measurements of cell death in neuronal and glial cells

... ANT1-silencing induces glioblastoma cell death A Fig Analysis of ANT1 and ANT2 expression in nontransfected ADF cells and in ANT1- and ANT2-silenced ADF cells (A) Number of ANT1 and ANT2 transcripts in ... siRNA + puromycin Fig Effect of ANT1 and ANT2-silencing on ADF cells (A) Cell growth curves analyzed by crystal violet assay in nontransfected ADF cells, and in Scramble- and ANT1 siRNA and ANT2 siRNA ... assay in nontransfected U87-MG cells, and in Scramble- and ANT1 siRNA U87-MG-transfected cells Each point represents the average of two experiments performed in triplicate The number of live cells...

Ngày tải lên: 29/03/2014, 09:20

... understanding of cell- death events after ionizing irradiation and development of molecular targets for cancer treatment Results To examine the kinetics of the apoptotic cell death induced by ionizing ... were introduced into the cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations Quantification of cell death Apoptosis was investigated by Annexin V labeling ... Role of JNK in radiation-induced mitochondrial cell death M.-J Kim et al Fig Ionizing radiation induces expression of Fas and activation of caspases in human cervical cancer cells (A) Ionizing...

Ngày tải lên: 07/03/2014, 05:20

... Determination of Brain Death in Infants and Children Critical Care Medicine 2011;39:2139–2155 Nakagawa TA, Ashwal SA, Mathur M, Mysore M Guidelines for the Determination of Brain Death in Infants and ... has resulted in irreversible coma Coma and apnea must coexist to diagnose brain death Determination of brain death by neurologic examination should be performed in the setting of normal age-appropriate ... Determination of Brain Death in Infants and Children:An Update of the 1987 Task Force Recommendations— Executive Summary Thomas A Nakagawa, MD, FAAP, FCCM 2.Report of Special Task Force Guidelines...

Ngày tải lên: 14/11/2016, 06:25

... entry and the induction of cyclin D1 expression JunB inhibits G1 to S phase progression by inducing p16 and repressing cyclin D1 JunD inhibits S phase entry and increases the numbers of resting cells ... N-terminal kinase) family of MAP kinases by specific phosphorylation of serine 63 and 73 of c-Jun within its transactivation domain, resulting in a large increase in the ability of c-Jun to interact ... patterns of various chromogranin genes 97 Fig 4.9 Basal and NO-inducible SgII protein levels in SH-Sy5y and TAM67 cells 98 Fig 4.10 Increased NO-resistance of TAM-67 stable cells over-expressing...

Ngày tải lên: 16/09/2015, 15:54

... BCR–ABL in inter6056 leukin-3-dependent Baf-3 cells represses BIM and allows interleukin-3–independent survival; treatment of these cells with imatinib reverses the effects of BCR– ABL, resulting in ... cells [64] and in BCR–ABL+ K562 CML cells in response to both imatinib and nilotinib [65] INNO-406, a more potent inhibitor of BCR–ABL, also induces apoptosis in BCR–ABL+ K562 cells, and whilst ... loss of ERK1 ⁄ and ⁄ or PKB signalling and, as a consequence, promote increased expression of BIM and BIM-dependent cell death in tumour cells Here we review recent advances in understanding BIM...

Ngày tải lên: 16/03/2014, 00:20

... activation of Aur-A and induces monopolar spindle in NB4-R2 cells (A) VX-680 inhibited phosphorylation of AurA at Thr288 in NB4-R2 cell line Cells were incubated with increasing doses of VX-680 ... defects of mitotic spindle in VX-680 treated cells VX-680 suppresses cell growth and induces cell apoptosis in NB4-R2 cells Next, we studied if VX-680 could suppress proliferation in NB4-R2 cells in ... proliferation of leukemic cells in AML and inhibition of activation of Akt can result in suppression of cell growth [40,41] In the present study, phosphorylation of Akt-1 and GSK3b, the downstream of Akt-1,...

Ngày tải lên: 18/06/2014, 19:20

... of oxidative and inflammatory insults The positive feed back loop may continue until the additional neuronal death (~50% of the remaining of the initial death) combined with the initial loss ... Boehringer-Mannheim, Indianapolis, IN) or βactin (Sigma, St Louis, MO) antibodies for detection of the level of dopaminergic neuronal terminals, and for normalization of the loading protein The ... MPTP-induced loss of THpositive neurons and neuronal terminals in the nigrostriatal system Because of possible unintended confounding effects associated with oral administration of COX-2 inhibitors...

