK0911 13 AL117559 AL 833 138 BC02 630 0 (lncRNA_ES3) chr12: 63, 376, 238 - 63, 376,597 chr19: 43,...

Ngày tải lên: 09/09/2015, 17:54

... was confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression ... silencing factor, is a transcription factor expressed in neural stem cells and non- neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds up...

Ngày tải lên: 09/09/2015, 17:54

... established in the mouse model system, and very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic ... B., Liu, M., and Shi, T (2011) Comparative analysis of human protein -coding and noncoding RNAs between brain and 10 mixed cell lines by RNA-Seq PLoS...

Ngày tải lên: 09/09/2015, 17:55

... as in embryonic carcinoma (EC) cells, which may have conserved roles in mammalian pluripotent stem cells (2) microRNAs expressed specifically in ESCs but not in other cells including EC cells These ... putative minimal promoter The number and precise sequences of the pre -microRNAs, their distance from the promoter and the polyadenylation sites, the regions flank...

Ngày tải lên: 12/09/2015, 08:16

... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... forebrain, midbrain, hindbrain and the spinal cord This figure was reproduced from Epstein et al., 1999 2.4 SHH and neural development During the initial ph...

Ngày tải lên: 11/09/2015, 10:14

... (m, 22 H), 0. 92 (t, 3H); 13C NMR (300 MHz, C6D6) δ 154.8, 94.6, 85.6, 80 .2, 79.7, 79 .2, 65.0, 64 .2, 63.8, 63 .2, 62. 8, 61.9, 32. 0, 29 .8, 29 .6, 29 .4, 29 .2, 28 .9, 28 .7, 28 .0, 25 .8, 25 .4, 23 .2, 22 .7, ... 64.44% 72. 98% 63.88% Structures of six obtained compounds (analogues of sphingosine) 2. 2 .2 Compounds Inhibitory Function on SPHK Activity in vitro 2. 2...

Ngày tải lên: 11/09/2015, 16:06

... 3. 1 .3 Staining 3. 1 .3. 1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the ... difference compared with the induced cells without inhibitors Interestingly and similarly with the hFOB test, 1μM of DMS and 5μM of CP6 inhibited the differentiatio...

Ngày tải lên: 11/09/2015, 16:06

... penetrating the cells and act on, as yet unknown, intracellular targets capable of triggering GPCR-independent calcium signals 4. 2.3 .4 MAPK (P42 /44 ) Activation and IκB Phosphorylation MAPK (P42 /44 ) ... was added into the cells and incubated for different times The results are summarized in Figure 4. 13A, Figure 4. 13C, and Figure 4. 13D 146 Chapter Role of S1P...

Ngày tải lên: 11/09/2015, 16:06

... suggests the potential for the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including ... osteogenic differentiation in most of the cases, while at higher amounts (1μM DMS and 5 M CP6) showed to inhibit the osteogenic differentiation in mo...

Ngày tải lên: 11/09/2015, 16:06

... messenger in cell proliferation induced by PDGF and FCS mitogens." Nature 365 (64 46) : 557 -60 Olivera, Ana, Nicole Urtz (20 06) “IgE-dependent Activation of Sphingosine Kinases and and Secretion of Sphingosine ... N, N-dimethyl-derythro -sphingosine- induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Ot...

Ngày tải lên: 11/09/2015, 16:06

... clinical usage Meeting this increasing need for more MSCs is one of the main motivations of the present study, in particular, in studying the role of S1P in adult stem cell proliferation Another ... maintenance and differentiation In particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness mainte...

Ngày tải lên: 11/09/2015, 16:06

... role of REST and its cofactors in ES cells ………………110 3.1 Introduction 3.2 Result 3.2.1 The role of REST and its cofactors in ES Cells 3.2.1.1 Dynamic expression profile of REST cofactors in ES Cell ... 3.2.1.2 Depletion of REST cofactors in ES cells 3.2.2 Genome wide mapping of REST and its cofactors’ binding sites in ES Cells 3.2.2.1 Generation of Stable REST co...

Ngày tải lên: 14/09/2015, 14:05