in vitro and in vivo analysis of the cell

... absence of autoregulation of Gal4p in S cerevisiae reduces the burden of maintaining basal Gal4p Thus, the gene duplication, shuttling of the repressor instead of the inducer and the absence of autoregulation ... requirement of Gal4p for the expression of genes with two binding sites is a result of the strong cooperativity during the binding of the transcriptional activator to the two binding site genes in the ... levels (340 nm), the regulated amount of KlGal1p is insufficient to interact with the high levels of KlGal80p in the nucleus, leading to the inhibition of the GAL switch and thereby reducing expression...

Ngày tải lên: 29/03/2014, 09:20

... the understanding of function of these ligaments and injury mechanisms However, the in- vivo behavior of the lateral ankle ligaments during the range of motion has not been well studied Data in ... each testing position of AJC motion There was no bony interference with the path of the ATFL and CFL The position of the AJC during MR scanning was defined as the neutral reference position The length ... ligaments in their sample of 39 cadaveric ankles.[5] They reported the average length of the ATFL and CFL as 24.8 mm and 35.8 mm respectively The invivo length of the ATFL and CFL measured in this...

Ngày tải lên: 20/06/2014, 01:20

... Kruskal-Wallis analysis of variance and correlation) were used for the analysis of the results In this way, the variability in the number of active regions and the specific activity of the enzyme, ... categories of lines were made for the number of active regions and three for the specific activity of the enzyme RESULTS Results of the analysis of 37 lines homozygous for the Amy allele and 19 s lines ... range within ± SE of the mean, and the group of F/F lines which is at least + SE below the mean (U 7.00, P < 0.05), as well as between the groups of lines of both genotypes within ± SE of the mean...

Ngày tải lên: 09/08/2014, 18:21

... acquisition, analysis and interpretation of data of erythrocyte aggregation of the in vitro experiments GP: acquisition, analysis and interpretation of data of erythrocyte deformability of the in vitro ... chromatography Also involved in drafting the manuscript, revising it critically and in giving final approval of the version to be published LU: acquisition, analysis and interpretation of data of laboratory ... medium and cell factors Consequently, its increase may be explained by the rise in fibrinogen and/ or globulin concentration and/ or to the modification of the erythrocyte surface properties In our in...

Ngày tải lên: 10/08/2014, 05:21

... in the design of the study, and wrote the manuscript JHVDT carried out the sectioning, staining, and analysis of the biopsy specimens, and helped to draft the manuscript EHB participated in the ... biopsies’ of the airway wall are obtained by placing the tip of the miniprobe onto the airway wall surface Another advantage of FCFM and its miniprobe is the ability to reach and therefore visualize the ... was inserted through the working channel of the bronchoscope and placed on the main carina of the airways Special care was taken to position the miniprobe perpendicularly to the surface of the...

Ngày tải lên: 12/08/2014, 13:22

... that controls the protein expression in the wild-type is the shuttling of Gal80p Increasing the amount of Gal80p in the cytoplasm by increasing the distribution coefficient (K) causes the switch ... to the response of a regulatory network for a given input For example, the operation of the switch is constrained by the number of binding sites and the amounts of regulatory proteins Further, ... model provide insights into the importance of their roles in the overall performance of the network A similar experimental evaluation of the roles of individual elementary mechanisms in vivo is very...

Ngày tải lên: 07/03/2014, 16:20

... turnover of airway dendritic cells is in uenced by the in ammatory state of the lungs [24] and that these cells play an essential role both in the induction and maintenance of allergen-driven eosinophilic ... mimicking inhalation of the natural allergen The fact that i.t instillation of [75Se]Der p led to deposition of radioactivity in alveoli and bronchioli with no detectable allergen remaining in the ... that the clearance and further metabolism of the allergen was altered as a result of the in ammation in the lungs of sensitized animals Up to now there are few data available on the fate of an...

Ngày tải lên: 07/03/2014, 21:20

... contains a redox cofactor binding site for either pyrroloquinoline quinone (PQQ) or NADPH In particular, AASDH, containing a PQQ binding domain, is supposed to be involved in lysine degradation and ... absence of the Asp235 can be explained in the light of the model of the interaction of the substrate with the active site (vide infra) It should be noted that the specificity code for the A domains ... for the binding of l-a-aminoadipate to the adenylation domain of the ACV synthetase from Penicillium chrysogenum [7] The results of the docking experiments indicated (Fig 4) that the possible binding...

Ngày tải lên: 23/03/2014, 13:20

... and slides were counterstained with a standard mixture of hematoxylin and eosin [4] Quantitative analysis was done by counting the total number of endothelial cells, identified by lectin staining ... http://www.translational-medicine.com/content/7/1/41 QK performing ex vivo experiments of vascular reactivity in WKY common carotid rings [12], and then we evaluated in vivo the role of this small peptide studying the ... tyrosine kinase (Flt-1) and VEGFR-2 or fetal liver kinase (Flk-1) also known as kinase-insert domain-containing receptor (KDR) [2] In animal models of chronic ischemia, manoeuvres that increase...