Ngày tải lên: 19/06/2014, 22:20

... activation of Aur-A and induces monopolar spindle in NB4-R2 cells (A) VX-680 inhibited phosphorylation of AurA at Thr288 in NB4-R2 cell line Cells were incubated with increasing doses of VX-680 ... defects of mitotic spindle in VX-680 treated cells VX-680 suppresses cell growth and induces cell apoptosis in NB4-R2 cells Next, we studied if VX-680 could suppress proliferation in NB4-R2 cells in ... proliferation of leukemic cells in AML and inhibition of activation of Akt can result in suppression of cell growth [40,41] In the present study, phosphorylation of Akt-1 and GSK3b, the downstream of Akt-1,...

Ngày tải lên: 20/06/2014, 03:20

... Briefly, following induction of apoptosis, cells were fixed in 1% paraformaldehyde and incubated with Br-dUTP in the presence of TdT enzyme, which results in the incorporation of Br-dUTP into exposed ... susceptibility to FasL-induced cell death, and TNF-α prevented these cells from undergoing FasL-induced cell death in a dose-dependent manner No such inhibitory effects of TNF-α on FasL-induced apoptosis ... fibroblast-like cells of mesenchymal origin that constitute the cellular component of periarticular tendons They play an important role in producing extracellular matrix and in initiating regenerative...

Ngày tải lên: 09/08/2014, 01:23

... Bcl-xL and Bcl-2 in LNCaP and PC3 cells, whereas ionizing radiation triggered a decrease in the levels of Bcl-2 in both cell lines Moreover, ErPC3treatment decreased the levels of Mcl-1 in LNCaP cells ... ErPC3 and ionizing radiation PC3 cells (A), DU145 cells (B), and LNCaP cells (C) were treated with increasing concentrations of ErPC3 (1-100 µM) and ionizing radiation (2, 5, 10 Gy) alone or in ... despite reducing the number of viable cells in the WST-1 assay, ionizing radiation did not induce significant apoptotic nuclear fragmentation in LNCaP cells (Figure 2D) In line with these findings,...

Ngày tải lên: 09/08/2014, 09:20

... replicated in flow-cytometry analyses of infected A549 cells (Fig 6a and 6b) 40 Figure growth of L pneumophila in A549 cells Intracellular Intracellular growth of L pneumophila in A549 cells A549 cells ... infection and intracellular growth of L pneumophila in A549 cells Intracellular growth of the virulent strain was observed day after infection, subsequently increasing to approximately 100-fold the initial ... fragmentation is shown in green (FITC staining), and A549 cell nuclei in red (PI staining) cells infected with the virulent strain of L pneumophila (Fig 8) Inhibition of nuclear DNA fragmentation,...

Ngày tải lên: 12/08/2014, 15:21

... apoptotic cells and thereby prevent the release of noxious intracellular compounds Although apoptosis and necrosis have been considered distinct and independent phenomena, the two modes of cell death ... immunosuppression in prolonged sepsis in at least two major fashions: the elimination of key effector cells and, alternatively, the capacity of apoptotic cells to induce anergy and a shift to a Th2 -cell response ... apoptosis of gastrointestinal epithelial cells may compromise the integrity of the bowel wall, resulting in the translocation of bacteria or endotoxins into the systemic circulation A contribution of...

Ngày tải lên: 13/08/2014, 18:22

... during AIA in BHK cells (5 min) 149 Fig 3.58 Intracellular pH changes during AIA in BHK cells (10 min) 150 Fig 3.59 Intracellular pH changes during AIA in BHK cells (20 min) 151 Fig 3.60 Intracellular ... during AIA in BHK cells (30 min) 152 Fig 3.61 Intracellular pH changes during AIA in BHK cells (40 min) 153 Fig 3.62 Intracellular pH changes during AIA in BHK cells (50 min) 154 Fig 3.63 Intracellular ... during AIA in BHK cells (60 min) 155 Fig 3.64 Intracellular pH changes during AIA in BHK cells (90 min) 156 Fig 3.65 Intracellular pH changes during AIA in BHK cells (4 hrs) 157 Fig 3.66 Intracellular...

