Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells docx
Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells docx
... neuronal cells. J Biotechnol 2006, 122:393-411. doi: 10.1186/1423-0127-17-44 Cite this article as: Lin et al., Sonoporation-mediated gene transfer into adult rat dorsal root ganglion cells Journal ... conditions for gene transfer by sonoporation require further investigation. Conclusions This study demonstrates that sonoporation enables deliv- ery of plasmids into...
Ngày tải lên: 10/08/2014, 05:21
Altered neuronatin expression in the rat dorsal root ganglion after sciatic nerve transection ppsx
... date, no study has examined the altered expression of Nnat in dorsal root ganglion (DRG) after axotomy of sciatic nerve in adult rats. This research study was there- fore conducted using Western ... increased in total protein extracts. Unmyelinated C-fiber and thinly myelinated A-δ fiber in adult dorsal root ganglions (DRGs) were the principal sub- population of primary afferen...
Ngày tải lên: 10/08/2014, 05:21
Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... the rat dorsal root ganglion- derived cell line F-11, a hybridoma of mouse neuroblastoma (N18TG2) and rat dorsal root ganglion cells. We found by RT-PCR that F-11 cells express not only the rat ... analysis. Bender et al. [9] isolated a rat VRL-1 mRNA from F-11 cells identical to that observed in dorsal root ganglion extracts and suggested that the F-11 cells m...
Ngày tải lên: 31/03/2014, 07:20
Báo cáo khoa hoc:" Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems" pptx
... Transfection of non-dividing cells may be obtained by activating nuclear uptake by inserting nuclear localiza- tion signals into the transgene [13,14]. Adenoviral gene transfer into osteoclasts has been ... suggest- ing that efficient gene transfer is as a sum of many positively affecting parameters. Inside cells, DNA needs to escape from the endosomes before their maturation...
Ngày tải lên: 11/08/2014, 08:20
báo cáo hóa học:" SERCA2a gene transfer improves electrocardiographic performance in aged mdx mice" docx
... Zsebo K, Jeong D, Park WJ, Kawase Y, Hajjar RJ: Restoration of mechanical and energetic function in failing aortic-banded rat hearts by gene transfer of calcium cycling proteins. J Mol Cell Cardiol ... muscular dystrophy. Hum Gene Ther 2009, 20:1319-1328. 12. Bostick B, Yue Y, Lai Y, Long C, Li D, Duan D: Adeno-associated virus serotype-9 microdystrophin gene therapy ameliorates ele...
Ngày tải lên: 20/06/2014, 04:20
Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
... Sakashita, M. & Tanaka, S. (1994) Lysophosphatidic acids induce pro- liferation of cultured vascular smooth muscle cells from rat aorta. Am. J. Physiol. 267, C204–C210. 27. Green, L.C., Wagner, D.A., ... Yamada, N. (2000) Effect of hypoxia on nitric oxide production and its synthase gene expression in rat smooth muscle cells. Biochem. Biophys. Res. Commun. 268, 329–332. 29. Saura,...
Ngày tải lên: 22/02/2014, 04:20
báo cáo khoa học: "Antiproliferative factor decreases Akt phosphorylation and alters gene expression via CKAP4 in T24 bladder carcinoma cells" docx
... incorporation by as- APF (APF) in cells electroporated with no siRNA, CKAP4 siRNA, or non-target siRNA. Results are shown as percent inhibition of 3 H- thymidine incorporation compared to control cells ... [uM] % Inhibition of 3 H-Thymidine Incorporation Figure 1 CKAP4 knockdown in T24 cells. A, Western blot analysis of CKAP4 protein expression in cells electroporated in the presence o...
Ngày tải lên: 10/08/2014, 10:20
Báo cáo sinh học: " Genetic incorporation of the protein transduction domain of Tat into Ad5 fiber enhances gene transfer efficacy" docx
... purposes) Ad5.PTD tat exhibited enhanced gene transfer efficacy in a variety of tumor cellsFigure 4 Ad5.PTD tat exhibited enhanced gene transfer efficacy in a variety of tumor cells. Gene transfer efficacy was ... expressing U118MG cells [43,44]. Enhanced gene transfer efficacy of Ad5.PTD tat We further investigated the gene transfer efficacy of Ad5.PTD tat in a vari...
Ngày tải lên: 18/06/2014, 18:20
Báo cáo hóa học: " Genetic incorporation of the protein transduction domain of Tat into Ad5 fiber enhances gene transfer efficacy" ppt
... purposes) Ad5.PTD tat exhibited enhanced gene transfer efficacy in a variety of tumor cellsFigure 4 Ad5.PTD tat exhibited enhanced gene transfer efficacy in a variety of tumor cells. Gene transfer efficacy was ... for in vivo gene delivery into tumors, which is essential for can- cer gene therapy. Discussion In this study, we sought to improve the gene transfer effi-...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo y học: "Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees" potx
... was generated by Cre- Lox-driven recombination in Cre 8 cells [22]. Briefly, a confluent 10 cm 2 dish of Cre 8 cells (1.6 × 10 7 ) was split into five 6 cm 2 dishes. Transfection of these cells ... JE, Evans CH, Robbins PD: Direct retrovirus-mediated gene transfer to the synovium of the rabbit knee: implications for arthritis gene therapy. Gene Ther 1997, 4:977-982. 17. Mi Z,...
Ngày tải lên: 09/08/2014, 01:21