agrobacterium tumefaciens a natural gene transfer system

... client-server architecture, for use with a general-purpose natural language understanding system The conversion of resources such as Comlex and WordNet into a format usable by our system was straightforward, ... information about the semantic structure of concepts associated with English words, particularly verbs For example, the verb abridge has an associated case frame consisting of an agent doing the abridging ... such as theme and goal We developed a semi-automatic tool which examined every verb which had rules in the semantics server, and based on the lexical entry for that verb, added additional semantic...

Ngày tải lên: 20/02/2014, 18:20

... inhibition) AMP–AMP phosphotransferase reaction in human colorectal mucosa from cancer patients Table shows the activities of AdK, MK, ADA and AMP–AMP phosphotransferase in normal and cancerous human ... purchased from ABNOVA Corporation (Taipei, Taiwan) All other chemicals and HPLC solvents were of analytical grade and were acquired from Merck KGaA (Darmstadt, Germany) and J T Baker Italia (Milan, ... activity was restored every time we combined the two separate protein preparations ADA was also isolated When ADA was added to the assay mixture, AMP– AMP phosphotransferase activity was greatly...

Ngày tải lên: 23/03/2014, 06:20

... vivo XII ABBREVIATION AAV Adeno-associated virus ABC ATP-binding cassette AcMNPV Baculovirus Autographa californica multiple nucleopolyhedrovirus Ad Adenovirus BBB Blood-brain-barrier BV Baculovirus ... sclerosis, as well as brain tumors such as neuroblastoma and Glioblastoma multiforme (GBM) (Hsich et al., 2002) This is largely undesirable because these diseases are potentially amenable to gene therapy ... electroporation, and particle bombardment, which are generally used for naked DNA delivery Chemical methods use a large range of chemical agents such as lipids and polymers as carrier molecules that...

Ngày tải lên: 12/09/2015, 10:15

... husband, David, is a bass player Back in the mid-’80s, he was playing a benefit to save Broadway theaters, which were then being demolished at an alarming rate As he was waiting to go on and a number ... manager insisted on it As a supervisor, Ryan now had to give in-person reports to top management on a regular basis Every time his manager asked for an advance look at Ryan’s presentation, Ryan ... people has taught me never to assume that simply acquiring or refining a particular skill will mean that all is immediately and always well Using what you’ve learned about yourself so far as a guide...

Ngày tải lên: 27/01/2014, 08:20

... Systems, ed P Jacobs, Lawrence Erlbaum Associates, Hillsdale, NJ, pp 13-33 Kameyama, M (1992) "The Syntax and Semantics of the Japanese Language Engine", forthcoming In Mazuka, R., and N Nagai, ... the fact that every lexical item has a semantics associated with it Table contains average edge counts and parse timing statistics for the 5875-utterance training set Many systems (Carbonell and ... overhead associated with adding types to the grammar The major grammatical category plays a special role in the typing scheme of Gemini For each category, Gemini makes a set of declarations stipulating...

Ngày tải lên: 20/02/2014, 21:20

... vocabulary serving as a base for applying semantic criteria, becat, se the overall core vocabulary was envisaged to have a size of approx 1500 words Inspection shows that many intuitively basic ... best cmc can do" What one can hope to do, is to define For a natural language system which is supposed to analyze and generate texts, to engage in dialogues with users, and which is to acquire ... that both statistical mclhods and semanlic criteria can, and I think, have to contribute to the establishment of a core vocabulary The work on fimnulating taxonomic relati(,ns, semantic constraints...

Ngày tải lên: 22/02/2014, 10:20

... in Natural Language Generation: Friend or Foe? Invited talk at the European Workshop for Natural Language Generation, Toulouse, France Reiter, Ehud and Robert Dale 2000 Building Natural Language ... to be more portable across subject domains and languages, as long as those resources are available for the targeted domains and languages FrameNet in particular allows us to generate multiple possibilities ... Press, Cambridge, MA Shemtov, Hadar 1997 Ambiguity Management in Natural Language Generation, PhD Thesis, Stanford Kaplan, R M and J Wedekind 2000 LFG generation produces context-free languages...

