determination of cox 2 selectivity in human blood

... Journal 27 3 (20 06) 5333–5346 ª 20 06 The Authors Journal compilation ª 20 06 FEBS Y Ding et al 24 25 26 27 28 29 30 31 32 33 34 35 36 Yoshida T (1995) Heme oxygenase -2 Properties of the heme complex of ... HO -2 contains the cysteine and proline (CP) motifs [23 ], whereas HO-1 lacks a cysteine residue [24 ,25 ] Each CP motif of HO -2 may function as a heme-binding site [26 ], suggesting that HO -2 may sequester ... may in uence the expression of HO-1 The dominant expression of HO -2 protein, in comparison with the low expression of HO-1 protein, in H146 small cell lung cancer cells is of particular interest...

Ngày tải lên: 19/02/2014, 05:20

... effect of cholesterol depletion by MbCD Cell Sample Donor/ epitope Acceptor/ epitope FRET efficiency E (% ± SEM) Kit 225 K6 Kit 225 K6 Kit 225 K6 Kit 225 K6 Kit 225 K6 Kit 225 K6 Kit 225 K6 Kit 225 K6 CD48 CD48 CD25 ... line is a human T cell with a helper/ inducer phenotype and an absolute IL -2 requirement for its growth, while its subclone, Kit 225 IG3, is IL -2 independent [23 ] The IL -2 independent HUT102B2 ... CD25 CD25 CD25 CD25 CD48 CD48 CD25 CD25 HLA-DR HLA-DR CD71 CD71 CD71 CD71 12. 6 2. 3 31 .2 16.3 13.6 14.1 2. 1 1.9 + MbCD + MbCD + MbCD + MbCD ± ± ± ± ± ± ± ± 1.9 1.5 0.9 1.1 2. 2 2. 6 0.8 1.1 In order...

Ngày tải lên: 17/03/2014, 23:20

... 18 19 20 21 22 23 24 25 26 27 28 29 Suh HS, Choi S, Khattar P, Choi N, Lee SC: Histone deacetylase inhibitors suppress the expression of inflammatory and innate immune response genes in human ... mPGES -2 is expressed in activated, but not resting microglia of humans The highest dose of Akt inhibitor X significantly increased PGE2 and PGD2, indicating that a little enhancement in COX- 2 expression ... Findings We show that inhibition of Akt by the Akt inhibitor X enhanced the production of PGE2 and PGD2 without affecting the expression of COX- 2, mPGES-1, mPGES -2 and cytosolic prostaglandin...

Ngày tải lên: 19/06/2014, 22:20

... on structural fixings installed in concrete or masonry used in building and civil engineering 1 .2 Type of test The test consists of applying a shear force to a fixing installed in a solid base ... of fixings The test is intended for expanded and undercut anchors, bonded fixings, cast -in fixings and channel inserts 2. 3 cast -in fixings anchor bolts and sockets cast into the material during ... representative section of the base material in place (see 5 .2) 4.1 Shear testing apparatus, being a loading frame incorporating a means of applying load through a block of steel having a diameter equal...

Ngày tải lên: 08/07/2014, 22:20

... proliferation of SGC7901 cells (Figure 2A) As shown in Figure 2B, down-regulation of COX- 2 might inhibit the malignant growth of SGC7901 cells in vivo Down-regulation of COX- 2 inhibited angiogenesis of ... property of COX- 2 in endothelial cells, the in vitro tube formation of HUVEC was assessed As shown in Figure 4, 5, Figure Down-regulation of COX- 2 suppressed growth of gastric cancer cells in vitro ... differentially expressed in COX- 2downregulating cells, including FGF4, PDGF-BB, PDGFRB, PF4, TGFB2, TGFBR1, VEGF, FLT1, FLK 1, angiopoietin-1, angiopoietin -2, Tie2, IFNA1, PRL, PTN, SCYA2, SPARC, TNFSF15,...

Ngày tải lên: 10/08/2014, 10:21

... staining of Cox- 2 in the gastric carcinoma: the positive expression of COX- 2 protein was stained as yellow or brownish yellow in the cytoplasm of carcinoma cells (LsAB, ×400) The correlation of ... of gastric carcinoma was highlighted by immunostaining against D2-40 (LsAB, 20 0) B Immunohistochemical staining of D2-40 in the intratumoral tissue of gastric carcinoma (LsAB, 20 0) C Immunohistochemical ... staining of D2-40 is similar to that of the COX- 2 staining at a dilution of 1:100 Evaluation of immunohistochemical staining Fifty-six patients with histologically proven gastric adenocarcinoma...

Ngày tải lên: 10/08/2014, 10:21

... potent COX- 2 inhibitor, etodolac, attenuated cough reflex sensitivity to inhaled capsaicin in patients with sinobronchial syndrome This is the first report indicating the modulating role of COX- 2 in ... by COX- 2 inhibition may affect our result as shown in previous study [5] Recently, Kamei and their colleagues [21 ] reported the effect of COX- 2 inhibition in cough reflex sensitivity in guinea ... but its expression is markedly induced in inflammation It can be induced by various stimuli, including inflammatory cytokines, resulting in further production of inflammatory substances such as...