Ngày tải lên: 18/06/2014, 15:20

... (Ca+2) and in control (Ctrl)+2) ions the presence of Thickness of actin bundles magnesium (Mg and in Thickness of actin bundles in control (Ctrl) and in the presence of calcium (Ca+2) and magnesium ... after the beginning of incubation Along with the arrest of movement, the addition of the calcium ionophore accelerated and intensified changes in the AC organization produced by EGTA (cf Fig and ... Characteristics of the transgenic tobacco line The expression of plastin-GFP did not affect the responses of chloroplasts in Nicotiana tabacum The amplitudes and kinetics of these responses were about the...

Ngày tải lên: 12/08/2014, 03:20

... expression of TIP3;1 and TIP1;1 in Arabidopsis and found minimal overlap in the timing of their expression, with TIP3;1 being abundant in embryos of mature seeds and sharply declining during seed germination, ... (panels D and E) The onset of fluorescence occurs in different cell types depending on the isoforms TIP1;1-YFP is initially visible in endodermal cells, before extending to every cell type in the differentiation ... z-stacks of the same root, separated by a black line B and C: xz projections of the image stack in panel a, revealing two cross-sections of the root axis, taken in the regions of the root indicated...

Ngày tải lên: 12/08/2014, 03:21

... harvesting the cells and analysis by flow cytometry of the percentage of eGFP-positive cells as well as the intensity of eGFP activity, as determined by the cytometry software Collection and cultivation ... greater than 95% of the cells contained the Y chromosome, indicating that repopulation of the hematopoietic system in the female recipient mice was due to the implantation of the male BMC Hematologic ... from the drug’s effect, yet removal of the drug would result in the reduction in vector expression The insertion of the retroviral vector may induce the unwanted expression of a neighboring oncogene...

Ngày tải lên: 12/08/2014, 04:21

... achieved In this state of absence of information and of wide ignorance of the field, the calculator might have the merit of placing the issue of treatment dose among the priorities of critical ... different modalities and machine settings depending on the preference of the prescribing physician, but a final spKt/V of 1.4 had to be prescribed by means of the Adequacy Calculator The plasma filtration ... measurable and, representing the end of protein metabolism, its accumulation during kidney failure defines the requirement for dialysis while its elimination defines the efficiency of treatment...

Ngày tải lên: 12/08/2014, 22:21

... metalloproteinase (MMP)-9 [16] are overexpressed in the infiltrating mononuclear cells in the patients' spinal cords In addition, a previous report from the United States suggested that the level of HTLV-1 ... essential for the maintenance of leukemic cells in vivo Recently, a novel basic leucine zipper protein encoded by the complementary strand of the HTLV-1 genome, named HTLV-1 basic leucine zipper ... tax per cell (for HTLV-1 infected cell lines) or PBMC (for HAM/TSP, ATL and HCs) (Table 1) Therefore, the HTLV-1 proviral load represents the population of infected cells in PBMCs when one cell...

Ngày tải lên: 12/08/2014, 23:20

... recognize these elements in vitro and in vivo Based on in vivo mutational analyses, two tandem ISL1 binding sites iv are required for enhancer activity in the pituitary and spine and a PITX1 binding ... of the LHX3 gene are necessary for the proper expression of the protein in both the developing pituitary and spinal cord, and that mutations in these elements can result in CPHD 17 This hypothesis ... factors in the diencephalon and oral ectoderm result in the invagination of the oral ectoderm to form Rathke’s pouch, the primordium of the anterior pituitary lobe (Figure 1.1) The first step in the...

Ngày tải lên: 24/08/2014, 11:06

... bespeak the soft civilizing influence, the strain of the primitive, which the Northland had aroused in him, remained alive and active (39) Faithfulness and devotion, things born of fire and roof, ... comparison of the translation with the original An evaluation of the translation: in the translation’s terms and in the critics’ terms An assessment of the likely place of the translation in the target ... transitional role of the interlocutors in the chapter There is not much interaction among characters in the chapter whereas the voice and tone of the writer occupies most of the space in the chapter...

Ngày tải lên: 02/03/2015, 14:26

... century, gaining widespread recognition subsequent to the publication of the novel The call of the wild” in 1903 I.2 The novel The call of the wild” The call of the wild” is the story of Buck, ... together in the TT The purpose of the translator is obviously to enable the TT readers fully understand the exact meaning of the words used in the ST In some cases, the verbs are also inserted into ... writing style in the novel With such a limit set out at the beginning of the study, all the analysis and comparison between the ST and the TT is only restricted in this chapter IV Method of the...

Ngày tải lên: 10/08/2015, 19:50

... is the main focus of the present study and will be discussed in detail later Intra-tumoral injection of DCs offers the theoretical advantage of in vivo loading and activation of DCs which should ... effect of PDT and tumor hyperthermia was consistent with the findings demonstrated by 29 Leunig et al., 1994 in their study evaluating the effect of PDT-induced heating of melanoma in vivo Subsequently, ... studies, the DCs were expanded in vitro and primed in vivo via intratumoral injection of DCs In the present study, the anti-tumor effectiveness of a novel anti-cancer treatment via photoimmunotherapy...

Ngày tải lên: 28/11/2015, 13:45

... started and ceased smoking Data handling and statistical analysis The weighting factors for each record were computed by the ABS to reflect the population at the time of the survey, taking into ... Differences in social factors, education and income explained the increased risk in intermediate transport and production workers but not the protective effect of the professional and some clerical and ... report their height in cm and weight in kg The respondents were asked about LTPA in the prior two weeks including number of times spent in exercise in three categories: walking, moderate and vigorous...

Ngày tải lên: 20/06/2014, 00:20