Translatomics study of chinese hamster ovary cell cultures

... Batch cultures 49 3.1.4 Feeding of batch cultures 49 3.1.5 Rapamycin treatment of batch cultures 50 3.1.6 Determination of cell viability 50 3.1.7 Determination of ... Background Chinese hamster ovary (CHO) cells are one of the most commonly used mammalian host cell lines by biopharmaceutical industries for the production of recombinant proteins of therapeutic ....

Ngày tải lên: 08/09/2015, 19:49

... noisnetxe lanif a neht dna ,ces 03 nim rof Co27 ta noisnetxe ,ces 03 rof Co06 ta gnilaenna ,ces 03 rof Co49 ta noitarutaned fo selcyc 03 yb dewollof ,nim rof Co49 ta noitarutaned laitini na fo ... slleC noitatipicerponummI enivob fo noitpircsnart eneg rof neercs ot evoba debircsed sa demrofrep neht saw RCP dna ,ANR evomer ot nim 02 rof Co73 ta H esaNR fo stinu htiw detaert erew ANDc dednarts-...

Ngày tải lên: 07/08/2014, 20:23

... modifications on heparin binding, recombinant CHO cells were treated with inhibitors of tyrosine sulfation and N-glycosylation under conditions that allowed partial inhibition of these modifications ... In summary, we present evidence for a very strong similarity of HCII from human serum and its recombinant counterpart from CHO cells with respect to tyrosine...

Ngày tải lên: 08/03/2014, 22:20

... chuyển gen v protein tái tổ hợp tiến hnh hớng nghiên cứu nuôi cấy dòng tế bo CHO- K1 để chuyển gene, sau chọn lọc quần thể tế bo CHOK1 sản xuất EPO ổn định Tế bo CHO- K1 đợc chọn lm tế bo chủ chúng ... - Tế bo CHO- K1 (ATCC CCL 61) - Hóa chất dùng cho chuyển gene ExGen 500, PCR, điện di, ELISA hãng Sigma, Merck, Fermentas, Invitrogen đợc pha tùy theo...

Ngày tải lên: 28/08/2013, 14:15

... Table lists details on the CTB4 Corpus data used in this study Num of Files Num of Sentences Num of Words Num of Phrases Table 1: Definition of Chunks 2.2 Data Representation Training 728 9,878 238,906 ... Conference on Machine Learning (ICML01) In this paper, we conducted an empirical study of Chinese chunking We compared the performance of four models, SVMs, CRFs, MBL,...

Ngày tải lên: 08/03/2014, 02:21

... caspase-3 is involved in OA-induced DNA fragmentation independently from DEVDase activity, and that DEVDase or DEVDase-like activity is involved in DNA fragmenta- Involvement of caspase-3 in DNA fragmentation ... cells In the case of caspase-3) ⁄ ) cells, DEVDase or DEVDase-like activity may play an important role in inducing DNA fragmentation OA induces caspase...

Ngày tải lên: 22/03/2014, 21:20

... Y Nδ Nδ Y Y Nγ Nγ Y Y Y Nγ Y Nγ Nγ Y Y Y Y Y Y Y Y Y Y Y Y LAEC Y Y Y Nα Y Nγ Nγ Y Y Y Nγ Y Nγ Y Y Y Y Y Y Y Y Y Y Y Y Y SAEC Nγ Y Y Y Nδ Nδ Y Y Y Y Y Nβ Y Y Y Y Y Y - LAEC Nγ Y Y Y Y Y Y Y Y ... Y Y Y Y Y Y Y Y Nβ Y Y Nγ Y Y Y - SAEC Y Y Y Nδ Y Y Y Y Nγ Y - LAEC Y Y Y Y Y Y Y Y Nγ Y - SA...

Ngày tải lên: 12/08/2014, 14:20

... (McLelland, 200 0a) Amateur manga, a new form of manga circulation, played a crucial role in the dissemination of BL manga Amateur manga is a manga produced, printed and distributed by artists themselves ... have the same level of acceptance with lesbian 28 manga, it may imply that portraits of male homosexuality and female homosexuality in Japanese manga...

