expression function and regulation of transcription factor mef2 in neurons

... N-terminal half of the protein, including the dehydrogenase domain Specific binding of the FNR is mediated by a unique series of proline/serine-rich repeat motifs located in the C-terminus For the integration ... in protein–protein interaction are blue and the dehydrogenase domains of Tic32 and Tic62 are shown in green Tic110 contains two transmembrane domains at the proximal N-terminus The topology of ... (blue) Tic40 consists of an N-terminal transmembrane domain and a soluble C-terminus protruding into the stroma Conserved regions of the C-terminus are the TPR domain, consisting of seven predicted...

Ngày tải lên: 07/03/2014, 03:20

... element binding protein (SREBP-2), was identified in the first intron of the ABCA1 gene and was reported to be involved in the regulation of ABCA1 expression in the liver [21] Recently, MiR-33, an intronic ... role in the degradation of various proteins such as IjBa, associates with ABCA1 and controls the ubiquitinylation and deubiquitinylation of ABCA1 [36] Several protein kinases including protein kinase ... (PKA), protein kinase C (PKC), Janus kinase (JAK2) and casein kinase (CK2) are involved in the regulation of ABCA1 activity and stability by apoA-I The interaction of apoA-I with ABCA1 increases...

Ngày tải lên: 22/03/2014, 15:21

... performance of all basic methods in discriminating between binding sites for the transcription factor of interest and binding sites of other transcription factors; however, weighting each match ... left 35 transcription factors and 410 binding sites, with an average of 11.7 ± 8.5 sites per transcription factor 2.2.2 Approaches for representing and searching for transcription factor binding ... important and timely, as whole-genome scans in search of the binding sites of a particular protein are increasingly used to make functional annotations of uncharacterized proteins, and to infer properties...

Ngày tải lên: 13/11/2014, 09:10

... maprotiline and another TCA, amitriptyline Antidepressant treatment reduced pain behavioral responses indicating antinociceptive effect of maprotiline and amitriptyline treatment in a model of inflammatory ... reuptake inhibition activity, will induce iPLA2 expression leading to increased DHA levels and subsequent production of resolvins via a concurrent increase in 15-LOX expression The increase in DHA and ... sometimes 2.1 Orofacial pain Orofacial pain is defined as “pain within the structures of the oral cavity and face, usually of a diffuse pattern” (Zwemer, 1998) Orofacial pain includes pain disorders...

Ngày tải lên: 09/09/2015, 08:15

... Cul3-Cul3 binding is independent of cullin neddylation in vivo 90 5.10 Cul3-Cul3 binding is independent of cullin neddylation in vivo 91 5C The structure of CRL3 dimer 92 5.11 Two Cul3 proteins are involved ... 1.10 CAND1 CAND1 (Cullin-Associated Neddylation Dissociated 1) is a 120kDa cullin-binding protein It interacts directly with both the cullin N-terminus and C-terminus The CAND1 protein was initially ... N-terminus inhibits the binding of the Skp1 adaptor and the substrate-recognition subunit F-box proteins to Cul1 On the other hand, upon binding of the CAND1 N-terminus ! 28! and the Cul1 C-terminus,...

Ngày tải lên: 10/09/2015, 09:04

... regulatory chain motifs48 The 12 Figure Functional domains of a transcription factor The amino acid sequence of a prototypical transcription factor is illustrated, containing a DNA-binding domain (DBD), ... 10 Gain- and Loss -of -function phenotypes of Oct4, Nanog and Sox2 in ES Cells Loss -of -function phenotypes are described in the left column, and grain -of -function phenotypes are described in the ... stimuli and increased stability of gene expression in ES cells In addition to transcription factor binding targets, a recent study was conducted to determine protein interaction patterns of key...

Ngày tải lên: 11/09/2015, 09:02

... MOLECULAR FUNCTION AND REGULATION OF THE BAX-ASSOCIATING PROTEIN MOAP-1 Fu Nai Yang INSTITUTE OF MOLECULAR AND CELL BIOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2007 MOLECULAR FUNCTION AND REGULATION OF ... domain in Bak which is completely distinct from previously defined BH1 and BH2 domains among the Bcl-2 family proteins (Yin et al., 1994) mediates cell death and protein binding functions of Bak ... oxidoreductase function Interestingly, knockout of AIF in human tumor cells has led to the surprising finding that AIF suppresses chemical stress-induced apoptosis in certain cellular contexts instead of...

