levels of protein structure

... of the studies of APC levels in human sepsis are not all that obvious and may even seem contradictory In the study of de Kleijn and colleagues in children with meningococcal sepsis, the APC levels ... value of protein C concentrations in neutropenic patients at high risk of severe septic complications Crit Care Med 2000, 28:22092216 Gruber A, Griffin JH: Direct detection of activated protein ... the APC and PC levels and ratios On the other hand, we need these kinds of studies to determine the exact role of APC and PC in severe sepsis and the possible therapeutic role of PC and APC supplementation...

Ngày tải lên: 12/08/2014, 23:21

... the protein Structural properties of modules Modularity of protein function We carried out the modular decomposition of protein structures of a non-redundant dataset of 100 multi-domain proteins ... 40 50 60 70 Protein number 80 90 100 110 Figure conformers Modular3flexibility for each protein of the dataset of proteins with Modular flexibility for each protein of the dataset of proteins with ... 50 60 Protein number 70 80 90 100 Figure of long-range interactions for each protein of the multi-domain protein dataset Percentage Percentage of long-range interactions for each protein of the...

Ngày tải lên: 14/08/2014, 07:21

... adaptability of 3DBLAST for the initial scan of large protein structure databases; conversely, detailed structure alignment tools often align two structures slowly but accurately Because of basic ... SCOP-108 the distributions search searchlisting 976 proteins23 of X-ray structures on proteins forof the representative 3D structure codes 38 proteins structures overview representative segments structedkind ... the statistical significance (E value) of an alignment, indicating the reliability of a hit protein structure For the purposes of scanning a large protein structure database, 3D-BLAST is fast and...

Ngày tải lên: 14/08/2014, 18:20

... structured forms of proteins and are often employed in studies aimed at clarifying aspects of the folding process of proteins [34], including cytochrome c [18] Concerning this last protein, fast ... the protein in the presence of FEBS Journal 273 (2006) 5347–5357 ª 2006 The Authors Journal compilation ª 2006 FEBS F Sinibaldi et al Anion-modulated structure of cyt c A-state A B Fig Effect of ... (B) CD spectra of acid-denatured cytochrome c in the presence of 0.02 M sulfate (—) and 0.02 M selenate (— ÆÆ— ÆÆ) The spectra of the native (-Æ-Æ-) and of the denatured (ÆÆÆÆ) protein are shown...

Ngày tải lên: 19/02/2014, 05:20

... atoms: Ct of the C-terminus, Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt of the N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending ... 24 NMR structures of our final ensemble of dimer structures of RIIa D/D Dielectric smoothing at the protein solvent interface [20,21] was used, with an ion exclusion layer around the ˚ protein ... ensemble of 24 structures, the lowest energy and the best (smaller rmsd from the mean) structure of RIIa D/D Each monomer of RIIa D/D consists of a disordered N-terminal segment Fig Superposition of...

Ngày tải lên: 08/03/2014, 22:20

... High levels of disorder in scaffold proteins Fig Schematic representation of the domain structure of selected scaffold proteins The scheme shows the domain architecture of 20 selected scaffold proteins ... definition of these proteins In this article, we focus on three classes of complex-forming proteins of related function, also including anchor and docking proteins The prototype for anchor proteins ... disordered ⁄ unstructured proteins (IDPs ⁄ IUPs), the concept of protein disorder has gained general recognition recently [3–6] Physical evidence exists for the disorder of about 500 proteins [7],...

Ngày tải lên: 16/03/2014, 02:20

... natural deconvolution of circular dichroism curves of proteins Protein Eng 4, 669–679 ´ Dousseau, F & Pezolet, M (1990) Determination of the secondary structure content of proteins in aqueous solutions ... Determination of protein secondary structure using factor analysis of infrared spectra Biochemistry 29, 9185–9193 21 Byler, D.M & Susi, H (1986) Examination of the secondary structure of proteins ... the PDB The spectra of the 50 proteins are available on request from the authors Protein secondary structure tabulation from DSSP output The secondary structures of the RaSP proteins were determined...

