Light sheet based fluorescence correlation and cross correlation spectroscopy for quantitative measurements of bio molecules in live cells

... LIGHT SHEET BASED FLUORESCENCE CORRELATION AND CROSS -CORRELATION SPECTROSCOPY FOR QUANTITATIVE MEASUREMENTS OF BIO- MOLECULES IN LIVE CELLS ANAND PRATAP SINGH (M.Sc Chemistry, BHU, INDIA) ... enzyme-kinetics and ligand-receptor stoichiometry [121, 122] and in vivo protein-protein interaction in live cells and even in live organisms [33, 123...

Ngày tải lên: 09/09/2015, 11:19

... junction barrier integrity (7) Conversely, activation of atypical PKC isoforms using bryostatin instead increased the expression of claudin-1 in the tight junction complex, and increased tight ... cell motility We have previously shown that increasing the levels of CLDN-1 increases the invasion of melanoma cells (4) To determine if the increase in claudin-1 needs...

Ngày tải lên: 03/11/2012, 11:17

... The alkaline sedimentation profiles of HeLa and CCRF cells after hypoxia and reoxygenation already revealed [4] that hypoxic incubation caused significant accumulation of initiation competent replicons, ... gassing first mitotic cells appear after about 13 h, their number increases within the next h and decreases again at longer incubation A similar increase of DNA synt...

Ngày tải lên: 21/02/2014, 00:20

... (DEA) software (Montreal, Quebec) Average diameter of brachial artery (before and after dilation) was obtained from nine sequential images taken at rest and 12 taken during peak artery dilation ... L, Shaw AG, Busfield F, Haluska B, Barnett A, Kesting J, Short L, Marczak M and Shaw JT: Carotid artery intimal medial thickness, brachial artery flow-mediated vasodilation...

Ngày tải lên: 10/08/2014, 05:21

... THE STUDY OF INTERACTIONS OF TRANSMEMBRANE RECEPTORS AND INTRACELLULAR SIGNALING PROTEINS IN LIVE CELLS BY FLUORESCENCE CORRELATION AND CROSS -CORRELATION SPECTROSCOPY LIU PING (M.Sc), CAS A THESIS ... C-terminus domain, to expose binding sites for Src-homology-2 (SH2) domain-containing proteins and phosphotyrosine-binding (PTB) domain-containing...

Ngày tải lên: 14/09/2015, 14:25

... Figure Direct Vpr-Vpr interaction in HeLa cells visualized by FLIM Direct Vpr-Vpr interaction in HeLa cells visualized by FLIM Cells were transfected with the DNA construct encoding eGFP or eGFP-Vpr ... Sensitivity of CFP/YFP and GFP/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells Microsc...

Ngày tải lên: 13/08/2014, 05:21

... extended to the model determination of EGFP labeled proteins in living zebrafish embryos FCS has then been employed to investigate in vivo EGFP tagged Wnt3 (Wnt3EGFP) secretion and diffusion ... proteins remains problematic In this study, a Bayesian model selection approach has been applied to evaluate FCS data of fluorescent proteins in vitro and in vi...

Ngày tải lên: 09/09/2015, 08:12

... Characterization of Diffusion Behavior of a Novel Extra- cellular Sphingolipid Associated Peptide Probe by Fluorescence Correlation Spectroscopy and Imaging Total Internal Reflection Fluorescence ... cell membrane organization traced by SBD, two new biophysical tool Imaging Total Internal Reflection -Fluorescence Correlation Spectrosc...

Ngày tải lên: 12/09/2015, 09:55

... fiber cells. We finally analyze the FCS data with the anomalous subdiffusion  model and study the molecular crowdedness of cells in living embryos.   Chapter 5 describes the direct quantification of protein‐protein interactions in living zebrafish embryos with  ... use  in zebrafish embryos and demonstrate  several  applications of FCS in living zebrafish embryos,  showing that single...

Ngày tải lên: 14/09/2015, 08:41

... dynamics including diffusion, local concentration, and binding rate (67; 68) Scanning FCS Since traditional point FCS is more suitable for fast-diffusing molecules, in case of slow-diffusing or ... Fluorescence correlation microscopy (FCM), a technique term first introduced in 1995 by Terry and coworkers (93) which combines imaging techniques with FCS, identified points of int...

Ngày tải lên: 14/09/2015, 14:13

... Proposed video and transport features for different applications with performance in terms of delay and average PSNR for different RLC-PDU loss rates and QCIF video sequence Foreman coded at 7.5 fps Video ... account multiple users in a wireless system are not further discussed For some specific video- related issues and system design of schedulers and network buffe...

Ngày tải lên: 22/06/2014, 23:20

... and cell membrane organization investigated by imaging fluorescence cross‐ correlation spectroscopy. " Biophys J 97(9): 2630‐9.          I    Investigation of Peptide Lipid Interaction by Fluorescence Correlation Spectroscopy ... provides further guidelines for the rational design of antimicrobial peptides.      VIII        Investigation of Peptide Lipid Interaction by Fluorescence Correla...

Ngày tải lên: 11/09/2015, 10:05

... products labeled with the same fluorescent dye, the only way of differentiating the product from the reactant is when the product has a molecular mass that differs from the reactants by at least a factor ... of the dual-color complexes formed This easily distinguishes the products from the free reactants via the amplitude of the CCF, as compared to the weak depe...

Ngày tải lên: 14/09/2015, 10:36

... communication systems In smart antenna systems, the direction of users is an important factor to increase the capacity, and an antenna array is usually used at the base station to estimate and track ... hopping signals, and a new method is proposed to track multipath frequency hopping signals In Chapter 2, several popular methods for DOA estimation are addressed in...

Ngày tải lên: 15/09/2015, 22:51

... Summary The objective of this study is to apply biophysical fluorescence technique, i.e fluorescence correlation spectroscopy (FCS), in situ in the central nervous system of fruit fly (Drosophila melanogaster) ... neuronal membrane fluidity was being studied in situ in the central nervous system of Drosophila melanogaster embryos by FCS Our...

Ngày tải lên: 12/10/2015, 17:36