image correlation spectroscopy for microrheology

... target for the management of sepsis and its associated complications in critically ill patients xi LIST OF TABLES Table 1.1 Diagnostic criteria for sepsis 56 Table 1.2 Standard definitions for sepsis ... important mechanism of H2S degradation, and therefore accounts for a smaller amount of H2S Additionally, H2S can be scavenged by methemoglobin to form sulfhemoglobin or consumed by metallo- or ... quality graduate education that will put me on the forefront of global competition For that, I am truly thankful to NGS for i providing me with a generous scholarship as well as ample opportunities...

Ngày tải lên: 10/09/2015, 08:32

... pET19b(H6bG), as described above for glycerol dehydratase staining for glycerol dehydratase was performed as described previously for diol dehydratase [32] The apparent molecular weight of the enzyme ... analysis of the enzyme Substrate-free enzyme used for measuring Km values for substrates was obtained by gel filtration on Sephadex G-25Ò or dialysis for days against 500 volumes of buffer A with ... changes One-minute assay was employed for measurement of Km for glycerol, as glycerol induces suicide inactivation of the enzyme [39] Apparent Km values for substrates and AdoCbl were determined...

Ngày tải lên: 21/02/2014, 03:20

... anti-TNF agents were used as controls for the immunological study Patients had not received steroids for at least 24 hours before PB and BM sampling Written informed consent was obtained from all ... fluorochrome-conjugated monoclonal antibodies for 30 minutes on ice Combinations of anti-CD19, anti-HLA-DR, antiCD27, anti-CD38 and anti-CD45 staining were used for analysis of B cells For analysis of T cells, anti-CD3, ... performed IgG isotype controls were used in all experiments to determine the posi- Page of (page number not for citation purposes) At least 200,000 events were collected in the lymphocyte gate for...

Ngày tải lên: 09/08/2014, 14:22

... the correlation was highly significant, both for the HIV-specific stimulation (p = 0.0006, top) and for pooled HIV-specific and CMV-specific results (p = 0.0034, bottom) This argues strongly for ... blood sample from a healthy donor was incubated for six hours with pp65 peptide, in the presence of brefeldin A Phenotypic analysis was performed both before and at various time points during stimulation ... immunogenic proteins from HIV or CMV, then did a combined analysis for IFNγ and IL-2, as well as for CD27, CD28, and CD45RA Results for each possible phenotype of cytokine-positive cells were expressed...

Ngày tải lên: 10/08/2014, 05:20

... classification, and historical grading was performed according to WHO criteria Paraffin-embedded, formalin-fixed surgical specimens were prepared and collected for immunohistochemical staining The immunohistochemical ... vessels was counted in five high-magnification fields (×400) for each case The mean value for the five fields was calculated as the LVD for each tumor To evaluate the impact of LVD on prognosis, ... Cancer Res 2004, 64:554-564 24 Remmele W, Stegner HE: Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast...

Ngày tải lên: 10/08/2014, 10:21

... survival time for EGFR positive patients was 31 months, whereas the survival time was 48 months for patients with EGFR negative expression, with the latter significantly longer than the former (p ... was no correlation of EGFR and COX-2 expression with overall survival in 22 patients receiving chemotherapy alone (P > 0.05) Correlation of EGFR and COX-2 expression As shown in Table 7, no correlation ... blue line indicates the survival for EGFR negative and the green line represents survival for EGFR positive expression subgroups EGFR expression in tissue sample before surgery might be helpful...

Ngày tải lên: 10/08/2014, 10:21

... expression and clinical characteristics Page of Table (corrected table six) COX-2 expression and correlation with clinical features EGFR Positive expression rate P value Clinical features 0.448 ... Lymph node N0 10 90.90% 56.40% 22 N1-3 35 89.70% 0.006* *P < 0.05 Table (corrected table seven) Correlation of EGFR and COX-2 protein expression Number of cases COX-2 Positive rate(%) EGFR P value ... Yali, Xu Feng, Wang Jin: EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features Journal of Experimental & Clinical Cancer Research 2011, 30:27...

Ngày tải lên: 10/08/2014, 10:21

... μl N HCl and OD was read at 450/650 nm Correlation analyses were performed using GraphPad Prism software PAP248-286 and generated SEVI antibodies, AB performed CVL experiments, NDR generated testis ... in the vagina for about 24 hours [32] Thus, amyloidogenic PAP fragments may be generated over a period of several days Notably, 10% SE was most effective in infectivity enhancement for the first ... SE/virus mixture was incubated for 10 at RT, and 20 μl were added to 280 μl TZM-bl cells After different time points, the supernatant was removed, and fresh DMEM was added for further culture The star...

Ngày tải lên: 13/08/2014, 01:20

... steroid therapy for bronchial asthma In our patients, diagnosis of the sinobronchial syndrome was based on the following criteria: 1) productive cough on most days for at least months for consecutive ... Data are transformed to logarithmic values for cough threshold at this test A p-value of less than 0.05 was taken as significant Results Cough threshold to inhaled capsaicin before each treatment ... COX-2 for handling cough reflex sensitivity in asthmatic airway with chronic eosinophilic bronchial inflammation We, therefore, conducted this study using etodolac with potent affinity for the...

Ngày tải lên: 13/08/2014, 10:20

... O’Reilly books may be purchased for educational, business, or sales promotional use Online editions are also available for most titles (safari.oreilly.com) For more information, contact our corporate/institutional ... widget container with two UpdatePanels, one for the header area and one for the body area where the real widget is loaded However, for even better performance, what if the header UpdatePanel didn’t ... because clicking on a tab is a regular postback for ASP.NET But for the widgets that are loading for the first time on the new tab, it’s not a postback for them They will fail when trying to read...