Ngày tải lên: 16/03/2014, 18:20

... Although atomic resolution structures of the molecular packing of peptides in amyloid-like structures are beginning to emerge [19], much of our understanding of the structure of amyloid-like fibrils ... that mishandling of glucagon solutions of > gÆL)1 leads to the formation of toxic aggregates [78] Moreover, a recent report comparing the toxicity of amyloid structures of several protein hormones ... prevalence of different glucagon fibril structures is the result of a multitude of aggregation pathways, where even small shifts in environmental conditions can impact the outcome of the struggle...

Ngày tải lên: 23/03/2014, 03:20

... oligodendrocytes express extremely low levels of mRNA for C1-inh and significantly lower levels of mRNA for MCP compared with astrocytes and microglia The expression level of mRNAs for CD59 and DAF showed ... thiocyanate-phenol-chloroform method Two µg of the RNA was then used to prepare cDNA RNA was treated with 10 U of DNase I (Gibco BRL, Life Technologies) for 60 at 37°C in 25 µl of × reverse transcriptase ... were of the predicted size (see Table 1) Statistical analysis The data are presented as means ± s.e.m The significance of difference between values was estimated by means of one-way analysis of...

Ngày tải lên: 19/06/2014, 22:20

... serum levels of anti-ribosomal P protein antibodies were found in 44.3% (31/70) of autistic patients Patients with elevated serum neurokinin A levels had significantly higher serum levels of anti-ribosomal ... increased serum levels of anti-ribosomal P protein antibodies were found in 44.3% of autistic patients This study was the first to investigate serum levels of anti-ribosomal P protein antibodies ... serum levels of neurokinin A and CARS in autistic patients, P < 0.001 This may indicate that the extent of the elevation of serum neurokinin A was closely linked to the degree of the severity of...

Ngày tải lên: 19/06/2014, 22:20

... histogram of aspect ratio (1 μm × μm) (b) of the as-grown sample Dashed lines in (a) present the Gaussian fitting of PL peak Inset of (a) gives sketch of sample structure and inset of (b) gives ... (°C) Figure Annealing temperature dependence of integrated intensity of QDs and InGaAs QW (a), peak energy of GS and ES1 of QDs (b), FWHM of GS and ES1 of QDs (c) firstly and then quenches when ... accompanying with series of oscillations caused by built-in electric field The two weak peaks at the low energy regions can be attributed to the energy levels of the complex structure of In0.15Ga0.85As...

Ngày tải lên: 21/06/2014, 04:20

... patients in medication, CRP levels or antibody profiles (not shown) In two of the anti-CRP-positive sera, signs of weak nonspecific IgG binding to the plastic surface of the microtitre plates were ... However, the very low CRP levels seen in SLE patients with glomerulonephritis, often contrasting with levels of other acutephase reactants, might in fact be due to the consumption of CRP by ICs [35] ... binding Available online http://arthritis-research.com/content/6/2/R87 The levels of anti-CRP did not correlate with the levels of anti-nucleosome antibodies (r = 0.0136), indicating that anti-CRP...

Ngày tải lên: 09/08/2014, 01:23

... CSF levels of TGF-β, another amyloidogenic protein with anti-inflammatory properties In the CNS lupus group, intrathecal levels of TGF-β were significantly increased compared with those of SLE ... β-amyloid protein (Aβ42) have not been assessed at all in this condition Protein tau is a microtubuli-associated protein that promotes assembly and stability of microtubuli [20] Elevated CSF-tau levels ... protein (APP) [22], a large transmembrane protein Numerous studies have found increased CSF-tau levels, decreased CSF-Aβ42 levels but variable levels of CSF-APP in AD [22–25] Transforming growth...