Ngày tải lên: 17/03/2014, 06:20

... logical order and shows on demand the details of each type of data • Enable access to a variety of database query capabilities ETE stores all types of intermediate data as strings in the database ... application Matrix Comparison Analysis of NLU Systems A typical NLU system takes an input of certain form and produces a final as well as a set of intermediate analyses, for instance, parse treesl ... Provide graphical and contextual information for annotation Annotation is a problem because it still takes a human ETE offers flexible and easy access to the intermediate data within and across batch...

Ngày tải lên: 17/03/2014, 07:20

... nominal "laboratory animal", and animals can have a disease, and thus transmit it, a. s seen in the phrase "animals implicated in hepatitis" Similarly, the only relevant fact about "water" is that ... all of these are aspects of a disease "laboratory animals", "laboratory spores", "laboratory contamination", "laboratory nwthods" "a study by a research laboratory", "laboratory testing", "laboratory ... laboratories The second rests on the fact that information is acquired there • "infectious disease": A disease can be transmitted A classical issue in lexical semantics that arises at this stage...

Ngày tải lên: 17/03/2014, 19:21

... achieve a state (STATEX) In assumption 1, (STATEX) is in fact specialized for a consultation system as a real world state (instead of a mental state which is the general assumption in any dialog system) ... the proposal is (implicitly) anticipated, and that modifications of a want or of a proposal are not manageable In a more elaborated version, the goal accomplishment has to be marked as provisory ... drawn from a user want referring to a state, missing his/her acquaintance with the associated causal relation (pursuing a global goal) - 32 - (WANT SYSTEM (KNOW SYSTEM X FUTURE)NOW) A (BELIEVESYSTEM...

Ngày tải lên: 24/03/2014, 05:21

... relations of a sentence But what about the logical relations? Recall that each clause has a unique head end that the functional features of each phrase are identified with those of its head For ... property that the functional features of each phrase are identified with those of its head The head category of a phrase is characterized by d~e assignment of the trivial ft~%ctional-equation and by ... predicates which are goals ca~ give infon~tion to their subgoals In short, once a phrase structure grammr has been translated into a PROIOG pragraune every node is potentially able to grasp information...

Ngày tải lên: 24/03/2014, 05:21

... ´ C J Almeciga-Dıaz et al Although bone marrow transplantation improves many aspects of the somatic manifestations, it has a limited impact on cardiac, eye and skeletal abnormalities, in addition ... Mizukami H, Kume A, Muramatsu S, Takeuchi K, Matsushita T, Okada T, Kobayashi E, Hoshika A & Ozawa K (2004) Adeno-associated virus (AAV) vector-mediated liver- and muscle-directed transgene expression ... by intracerebral AAV-mediated delivery of sulfamidase and SUMF1 genes Hum Mol Genet 16, 2693–2702 Kurai T, Hisayasu S, Kitagawa R, Migita M, Suzuki H, Hirai Y & Shimada T (2007) AAV1 mediated co-expression...

Ngày tải lên: 29/03/2014, 21:20

... Adams WT ( 1981 ) Inheritance of isozyme variants in seed tissues of balsam fir (Abies balsamea) Can J Bot 59, 1285-1291 Neale DB, Adams WT (1985) Allozyme and matingsystem variation in balsam ... other Abies: A alba, = 0.89 (Schroeder, 1989); A lasiocarpa, m t m t 0.89 (Shea, 1987); A balsamea, t m 0.89 (Neale and Adams, 1985) This is in good concordance with phenological and biological ... fixed in the maternal parent and nearly fixed in the pollen pool In addition, a preliminary analysis indicated that five loci were poorly behaved and were omitted for the mating system analysis With...

Ngày tải lên: 08/08/2014, 18:21

... displays annual growth rings (Lowe, 1968; Paliwal and Prasad, 1970; Lawton and Lawton, 1971; Rao, 1972; Amobi, 1973; Paliwal et al, 1976; Ghouse and Shamima Hashmi, 1979; Zamski, 1979; Rao and Dave, ... wood formation, especially the lack of rainfall, as in the wood of Carya glabra where the spacing of tangential parenchyma bands is related to the rate of rainfall (Hill, 1982) At any rate, the ... l’Arbre Nat Monspeliensia, 303-337 Dave YS, Rao KS (1982) Seasonal activity of the vascular cambium in Gmelina arborea Roxb IAWA Bull 3, 59-65 Deshpande BP, Rajendrababu T (1985) Seasonal change...

