growth kinetics of cell cultures

... rate of incorporation of Si from the liquid phase to the lattice The present results suggest that Pt is a good candidate for the VLS growth of Si nanowires However, the growth temperature of the ... Nevertheless, the results illustrate some fundamental aspects of growth kinetics of Si nanowire with Pt The development of a CMOS compatible Si nanowire growth process using Pt as a VLS catalyst is potentially ... the growth rate with 200 Length (µm) 150 100 50 0 10 15 Time (min) Fig Length of Si nanowires using Au and Pt versus time at holding temperature of 1000 °C Fig Construction of log-scaled growth...

Ngày tải lên: 16/03/2014, 15:05

... diffusional resistance of substrate into the biofilm due to the growth of the outer heterotrophic layer over the slower growing nitrifers During Phase 2, the diameter of biofilm particles increased ... Fig Maximum specific growth of heterotrophs Phase 5.00 40 Days of Operation 0 20 40 60 80 Days of Operation Fig Half saturation concentration Ks of heterotrophs Mass fraction of the microorganism ... density of particle decreased from 153 to 97 g/L Heterotrophic bacteria growth had contributed to a less dense biofilm during Phase According to Tijhuis et al (1995), biofilm density of the heterotrophs...

Ngày tải lên: 05/09/2013, 08:40

... deletion of the mnt gene, which encodes a member of the Mad family of antagonists of Myc, restores proliferation to c-myc deleted cells, suggesting that the balance of expression of members of the ... neuronal precursor cells leads to a dramatic loss of proliferative capacity of such cells (Knoepfler et al 2002) As long as questions of redundancy between different members of the myc gene family ... downstream of Myc may not exist, there can be little doubt that stimulation of protein translation and biogenesis is a major effector pathway of Myc Undoubtedly, the stimulation of cell growth will...

Ngày tải lên: 25/10/2013, 21:20

... Influence of Pi starvation on growth and secreted AP activity of tomato suspension cells Tomato cells cultured for days in the absence of exogenous Pi had only approximately 40% of the fresh weight of ... 8-day-old +Pi cells (approximately 63 and 25 g of cells were obtained per 500 mL culture of 8-day-old +Pi and –Pi cells, respectively) Eight days following subculture of the tomato cells into –Pi ... concentration of enzyme assayed One unit (U) of activity is defined as the amount of AP resulting in the hydrolysis of lmol of substrate per at 25 °C Phosphatase assay A For routine measurements of AP...

Ngày tải lên: 23/03/2014, 21:20

... Characterization of the cell types and growth kinetics of cultured cells Healthy growing EC and SMC cultures were obtained from UC of group-1 and group-2 individuals No contamination of SMCs in EC cultures ... 0.005) Several growth parameters of the different cell cultures were analyzed Figure shows the cell proliferation kinetics of the cell types obtained from individuals Cell culture growth follows ... weight and growth characteristics of cultured cells No differences in terms of average time to reach passage cell density, percentage of viable cells and senescence level were found for each cell culture...

Ngày tải lên: 18/06/2014, 15:20

... outgrowth assays that evaluate the ability of a fixed input of T cells to inhibit long-term growth of different numbers of target cells, without the addition of cytokines This experimental design ... of their active proliferation Figure Growth kinetics of CD4+ and CD8+ T cell lines The extrapolated mean total cell counts of CD4+ (left) and CD8+ (right) T cell lines cultured with IL-2, IL-7, ... outgrowth in 50% of replicate wells Results Analysis of in vitro growth kinetics To dissect the impact of different g-chain cytokines on human T cell in vitro expansion, we took advantage of a well...

Ngày tải lên: 18/06/2014, 16:20

... concentration of viral pro- Figure of hypertonic medium Kinetics intracellular RSV proteins in cells incubated in Kinetics of intracellular RSV proteins in cells incubated in hypertonic medium HEp-2 cells ... and re-appearance of intracellular viral pro- Figure Confocal laser scanning image of intracellular RSV proteins Confocal laser scanning image of intracellular RSV proteins HEp-2 cells had been ... viral glycConfocal laser scanning image of the distribution of viral glycoproteins on cell surface of epithelial cells HEp-2 cells had been infected at m.o.i of 50 for 12 h then incubated with anti-RSV...

