chaperones heat shock proteins as anticancer vaccines

... impaired clearance of oxidized proteins [35] Heat shock or stress proteins (Hsps) are expressed in neurons of polyQ diseased brains and have recently been identified as potent inhibitors of polyQ ... Rubinsztein DC (2000) Effects of heat shock, heat shock protein 40 (HDJ-2), and proteasome inhibition on protein aggregation in cellular models of Huntington’s disease Proc Natl Acad Sci USA 97, ... Macklis RM, Chisolm GM & Almasan A (2003) The late increase in intracellular free radical oxygen species during apoptosis is associated with cytochrome c release, caspase activation, and mitochondrial...

Ngày tải lên: 19/02/2014, 06:20

... a-crystallin-related small heat shock proteins: HspB1–10 Cell Stress Chaperones 8, 53–61 2622 Y Sun and T H MacRae 23 Taylor RP & Benjamin IV (2005) Small heat shock proteins: a new classification scheme ... expression of the small heat 2625 Small heat shock proteins and disease 120 121 122 123 124 125 126 127 128 shock proteins Hsp27 and [alpha]B-crystallin in Parkinson’s disease Neuroreport 10, 2273–2276 ... sHSP associated diseases are summarized in Table FEBS Journal 272 (2005) 2613–2627 ª 2005 FEBS Small heat shock proteins and disease Therapeutic implications of sHSPs and other molecular chaperones...

Ngày tải lên: 19/02/2014, 18:20

... monomer in the case of TaHsp17.8C-II was added to 0.3 lM MDH as a negative control Aggregation protection of firefly luciferase (Luc) (Promega) was assessed as follows Luc at lM was heated with 12 ... was performed as above except the mobile phase was 200 mM Na/Pi, 100 mM NaCl, pH 7.3 Firefly luciferase reactivation assays Luc was heat- inactivated at 42 °C in the presence of sHsp as described ... different substrates, class II proteins have proven to be less effective as chaperones than class I proteins, consistent with some type of substrate specificity for these two classes of proteins At the...

Ngày tải lên: 07/03/2014, 15:20

... oligomerization of class B proteins A temperature-controlled shift towards smaller species, similar to that found with class A proteins, was observed with HspF, a class B sHsp (Fig 2A) The protein was previously ... iodide, and processed using MASSLYNX software (Micromass UK Ltd) The peak series were identified manually A mass was generated based on an algorithm built into the MASSLYNX software, where m/z-1 ... small heat- shock protein Nature 394, 595–599 van Montfort, R.L., Basha, E., Friedrich, K.L., Slingsby, C & Vierling, E (2001) Crystal structure and assembly of a eukaryotic small heat shock protein...

Ngày tải lên: 23/03/2014, 12:20

... Small heat shock proteins are molecular chaperones J Biol Chem 268, 1517–1520 19 Yang, H., Huang, S., Dai, H., Gong, Y., Zheng, C & Chang, Z (1999) The Mycobacterium tuberculosis small heat shock ... 40 41 42 43 heat- shock protein alphaB-crystallin J Biol Chem 274, 30190– 30195 Clark, J.I & Muchowski, P.J (2000) Small heat- shock proteins and their potential role in human disease Curr Opin ... viability by h, decreased dramatically in cells containing the pET 16b control vector as measured by the CFUs In contrast, the viability of cells that over-expressed MTB HSP 16.3 decreased by two orders...

Ngày tải lên: 23/03/2014, 21:21

... five class A (HspA, HspB, HspD, HspE and HspH) and two class B proteins (HspC and HspF), as well as the class A E coli proteins IbpA and IbpB A representative example Ó FEBS 2002 from each class, ... As demonstrated previously [13], full-length class A and class B a-Hsp proteins of B japonicum act as efficient chaperones in vitro (Fig 2H,I) They form oligomers with an apparent molecular mass ... (1994) Expression and Function of the Low-molecular-weight Heat Shock Proteins In The Biology of Heat Shock Proteins and Molecular Chaperones (Morimoto, R.I., ` Tissieres, A & Georgopoulos, C.,...

Ngày tải lên: 31/03/2014, 21:21

... host heatshock proteins in the malignant transformation of a chronically infected host has been shown to be integral This review attempts to assemble the evidence implicating heat- shock proteins ... 8:12 Srivastava PK, Udono H, Blachere NE, Li Z: Heat shock proteins transfer peptides during antigen processing and CTL priming Immunogenetics 1994, 39:93-98 Srivastava P: Heat shock proteins ... hsf1 in particular has been found to be overexpressed and to contribute to invasion and metastasis by permitting increased cell proliferation and by decreasing cell death [44-47] As Page of 10 (page...

