identification of heat shock factor regulated genes and pathways

... CaHsf genes were significantly up -regulated, while 12 genes were unregulated Similarly, 11 CaHsf genes were unregulated by a Put treatment, and no gene was down -regulated Only five and four of the ... BJ Genome-wide identification, classification and analysis of heat shock transcription factor family in maize BMC Genomics 2011;12:76 Parsell DA, Lindquist S The function of heat- shock proteins ... binding of Drosophila heat shock factor to arrays of a conserved bp unit Cell 1991;64(3):585–93 Kumar M, Busch W, Birke H, Kemmerling B, Nürnberger T, Schöffl F, et al Heat shock factors HsfB1 and

Ngày tải lên: 26/05/2020, 21:11

... of Hsp70s was induced and the function of Hsp70s was analyzed Furthermore, two databases named Rice Heat Shock Proteins (RiceHsps) and Rice Gene Expression Profile (RGEP), and one online tool named ... (6%) and 30 (8%), respectively, significantly higher than that of 1,000 repeats of randomized Hsp70 interactors (empirical p-value < 0.001) (Figure 6) Identification of the binding sites of Hsp70s ... Proteins that were annotated as heat shock proteins and contained the specific domains of heat shock proteins were downloaded from Uniprot database; Second step: Hsp candidates, whose expression

Ngày tải lên: 02/11/2022, 10:51

... Hendershot and A Blum 1994 The heat shock response and expression of heat shock proteins in wheat render diurnal heat stress and field conditions Aust J Plant Physiol., 21: 857-67 Nollen, E.A and R.I ... translational control of sigma 32, where both Hsp bind to the sigma factor, preventing the transcription of heat shock genes King and Ferenci (2005) reported divergent of sigma factor in E coli under ... strains of rhizobia expressed HSP's at 40 and 45oC respectively The results are postulated by Zahran (1994) that an increased synthesis of 14 heat shock proteins in heat- sensitive strains and of heat

Ngày tải lên: 04/11/2020, 22:21

... methylome and to evaluate the effect of epigenetic therapy and cisplatin on putative epigenetically regulated genes and their related pathways in PDXs Methods: Genome-wide analysis of the DNA ... show a marginal effect of cisplatin but a strong demethylation effect of DAC in clinically relevant PDX models Identification of novel epigenetically regulated genes and pathways in PDX tumors ... (2016)8:7 Page 10 of 15 Fig Identification of putative epigenetically regulated key genes and pathways related to ovarian cancer using PDX tumors a Systematic strategy to identify CpG sites of novel putative

Ngày tải lên: 04/12/2022, 10:31

... Journal of Medical Sciences 2017; 14(3): 284-293 doi: 10.7150/ijms.17861 Research Paper Upregulation of heat shock protein 70 and the differential protein expression induced by tumor necrosis factor- alpha ... Tumor necrosis factor alpha (TNFα) plays diverse roles in liver damage and hepatocarcinogenesis with its multipotent bioactivity However, the influence of TNFα on protein expression of hepatocellular ... algorithm was applied and protein identifications with a probability of >99% with or peptides in at least one of the samples were retained Proteins that contained similar peptides and could not be

Ngày tải lên: 15/01/2020, 17:08

... expression and activity by heatshock transcription factor (HSF1) J Biol Chem 2000, 275:24970–24976 19 Zuo J, Rungger D, Voellmy R: Multiple layers of regulation of human heat shock transcription factor ... NF-κB signaling pathway is inhibited by heat shock independently of active transcription factor HSF1 and increased levels of inducible heat shock proteins Genes Cells 2011, 16:1168–1175 22 Piddubnyak ... transcriptional activity of HSF1 is not necessary for increased expression of ABC genes, which is probably mediated by HSF1 regulatory domain Keywords: Heat shock transcription factor 1, Heat shock proteins,

Ngày tải lên: 05/11/2020, 05:25

... 5692 and 6101 up- and downregulated genes When combined with the nearest-neighbor genes of ER binding sites, 289 up -regulated genes and 198 downregulated genes were associated with the ER binding ... ER -regulated genes were found in the vicinity of ER binding sites Furthermore, we annotated the function of the nearest-neighbor genes of these binding sites using Gene Ontology (GO), KEGG, and ... these genes, 33 upregulated genes and 11 downregulated genes were also identified by published ChIP-PET analysis [27] Our analysis found that more binding sites were associated with ER up-regulated

