diversity of natural anti α galactosyl antibodies in human serum

... CLEANING PROCEDURES sensors 3-aminopropyl-triethoxy-silance (APTES) Experimetal immobilizations of anti JEV serum APTES – Antibody - Immobilization with mg/ml anti- JEV antibodies contained human serum ... with anti- JEV antibodies contained serum (1mg/ml in PBS pH 7.0) (C) - The sensor coated with APTES, added 10% glutaraldehyde (G.A) and then immobilized with anti- JEV antibodies contained serum ... signal of the sensor using APTES – 10% glutaraldehyde for immobilization of anti- JEV antibodies contained human serum is 2- to 3- fold higher than other methods The response time of JEV antigen...

Ngày tải lên: 15/04/2015, 09:20

... previously incubated with 10 µg of recombinant human nucleophosmin Anti- NPM antibodies are present in patients with SLE and are associated with aCL antibodies The demonstration that anti- NPM antibodies ... no reactivity against NPM was observed Similarly, offspring of NZB and NZW mice also did not develop anti- NPM antibodies In contrast, anti- NPM antibodies were present in 60% of 20 sera from MRLlpr/lpr ... produced in male WB mice prompted us to search for antiNPM antibodies in the sera of 82 patients with SLE Indeed, 23 (28%) of these sera were positive for anti- NPM antibody (Fig 4) and the antibodies...

Ngày tải lên: 09/08/2014, 07:20

... evaluated in clinical trials (at a dosage of 52.5 mg) It was subsequently withdrawn from clinical testing as a result of increased risk of sexually transmitted infection due to disruptions in the vaginal ... cervical-vaginal tissues before being considered as a candidate for evaluation in clinical trials It must not cause inflammation or sloughing of the vaginal epithelium, and even minor, subclinical ... vitro algorithm In each assay where natural lemon or lime juice was used, the cytotoxicity observed dominated the activity profiles of the juices, providing minimal separation of antiviral efficacy...

Ngày tải lên: 10/08/2014, 05:21

... serum anti- α- actinin antibodies in lupus patients was associated with a 2.5-fold increase in the prevalence of LN [14] Finally, levels of anti- α- actinin antibodies correlated with those of anti- DNA ... an increased binding to α- actinin as compared with patients with no nephritis [12] and that pathogenic human anti- dsDNA antibodies bound strongly to α- actinin [12,13] Moreover, an increase in serum ... of αactinin antibodies in this cohort also has to be considered since in previous studies about 20% of SLE patients had Page of (page number not for citation purposes) increased anti- α- actinin...

Ngày tải lên: 12/08/2014, 11:22

... pathways involved in viral expression include protein kinase C, calmodulin and intracellular calcium mobilisation More physiological stimuli of viral expression include cross-linking of membrane ... J, Buehring GC: In vivo protein binding and functional analysis of cis-acting elements in the U3 region of the bovine leukemia virus long terminal repeat J Virol 1998, 72:5994-6003 Dekoninck A, ... became a standing dogma There are however a series of caveats in this model Indeed, the maintenance of a vigorous anti- viral immune response in infected animals indicates that some degree of virus...

Ngày tải lên: 13/08/2014, 09:20

... analysis using anti- caspase-8, anti- Bid, anti- Bax, anti- HSP60, antiphospho-Bcl-2, anti- Bcl-2 and anti- b-actin serum HSP60 and b-actin were used as a mitochondrial marker protein and a loading control, ... using anti- cytochrome c and anti- a-tubulin serum The total cell extract was subjected to western blot analysis using anti- phospho-JNK, anti- JNK, anti- caspase-3 and anti- b-actin serum a-tubulin ... analysis using anti- caspase-8, anti- Bid and anti- b-actin serum b-actin was used as a loading control (D) Effect of inhibition of JNK on Bax and Bak activation HeLa cells were treated with 10 Gy of c-radiation...

