facilitation of protein three-dimensional structure determination using enhanced peptide amide deuterium exchange mass spectrometry (dxms)

... compilation ê 2009 FEBS 1401 Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution Stefano Paravisi 1 , ... minor, implying rotations of domains of just a few degrees (e.g. 7° interdomain rotations upon tRNA binding to the Tt-GluRS and local limited rear...

Ngày tải lên: 07/03/2014, 03:20

... Calcium and peptide binding to folded and unfolded conformations of cardiac troponin C. Electro- spray ionization and Fourier transform ion cyclotron resonance mass spectrometry. Eur J Mass Spectrom ... thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass...

Ngày tải lên: 07/03/2014, 17:20

... C-terminal site of the dimerization and docking domain. This could allow the interdomain/cAMP binding region to pivot about the stable AKAP-bound dimerization and docking domain, and to weakly associate with ... subcellular compartments by A -Kinase Anchoring Proteins (AKAPs). The site of interaction with AKAPs is the N-terminal docking and dimerization...

Ngày tải lên: 08/03/2014, 22:20

... 2006 The Authors Journal compilation ê 2006 FEBS 4927 Antioxidant Dps protein from the thermophilic cyanobacterium Thermosynechococcus elongatus An intrinsically stable cage-like structure endowed ... exploited during the purification of Dps proteins. To investigate Dps ther- mostability, a protein from the thermophilic cyano- bacterium Thermosyn...

Ngày tải lên: 16/03/2014, 12:20

... Malaysia and the south of Thailand. Parkia platycephala is an important forage tree growing in parts of north-eastern Brazil. The seed lectin from Parkia platycephala is a 47.9-kDa single-chain ... Parkia speciosa [14], Parkia javanica [15], Parkia discolor [16] and the glucose ⁄ mannose-specific lectin from Parkia platycephala seeds [17–21]. Parkia (Legum...

Ngày tải lên: 16/03/2014, 13:20

... of the combined amide I and II bands, with separate analyses of each band afterwards; and separate normalization of the amide I and II bands. All these normalizations were tested with and without ... reflect the natural abundances found in the PDB. The spectra of the 50 proteins are available on request from the authors. Protein secondary structure tab...

Ngày tải lên: 16/03/2014, 18:20

... mixtures of XAIP and GH11 xylanase and XAIP and GH13 a- amylase were analysed. The prominent peaks corresponding to complexes of XAIP with GH11 xylanase and GH13 a- amylase were observed in each case. ... that XAIP inhibits the activity of a- amylase in a 1 : 1.2 molar ratio. The inhibition of a- amylase by XAIP was also observed in the presence of GH11 xylanase...

Ngày tải lên: 29/03/2014, 09:20

... CGTTCTTAGAAGCGGTGCATTTTCC PAF K3 5A opafK35Ase GTTTGATAACAAGGCTTGCACCAAGG pPic9Kmpaf opafK35Arev CCTTGGTGCAAGCCTTGTTATCAAAC PAF K3 8A opafK38Ase GAAGTGCACCGCTGATAATAACAAATG pPic9Kmpaf opafK38Arev CATTTGTTATTATCAGCGGTGCACTTC PAF K35,3 8A opafK35,38Ase ... CATTTGTTATTATCAGCGGTGCACTTC PAF K35,3 8A opafK35,38Ase GTTTGATAACAAGGCTTGCACCGCTG pPic9KpafK3 8A opafK35,38Arev CAGCGGTGCAAGCCT...

Ngày tải lên: 29/03/2014, 23:20

... Research Open Access Research article Study of the collagen structure in the superficial zone and physiological state of articular cartilage using a 3D confocal imaging technique Jian P Wu* 1 , ThomasBKirk 1 ... physiological status of the cartilage in the scoring system of International Cartilage Repair Society. Results: Normal arti...

Ngày tải lên: 20/06/2014, 01:20

... purposes) Respiratory Research Open Access Research Psoriasin, one of several new proteins identified in nasal lavage fluid from allergic and non -allergic individuals using 2-dimensional gel electrophoresis and ... 5.3 Proteins in bold are newly identified in NLF. Table 1: Identified proteins in nasal lavage fluid from allergic...

Ngày tải lên: 12/08/2014, 18:22

... al.: Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis. Clinical Proteomics 2011 8:1. Submit your next manuscript ... extraction, in -gel proteolytic diges- tion and identification of proteins via tandem mass spectrometry analysis. Here, we measured the proteomic profile...

Ngày tải lên: 13/08/2014, 13:20

... UNIVERSITY OF CALIFORNIA, SAN DIEGO FACILITATION OF PROTEIN 3-D STRUCTURE DETERMINATION USING ENHANCED PEPTIDE AMIDE DEUTERIUM EXCHANGE MASS SPECTROMETRY (DXMS) A dissertation ... by Enhanced Amide Hydrogen -Deuterium Exchange Mass Spectrometry (DXMS). Blood 100:259a. xviii xix ABSTRACT OF THE DISSERTATION FA...

Ngày tải lên: 13/11/2014, 16:12