Ngày tải lên: 11/09/2015, 16:07

... the knockdown of Bubr1 and Mad2 in H1299 cells 103 Fig 3.26: Reduction in percentage of population of taxol treated cells undergoing apoptosis in p73, Bubr1 and Mad2 knockdown cells 104 Fig 3.27: ... during mitosis, thus allowing chromatids to separate during anaphase towards opposing poles In the cells, the spindle checkpoint is kept active through the binding of inhibitory proteins BubR1 and ... of cell lines 28 39 39 39 39 ii 2.2.1.3 Subculturing of cells 2.2.1.4 Cryopreservation of cells 2.2.1.5 DNA transfections 2.2.1.6 siRNA transfections 2.2.1.7 Synchronization and released of cells...

Ngày tải lên: 13/09/2015, 21:21

... are commonly activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible ... glutamine, cysteine, and glycine It is the most abundant intracellular small molecule thiol present in mammalian cells and it serves as a potent intracellular antioxidant protecting cells from toxins ... gene encoding the chimeric protein of caspase-8 and the ligand-binding domain (LBD) of ERα and introduced the gene into breast cancer cell lines (Zhao et al, 2011) Upon administration of estrogen,...

Ngày tải lên: 23/03/2014, 17:20

... evaluation of the role of insulinlike growth factors (IGF) and of type-I IGF receptor signalling in hepatocarcinogenesis and in the resistance of hepatocarcinoma cells against drug-induced apoptosis ... markers of autophagy, such as induction of LC3 and conversion of LC3-I to LC3-II [48,49,118], induction of beclin [48], induction of BNIP-3 [66] and activation of G-interacting protein (GAIP) ... dihydrocapsaicin [118] in WI38 lung fibroblasts, cadmium in mesengial MES-13 cells [69,113] and TNFa treatment in MCF-7 [49] and L929 cells [48] Interestingly, in human ovarian cancer cells, cytoplasmic...

Ngày tải lên: 29/03/2014, 08:20

... proteins, including paxillin, p130Cas, vinculin and talin in focal adhesions These protein complexes anchor the actin cytoskeleton and provide structural integrity to cells [16] The importance of ... A2058 cells, cells were pretreated with either tyrosine kinase inhibitor, genistein, or MAP kinase inhibitor, PD98059 Inhibition of tyrosine kinase by genistein greatly inhibited LPA-induced PAK ... stimulate cell spreading and motility in neuroblastoma cells [34] It is possible that different small G proteins are involved in LPA-induced cell motility in different cell types LPA stimulates tyrosine...

Ngày tải lên: 30/03/2014, 13:20

... from extracts of non -neuronal COS-7 cells indicating that processing of hproNPFFA at a specific nonbasic residue should be considered to explain the presence of NPFF in cell extracts In contrast, ... production of NPFF should be considered Clearly COS-7 and SH-SY5Y cells generate different proNPFFA fragments indicating that a neuronal cell line possess a capacity of processing not found in non -neuronal ... investigated the processing of proNPFFA in SH-SY5Y human neuroblastoma cells and COS-7 (nonneuronal) cells transiently transfected with the human proNPFFA cDNA The pattern of intracellular NPFF-related...

Ngày tải lên: 31/03/2014, 07:20

... are commonly activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible ... glutamine, cysteine, and glycine It is the most abundant intracellular small molecule thiol present in mammalian cells and it serves as a potent intracellular antioxidant protecting cells from toxins ... gene encoding the chimeric protein of caspase-8 and the ligand-binding domain (LBD) of ERα and introduced the gene into breast cancer cell lines (Zhao et al, 2011) Upon administration of estrogen,...

Ngày tải lên: 27/06/2014, 11:20

... induces apoptotic and necrotic cell death in CCRF-CEM cells GPS induces apoptotic and necrotic cell death in CCRF-CEM cells CCRF-CEM cells were exposed to various doses of GPS (1, or puffs) and ... The cells were resuspended at × 106/ml in pre-warmed JC-1 working buffer containing M JC-1 and incubated for 15 in a 37°C/5% CO2 incubator Subsequently, the cells were washed in assay buffer and ... (3 and puffs) were not significantly different (p < 0.62) FACS analysis of Annexin V/PI-stained cells To determine the mode of cell death upon GPS treatment, cells were stained with AnnexinV/PI...

Ngày tải lên: 12/08/2014, 14:20