Ngày tải lên: 08/08/2014, 23:22

... mean diameter of axes was measured with a calliper rule: main stems at cm above and below each tier, and different branches at the base a tape A complete set of data was collected for each of ... similar to that in some tropical and temper- ate woody species, such as Persea americana, Pinus taeda, Populus deltoides (Bor- Tabernaemontana crassa (Prévost, 1972) and to the radial growth rate ... branches for metabolites apply only to T superba plants in the early stage and in a favourable natural environment was were The differential radial growth of the "destem also indicated that capitated"...

Ngày tải lên: 08/08/2014, 23:22

... cellular level, the advantage of gene transfer as a means of drug delivery arises from the sustained availability of IL-1Ra that this method permits Materials and method Materials Ham’s F12 medium, ... gene transfer in the treatment of chronic articular disease Indeed, IL-1Ra gene therapy has demonstrated impressive efficacy in animal models of RA and OA, and a phase I human trial has recently ... (Carlsbad, Ca, USA) Recombinant human IL-1β and IL-1Ra were purchased from R&D Systems (Minneapolis, MN, USA) ELISA kits for PGE2 and IL-1Ra were purchased from Dynatech (Ann Arbor, MI, USA) and...

Ngày tải lên: 09/08/2014, 01:23

... cytotoxicity assays and manuscript preparation EHG carried out statistical analysis and manuscript preparation TBG carried out patient referral, clinical data analysis and manuscript preparation MHP carried ... patient referral, data analysis and manuscript preparation All authors read and approved the final manuscript Acknowledgements This work was supported, in part, by NIH grant PO1 AR048929 (to AAG), ... 35:1-14 29 Yabuhara A, Yang FC, Nakazawa T, Iwasaki Y, Mori T, Koike K, Kawa H, Komiyama A: A killing defect of natural killer cells as an underlying immunologic abnormality in childhood systemic lupus...

Ngày tải lên: 09/08/2014, 06:22

... from media was chromatographically separated using PD-10 columns (Phar- macia, Piscataway, NJ, USA), and radiolabeled GAGs released into the culture media or recovered from the alkaline treatment ... rabbit knee (b) Synovium from an AIA rabbit knee that received Ad.eGFP as an inflamed control (c) Synovium from an AIA rabbit knee that was treated with Ad.hIL-10 (d) Synovium from an AIA rabbit ... The animals were acclimatized for three days before experimentation and were fed chow ad libitum and water All animal experiments were conducted in accordance with NIH standards of animal care and...

Ngày tải lên: 09/08/2014, 08:22

... Splink BglII (5'-GATCCATGGTTGTTAGGACCTGGAGGGGAAATCAATCCCCT-3', 5'-phosphate) was used for left IR/DR and splink SphI (5'-GTTGTTAGGACTGCTTGGAGGGGAAAATCAATCAATCCCCT-3', 5'-phosphate) was used for right ... generated by heating equimolar amounts of long primerette (5'-CCTCCACTACGACTCACTGAAGGGCAAGCAGTCCTAACAACCATG-3') with the respective splink to 80°C and cooling it to room temperature Page of (page number ... system plasmid [29] The CCR5 siRNA target sequence is 5'-GUGUCAAGUCCAAUCUAUG-3' whereas the CXCR4 siRNA target sequence is 5'-GAGUCUGAGUCUUCAAGUU-3' The CXCR4 or CCR5 shRNA DNA cassette was generated...

Ngày tải lên: 10/08/2014, 05:21

... enhance cationic liposome-mediated gene therapy J Drug Target 1998, 5:163-169 Tanaka S, Takahashi T, Takayanagi H, Miyazaki T, Oda H, Nakamura K, Hirai H, Kurokawa T: Modulation of osteclast ... that a total loss of osteoclasts occurred already after a h treatment with transfection reagents Adenoviral gene delivery also resulted in osteoclast death and decreased viability, but the majority ... that allows transgene packaging, protection and sufficient bioavailability Conclusion Although many cell lines and some primary cells are easy to transfect using calcium phosphate, DEAE-dextran,...

Ngày tải lên: 11/08/2014, 08:20