neural stem cells methods and protocols

... 2002 199. Liposome Methods and Protocols, edited by Subhash C. Basu and Manju Basu, 2002 198. Neural Stem Cells: Methods and Protocols, edited by Tanja Zigova, Paul R. Sanberg, and Juan R. Sanchez-Ramos, ... select against non -neural cells, the percentage of neural progenitors is greatly increased (9). 3 From: Methods in Molecular Biology, vol. 198: Neural Stem...

Ngày tải lên: 11/04/2014, 09:58

... until used and discard after 14 d. Murine Embryonic Stem Cells 3 HUMANA PRESS HUMANA PRESS Methods in Molecular Biology TM Methods in Molecular Biology TM Embryonic Stem Cells Methods and Protocols Edited ... Michael A. Burg, and Andrew Baird 393 29 Single-Cell PCR Methods for Studying Stem Cells and Progenitors Jane E. Aubin, Fina Liu, and G. Antonio Candelier...

Ngày tải lên: 08/04/2014, 12:51

... history and the most advanced discoveries in neural stem cells. This book provides the strategies and challenges of utilizing neural stem cells for therapy of neurological disorders and brain and ... Neural Stem Cells and Therapy, Edited by Tao Sun p. cm. ISBN 978-953-307-958-5 Neural Stem Cells and Therapy 18 Fig. 2. Schematic representation of...

Ngày tải lên: 30/03/2014, 23:20

... Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA ... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA and...

Ngày tải lên: 08/10/2012, 10:25

... system and the anal glands. c. Columnar cells elaborating abundant mucin, e.g., gastric foveolar epithelium and endocervical epithelium. d. Classical goblet cells, e.g., within intestinal and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties, Function...

Ngày tải lên: 08/10/2012, 10:25

... in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The M...

Ngày tải lên: 08/10/2012, 10:25

... GI Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., ... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method...

Ngày tải lên: 08/10/2012, 10:25

... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... Mucin and Leve...

Ngày tải lên: 08/10/2012, 10:25

... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Human...

Ngày tải lên: 08/10/2012, 10:25