chemoproteomic characterization of protein kinase inhibitors using immobilized atp

... mitochondria of Su9-DHFR, in which the presequence of ATPase subunit of Neurospora crassa was fused to a passenger protein, DHFR As seen in Fig 5A, the level of mitochondrial targeting of Su9-DHFR, ... Role of PKA in the mitochondrial targeting of CYP2E1 The role of PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches The first approach involved measuring the level of ... Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 Naresh B...

Ngày tải lên: 18/02/2014, 16:20

... FEBS analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40]. COFRADIC analysis of protein processing ) protease degradomics Protein processing introduces novel protein fragments that ... features of ATP-binding proteins. As expected, the majority of FSBA-labeled Jurkat proteins were known binders of small nucleotides, cofactors, or DNA and RNA molecules. However, several proteins ... FSBA-targeted or FSBG-targeted proteins. Overlaying an image of the immunoblot with the 2D pattern of silver-stained proteins pointed to candidate ATP-binding or GTP- binding proteins that were selected...

Ngày tải lên: 18/02/2014, 16:20

... isomerase activities of cPDI measured using conotoxins as substrates. Oxidase a,b Moles of reduced tx3a per mole of oxidase per min (· 10 )2 ) Isomerase b,c Moles of nTx3.1 per mole of isomerase per ... dithiols of reduced polypeptide. We ana- lyzed the oxidase activity of cPDI using reduced tx3a (20 lm) as substrate. As shown in Fig. 3, the conotoxin Z Q. Wang et al. Characterization of PDI from ... expression and characterization of protein disulfide isomerase from Conus marmoreus Zhi-Qiang Wang 1 , Yu-Hong Han 1,2 , Xiao-Xia Shao 1 , Cheng-Wu Chi 1,2 and Zhan-Yun Guo 1 1 Institute of Protein Research,...

Ngày tải lên: 07/03/2014, 05:20

... atoms: Ct of the C-terminus, Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt of the N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending ... phospholipids, or cAMP In turn, protein kinases or protein phosphatases alter the phosphorylation state of specific subcellular targets [1] Because many protein kinases are of broad specificity, a key ... dephosphorylation of protein targets in the cell are mediated by specific protein–protein interactions [2,3] The cAMP-dependent protein kinase (PKA) is tethered to specific subcellular compartments by A-Kinase...

Ngày tải lên: 08/03/2014, 22:20

... sequence of plant calcium-dependent protein kinases resembles that of animal protein kinases C, in this study, we used the Xenopus laevis oocyte protein kinase C to identify the target sites of calcium-dependent ... Modification of T306 abolished KAT1 activity in oocyte recordings, whereas modifica- tion of T308 did not cause a loss of function [21]. In animal cells, one type of Ser ⁄ Thr protein kinase, protein kinase ... AAPK ⁄ ABR kinase has been shown to phos- phorylate the C-terminal region of KAT1 [16]. One of the 10 members of the SNF1-related protein kinase 2 in A. thaliana, SnRK2.6, is an ortholog of AAPK...

Ngày tải lên: 22/03/2014, 21:21

... Autophosphorylation of the catalytic subunit of cAMP-dependent protein kinase J Biol Chem 267, 25174–25180 Yonemoto W, McGlone ML & Taylor SS (1993) N-myristylation of the catalytic subunit of cAMPdependent protein ... the catalytic subunit of the cAMP-dependent protein kinase J Biol Chem 258, 3693–3701 30 Zhang W, Morris GZ & Beebe SJ (2004) Characterization of the cAMP-dependent protein kinase catalytic subunit ... cAMP-dependent protein kinase Eur J Biochem 268, 5066–5073 Kvissel AK, Orstavik S, Oistad P, Rootwelt T, Jahnsen T & Skalhegg BS (2004) Induction of Cbeta splice variants and formation of novel forms of protein...

Ngày tải lên: 30/03/2014, 04:20

... function of CK2 in these cells but could reflect inhibitor-induced uncoupling of DNA damage response decay from break ligation. Introduction Protein kinase CK2 is a ubiquitous and highly conserved protein ... Page 10 of 13 [...]... article as: Zwicker et al.: A specific inhibitor of protein kinase CK2 delays gamma-H2Ax foci removal and reduces clonogenic survival of irradiated ... One-thousand -and- one substrates of protein kinase CK2? FASEB J 2003, 17:349-368 4 Sarno S, Ghisellini P, Pinna LA: Unique activation mechanism of protein kinase CK2 The N-terminal segment...