Ngày tải lên: 07/03/2014, 05:20

... N-terminal processing of CGA in rat heart extracts Our data reveal the presence of several vasostatin I-containing and vasostatin II-containing peptides in the CGA(1–199) domain, strongly supporting ... Processing of proenkephalin-A in bovine chromaffin cells Identification of natural derived fragments by Vasostatin-containing peptides in rat heart 57 58 59 60 61 62 63 64 65 66 67 N-terminal sequencing ... of chromofungin, the antifungal chromogranin A (47–66)-derived peptide Ann N Y Acad Sci 971, 359– 361 Aardal S, Galindo E, Aunis D & Helle KB (1993) Human chromostatin inhibits endothelin-1-induced...

Ngày tải lên: 07/03/2014, 12:20

... presence of a tyrosine in strand C of CCP4 in place of an asparagine in C1r CCP2 (N379), and based on the results of the present perturbation study, we suggest that the inter-CCP flexibility in C1r ... that occur in diverse proteins Thus, constructs containing domains and their combinations that were different from those of the modular C1r protein were expected to be easily obtainable in folded ... the proteases Intermodular and intramodular flexibilities are thought to be essential in these movements Expression of recombinant intact C1r [4] and its domain combinations in insect cells and...

Ngày tải lên: 15/03/2014, 23:20

... price increase in labour or material inputs Not taking account of loss of life Not taking account of indirect effects Not taking account of increases in exposure This led to the following effects ... Appraisal of Increase in banana Expected return negative strengthening of roots of banana trees yields in years with as expected yields against windstorms windstorms decreased, but increase in stability ... function of hazard intensity The degree of direct and indirect damage is increasing with increasing flood depth Often, the lack of availability of such fragility is a major constraint in risk and damage...

Ngày tải lên: 16/03/2014, 02:20

... matching peptides using the same database and search engine Mass determination of proteins was done with the same instrument operated in linear mode and externally calibrated with a mixture of ... Additional binding sites were blocked by incubation of the nitrocellulose blot in 2% skim milk in 20 mm Tris ⁄ HCl, pH 7.4 for h The blot was incubated overnight with chicken antibodies against the ... shown in Fig 6, no band was detected in the postnuclear supernatant of eosinophils, but a band at a molecular mass of 42 kDa was detected in the postnuclear supernatant of neutrophils, indicating...

Ngày tải lên: 16/03/2014, 04:20

... Modulation of HSA ligand binding by anti- HIV drugs Table Values of intermolecular energies (kJÆmol)1) obtained from the docking simulation of the three anti- HIV drugs in the heme binding cavity ... proximity of FA7) [51] Indeed, nevirapine binds to its primary binding site at Kd $ · 10)6 m (Supplementary material) By looking at Fe(III)heme binding Modulation of HSA ligand binding by anti- HIV ... electron–nucleus interactions, including anisotropies of the g tensor, the zero-field splitting tensor, and the hyperfine coupling tensor [62–64] Interestingly, binding of nevirapine to HSA determines a...

Ngày tải lên: 23/03/2014, 07:20

... amounts of protein A 100-fold increase in mRNA level does not increase protein in an equivalent proportion because of the limited capacity of the synthesis machinery, especially for membrane proteins ... terminator region of RNA polymerase II Close inspection revealed that the mRNA of OCT1h was predominantly terminated upstream of the EBNA-1 binding site region With pEBTetD, the copy number of ... for pEBTetD cell pools in continuous culture up to 10 weeks indicate useful factors of inducibility on the level of protein function Clearly, in many situations, e.g in RNA interference experiments,...

Ngày tải lên: 23/03/2014, 09:21

... characterize serum TNX we generated antibodies against nonoverlapping FNIII repeats starting at the N-terminus of the C-terminal 100-kDa portion of TNX, as shown in Fig 1A Using our new set of antisera, ... and TNX proteins were diluted in T-NaCl ⁄ Pi containing 0.1% BSA Multiple species of tenascin-X in human serum Binding of TE to serum TNX was performed as described above Briefly, serum TNX was ... FEBS 1287 Multiple species of tenascin-X in human serum D F Egging et al secondary antibodies (conjugated to biotin) against rabbit (Brunschwig Chemie) and antibiotin antibodies (conjugated to...