Ngày tải lên: 10/08/2014, 22:20

... Motivation Neurodegeneration has been considered as a main cause for different types of neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, dementia, and stroke [1,2] ... 107, 09B322 (2010) Asahi Tomitaka, Hiroki Kobayashi, Tsutomu Yamada, Minhong Jeun, Seongtae Bae, and Yasushi Takemura, “Magnetization and Self-Heating Temperature of NiFe2O4 Measured by Applying ... MMM-Intermag Conference, Washington, DC, USA (2010, 1, 18 ~ 22) Asahi Tomitaka, Hiroki Kobayashi, Tsutomu Yamada, Minhong Jeun, Seongtae Bae, Yasushi Takemura, “Magnetization and Self-Heating Temperature...

Ngày tải lên: 09/09/2015, 10:08

... INTRODUCTION 13 It has been 42 years since the heat shock response was first reported in a Drosophila study [Ritossa, 1962] The heat shock proteins (HSPs) are highly conserved proteins that are ... recombinant heat shock proteins in the presence or absence of polymyxin B for another 16 h The NF-κB-Luciferase expression was determined using the Dual Luciferase Reporter Assay (DLR Assay, Promega) ... expression 28 Measurement of cytosolic calcium 28 In vitro stimulation assay 29 Toll-like receptor (TLR4) activation assay 29 RESULTS 31 Purification of recombinant heat shock proteins under 31...

Ngày tải lên: 09/10/2015, 11:06

... also operate in the present case The heat- induced activation of kinases such as Akt has been shown to increase HSF1 activity Enhanced Ras maturation by heat stress was associated with a heightened ... phospholipase C (PLC) [25] The costimulation of phospholipases such as PLC and PLA2 by heat shock and the resultant release of lipid mediators could also enhance the subsequent membrane association ... mitogenactivated protein kinase pathways by heat shock Cell Stress Chaperones 7, 200–208 Membrane fluidity and heat shock response Gabai VL & Sherman MY (2002) Interplay between molecular chaperones and signaling...

Ngày tải lên: 07/03/2014, 12:20

... such as tumor necrosis factor and Fas by binding of their respective ligands and the subsequent recruitment of caspase-8 [44] Caspase-8 can activate effector caspases, such as caspase-3 and caspase-7, ... the active forms of caspase-3 and PARP cleavage by treatment with H2O2 In contrast, although caspase-3 was markedly activated after heat shock, activation of caspase-3 was not affected by overexpression ... procaspase-9 was processed to active forms (37 and 35 kDa fragments), and the activation of caspase-9 was suppressed by overexpression of Hsp105a or Hsp105b (Fig 4A) In contrast, caspase-9 was...

Ngày tải lên: 16/03/2014, 06:20

... as a response to caspase activation as documented by the Barros Okada group22 With increasing duration of light exposure of HeLa cells expressing the KRED-Lamin B1 protein, the cell number was ... taken 24 h later directly, without washing, to demonstrate intracellular localization and distribution of the fluorescent proteins and fusion proteins (FPs) as well as the apoptotic and dead cells ... protein tyrosine phosphatases (PTPs) are considered as excellent examples, as illustrated in Figure The consequences of oxygen activation in human bodies are indeed increasingly observed but only...

Ngày tải lên: 26/10/2012, 09:48

... activator of caspases), activation of caspase-3 and caspase-9, and apoptosis in C2C12 myogenic cells [24] Stankiewicz et al [25] found that Hsp70 overexpression stabilizes Mcl-1 protein in heat- shocked ... Second, the activity of caspase-3 was analysed as a marker of apoptotic cells using an in vitro substrate-cleaving reaction As shown in Fig 6D, H2O2-induced apoptosis was increased in cells transfected ... of caspase-3 using the caspase colorimetric assay kit Data were obtained from four independent experiments *P < 0.05 versus control group; #P < 0.05 versus pcDNA3.1 + H2O2 group (D) Caspase-3...

