... histopatho-logical and in vivo procedures. The question of whether introduction of the bisecting GlcNAc changes the ligand properties (binding affinities) of biantennary N-glycans forbranch-end-specific lectins ... of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo Sabine Andre´1, Carlo Unverzagt2,*, Shuji Kojima3, Martin Frank4, ... affect binding properties. Compared with thesolid-phase assays, the studies of cell binding allowed determination of the ligand properties of biantennary N-glycans [37] with either bisecting GlcNAc...