Ngày tải lên: 18/06/2014, 18:20

... concentration of viral pro- Figure of hypertonic medium Kinetics intracellular RSV proteins in cells incubated in Kinetics of intracellular RSV proteins in cells incubated in hypertonic medium HEp-2 cells ... and re-appearance of intracellular viral pro- Figure Confocal laser scanning image of intracellular RSV proteins Confocal laser scanning image of intracellular RSV proteins HEp-2 cells had been ... viral glycConfocal laser scanning image of the distribution of viral glycoproteins on cell surface of epithelial cells HEp-2 cells had been infected at m.o.i of 50 for 12 h then incubated with anti-RSV...

Ngày tải lên: 20/06/2014, 01:20

... Page of case of Si and to the value of 1.5 × 1014 cm-2 s-1 in the case of Ge, to obtain the same velocity of growth of the planar films, set at a constant value of 0.05 nm s-1 The evaporated fluence ... 2D growth rates A comprehensive comparison of the axial growth rate in the case of Si and Ge NWs synthesized by EBE is shown in Figure This figure reports the increment of the fluence ΔF of both ... picture of the Si NWs (left-hand side) and of the Ge NWs growth on Si substrate (right-hand side), in different fluence regimes Color scale refers to the evolution of the growth as a function of the...

Ngày tải lên: 21/06/2014, 05:20

... metabolome of Saccharomyces cerevisiae In this whole -cell analysis, flux equates to growth rate Cell growth (the increase in cell mass through macromolecular synthesis) requires the synthesis of cellular ... regulation of cell growth [24,64,65] (see also the section on translational control, below) As an example, many genes reported to be involved in control of cell size or coordination between cell growth ... Loewith R, Hall MN: TOR signalling in yeast: Temporal and spatial control of cell growth In Cell Growth: Control of Cell Size Monograph 42 Edited by Hall MN, Raff M, Thomas G New York: Cold Spring...

Ngày tải lên: 06/08/2014, 18:21

... contents of free (F), soluble ester-bound (SE), cell wall ester-bound (SWE) and soluble glycoside-bound (SG) phenolic acids in control cells of embryogenic cultures of Norway spruce (C) and in the cells ... 8-h level, but levels of the other forms of phenolic acids increased; levels of F by 70% and levels of CWE by 25% (Fig 1) After 24 h of WF treatment a decline in the contents of total phenolics ... doubled content of F after 12 h were observed in WF-elicited cells (Fig 1) In PF-elicited cells, the level of SG increased by about 40% after h After 12  h of treatment with PF the amount of SG was...

Ngày tải lên: 07/08/2014, 10:21

... activation of apoptosis and inhibition of cell growth in embryonic tbx5 morphants at 36 hpf by transcriptional and translational levels By RTPCR, the members of BCL-2 family genes and cell cycle-related ... to the end sequel of apoptosis and cease of cell growth Besides, the distribution of aberrant apoptosis and deviant cell cycle caused by a deficiency of tbx5 in the embryos might be associated ... resulted in activation of apoptosis and inhibition of cell growth in whole individual of zebrafish embryo, though knockdown of TBX5 leads to overexpression of both anti-apoptotic and pro-apoptotic gene...

Ngày tải lên: 10/08/2014, 10:20

... decreased the number of cells only in P1-F344-cells, but not in P2-F344- and P2-Lewis-cells Proportion of SA-b-gal-positive cells did not correlate with cell number, as this amount of cells was not ... whole cell body in Lewis cells (Figure 1d) Effects of a-amylase on cell growth in cells from F344 and Lewis rats It has not yet been described, if a-amylase has effects on mammary gland cell growth ... http://www.jeccr.com/content/30/1/102 U/ml Page of 12 50 U/ml a) Total number of cells b) Viable cells c) Dead cells Figure Change in cell number after treatment of F344 and Lewis cells with salivary a-amylase...