Ngày tải lên: 15/11/2012, 14:24

... Do not configure loopback interfaces and routing protocol yet Step Save the configuration information for all the routers Why save the running configuration to the startup configuration? ... designated router? _ d Type the command show ip ospf neighbor detail for more information e What is the neighbor priority of 192.168.1.1 from router Brasilia? ... Configure the loopback interface on each router to allow for an interface that will not go down due to network change or failure This task is performed by typing interface loopback # at the global...

Ngày tải lên: 18/01/2014, 04:20

... is the basic starting point for cabling your equipment: (The following information will vary depending upon your ISDN simulator or actual ISDN lines) ISDN Information for Router1: isdn switch-type ... basic-ts013 ntt vn2 vn3 Page of TS013 switch type for Australia NTT switch type for Japan VN2 switch type for France VN3 and VN4 switch types for France router1(config)#isdn switch-type basic-ni1 ... switch-type ? basic-1tr6 1TR6 switch type for Germany basic-5ess AT&T 5ESS switch type for the U.S basic-dms100 Northern DMS-100 switch type basic-net3 NET3 switch type for UK and Europe basic-ni1 National...

Ngày tải lên: 18/01/2014, 04:20

... 73 Creating the Form Processor for User Registration 74 Displaying the Registration Form and Processing Registrations 81 Adding CAPTCHA to the User Registration Form ... Images on the Post Preview 401 Deleting Blog Post Images 403 Using Scriptaculous and Ajax to Delete Images 406 Deleting Images ... differences in code required for any of these platforms Note also that references to Linux can typically also include similar platforms such as FreeBSD and Mac OS X For Windows there are slight...

Ngày tải lên: 24/01/2014, 14:07

... process of reinforcement or punishment these slight differences will be magnified over time This is often proposed as a social explanation for the behavioral correlates of somatotype For example, ... from the three germ layers formed during gasturlation and his three components of physique were each named for the one of the germ layers; endoderm, mesoderm, and ectoderm For example, Sheldon argued ... toward criminal behavior The other proposition offers an evolutionary explanation for most forms of criminality and for why testosterone affects brain functioning to enhance criminal behavior (p...

Ngày tải lên: 13/02/2014, 16:20

... were cultured for 12 h after transfection with siHO1, siHO2, or siGAPDH, then treated with lM hemin for 12 h, and harvested for the measurement of heme content Cells were treated for 24 h with ... HeLa cells and HepG2 cells were cultured for 12 h after the transfection with siHO1, siHO2, or siGAPDH, then treated with lm hemin for 12 h, and harvested for heme measurement SiRNA and expression ... manufacturer’s protocol The amounts of each siRNA used were 40 pmol for HeLa cells and 80 pmol for HepG2 cells, cultured in a 9-cm dish for the indicated hours of incubation The effects of siRNA were...

Ngày tải lên: 19/02/2014, 05:20

... used for real time PCR are as follows For hGAPDH forward, 5¢-CAAAGTTGTCATG 1684 F T Grall et al GATGACC-3¢; reverse, 5¢-CCATGGAGAAGGCTGGG G-3¢, which will amplify 195 bp of human GAPDH For Cox2 forward, ... (Qiagen) PCR was performed using lL of a 60 lL DNA extraction in TE buffer with Hi-Fi Taq polymerase (Invitrogen) for 28 cycles (95 °C for 30 s, 52 °C for 30 s and 68 °C for min) The primers ... diluted : 10 in dilution buffer for immunoclearing with sheared salmon sperm DNA, normal rabbit serum and protein A–Sepharose for h at °C Immunoprecipitation was performed overnight at °C with 80...

Ngày tải lên: 19/02/2014, 17:20

... 73 Creating the Form Processor for User Registration 74 Displaying the Registration Form and Processing Registrations 81 Adding CAPTCHA to the User Registration Form ... Ajax to Delete Images 406 Deleting Images when Posts Are Deleted 411 Reordering Blog Post Images 412 Displaying Images on User ... differences in code required for any of these platforms Note also that references to Linux can typically also include similar platforms such as FreeBSD and Mac OS X For Windows there are slight...

Ngày tải lên: 19/02/2014, 20:20

... affinities for PKCa and their ability to induce translocation of PKC to cellular membranes These ligands are shown to induce different patterns and kinetics for translocation of PKC isoforms to ... experiments were performed for each DAG lactone, and the Ki values presented represent the means ± SEM of these triplicate experiments Measurement of translocation of GFP-tagged PKC isoforms a and d ... diacylglycerol derivatives were imaged using a 63· 1.4 NA Zeiss Plan-Apochromat oil immersion objective and with varying zooms (1.4–2) Time-lapse images were collected every 30 s before and after treatment...

Ngày tải lên: 15/03/2014, 09:20

... (numbers for HGDH) Lowercase letters indicate amino acid sequence insertions The residues discussed below are coloured red for arginine, blue for glutamate and aspartate, green for histidine, cyan for ... of HicDH (ternary complex in open conformation; colour code as for panel A) Atom colour code as for panel A and 2-oxoisocaproate in grey for carbons and red for oxygen atoms (D) Stereo display ... (Fig 4, and Fig 5A for a closer view) The residues lining the active site are all well defined except for Arg235, which displays residual electron density for two possible conformations, one pointing...

Ngày tải lên: 23/03/2014, 13:20