Ngày tải lên: 09/08/2014, 09:20

... measurements of the Mb system.The concentration of Mb was 1.5 × 10 -5 mol/L. (A) ACF of Mb vs. the concentration of DMSO; (A’) Size distribution histogram of Mb vs. the concentration of DMSO; (B) ACF of ... concentration of Q. (A’) Zeta potential of Lys vs. the concentration of DMSO; (B’) Zeta potential of Lys vs. the concentration of Q. (A’’) Zeta potential of Mb vs. the concentration of DMSO; (B’’) ... ACF of Lys vs. the concentration of Q; (B’) Size distribution histogram of Lys vs. the concentration of Q. The concentration of DMSO was maintained at 10% in B and B’. Fang et al. Journal of Nanobiotechnology...

Ngày tải lên: 11/08/2014, 00:23

... Journal of Inflammation 2010, 7:16 http://www.journal-inflammation.com/content/7/1/16 Open Access RESEARCH Characterization of protein tyrosine phosphatase H1 knockout mice in animal models of local ... duration of the immune response is under stringent regulation Tyrosine phosphorylation is a central mechanism in the control of key signaling proteins involved in innate immunity The role of protein ... detected the withdrawal of the paw Latency of each paw withdrawal was recorded and mean values of left and right paws were used as reaction index for the individual animal A cut-off of 25 seconds was...

Ngày tải lên: 11/08/2014, 03:20

... STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMORESISTANCE Bibek Parajuli Submitted to the faculty of ... 59 SAR by CB29 class of compounds 59 SAR by CB7 class of compounds 63 F Crystal structures of inhibitors with ALDH3A1 66 Crystal structure of ALDH3A1 with CB29 ... .113 B Selectivity of CB29 for ALDH3A1 versus ALDH1A1 and ALDH2 117 C Characterization of CB7 binding 123 D Probing CB7 binding of ALDH3A1 site using Q122A and Q122W mutants...

Ngày tải lên: 24/08/2014, 09:51

... CHARACTERIZATION OF INTERFACIAL MECHANICAL PROPERTIES USING WEDGE INDENTATION METHOD YEAP KONG BOON NATIONAL UNIVERSITY OF SINGAPORE 2010 CHARACTERIZATION OF INTERFACIAL MECHANICAL ... Table of Contents Preface i Acknowledgements iii Table of Contents iv Summary viii List of Tables x List of Figures xii List of Symbols xix Chapter 1: Introduction 1.1 Overview of ... supervision of Associate Professor, Dr. Zeng Kaiyang. Part of the research works have been conducted at Institute of Materials Research and Engineering, Singapore (IMRE). To the best knowledge of the...

Ngày tải lên: 11/09/2015, 09:56

... Ms. Zhu Jing, PhD student of Associate Prof. Dr. Zeng Kaiyang. 160 Chapter Section 7.2 presents the calculations of the interfacial toughness of the RuO2/Si system, using the wedge indentation ... student* using DC reactive... fracture processes * Courtesy of Ms Zhu Jing, PhD student of Associate Prof Dr Zeng Kaiyang 160 Chapter 7 Section 7.2 presents the calculations of the interfacial ... Calculations of the interfacial toughness of the RuO2/Si system (120° wedge indentation) Notes: (a) The average value of. .. ε = 0 .8 The errors are standard deviations of the data...

Ngày tải lên: 11/09/2015, 09:56

... metalloprotease AGC family protein kinase A, protein kinase G and protein kinase C AKAP A-kinase anchoring proteins aPKC atypical protein kinase C AR androgen receptor ATP adenosine 5’-triphosphate ... humans, a total of 518 protein kinases have been identified including 478 human eukaryotic protein kinases and 40 atypical protein kinases. The protein kinases constitute about 1.7 % of all human ... the type of amino acid phosphorylated, the protein kinases can be divided into two subgroups: protein serine/threonine kinases and protein tyrosine kinases. Serine/threonine protein kinases were...

