Local induction of heat shock proteins using magnetic fluid hyperthermia for ocular neuroprotection in glaucoma

... LOCAL INDUCTION OF HEAT SHOCK PROTEINS USING MAGNETIC FLUID HYPERTHERMIA FOR OCULAR- NEUROPROTECTION IN GLAUCOMA MINHONG JEUN A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... 7.4 141 Local Induction of HSPs 72 in RGCs by MFH 7.4.1 Optimization of concentration of EMZF@PEG SPNPs and holding time of AC heating stress for induction...

Ngày tải lên: 09/09/2015, 10:08

... 40 80 12 0 16 0 200 A 10 1 10 2 FL2-H 10 3 10 4 72Q 72Q+NAC Counts 40 80 12 0 16 0 200 10 0 10 1 10 2 FL2-H 10 3 10 4 B 10 3Q 10 3Q+NAC Counts 40 80 12 0 16 0 200 10 0 10 0 10 1 10 2 FL2-H 10 3 10 4 Fig Analysis of mitochondrial ... vector (72Q) or httEx1 -10 3Q-EGFP vector (10 3Q) Transfections were performed with a combination of those encoding Hsp70 ⁄ Hdj -...

Ngày tải lên: 19/02/2014, 06:20

... in the absence of OVA (No), or in the presence of 50 µg/mL OVA (ova), 50 µg/mL of OVA plus 5-200 µg/mL of respective heat shock proteins (Hsp/ova), 50 µg/mL of OVA plus 100 µg/mL of lactorferrin ... absence of OVA (No), or in the presence of 50 µg/mL OVA (ova), 50 µg/mL of OVA plus 5-200 µg/mL of respective heat shock proteins and 20 µg/mL PMB(Hsp/ova) , 50 µg/...

Ngày tải lên: 09/10/2015, 11:06

... inhibited pulmonary fibrosis in bleomycin treated rat lungs Analysis of HSP47 protein expression revealed a central role of HSP47 in the progression of bleomycin-induced pulmonary fibrosis In fact, ... HSP47 protein in the lung was increased during bleomycin-induced pulmonary fibrosis These results suggest that HSP47 is expressed during pulmonary fibrosis...

Ngày tải lên: 12/08/2014, 15:20

... structure of cytosolic class II small heat shock proteins Plant Physiol 114, 1477–1485 Basha, E & Vierling, E (2003) Cloning and sequencing of a new cytosolic class II small heat shock protein from wheat ... molecular chaperone activity of plant recombinant small heat shock proteins Methods Enzymol 290, 350–365 Helm, K.W., LaFayette, P.R., Nagao, R.T.,...

Ngày tải lên: 07/03/2014, 15:20

... oligomerization and Dynamics of bacterial sHsps (Eur J Biochem 271) 2501 Fig Hypothetical model on chaperone dynamics and chaperone substrate interaction of small heat shock proteins (sHsps) ... 45 and 38 kDa (B) Elution profile at 25 °C of a sample diluted from mgÆmL)1 (arrow) to 0.5 mgÆmL)1 elution of standard proteins was completely independent of temperature...

Ngày tải lên: 23/03/2014, 12:20

... mammalian a- crystallins, the oligomerization principles of bacterial a- Hsp proteins have received little attention Available data indicate that a- Hsp proteins from prokaryotes and plants may ... principles of a- Hsp assembly and the relation between complex formation and chaperone activity, we constructed a series of truncated and point-mutated a- Hsp varian...

Ngày tải lên: 31/03/2014, 21:21

... among the regulation of nucleolin ⁄ C23, the activation of caspase-3 and the induction of apoptosis under the setting of oxidative stress and Hsp70 overexpression More importantly, for the first ... Xiao X (2009) ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived...

Ngày tải lên: 16/02/2014, 09:20

... into the nucleus upon heat shock and may activate nonclassical heat shock genes, but does not activate classical heat shock genes (i.e HSP genes) HSF1 prefers long arrays of the nGAAn unit, while ... region of HSP genes Unlike yeast HSP genes, the DNase I hypersensitive region of the hsp70 promoter of nonheat-shocked cells contains paused Pol II, which is transcriptional...

Ngày tải lên: 18/02/2014, 04:20

... coronal section and scheme of the coronal section at E14.5 in (F) (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline Surprisingly, the ... binding is reduced, indicating that HSF4 facilitates HSF1 binding via chromatin remodelling Heat shock genes are not HSF4 targets, but HSF4 regulates a set of nonclassical h...

Ngày tải lên: 18/02/2014, 04:20

... Dementia, gliosis and expression of the small heat 2625 Small heat shock proteins and disease 120 121 122 123 124 125 126 127 128 shock proteins Hsp27 and [alpha]B-crystallin in Parkinson’s disease Neuroreport ... case in point, formation of huntingtin-containing inclusion bodies in Huntington’s disease encourages cell survival, whereas monomers and smal...

Ngày tải lên: 19/02/2014, 18:20

... Heat shock protection against cold stress of Drosophila melanogaster Mol Cell Biol 8, 35503552 Joplin KH, Yocum GD & Denlinger DL (1990) Cold shock elicits expression of heat shock proteins in ... processes involve expression of some Hsps in Drosophila [40,57] Finally, it has been suggested that expression of Hsps during recovery from cold might...

Ngày tải lên: 06/03/2014, 09:22

... Roles of the heat shock transcription factors in regulation of the heat shock response and beyond FASEB J 15, 1118–1131 Voellmy R (2004) On mechanisms that control heat shock transcription factor ... HSE-type specific recognition by human HSFs resulted in robust activation by hHSF4–VP16, without changing the magnitude of the heat shock response (cp3)...

Ngày tải lên: 07/03/2014, 00:20

... occurs on the transcriptional or on the translational level, the system of combined transcription translation was decoupled The inuence on the transcriptional level was investigated by in vitro transcription ... dissociation constants were derived for the inhibition of the protein expression by CSP using two models In the most simple model the inhibitio...

Ngày tải lên: 07/03/2014, 21:20

... Fig ATP-binding domain of HSP70 is essential for the inhibition of H2O2-induced activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins on the ... activation Such a mechanism is independent of the interaction of HSP70 with Smac but requires the ATP-binding domain of the protein However,...

Ngày tải lên: 16/03/2014, 01:20