Ngày tải lên: 16/02/2014, 09:20

... expression of heatinducible genes other than classical heat shock genes Expression of nonclassical heat shock genes induced by HSFs Although the heat shock response was originally characterized based ... nonclassical heat shock genes as well as classical heat shock genes play roles in ameliorating disease progression One therapeutic strategy for protein-misfolding disorders such as polyQ disease ... from heat shock and in maintaining protein homeostasis without inducing the expression of the classical heat shock genes It was revealed recently that there are numerous nonclassical heat shock...

Ngày tải lên: 18/02/2014, 04:20

... (2010) A novel mouse HSF3 has the potential to activate nonclassical heat- shock genes during heat shock Mol Biol Cell 21, 106–116 Sorger PK & Pelham HR (1988) Yeast heat shock factor is an essential ... L (2001) Roles of ¨ the heat shock transcription factors in regulation of the heat shock response and beyond FASEB J 15, 1118–1131 Fujimoto M & Nakai A (2010) The heat shock factor family and ... to the heat shock promoter by HSF upon heat shock Mol Cell 8, 9–19 72 Hong S, Kim SH, Heo MA, Choi YH, Park MJ, Yoo MA, Kim HD, Kang HS & Cheong J (2004) Coactivator ASC-2 mediates heat shock...

Ngày tải lên: 18/02/2014, 04:20

... HSF3 has the potential to activate non-classical heat shock genes during heat shock Mol Biol Cell 21, 106–116 Trinklein ND, Murray JI, Hartman SJ, Botstein D & Myers RM (2004) The role of heat shock ... activation by yeast heat shock transcription factor through two different types of heat shock elements J Biol Chem 282, 10333–10340 Sakurai H & Takemori Y (2007) Interaction between heat shock transcription ... mouse as an orthologue of the chicken HSF3 gene; however, the human HSF3 gene is a pseudogene [41] Mouse HSF3 translocates into the nucleus upon heat shock and may activate nonclassical heat shock...

Ngày tải lên: 18/02/2014, 04:20

... HSF1 binding via chromatin remodelling Heat shock genes are not HSF4 targets, but HSF4 regulates a set of nonclassical heat shock genes in response to heat shock in the lens This semicomprehensive ... Transcriptional regulation and binding of heat shock factor and heat shock factor to 32 human heat shock genes during thermal stress and differentiation Cell Stress Chaperones 9, 21–28 114 Yamamoto N, ... (1994) Expression of heat shock factor in mouse testis: potential role as a regulator of heatshock protein gene expression during spermatogenesis Biol Reprod 50, 1334–1343 68 Alastalo TP, Lonnstrom...

Ngày tải lên: 18/02/2014, 04:20

... conditions, such as heat shock, mRNA translation undergoes rapid changes In response to heat shock stress, cells induce the expression of a unique set of proteins called heat shock proteins (HSPs) ... however, was a little higher than what was expected from the reduced abundance of both polypeptides in heat- shocked cells During the recovery period from heat shock, as compared to the non -heat- shocked ... from heat shock appeared to be similar to what was observed in the exponentially growing cells PABP expression during heat shock recovery Association between PABP1 and eIF4G following heat shock...

Ngày tải lên: 18/02/2014, 12:20

... after and days were significantly decreased (Fig 10F,H) Discussion Heat shock proteins (HPSs) have very important functions, such as acting as molecular chaperones under physiological conditions ... of small heat shock proteins, which protect against apoptotic cell death triggered by various stimuli such as oxidative stress, and increase the antioxidant defense of cells by decreasing the ... LG (2001) Unfolding the role of chaperones and chaperonins in human disease Trends Genet 17, 528–535 Takayama S, Reed JC & Homma S (2003) Heat- shock proteins as regulators of apoptosis Oncogene...

Ngày tải lên: 18/02/2014, 17:20

... library was constructed from RTG-2 cells by using a SuperScript Plasmid System for cDNA Synthesis and Plasmid Cloning kit (Invitrogen) and was used as the PCR template For 5¢-RACE, the first PCR was ... Georgopoulos, C (1994) The Biology of Heat Shock Proteins and Molecular Chaperones Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York Wu, C (1995) Heat shock transcription factors: structure ... zebrafish (Danio rerio) heat shock factor mRNAs in response to heat stress J Exp Biol 203, 1817–1824 ˚ Airaksinen, S., Rabergh, C.M.I., Sistonen, L & Nikinmaa, M (1998) Effects of heat shock and hypoxia...

Ngày tải lên: 19/02/2014, 12:20