Ngày tải lên: 14/09/2015, 12:54

... against a sequence between the N-terminal and the first transmembrane domain of the protein of the human and rat CB2 receptor The specificity of the CB1 and CB2 antibodies was described in McIntosh ... nonspecific interactions is supported by the observation that preabsorbing of the CB1 antibody with its corresponding blocking peptide eliminated almost all of the staining of these bands (Fig ... higher than those of anandamide In fact, after LPS-induced maturation, the amounts of 2-AG were increased 2.8-fold, as in the mouse macrophage J774 cell line [24] and in rat circulating macrophages...

Ngày tải lên: 22/02/2014, 07:20

... from the beginning of intron and the novel microsatellite, which included a tandem repeat of (TC)n is located approximately at 1264 bp from the beginning of intron 3.3 Allele frequencies in different ... SJ158 and a novel microsatellite locus within introns but not the SJ159 locus The presence of SSRs within introns has been documented in a number of species and they are mainly composed of monomers ... traits in the pig ACKNOWLEDGEMENTS We would like to thank teachers and graduate students at the Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture for managing and slaughtering...

Ngày tải lên: 14/08/2014, 13:22

... transfected into CHO cells with lipofectamine The expression of OVA and Fc was determined by flow cytometry, Western blotting, and enzyme-linked immunosorbent assay (ELISA) DNA sequencing and restriction ... effective in preventing allergen-induced airway inflammation in a murine allergic model OVA-sensitization and challenge could induced the development of airway inflammation and Th2 responses and suppressed ... both activating and inhibitory FcRs, and the balance between activating and inhibitory signaling will determine whether uptake of immune complexes results in naive T cell activation and protective...

Ngày tải lên: 14/08/2014, 19:22

... knockdown in activated microglia altered the expression of proinflammatory cytokines such as TNF-α and IL-1β and inflammatory mediators including iNOS and NO and the migratory and phagocytic ability of ... cytochromes and P-450 enzymes, xanthine oxidase and iNOS (Wilkinson and Landreth, 2006) The activated microglia exhibit increased expression of iNOS and NADPH oxidase which are involved in inflammatory ... protein-4 (CRMP4) - Gene Ontology predictions The molecular function of Dpysl3 includes SH3 binding domain, chondroitin sulfate binding domain (Franken et al., 2003), phosphoprotein binding domain...

Ngày tải lên: 12/09/2015, 11:24

... Role of nuclear actin-binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain Actin-binding protein Targeting sequence Gelsolin Direct or indirect ... Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ()) Cross-linking proteins Functions as scaffolds for signaling intermediates that stimulate actin elongation; binding partners ... actin is a component of pre-mRNP particles, and is incorporated into pre-mRNAs by binding to a specific subset of RNA-binding proteins [4,22]; and (h) actin is a component of PICs and depletion of...

Ngày tải lên: 07/03/2014, 01:20

... von Willebrand factor staining) Page of 10 (page number not for citation purposes) Figure Autocrine activation of cortisol and the regulation of fibroblast function of fibroblast function Bone ... Ross E, Bofill M, Martin S, Salmon M, Buckley CD: Global gene expression profiles in fibroblasts from synovial, skin and lymphoid tissue reveals distinct cytokine and chemokine expression patterns ... defined In studies presented here we examined site-specific expression of 11βHSD1 in fibroblasts and show that the resulting variations in glucocorticoid metabolism play an important role in...

Ngày tải lên: 09/08/2014, 08:22

... leucocyanidins Tawny Naringenin 5’ OH Eriodictoyl leucodelphinidin DFR leucopelargonidin leucocyanidin ANS cyanidins LAR delphinidin pelargonidin cyanidin UF3GT (activated by R) ANR anthocyanins Delphinidin-3-glycoside ... 3’ position of cyanidin and delphinidin (recently reviewed in [13], Figure 1) This glycosylation is thought to increase the stability and solubility of the cyanidin molecule [14] In lines with ... color in hilum and spreading slightly beyond the hilum) >i (seeds completely black) Inhibitor acts in a dominant, gain -of -function manner with maternal-effect inheritance, and results in seed...