Ngày tải lên: 02/11/2022, 11:34

... Imam, Dipankar Maiti, N.P Mandal, Chandeshwar Prasadand Mukund Variar 2019 Identification of Broad Spectrum Blast Resistance Genes for North-East and Eastern India using Standard International Blast ... emergence of virulent forms of the pathogen by their frequent insertion into avirulence genes as reported in case of AvrPita, Avr-Pizt and Ace1 gene (Zhou et al., 2007; Li et al., 2009 and Bohnert ... population and selection of effective blast resistant genes or their combination for the management of this disease In our previous study on molecular diversity and mating type distribution of M oryzae

Ngày tải lên: 09/01/2020, 14:56

... America 97: 4392–4397 Hu, W., Hu, G., and Han, B 2009 Genome-wide survey and expression profiling of heat shock proteins and heat shock factors revealed overlapped and stress specific response under ... Mahanty, S., and Reddy, M.K 2016 Role of Heat Shock Proteins in Improving Heat Stress Tolerance in Crop Plants Heat Shock Proteins 283– 307 Sable, A., and Agarwal, S 2018 Plant Heat Shock Protein ... Chakraborty, S., and Chakraborty, N 2018 Heat Shock Proteins and Abiotic Stress Tolerance in Plants Heat Shock Proteins 41–69 Mittler, R., Finka, A., and Goloubinoff, P 2012 How plants feel the heat? Trends

Ngày tải lên: 14/01/2020, 10:49

... Chaoyang Zhang1,4 Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of Chinese Ministry of Education, Xiangya Hospital, ... cycle Figure Heat map of expression profiling of several key genes from the protein-protein interaction network analysis results Four genes were down -regulated and thirteen genes were up -regulated, ... replication-associated pathways Ten up -regulated DEGs and eight down -regulated DEGs were significantly enriched in p53 signalling pathways Eleven down -regulated DEGs and 26 up -regulated DEGs were

Ngày tải lên: 15/01/2020, 10:52

... 32,759 unigenes from seed samples We then matched the unigenes to 187 pathways and identified 47 PUFA biosynthesis related genes We verified the expression levels of 11 randomly selected genes from ... mechanisms and regulatory pathways of polyunsaturated fatty acid biosynthesis in camelina The genes characterized in our research will provide candidate genes for the genetic modification of crops ... profile includes a low intake of both saturated and omega-6 fatty acids and a moderate intake of omega-3 fatty acids [2] However, the majority of vegetable oils contains excessive amounts of

Ngày tải lên: 26/05/2020, 21:12

... R Abane and V Mezger 113 Trinklein ND, Chen WC, Kingston RE & Myers RM (2004) Transcriptional regulation and binding of heat shock factor and heat shock factor to 32 human heat shock genes during ... Role of Heat Shock Factor in cerebral cortex formation and as a regulator of p35 expression Genes Dev 20, 836–847 124 Morrison AJ, Rush SJ & Brown IR (2000) Heat shock transcription factors and ... of modifier genes that would enhance or diminish the impact of HSF2 deficiency Pending questions for the roles of HSF1 and HSF2 in oogenesis and in early embryos The role of HSF2 in oogenesis and...

Ngày tải lên: 18/02/2014, 04:20

... in vaccinia virus infected human and heat shock RT-PCR1analysis of the heat shock factor blood macrophages RT-PCR analysis of the heat shock factor and heat shock proteins in vaccinia virus infected ... after h of virus adsorption and fresh medium added Induction of the heat shock protein synthesis requires earlier activation of heat shock factors HSF1 is assumed to be the main mediator of the ... Differential expression and regulation of hsp70 and hsp90 by phorbol esters and heat shock J Biol Chem 1995, 270:14094-14099 Victor M, Benecke BJ: Expression levels of heat shock factors are not functionally...

