antitumor effect in vitro and in vivo

... were obtained by venipuncture and separated in aliquots One of them was collected in tubes containing EDTA and assigned to determine haematimetric indexes, plasmatic protein concentration and rheological ... In vitro experiments - Erythrocyte incubation in hyaluronic acid solutions and RI determination Blood samples were obtained from healthy adults by venipuncture and collected in tubes containing ... Consequently, its increase may be explained by the rise in fibrinogen and/ or globulin concentration and/ or to the modification of the erythrocyte surface properties In our in vitro experiments,...

Ngày tải lên: 10/08/2014, 05:21

... characterized by insulin resistance and insulin secretion deficiency At present, there is no a single medication which treats type diabetes by improving both insulin resistance and insulin secretion ... fusion protein exhibited significant glucose-lowering effect in diabetic mice and may be a promising agent that can treat type daibetes by improving both insulin resistance and insulin secretion ... enterokinase cleavage; Lane 2: before enterokinase cleavage Glucose-lowering effect of gAd-GLP-1-A fusion protein We investigated the glucose-lowering effect of gAd-GLP-1-A fusion protein in diabetic...

Ngày tải lên: 25/10/2012, 11:10

... leukemia cells) were obtained from KCLB (Korean Cell Line Bank, Seoul, Korea) and maintained in RPMI medium containing 10% (v/v) fetal bovine serum (FBS, GibcoBRL, Grand Island, NY) in a humidified atmosphere ... L casei and B longum showed high activity on all tumor cells, and led us in vivo antitumor study with these strains Our data showing different antiproliferative activity in these strains consist ... suggesting that both L casei and B longum in intestinal microflora can activate immune system to prevent diseases including tumors References Fig Survival rate of F9-bearing mice after LABs administration...

Ngày tải lên: 07/08/2014, 17:22

... nitrates and nitrites TJ assisted during in vivo and in vitro experiments and did the graphical presentation DS performed statistical analysis and assisted during in vivo experiments DP and RK ... [43] and acid soluble nucleotides were determined spectrophotometrically at 260 nm The defined units for purified DNase I and DNase II (increase in absorbance of 0.001/min in a sample containing ... as U/g protein, for tissue and cell samples The defined units for purified DNase I and DNase II (increase in absorbance of 0.001 / in a sample containing 0.132 mg DNA, pH 7.4 or pH and ml of reaction...

Ngày tải lên: 13/08/2014, 13:20

... shikonin derivative, showed antitumor effects both in vivo and in vitro Xie et al [6] showed that some shikonin derivatives were more powerful than natural shikonin in terms of antitumor effects ... acetylshikonin (I) and shikonin (II) Chemical structures of acetylshikonin (I) and shikonin (II) cells Natural shikonin-like compounds also have significant in vivo antitumor effects In a study ... http://www.cmjournal.org/content/4/1/14 Inhibition rate % Chinese Medicine 2009, 4:14 acetylshikonin μg/ml Figure cells (n = effects of acetylshikonin on the growth of tumor Inhibitory 3) Inhibitory effects of acetylshikonin on...

Ngày tải lên: 13/08/2014, 15:21

... chondrosarcoma cells in vitro by measuring the effect of steroids and clinical drugs inhibiting estrogen-signaling We evaluated responsiveness of central chondrosarcoma cell lines and primary cultures ... chondrosarcoma, but no beneficial effect of inhibiting estrogen signaling both in vitro and in vivo Clinical Sarcoma Research 2011 1:5 Submit your next manuscript to BioMed Central and take full advantage ... addition, in spite of positive immunohistochemical staining for ESR1 protein in all in vitro cell cultures, addition of 17b-estradiol, 4-androstene-3,17-dione or drugs targeting the estrogen-signaling...