Ngày tải lên: 11/08/2014, 11:21

... domain affects the binding affinity [15,16] WRKY proteins belong to a super-family of zinc finger proteins [WRKY-Glial Cell Missing (GCM1)] containing six members [22] For example, genes coding ... Ohashi Y: Expression of the Gene for a Small Gtp-Binding Protein in Transgenic Tobacco Elevates Endogenous Cytokinin Levels, Abnormally Induces Salicylic-Acid in Response to Wounding, and Increases ... amino acids in length In 11 BnWRKY TF proteins, we identified two separate WRKY domains (Additional file 3), and both N- and C-terminal WRKY domains of these proteins were included in the phylogenetic...

Ngày tải lên: 12/08/2014, 03:20

... Predicted and aligned binding sites in pCRMs Predicted and aligned binding sites in pCRMs Predicted binding sites and aligned binding sites (see Materials and methods) in positive, ambiguous and negative ... clustering should provide a valuable way of distinguishing functional and non-functional binding-site clusters in the D melanogaster genome explained by the positional conservation of sites found in ... ortholog The location of binding sites in D melanogaster, binding sites in D pseudoobscura and aligned binding sites along with the average density of sites (700-bp windows) are shown in the bottom three...

Ngày tải lên: 14/08/2014, 14:21

... free energy of binding for all possible bases at each position in a binding site [9] The algorithm then integrates over the affinities of all possible binding sites within a region of interest by ... weight function κC GOMER assumes that the intrinsic binding affinity of the dimer, Ka,XY,i,j, is the product of the binding constants of the two proteins, X and Y, for their respective sites, i and ... consists of windows i and j; it is analogous to Ka,eff,X,i, the equilibrium constant for monomeric X binding to site i in that it is the product of an intrinsic binding affinity, Ka,XY,i,j, and a...

Ngày tải lên: 14/08/2014, 14:22

... transcription of noncoding messengers (TIN and PIN transcription) within protein-coding genes Guided by this information, we designed a 44 k intron-exon oligoarray combining randomly selected protein-coding ... distribution of average intensities in the Figure of following page) Frequency(seeexon skipping and abundance of wholly intronic noncoding transcription in RefSeq genes Frequency of exon skipping and ... tissues and a few were only expressed in one The 'Regulation of transcription' GO category includes genes encoding various DNAbinding proteins such as transcription factors, zinc fingers and nuclear...

Ngày tải lên: 14/08/2014, 20:22

... to the region between )206 and )187 bp of the hsp70 promoter in vitro and in vivo, resulting in the induction of Hsp70 expression STAT3 is required for the induction of hsp70 promoter activity ... N-terminal ATPase domain binds ADP ⁄ ATP, and can hydrolyze ATP; the central b-sheet domain directly binds peptide substrates; and the C-terminal a-helix domain regulates substrate binding [4–6] ... three independent experiments, and the asterisks indicate significant differences at P < 0.01 promoter regions of hsp genes [19] In addition, an in ammatory cytokine, interleukin-6, induces the expression...

Ngày tải lên: 18/02/2014, 06:20

... using antiFLAG immunoglobulin As shown in Fig 1C, transfection of 10 ng of FLAG-DEC1 expression vector and 50 ng of FLAG-BMAL1 expression vector resulted in adequate levels of expression The transcriptional ... activity of DEC1 Determination of the binding domain of DEC1 to BMAL1 The interaction of DEC1 and BMAL1 was previously demonstrated by a yeast two-hybrid assay [7], and this interaction may be involved ... suppressive activity against CLOCK/BMAL1-induced transcription In addition, the N-terminal region of DEC1, including the bHLH domain, interacted with the C-terminal region of BMAL1 in a mammalian two-hybrid...

Ngày tải lên: 19/02/2014, 16:20