Ngày tải lên: 13/08/2014, 10:20

... Milgrom, E (1999) 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Sequential cleavage and excision of a segment of the thyrotropin receptor ectodomain J Biol Chem 27 4, 101–107 Graves, P., Pritsker, ... periods of times (0–60 min) at 37 °C The concentration of the biotinylated Ig remaining on the cell surface was then quantified by measuring the binding of [ 125 I]Streptavidin In the absence of bTSH, ... domain and devoid of any tag was not functional [27 ], while a mutant containing four amino acids of the ectodomain and including a rhodopsine tag was found constitutively activated [29 ] In agreement...

Ngày tải lên: 21/02/2014, 00:20

... Mandelbaum, A., Etinger, A & Mechoulam, R (19 92) Isolation and structure of a brain con- 20 21 22 23 24 25 26 27 28 29 30 31 32 stituent that bind to the cannabinoid receptor Science 25 8, 1946–1949 ... 20 -fold higher than those of anandamide In fact, after LPS-induced maturation, the amounts of 2- AG were increased 2. 8-fold, as in the mouse macrophage J774 cell line [24 ] and in rat circulating ... domain of the protein of the human and rat CB2 receptor The specificity of the CB1 and CB2 antibodies was described in McIntosh et al [41] and in Shire et al [ 42] , respectively Rabbit anti-human...

Ngày tải lên: 22/02/2014, 07:20

... Journal 27 6 (20 09) 13981417 ê 20 09 The Authors Journal compilation ê 20 09 FEBS S Paravisi et al 42 43 44 45 46 47 48 49 50 utilizing the principle of proteindye binding Anal Biochem 72, 24 825 4 Laemmli ... rate of formation of [32P]ATP increased at increasing concentrations of [32P]PPi (Fig 4) As expected from the observed inhibitory effect of PPi on the tRNA charging reaction, the velocity of [32P]ATP ... for scintillation counting [32P]PPi ATP exchange assay Incorporation of [32P]PPi into ATP was determined in reaction mixtures containing 100 mm Hepes NaOH, pH 7 .2, mm ATP, 16 mm MgCl2, mm l-glutamate,...

Ngày tải lên: 07/03/2014, 03:20

... Hauser, G (1987) Modulation of rat brain cytosolic phosphatidate phosphohydrolase: effect of cationic 27 64 K A Cadwallader et al (Eur J Biochem 27 1) 23 24 25 26 27 28 29 30 31 32 33 34 35 amphiphilic ... inhibit protein kinase C [ 42] It is possible therefore that some of the effect observed with propranolol reflected protein kinase C inhibition Characterization of the PLD isoforms present in human ... the PX domain of p40 (phox) Nat Cell Biol 3, 679–6 82 Karathanassis, D., Stahelin, R.V., Bravo, J., Perisic, O., Pacold, C.M., Cho, W & Williams, R.L (20 02) Binding of the PX domain of p47 (phox)...

Ngày tải lên: 07/03/2014, 15:20

... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 24 8 25 4 26 Wang, W & Malcolm, B.A (1999) Two-stage PCR protocol allowing introduction of ... group of GHMP kinases in which proton transfer does not play a major role in the rate determining step of catalysis That five of the 10 disease-causing mutations resulted in insoluble protein in ... one in which galactose is the first substrate to bind [21 ,22 ] Product inhibition studies were undertaken with recombinant human galactokinase in order to see which mechanistic class it falls into...

Ngày tải lên: 08/03/2014, 02:20

... ample Index complete the book Believing in a Divine Architect of the human form, the author can not refrain from occasionally pointing out His inimitable workmanship, and impressing the lesson of ... jaw, which is hinged [Footnote: A ring of cartilage is inserted in its joints, something after the manner of a washer in machinery This follows the movements of the jaw, and admits of freer motion, ... marrow, rich in fat, and full of blood vessels If we examine a thin slice with the microscope, we shall see black spots with lines running in all directions, and looking very like minute insects These...

Ngày tải lên: 15/03/2014, 13:20

... encodes an active protein kinase and the Cb2 peptide antibody is specific for human Cb2 (A) Human 29 3T cells were transiently transfected using plasmids encoding Ca1 (Ca1) and Cb2 (Cb2) or the cells ... pull-down of larger cell structures, protein clusters or A-kinase A IP: Anti-C 2 Anti-Cmono cAMP P IR IgG - + + + + S P S P S 50 C 2 Cα1 37 B Kinase activity (pmol ATP/mL/min) dH2O cAMP PKI 5 12 22 5 ... anti-Cb2(SNO103) In addition, this revealed that Cb2 was distributed in the cytosol as well as enriched in the centrosome as judged by the anti-c tubulin staining when merged with the anti-Cb2 staining...