Ngày tải lên: 13/08/2014, 15:21

... ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and 1% nonessential amino acids (Gibco Invitrogen) Murine hepatoma-1 ... all-trans-retinoic acid, the findings clearly demonstrate that S-4o9cDH-RA has the capacity to bind and activate nuclear retinoid receptors and regulate gene transcription both in vitro and in vivo Retinoids ... grown in low-glucose DMEM (Gibco Invitrogen) supplemented with 10% (v ⁄ v) fetal bovine serum (Gibco Invitrogen), 1% (v ⁄ v) PEST (Gibco Invitrogen), 1% (v ⁄ v) l-glutamine (Gibco Invitrogen) and...

Ngày tải lên: 18/02/2014, 08:20

... USA) and transformed into E coli for protein induction Extracts prepared from cells induced for h were shown to contain a six-histidine C-terminal-tagged OsRPA14 fusion protein The OsRPA14 protein ... tag were obtained by in vitro translation in wheatgerm extract (Invitrotec) according to the manufacturer’s procedure OsRPA70a (or OsRPA70b) and OsRPA32, or OsRPA32 and OsRPA14 were incubated with ... soluble in the presence of m urea Soluble fractions were loaded on to columns containing 10 mL His-Bind resin (Novagen) The columns were washed with 50 mL binding buffer containing m urea and then...

Ngày tải lên: 07/03/2014, 21:20

... shown) In conclusion, we have shown using both in vitro and in vivo methods, that PSI-G adopts a topology in the thylakoid membrane with the loop facing the stroma and its N- and C-termini facing ... promoter and terminator, and into vectors for in vitro transcription and translation An Arabidopsis line (psag-1.4 [5], termed DG in this report), lacking PSI-G due to a transposon footprint in exon ... after 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D...

Ngày tải lên: 07/03/2014, 21:20

... CPS and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... Interresidue NOEs were observed between H-1a and H-3c and between H-1b and H-3a, suggesting a linear structure a-c-b (Fig 2E,F) In addition, interresidue NOEs between H-1c and H-4b (Fig 2G) indicated ... and O-chain polysaccharide of A salmonicida strain 80204-1 the previously identified O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and...

Ngày tải lên: 23/03/2014, 13:20

... the protein The self-cleavage of LexA protein is important in the SOS response in bacteria [21–23] In vitro self-cleavage was also observed in all investigated bacterial SPase I including enzymes ... results intrigued us to investigate the cleavage of S pneumoniae SPase I in vivo As shown in Fig 6C, Western blot analysis demostrated that the self-cleavage of SPase I is indeed occuring in vivo in ... the interaction between the enzyme and the cytoplasmic membrane In this study, we were interested in investigating the effect of phospholipid on the self-cleavage of S pneumoniae SPase I In the...

Ngày tải lên: 23/03/2014, 21:21

... nonspecific binding by protein–protein interactions, cells were incubated with 100 lg ligand-free carrier protein (BSA) per mL for 30 at °C before incubation with the biotinylated neoglycoprotein in Dulbecco’s ... assay, saturable and carbohydrate-dependent binding was measured, and the resulting Scatchard plots gave straight lines indicating the presence of a single class of binding sites in each case (not ... neoglycoproteins, we determined their binding to cells and to tissue sections as well as their biodistribution in mice after injection of radioiodinated material Binding to cells and organs In these...

Ngày tải lên: 30/03/2014, 13:20

... evaluated in cell culture and in intranasally infected mice The deletion of F2L had a minimal impact on viral replication in vitro and did not appear to significantly reduce the virulence in vivo ... repeatable increase in the efficacy of N-methanocarbathymidine (N-MCT) (Table 2) This compound is a carbocyclic thymidine analog that inhibits the replication of VV both in vitro and in vivo [12,13], and ... replication in vivo In HSV, deletion of the dUTPase homolog did not effect the replication of the virus in vitro, however its virulence was reduced by 1000-fold in mice following footpad inoculation and...