Ngày tải lên: 10/08/2014, 10:21

... effects on the growth rates of the low arginase producer cell lines CL-2 (P = 0.14) and Renca (P = 0.07) Cell viability of the cells was > 95% at the different points and conditions of the experiments ... possible the growth of renal cell carcinoma cells is arginase II independent resulting in the low inhibitory effect of nor-NOHA Page of 10 (page number not for citation purposes) Journal of Hematology ... after 48 hours of coculture of Jurkat cells with the cell lines Levels of L-arginine were significantly lower (* P < 0.001) in CL-19 co -cultures than in Jurkat control or in co -cultures with CL-2...

Ngày tải lên: 10/08/2014, 22:20

... method involving rinsing of cell cultures, mainly measured firmly attached cell attachment and viability These results emphasize the importance of consideration of the type of assay utilized in assessing ... cells [29] The detachment of Caco-2 cells from cell culture substrates is therefore possibly due to the onset of apoptosis Lopez et al reported that in the presence of serum, concentrations of ... be a high enough amount of the Cd2+ to cause acute cell death in Caco-2 cells However, the release of a small amount of Cd2+ into the cell culture medium from QDs Page of 15 (page number not for...

Ngày tải lên: 11/08/2014, 00:22

... first, upper semicell after the second cell division (E) Cell line 18 Upper semicell resulting from the first cell division, after which the cell died (F-I) Cell line 13 Loss of growth polarity ... shedding of the primary cell wall [41] Genes involved in cell wall metabolism Phylogenetic relationship of M denticulata expansinresembling proteins As the involvement of expansins in cell growth of ... (C-E) Elongation growth is reduced, lateral lobes are fused (C) Cell line Lower semicell formed after the second, upper semicell after the third cell division (D) Cell line Lower semicell formed after...

Ngày tải lên: 11/08/2014, 11:21

... A B Figure RT-PCR 2of total RNA extracted from hRz-C6/36 cells RT-PCR of total RNA extracted from hRz-C6/36 cells (A) hRz expression in the cells was detected by the presence of an hRz-specific ... quantitation of DENV titer in cellular medium To evaluate the impact of hRzs on assembly and release of DENV, we performed qRT-PCR on the viral RNA extracted from cell supernatant of the infected ... level of specificity of the ribozyme In this study, the length of each arm ranges from 5–10 bp The sequences of hRz templates, positions of the triplets on DENV genome, and the specific strains of...

Ngày tải lên: 12/08/2014, 04:21

... understanding of cellular responses to environmental change Reports of metabolite profiling in the context of defence-related plant secondary metabolism, although rare, include the analysis of elicitor-treated ... the lack of a comprehensive spectral library of standard compounds In this paper, we report the application of 1H NMR to characterize the metabolome of elicitor-induced opium poppy cell cultures ... Biology 2008, 8:5 Results Global metabolite profiling of the elicitation response Aqueous extracts of control and elicitor-treated cell suspension cultures of opium poppy were analyzed in D2O by 1H...

Ngày tải lên: 12/08/2014, 05:20

... of small airway epithelial cells Lineage Lineage verification of small airway epithelial cells A & B; Gene expression of CCSP (CC-10) in small airway (SAEC) cell cultures, large airway cell cultures ... expression of Surfactant Protein-B (SP-B) in small airway (SAEC) cell cultures, large airway cell cultures (LAEC) at p0 and p2 as well as large airway epithelial cell line 16HBE14o- cells as compared ... the five failed cultures was 0.326 ± 0.055 × 106 cells, which is significantly lower than the cell yield for successful cultures (1.071 ± 0.070 × 106 cells; p < 0.01) The presence of BOS significantly...

Ngày tải lên: 12/08/2014, 14:20