IMMOBILIZATIONS OF ANTI JEV ANTIBODIES IN HUMAN SERUM FOR DIRECT IMMUNOSENSORS

... CLEANING PROCEDURES sensors 3-aminopropyl-triethoxy-silance (APTES) Experimetal immobilizations of anti JEV serum APTES – Antibody - Immobilization with mg/ml anti- JEV antibodies contained human serum ... solution for 30 - Immobilization with mg/ml anti- JEV antibodies contained human serum for hour -Blocked with 2% BSA solution in PBS for 30 minutes - Was...

Ngày tải lên: 15/04/2015, 09:20

... strongly to α-actinin [12,13] Moreover, an increase in serum anti-α-actinin antibodies in lupus patients was associated with a 2.5-fold increase in the prevalence of LN [14] Finally, levels of anti-α-actinin ... followed by Manson and colleagues [1] were being treated, and anti-α-actinin antibody titers may decrease with therapy Finally, perhaps anti-dsDNA antibodie...

Ngày tải lên: 12/08/2014, 11:22

... antibodies and TNX proteins were diluted in T-NaCl ⁄ Pi containing 0.1% BSA Multiple species of tenascin-X in human serum Binding of TE to serum TNX was performed as described above Briefly, serum ... species of tenascin-X in human serum minal species The failure of serum TNX to bind to collagen types I, III and V [18,20,21] is surprising because we...

Ngày tải lên: 23/03/2014, 09:21

... between anti-nucleophosmin and anti-cardiolipin antibodies in lupus mice A positive correlation between anti-nucleophosmin and anti-cardiolipin antibody reactivities in 38 sera from male WB mice ... Immunoblot analyses of sera positive for anti-nucleophosmin antibody (a,c) Anti-nucleophosmin antibody obtained respectively from a patient with antibody systemic...

Ngày tải lên: 09/08/2014, 07:20

... depletion of reduced glutathione (namely, gamma-glutamyl-Lcysteinyl-glycine [GSH]) by using ethacrynic acid or 1-pyrrolidinecarbodithioic acid specifically counters the inhibition of spontaneous ... required in each mitotic cycle if cell activation is maintained by a hit and run mechanism as previously proposed by Mitsuaki Yoshida in 1986 [343] A variety of other processes inv...

Ngày tải lên: 13/08/2014, 09:20

... evaluation of heme dynamics in cultured cells Role of heme metabolism in cellular heme content Regulatory role of free heme in expression of HO-1 To evaluate the contribution of heme synthesis and ... HO-1 protein was also induced by the treatment with HO-2 siRNA in HepG2 cells These results indicate that the down-regulation of HO-2 expression is...

Ngày tải lên: 19/02/2014, 05:20

... difficulties in understanding the functional role of receptor cleavage and shedding Some mutations or small deletions of individual or groups of amino acids did not prevent receptor cleavage, due ... a role for the extracellular domain in the stabilization of an inactive form of the unliganded receptor [29] Indeed, only cleavage in the E3 domain of the rec...

Ngày tải lên: 21/02/2014, 00:20

... endocannabinoid system, we investigated the presence and regulation of endocannabinoids, cannabinoid receptors and FAAH in immature and mature dendritic cells obtained by stimulation with either the ... min), two fractions were obtained: a top leukocyte band containing mononuclear cells (monocytes and lymphocytes) and a lower band containing polymorphonuclear le...

Ngày tải lên: 22/02/2014, 07:20

... fMLP alone (Fig 2B,C) with > 90% of the [3H]PtdBut accumulating within the first of stimulation Effect of PI3-kinase and ARF inhibitors on O2 À and PLD activation Selective pharmacological inhibitors ... significant inhibition of O2 À generation and [3H]PtdBut accumulation over untreated controls Inserts show the concentration effects of brefeldin A or wortmannin on O2...

Ngày tải lên: 07/03/2014, 15:20

... group of GHMP kinases in which proton transfer does not play a major role in the rate determining step of catalysis That five of the 10 disease-causing mutations resulted in insoluble protein in ... play a major role in the rate-determining step of catalysis Several of the disease-causing mutations are not soluble following induction in E coli Although all the mut...

Ngày tải lên: 08/03/2014, 02:20

... Wonders of the Human Body," and that quaint volume, "Odd Hours of a Physician," have aided the author with facts and fancies The writings of Draper, Dalton, Carpenter, Yalentin, Mapother, Watson, Lankester, ... because we can feel the pulsations of the brain through them, like the bubbling of water in a fountain They gradually diminish in size, owing to...

Ngày tải lên: 15/03/2014, 13:20

... than the activity in the precipitate from the Ca1 transfected cells demonstrating that the Cb2 antibody in combination with PKA kinase assay could be used to detect specifically Cb2 activity To ... PKA- specific kinase activity, as shown in Fig 4B This demonstrated that precipitates extracted with 8-CPTcAMP contained cAMP-inducible PKA activity that could be inhibited by protein kina...

Ngày tải lên: 16/03/2014, 18:20

... Fasano M (2007) Modulation of heme and myristate binding to human serum albumin by anti-HIV drugs An optical and NMR spectroscopic study FEBS J 274, 449 1–4 502 19 Fanali G, Ascenzi P & Fasano ... Fasano M, Amoresano A, Salzano AM, Pucci P et al (2002) Binding and relaxometric properties of heme complexes with cyanogen bromide fragments of human serum al...

Ngày tải lên: 23/03/2014, 04:21

... Timson Disease-causing mutations in human GALE Fig Crosslinking of wild-type and disease-causing mutants of UDP-galactose 4-epimerase (GALE) Crosslinking was carried out with N-(3dimethylaminopropyl)-N¢-ethylcarbodiimide ... during purification Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the nine disease-causing m...

Ngày tải lên: 23/03/2014, 11:20

... Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation Journal of Translational Medicine 2010 8:137 Submit your next manuscript to BioMed Central and take full advantage of: • ... within samples For example, goat pAb reacted weakly with only one form of gelsolin in the CSF whereas rabbit pAb recognized strongly two forms of gelsolin in that sa...

Ngày tải lên: 18/06/2014, 16:20