Ngày tải lên: 24/08/2014, 12:17

... biochemically similar to human forms for increased product efficacy and safety (Matasci et al 2009) 2.3.1 Chinese hamster ovary cells Chinese hamster ovary (CHO) cells were first isolated in 1958 ... Mammalian cells like the Chinese hamster ovary (CHO) cells are commonly used for their ability to perform the required protein modifications for product safety and...

Ngày tải lên: 09/09/2015, 11:30

... Manifestations in Singapore and Malaysia”, in Sino-Malaysiana: Selected Papers on Ming & Qing History and on the Overseas Chinese in Southeast Asia 19 42- 1988 (Singapore: South 34 经》，说明琼瑶仙教的创教源起、信奉的神明和该教济世救民的方法与仪式。 ... 余淑娟〈戏曲赞助与街戏的变迁：以新加坡韭菜芭城隍庆典为例〉，见《民俗曲艺》 第155期 20 07）， 22 7 -26 4。 18 附录1.5.：重修告竣立...

Ngày tải lên: 11/09/2015, 09:57

... 林庆熙〈漫谈福建莆仙戏《目连》〉，见《福建目连戏研究文集》（福州：福建省艺 术研究所，1991）， 33 。 陈翘〈莆仙戏《目连》〉，见《福建目连戏研究文集》（福州：福建省艺术研究所， 1991），页127。 叶明生《莆仙戏剧文化生态研究》（厦门：厦门大学出版社，2007）， 36 9 -37 3。 口述历史访谈：2005 3 18日，访问对象：黄亚松，地点：黄氏Binchang Rise住 宅。九鲤洞的目连戏戏簿，一说是“得月楼”留下的。容世诚、张...

Ngày tải lên: 11/09/2015, 09:57

... 应靖元音法坛的陈义涛前來主持道教法会。历年的经筵主事如下表所示： 年份 经筵 资料来源 1 944 恒清坛卢大和（万山街13号） 戴文荣口述 19 54 郑明源等本地经师 九鲤洞第二届逢甲大普度纪念碑 19 64 郑明源等本地经师 黄亚松口述 19 74 马来西亚居銮珠光书院13 黄亚松口述 19 84 马来西亚居銮珠光书院 黄亚松口述 19 94 中国莆田三江口玄应靖郑玉春道长 九鲤洞第六届逢甲大普度资料 20 04 中国莆田兴化府玄应靖元音法...

Ngày tải lên: 11/09/2015, 09:57

... River 或 Sungei Kallang）的上游，从贝雅士蓄水池下池 （Lower Pierce Reservior）开始，朝东南流经大巴窑（Toa Payoh），再从加冷盆 地（Kallang Basin）出口，流向滨海湾（Marina Bay），通向大海。据说这些鬼 路灯就是透过这条水道，从世界各地水路而来的诸鬼带到九鲤洞。 逢甲大普度期间，幡灯内需点上油灯，晚间得时时添油，不使灯灭。为 ... 铃作响，似乎有昭示诸鬼的用意。幡竿下得摆设油灯一盏和香炉一个。 162 图 2...

Ngày tải lên: 11/09/2015, 09:57

... 道、人道和天道的众生。论文提出中国宗教仪式剧是一种以“看不见的观众” 176 （invisible audience）为主要表演对象的戏剧演出。这一“看不见的观众”包含 了天道、地狱道、饿鬼道的众生，进而解释了中国宗教仪式剧为甚么经常没有 或是很少出现人类的观众，以及中国宗教仪式剧中具有许多禁忌的原因。 177  ... 影戏、歌舞小戏等各种“民间戏剧”，皆应排除在“中国戏剧”这一概 邱坤良《民间戏曲散记》（台北：时报文化出版社，1 97...

Ngày tải lên: 11/09/2015, 09:57