Ngày tải lên: 23/03/2014, 09:21

... N-terminus of Ets-1 and the C-terminus of Elk-1 were involved in the formation of the heterodimer Preincubation of nuclear proteins with antibodies against Ets-1 or Elk-1 prevented the formation of ... its C-terminal domain is involved in DNA binding [38] By contrast, the DNA-binding domain of Elk-1 is present at its N- terminal end and C-termini allows it to form homo- or heterodimers In our ... used an antibody against Ets-1 raised against its N-terminal region and an antibody against Elk-1 raised against phosphorylated Ser383 present at the C-terminus of this protein Preincubation of nuclear...

Ngày tải lên: 29/03/2014, 09:20

... regulator of the PAR-1 gene and a competitor of AP-2 for binding to the regulatory region of the PAR-1 gene [19,29] Interestingly, in the human alveolar epithelial A549 cell line, Sp1 inhibition, as in ... downregulation of PAR-1 gene expression However, ongoing protein synthesis is required for maintaining the level of PAR-1 on the cell surface Counts Fig Involvement of PKA and Epac in PGE2-induced downregulation ... factors in PAR-1 expression and the possible in uence of PGE2 on their activity For the analysis of Sp1 involvement, we used its specific inhibitor mithramycin A This drug interferes with Sp1 binding...

Ngày tải lên: 30/03/2014, 04:20

... Antonio Negri have called for in our own century During this time of intimidating potential, the architects of decolonization carried out the exhilarating work of imagining independent states Du Bois ... mentioned in Looking Backward, publishing One of “Berrian’s” Novels in 1890 The vast majority of these books are long since out of print, with only five titles still commonly read The remainder of this ... those utopias still set in far-off “undiscovered” lands As proof of the dominance of developmentalist thinking during this period, we will find it within utopian novels often held to be utterly...

Ngày tải lên: 10/06/2014, 21:24

... first report of these findings in HPIV1 The distribution of amino acids affects the structure of the HN coding region in HPIV1, and previous reports show that substitutions of amino acids in HN glycoprotein ... hemagglutinin-neuraminidase (HN) glycoprotein and fusion protein (F protein) [1] HN glycoprotein shows multiple biological functions that include hemagglutinin and enzymatic activities as neuraminidase ... distance of each of the minor clusters in the trees The tree was constructed by neighbor-joining analysis with labeling of the branches showing at least 70% bootstrap support The representation of...

Ngày tải lên: 18/06/2014, 18:20

... condition in the gas injection well The above equations are in oil field units in which d is expressed in inches, L in feet,  in centipoises, T in degrees rankine and pressure in pounds per square inches ... Day Tubing internal diameter (d) = 1.9956 in Gas gravity (G g) = 0.6 Depth = 5790ft (bottom of casing) Temperature at foot of tubing (T w f) = 16 0oF Surface temperature (T s f) = 8 3oF Tubing head ... (2009) In the original problem the pipe was horizontal In the modification used in this work, the pipe www.intechopen.com Static behaviour of natural gas and its low in pipes 457 was made to incline...

Ngày tải lên: 27/07/2014, 23:44

... perpetuation of joint inflammation Potential direct anti- inflammatory effects of A77 1726 on joint cells are thus of interest because of their relevance to the effectiveness of leflunomide in treating ... novo synthesis of pyrimidines [7,8] The inhibition of DHODH activity by A77 1726 might explain part of its mechanism of action in suppressing inflammation Indeed, many effects of A77 1726 can ... inhibition of COX-2 activity, then the effect of indomethacin on IL-1Ra production should be comparable to that of A77 1726 Indeed, indomethacin increased IL-1β-induced IL-1Ra secretion in human synovial...

Ngày tải lên: 09/08/2014, 01:23