gene transfer to plants

Construction of nerve growth factor loop 4 containing polypeptides for facilitated gene transfer to neurons

... vectors As there is no panacea, there is no versatile vector for gene therapy to all kind of diseases Gene delivery vector has to be customized to fit the requirement of various diseases To target ... ligands into the vectors to direct therapeutic gene to new cell surface receptors Depending on the targeted receptors, various ligands have been used to conjugate nonviral vectors Targeted gene delivery ... The genes transferred include suicide genes and genes encoding cytokines, antigens, tumor suppressors, markers and receptors etc For updated information on gene therapy trials worldwide, refer to...

Ngày tải lên: 16/09/2015, 17:13

163 177 0

Báo cáo y học: "Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline"

... virus (AAV) vectors have been used to gene target somatic cells at high frequencies Hirata et al used AAV vectors to introduce transgenes into the hypoxanthine phosphoribosyl transferase (HPRT) ... into hidden factors has been the notion of the existence of ‘inhibitory’ factors (IFs) associated with sperm cells These IFs are envisaged to prevent exogenous DNA uptake so as to protect the genetic ... attributed to the fortuitous removal or inhibition of IF(s) [43] Seminal fluid reportedly contains an inhibitory factor (IF-1) that appears to actively block the binding of exogenous DNA to sperm and to...

Ngày tải lên: 03/11/2012, 10:01

16 507 1

Báo cáo y học: "Local IL-13 gene transfer prior to immune-complex arthritis inhibits chondrocyte death and matrix-metalloproteinase-mediated cartilage matrix degradation despite enhanced joint inflammation" pps

... IL-4 is regarded as a potent anti-inflammatory cytokine by direct inhibition of proinflammatory cytokines such as IFNγ, IL-1, and tumor necrosis factor α [12] However, IL-4 protein and mRNA are ... intravenously into mice ICA was then passively induced by injecting µg lysozyme coupled to polyL-lysine in µl pyrogen-free saline into the knee joints Histology of arthritic knee joints Total knee ... anti-inflammatory cytokine, which in general reduces IL-1β production [14,27,41] However, the effect of IL-13 on IL-1 production by IC-stimulated macrophages has not been described to date In addition to...

Ngày tải lên: 09/08/2014, 06:22

10 389 0

Báo cáo y học: "Stable gene transfer of CCR5 and CXCR4 siRNAs by sleeping beauty transposon system to confer HIV-1 resistance" doc

... conventional retroviral vectors or lentiviral vectors were used for gene transfer Although highly efficient for stable gene transfer, use of retroviral derived vectors poses a degree of risk ... of developing a non-viral gene delivery of anti-HIV genes for gene therapy, here we evaluated the utility of SB transposon system to deliver siRNA genes for stable gene transfer Two previously ... both CCR5 and CXCR4 coreceptors were used for SB mediated siRNA gene transfer in these proofs of concept studies Since the general gene transfer efficiency is low relative to that typically obtained...

Ngày tải lên: 10/08/2014, 05:21

9 355 0

báo cáo khoa học: " Meiosis-specific gene discovery in plants: RNA-Seq applied to isolated Arabidopsis male meiocytes" docx

... performed to reduce the total number of significant changes This reduced the total number of significantly expressed genes to 12,484 (Figure 4; S vs A = 8,850, S vs M = 9,723, A vs M = 5,216) To find ... investigate the plant meiotic proteome Cytogenet Genome Res 2005, 109(1-3):181-189 Mercier R, Grelon M: Meiosis in plants: ten years of gene discovery Cytogenetic and genome research 2008, 120(3-4):281-290 ... understanding of meiotic genes, gene expression and regulation, especially the transcript profiles of MGI genes and TE genes, and provide a framework for functional analysis of genes in meiosis Background...

Ngày tải lên: 11/08/2014, 11:21

14 192 0

Chemical drug assisted gene transfer a sensible approach to improve transgene expression in the central nervous system

... processes(Factor, 2001) The original goal of gene therapy was to correct a genetic disorder by inserting a functional gene into an organism to replace an inherited defective one However, recently gene ... elements and therapeutic genes The vectors refer to the carriers of transgene into target cells, and they have been commonly divided into viral and non-viral vectors Regulatory elements are DNA ... to focus on the gene delivery vectors for gene therapy in CNS Although an ideal gene delivery vector requires high efficiency with no side effects such as cytotoxicity and immunogenecity, the current...

Ngày tải lên: 12/09/2015, 10:15

161 418 0

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Thu thập nguồn gene và tổ chức dữ liệu gene 1

... THO PhD TRI BUI MINH PhD VU NGUYEN CONG The purpose of this research is to collect, orgarnize gene data to surve research in genetic modified plant The research was carried out at the Chemical and ... GenInfo Indentifier Gln Glutamine GM plant Genetic modified plant GMO Genetic modified organism HTML hypertext markup language HTTP hypertext transfer protocol ID identify J2EE Java Enterprise Edition ... đƣợc chép DNA (gene) 12 Hình 1.11: Cắt DNA Plasmid sử dụng enzyme cắt giới hạn 13 Hình 1.12: Gắn gene chuyển vào vector (Plasmid) 13 Hình 1.13: Plasmid dùng chuyển gene đậu nành...