Ngày tải lên: 09/08/2014, 01:23

... thylakoid structure was typiof cal of sunny habitats, since the ratio of the length of appressed to non-appressed thylakoid membranes remained below 1.4 (Fig B) The leaves examined from levels and ... thylakoid structure typical of shade plants (Lichtenthaler et aL 1981Part of this reorganization in thylakoid membranes might also be due to ageing, since the area of plastoglobuli of chloroplast ... chloroplast ultrastructure, and leaf characteristics of high- and low-light plants and of sun and shade leaves Photosynth Res 2, 115-141 light E.M (1986) Correlation of activity and amount of ribulose...

Ngày tải lên: 09/08/2014, 03:24

... size of the protein bands of interest The same amount of protein was loaded onto each lane Figure Content of osteogenic protein protein in synovial fluid samples The samples osteogenic protein ... protein (OP-1) and hyaluronan (HA) contents (r2 = 0.1345, P = 0.0654) Interestingly, SF levels of OP-1 tended to correlate positively with levels of HA, but correlate negatively with levels of ... and quantified by the Quantity One Software program (BioRad) Measurement of OP-1 by ELISA For the quantitative assessment of OP-1 protein levels, aliquots of SF samples were first diluted 1:100...

Ngày tải lên: 09/08/2014, 08:22

... electrophoresis using polyclonal rabbit anti-C1q (DAKO, Glostrup, Denmark) Levels of C1q were expressed as the percentage of the levels of healthy blood donors (normal range, 76 to 136%) C3 (normal range, ... membranous lupus nephritis Anti-C-reactive protein antibody levels in patients with proliferative versus membranous lupus nephritis The levels of anti-C-reactive protein (anti-CRP) antibodies in the ... lesions in glomerulonephritis [3] The circulating levels of anti-dsDNA often correlate with disease activity, and these autoantibodies are presumed to be of pathogenetic importance in lupus nephritis...

Ngày tải lên: 12/08/2014, 11:22

... Recently, another study in very early RA patients reported an increase of ACPA levels of 10% and a decrease of IgM-RF levels of 14% [30] The marked decrease found in the present study is probably ... no data are available on changes in levels of ACPA or IgM-RF as a predictor of disease outcome Changes in autoantibody levels could possibly serve as markers of response to therapy and thus be ... http://arthritis-research.com/content/12/1/R8 Page of Absolute change of IgM-RF and ACPA compared with the absolute change of DAS28, HAQ and SHS Figure Absolute change of IgM-RF and ACPA compared with the absolute change of DAS28, HAQ...

Ngày tải lên: 12/08/2014, 11:22

... significant) Median levels are shown by the line Discussion In the present study, we found elevated levels of both IL-17A protein and mRNA levels and also of IL-23 mRNA in sputum of clinically stable ... p

Ngày tải lên: 12/08/2014, 13:22

... high levels of SP-D also produce high levels of the anti-inflammatory cytokine IL-10 and high levels of IL-6 In contrast, Balb/c mice release significantly more KC and IL-12p70 Elevated Page of ... numbers of lymphocytes, eosinophils and macrophages [4,6] Altered levels of surfactant protein D have been reported in association with a number of different pathological conditions of the lung ... elevation of SP-D protein levels in the airways, with a peak 48 hours after allergen challenge [15] In accordance to that, SP-D protein levels were still increased at 48 and 72 hrs when IL-6 levels...

Ngày tải lên: 12/08/2014, 16:20

... The levels of the hRasGRP4 transcripts were higher than the normal levels in 41% of our RA patients (p < 0.0001) (Figure 2) The levels of the hRasGRP4 transcript also were higher in the PBMCs of ... nonfunctional protein in terms of P44/42 mitogen-activated protein (MAP) kinase activation Conclusions: The identification of defective isoforms of hRasGRP4 transcripts in the PBMCs of RA patients raises ... isoforms of hRasGRP4 caused by alternative splicing of its precursor transcript (Figure 3) Four previously identified isoforms of hRasGRP4 have been designated as splice variants to [1] Two of...

Ngày tải lên: 12/08/2014, 18:20