... - 0,5 M Tris-Cl pH 6,8 - 0,4% SDS - Tris base 15,14 g - H2O 200 ml - - 10 % SDS 10 ml - 250 ml ... - m 607 acid amin. - - 66463Da (= 66,5 kDa) - oC: 4,7 - 824 M -1 cm -1 279 nm - ... - Acrylamide 58, 42 g - Bis-acrylamide 1,6 g - H2O dn t t n 200 ml 6. - Coomassie R250 1 g - Glacial acetic acid 100 ml (10%) - Methanol 400 ml (40%) - H2O...