Ngày tải lên: 11/08/2014, 08:21

... -test and statistical inferences of gene changes Bioinformatics 2001, 17:509-519 Fernandes M, Xiao H, Lis JT: Binding of heat shock factor to and transcriptional activation of heat shock genes ... 40 cycles of 94°C, 60 sec; 60°C, 30 sec and 72°C, 60 sec The primer pairs to amplify heat- shock element and 3' ends of the genes for heat- shock proteins 26 (3' UTR) and 70 (5' HSE and 3' UTR) ... derived from heat- shocked and non -heat- shocked embryos [37] Of the genes represented on the custom array, 21 are found in our top 188 Hsf-binding genes; of these, seven genes (Hsp26, 27 and 23, DnaJ-1,...

Ngày tải lên: 14/08/2014, 14:21

... expression of more than 50 genes, out of 7,075 genes that were examined [11] Exposure of the human T-cell line CCRF-CEM to cadmium altered the mRNA levels of more than 100 genes in a dose- and time-dependent ... understanding of the global transcription profile, current C elegans GO data are not sufficient to predict the functions of all of the cadmium-responsive genes Several C elegans cadmium -regulated genes ... of other interactions Late response Down -regulated Figure Heat map of cadmium-responsive genes Heat map of cadmium-responsive genes Cadmium responsive genes (≥2fold) based on decreased expression...

Ngày tải lên: 14/08/2014, 07:21

... cycle of 15 at 95 °C, amplification cycles of 0.5 at 94 °C, 0.5 at 50 °C and 0.5 at 72 °C, and one termination cycle of at 72 °C, with 35 cycles in total for HSF1a and HSF1b and 30 for heat shock ... Morimoto, R.I (1998) Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators Genes Dev 12, 3788–3796 Pirkkala, ... Regulation of heat shock factor trimer formation: role of a conserved leucine zipper Science 259, 230–234 19 Zuo, J., Rungger, D & Voellmy, R (1995) Multiple layers of regulation of human heat shock...

Ngày tải lên: 19/02/2014, 12:20

... Differences between heat and cold responses could arise from differential activation of the various Drosophila heat shock factor (HSF) isoforms [54] or from incomplete activation of HSFs under cold ... expression of Hsp67 is upregulated by heat stress [32,33] Therefore, it seems that, unlike the other Hsp genes tested here, Hsp67 does not respond similarly to heat and cold stress The absence of transcriptional ... expression changes in the same set of heat shock genes However, differences exist between the two responses: the level of expression is much higher for heat stress, and the response is also direct,...

Ngày tải lên: 06/03/2014, 09:22

... regulating the expression of heatinducible genes other than classical heat shock genes Expression of nonclassical heat shock genes induced by HSFs Although the heat shock response was originally ... half of the target genes are expressed during heat shock [59] We now refer to these heat- inducible genes that are different from the classical heat shock genes as ‘nonclassical heat shock genes ... expression of nonclassical heat shock genes was induced by mouse HSF3 or chicken HSF1, nonclassical heat shock genes were identified in MEF cells [31] Induction of one of the nonclassical heat shock genes, ...

Ngày tải lên: 18/02/2014, 04:20

... regulation and binding of heat shock factor and heat shock factor to 32 human heat shock genes during thermal stress and differentiation Cell Stress Chaperones 9, 21–28 Alastalo TP, Hellesuo M, Sandqvist ... (1998) Direct sensing of heat and oxidation by Drosophila heat shock transcription factor Mol Cell 2, 101–108 Ahn SG & Thiele DJ (2003) Redox regulation of mammalian heat shock factor is essential ... an activator and a repressor of heat shock genes by alternative splicing J Biol Chem 274, 27845–27856 Hu Y & Mivechi NF (2006) Association and regulation of heat shock transcription factor 4b with...

Ngày tải lên: 18/02/2014, 04:20

... Roles of heat shock factors in gametogenesis and development FEBS J 277, 4150–4172 Pirkkala L, Nykanen P & Sistonen L (2001) Roles of the heat shock transcription factors in regulation of the ... (1998) Direct sensing of heat and oxidation by Drosophila heat shock transcription factor Mol Cell 2, 101–108 Ahn SG & Thiele DJ (2003) Redox regulation of mammalian heat shock factor is essential ... SWI ⁄ SNF complex for robust nucleosome displacement at promoters of heat shock factor and Msn2- and Msn4 -regulated heat shock genes Mol Cell Biol 28, 1207–1217 57 Erkina TY, Zou Y, Freeling S,...

Ngày tải lên: 18/02/2014, 04:20