Ngày tải lên: 12/09/2015, 21:10

... Assessment of Protein Interaction Data using Graph Topology Approaches Jin Chen B.C.Sc. (Hons) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY SCHOOL OF COMPUTING NATIONAL UNIVERSITY OF SINGAPORE ... Assessment of Protein Interaction Data using Graph Topology Approaches by Jin Chen, B.Eng. Dissertation Presented to the Faculty of the School of Computing of the National University of Singapore ... Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY National University of Singapore October 2006 Systematic Assessment of Protein Interaction Data using Graph Topology...

Ngày tải lên: 14/09/2015, 18:02

... in 25ml of milli-Q water and the pH of the whole medium was adjusted to pH 8.0). 225 µl of NaDC tris buffer, 2.5µg of protein and different concentrations of inhibitors such as THL 44 (tetrahydrolipstatin) ... atoms were restrained. A production run of 10 nano second was used to check for the stability of protein models which is a plot of root mean square deviation of the backbone structure versus time. All ... 2.5µg/µl of protein concentration and 1mM of pnP-C2/C4/C16 substrate incubated for 30mins at 37 ºC. The final volume of each well was kept at 250 µl and the absorption was measured at 400nm using...

Ngày tải lên: 02/10/2015, 17:15

... grains, the five groups of PDI family proteins remained in the aleurone cells and the protein matrix of the starchy endosperm Conclusions: High expression of PDI family proteins during grain filling ... cloning of cDNAs encoding bread wheat PDI family proteins of groups I-V (TaPDIL1A, TaPDIL1B, TaPDIL2, TaPDIL3A, TaPDIL4D, and TaPDIL5A) and characterization of the enzymatic properties of their ... Figure S7) Expression and characterization of recombinant wheat PDI family proteins To investigate the enzymatic properties of wheat PDI family proteins, recombinant proteins without the putative...

Ngày tải lên: 26/05/2020, 23:59

... or the panel of high expression of these proteins was an independent prognostic factor for tumor recurrence The combination of high expression of these proteins with a high level of CA19–9 could ... analyzed using the combination of high expression of these proteins with primary tumor (T) or lymph node metastasis (N) status Conclusion: EGFR, HER4, EphA3 or the panel of high expression of these proteins ... Padthaisong et al BMC Cancer (2020) 20:154 Page of 16 Table The correlation of 12 protein kinases and post-operative recurrence of CCA patients Protein kinases Early recurrence Late recurrence No...

Ngày tải lên: 17/06/2020, 03:22

... Open Access Protein kinase C inhibitor Gö6976 but not Gö6983 induces the reversion of E- to Ncadherin switch and metastatic phenotype in melanoma: identification of the role of protein kinase D1 ... allowed the identification of a novel serine/threonine kinase, i.e protein kinase Cμ, also known as protein kinase D1 (PKD1), whose specific inhibition allows the reversion of the metastatic phenotype ... atypical member of the protein kinase C family J Biol Chem 1994; 269(8):6140–8 24 Valverde AM, Sinnett-Smith J, Van Lint J, Rozengurt E Molecular cloning and characterization of protein kinase D: a...

Ngày tải lên: 20/09/2020, 18:59

... Deininger M, et al The price of drugs for chronic myeloid leukemia (CML) is a reflection of the unsustainable prices of cancer drugs: from the perspective of a large group of CML experts Blood 2013;121(22):4439–42 ... Vilnius, Lithuania Clinics of Oncology and Hematology, Hospital of Lithuanian University of Health Sciences Kauno Klinikos, Eivenių g 2, 50009 Kaunas, Lithuania Department of Oncology Haematology, ... countries Herein we report the entire country data on incidence and survival of CML, as well as penetrance of tyrosine kinase inhibitors (TKIs) in Lithuania Methods: We analyzed all patients (N = 601)...

Ngày tải lên: 21/09/2020, 09:23

... Growth of the pancreatic cancer cell line PANC-1 is inhibited by protein phosphatase 2A inhibitors through overactivation of the c-Jun N-terminal kinase pathway Eur J Cancer 2011, Epub ahead of print ... inhibition of pancreatic cancer cells through G2/M cell-cycle arrest and apoptosis Cancer Sci 2010, 101(5):1226–1233 Millward TA, Zolnierowicz S, Hemmings BA: Regulation of protein kinase cascades by protein ... RESEARCH ARTICLE Open Access Development of a gene therapy strategy to target hepatocellular carcinoma based inhibition of protein phosphatase 2A using the α-fetoprotein promoter enhancer and pgk promoter:...

Ngày tải lên: 05/11/2020, 07:50