Ngày tải lên: 16/03/2014, 18:20

... ª 20 05 FEBS D J Timson 18 19 20 21 22 23 24 25 26 27 28 novel trypanocides Mol Biochem Parasitol 126 , 173– 180 Maitra US & Ankel H (1971) Uridine diphosphate-4keto-glucose, an intermediate in ... utilizing the principle of protein-dye binding Anal Biochem 72, 24 8 25 4 Wang W & Malcolm BA (1999) Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using ... Journal 27 2 (20 05) 6170–6177 ª 20 05 The Authors Journal compilation ª 20 05 FEBS D J Timson Disease-causing mutations in human GALE Fig Crosslinking of wild-type and disease-causing mutants of UDP-galactose...

Ngày tải lên: 23/03/2014, 11:20

... J.RubbRes of Malaysia (20 03) (4): 22 1 -23 0 51 Kaida, K., Determination of Dry Rubber Content of Hevea Latex by Microwave Technique Petanika (19 82) 5 (2) :1 92 52 Chin Hong Cheaw., Plrs" Bull Rubb Res Ins! ... Rubber Research Institute of India, Kottayam (20 00) 14: 24 9- 26 0 14 Southorn, WA and Vip, E., Journal of the Rubber Research Institute of Malay (1968) 20 (4) :20 1 -21 5 15 Low, F.C., Journal of the Rubber ... (1914) 2: 225 -23 0 and 314 - 322 67 Smith, H Fairfield, Specific Gravity of Latex and of Rubber, J Rubb Res of Malaysia, (1940) 21 8 68 Smith, H Fairfield, Journal of Rubber Research Institute of Malaysia...

Ngày tải lên: 23/03/2014, 21:21

... the human homolog of the Drosophila white gene, is a regulator of macrophage cholesterol and phospholipid transport Proc Natl Acad Sci USA 20 00, 97:817- 822 21 22 23 24 25 26 27 28 29 30 31 32 33 ... LXR- 623 Vehicle LXR- 623 (0.1% DMSO) (2uM) (0.1% DMSO) (2uM) 2 Actin 43 kDa 22 0 kDa ABCA1 68 kDa ABCG1 24 hrs 48 hrs Figure treatment of human PBMC ex vivo significantly increases protein levels of ... Activation Reduces Proinflammatory Cytokine Expression in Human CD4-Positive Lymphocytes Arterioscler Thromb Vasc Biol 20 06, 26 :1 022 -1 028 Hindinger C, Hinton DR, Kirwin SJ, Atkinson RD, Burnett ME,...

Ngày tải lên: 18/06/2014, 15:20

... findings in blood that NSC23766 inhibits platelet aggregation due to inhibition of secretion NSC23766 inhibits ADP-induced aggregation of platelets in blood and PRP The extent of inhibition of ... concentrations of NSC23766 would be needed to inhibit Rac1 in platelets in blood considering the possible binding of the drug to plasma proteins and other blood cells and its crossing of the cell ... in collagen- than in TRAP-induced dense granule secretion of platelets in blood These results are in line with a previous study of mice PRP showing the important role of the integrin aIIbb3 in...

Ngày tải lên: 18/06/2014, 16:20

... H23 H26 H27 H29 HCV20 HCV21 HCV51 HCV60 HCV64 HCV66 HCV 72 HCV99 39 54 20 30 49 23 24 29 28 31 25 21 25 46 48 42 42 43 42 29 36 27 32 41 Adult Adult 34 Adult 78 39 28 29 20 25 56 59 57 68 32 55 Adult ... 45 120 2594 45 120 1641 45 120 1714 45 120 4950 45 120 1157 45 120 120 8 45 120 5577 45 120 9889 45 120 3054 13183 127 13 125 77 13410 125 73 13177 13 322 H1 H2 H4 H6 H7 HCV7A HCV7B H8 HCVG8 H9 H14 H17 H18 H20 H23 H26 ... Uru66 Uru 72 Uru99 [EMBL:AM269 927 ] [EMBL:AM269 928 ] [EMBL:AM269 929 ] [EMBL:AM269 926 ] [EMBL:AM269930] [EMBL:AM269931] [EMBL:AM2699 32] [EMBL:AM269936] [EMBL:AM269937] [EMBL:AM269 925 ] [EMBL:AM269933]...

Ngày tải lên: 18/06/2014, 18:20

... 15-desoxy-delta 12, 14-PGJ Ret time (min) Precursor ion Product ion DP FP CE CXP 12. 3 12. 4 12. 7 13.0 13.4 13.9 14.4 14.5 14.7 14.8 15.3 16 .2 353 .2 353 .2 353 .2 351 .2 351 .2 351 .2 351 .2 438 .2 333 .2 333 .2 335 .2 ... 309 .2 309 .2 309 .2 315 .2 315 .2 333.3 333.3 333 .2 315.1 315.1 195 .2 271.1 -66 -66 -66 -36 -36 -26 -26 -14 -36 -36 -31 -45 -310 -310 -310 -170 -170 -150 -150 -70 -20 0 -20 0 -150 -21 0 -26 -26 -26 -16 ... concerning pulmonary diseases for different disciplines in medicine Chemicals Prostaglandin D2 (PGD2), 13,14-dihydro-15-keto-PGD2, 11β-PGF2α, PGJ2, ∆ 12- PGJ2, PGF2α, 13,14-dihydro-15keto-PGF2α, PGE2,...

Ngày tải lên: 20/06/2014, 00:20