Ngày tải lên: 20/06/2014, 01:20

... against JEV using mouse model After ascertaining the in vivo non-toxic concentrations in preliminary experiments, the therapeutic potential of SCH 16 was evaluated in mice using intracerebral and ... reports describing the in vitro inhibitory effect of compounds such as ribavirin, mycophenolic acid, imino sugars, inhibitors of serine protease, RNA interference and non-steroidal anti-inflammatory ... depicting the in vivo effect of SCH 16 against a lethal JEV challenge Survival graphs depicting the in vivo effect of SCH 16 against a lethal JEV challenge SCH 16 was administered to swiss albino...

Ngày tải lên: 20/06/2014, 01:20

... J, Ince N, Ouyang N, Min J, Chen J, Shankar P, Lieberman J: RNA interference targeting Fas protects mice from fulminant hepatitis Nat Med 2003, 9:347-351 Gitlin L, Karelsky S, Andino R: Short interfering ... growing in a monolayer, and having a well-defined tendency for parallel orientation Viral infection causes a marked CPE resulting in total cellular detachment, rounding, and destruction, which ... double-stranded RNA (dsRNA) is a very powerful tool for the inhibition of virus replication and has a high therapeutic potential In our case, the inhibition effect is not so well-defined as in the...

Ngày tải lên: 20/06/2014, 01:20

... in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol and ... Akt and Erk signalling pathways in mediating the effects of the gonadotropins and IGF on follicle cell function and on follicular development The stimulation of inhibin-A, activin-A, follistatin, ... previously in the bovine model Increases in Akt and Erk signalling proteins in response to FSH and IGF stimulation suggest a role for Akt and Erk signal transduction pathways in FSH and IGF mediated...

Ngày tải lên: 20/06/2014, 07:20

... distribution in these last two studies was similar to what was obtained with the i.v injected 11 1In- labeled panitumumab F(ab’)2 already discussed Discussion The in vivo and in vitro properties of the intact ... radiolabelings TN performed PET imaging and analysis MWB synthesized the chelate and participated in the design of the studies DEM conceived of and coordinated the studies and assisted with the in vivo ... 48-96 h post-injection [13] The objective of this study was to evaluate the in vivo and in vitro properties of panitumumab F(ab’)2 fragment and to assess its utility as a targeting agent in radioimmunodiagnostic...

Ngày tải lên: 21/06/2014, 02:20

... cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing 10% FCS, 100 U/ml of penicillin, and 100 μg/ml of streptomycin ... and fixed in 100% methanol for 10 Cell monolayers were blocked in 5% BSA in PBS for 45 and incubated for h at room temperature with P-gp antibodies (Invitrogen, Beijing, China), followed by incubation ... were suspended in 100 μL of culture medium and subcutaneously inoculated into the right flank of mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study...

Ngày tải lên: 21/06/2014, 03:20

... immunomodulating properties in in vivo mouse model MATERIAL AND METHODS Survival of Lactobacillus strains at low pH and in the presence of bile salt Lactobacillus strains isolated from feces of healthy infants ... 2000) Experimental design in vivo Eight-week-old inbred BALB/c mice (n = per group) were used for in vivo experiments Strains selected in in vitro tests were mixed in equal proportions, lyophilized ... Serum and Vaccine Producer Biomed, Cracow, Poland), Salmonella enterica subsp enterica sv Enteritidis (clinical isolate obtained from the Institute of Sea and Tropical Medicine, Gdynia, Poland),...

Ngày tải lên: 07/07/2014, 19:20

... provide rapid in vitro screens for analyzing new therapies and discovering means of achieving selective protection or targeted killing In light of the ease of use of these in vitro and in vivo assays, ... cytarabine in vivo was associated with adverse effects on the CNS, in regard to both cell death and cell division (as indicated by BrdU incorporation) TUNEL staining was elevated in the SVZ, DG, and ... TUNEL staining, doublecortin staining, S-100b staining, and the merged image (a-d) Images taken at -4 µm; (e-h) -2 µm; (i-l) µm; (m-p) µm The congruence between the doublecortin+ staining and the...

Ngày tải lên: 06/08/2014, 18:21