neurogenesis neuroinflammation and therapeutic potential of adult neural stem cells

... considered for adult NSC-based therapy in the CNS, the stimulation of endogenous neural progenitor or stem cells and the transplantation of adult- derived neural progenitor and stem cells [39] Self-renewing ... neuronal cells in the adult brain originate from residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells ... plasticity of the nervous system Adult- derived neural progenitor and stem cells grafted in the brain promote neuroprotection, by an immunomodulatory mechanism Grafted neural progenitor and stem cells...

Ngày tải lên: 08/08/2014, 17:20

... Care and Use Committee of UCI and were in accordance with the guidelines of the National Institutes of Health Page of 15 viruses was confirmed by RT-PCR and restriction/ sequence analysis of the ... important feature of functional anti-Ab antibody is the binding to all species of Ab42 peptide and inhibition of cytotoxic effect of Ab42 oligomers and fibrils on human neuroblastoma SH-SY5Y cells We ... surface of VLP in a repetitive and ordered array Such organization of the epitope may induce T cell-independent B cell activation and production of anti-Ab antibodies of IgM isotype On the other hand,...

Ngày tải lên: 18/06/2014, 22:20

... culturing and activation of dendritic cells (DCs) 64 CD4+ T cell selection and proliferation 65 Molecular biology 66 2.3.1 Design and synthesis of EV71-specific primers and probes 66 2.3.2 Design and ... 2.2.1.2 Regeneration and culture of Rhabdomyosarcoma (RD) cells 63 Storage of RD cells 63 2.2.2 T cell proliferation assay 2.2.2.1 Isolation of peripheral blood mononuclear cells (PBMCs) 64 64 ... PROPHLACTIC AND THERAPEUTIC POTENTIAL OF SYNTHETIC PEPTIDES AGAINST ENTEROVIRUS 71 (EV71) DAMIAN FOO GUANG WEI B.Sc (Hons), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF...

Ngày tải lên: 12/09/2015, 08:19

... the stem state of MSCs [14] and other stem cells [15] LIF also activates and represses osteoblast and osteoclast activities [20] The bipotency of LIF suggests that the cellular environment and ... Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy J Cell Mol Med 2004, 8:301-316 31 Tuan RS, Boland G, Tuli R: Adult mesenchymal stem cells ... rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells Proc Natl Acad Sci USA 2001, 98:7841-7845 Tuli R, Tuli S, Nandi S, Wang ML, Alexander PG, Haleem-Smith...

Ngày tải lên: 09/08/2014, 10:20

... embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent cells Conferral of ... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF...

Ngày tải lên: 09/09/2015, 18:56

... stromal stem cells J Cell Sci Suppl :63-76 123 Beresford JN 1989;Osteogenic stem cells and the stromal system of bone and marrow Clin Orthop Relat Res (240):270-280 124 Caplan AI 1991;Mesenchymal stem ... 2001;Mesenchymal stem cells Exp Biol Med (Maywood) (6):507-520 115 Aratani Y, Kitagawa Y 1988;Enhanced synthesis and secretion of type IV collagen and entactin during adipose conversion of 3T3-L1 cells and ... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche,...

Ngày tải lên: 11/09/2015, 09:16

... somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have ... blood cells and platelets, while skin stem cells give rise to the various types of skin cells Some recent reports suggest that adult stem cells such as haemopoietic stem cells, neuronal stem cells ... cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated state has its own copy or copies of...

Ngày tải lên: 16/09/2015, 15:55

... current understanding of SRBD is a detailed knowledge of the anatomical connectivity and molecular basis of action for the brainstem and forebrain networks that govern sleep and arousal, and the means ... Associate Professor, Department z of Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, Ohio, U.S.A Eugene C Fletcher, M.D Professor and Director, Division of Pulmonary and Critical ... Johns Hopkins School of Medicine, Baltimore, Maryland, U.S.A Jean-Paul Praud, M.D., Ph.D Professor, Department of Pediatrics and Department of Physiology and Surgery, University of Sherbrooke, Sherbrooke,...

Ngày tải lên: 14/02/2014, 17:20

... cells and lack of selectivity and damage of healthy tissues Drug delivery systems are aimed to minimise the drug degradation, to increase its bioavailability, to target it to specific cells and ... delivery of TERTsiRNA into tumor cells (murine and human) and silence the TERT gene, which is critical for the development and growth of tumours They observed that treatment of different tumour cells ... functionalisation of CNTs is the alternative and extremely promising approach for applications in fields such as that of functional and composite materials and that of biology According to the location of the...

Ngày tải lên: 17/02/2014, 05:20

... frequency of liver disease, suggesting the influence of alcoholism These results correspond to those of Alvarez et al (6) and Van den Brande (15) but contrast with those of Katz et al (17) and Umeki ... the importance of sputum AFB smear/ culture for the diagnosis of TB in the elderly A positive sputum AFB smear was obtained in 57.1% of elderly and in 57.5 % of young TB patients, and a positive ... limit), Fernandez-Villar et al (30) found an 18.2% incidence of hepatotoxicity in the risk factor group (advanced age, chronic liver disease, abuse of alcohol, and others), and van den Brande et...

Ngày tải lên: 15/03/2014, 03:20

... Siegfried, and Thaddeus G Golos 13 Reproductive Stem Cells of Embryonic Origin: Comparative Properties and Potential Benefits of Human Embryonic Stem Cells and Wharton’s Jelly Stem Cells 136 Chui-Yee ... differentiation of embryonic stem cells into ovarian structures containing oocytes Stem Cells 2006; 24:266–273 24 Qing T, Shi Y, Qin H, et al Induction of oocyte-like cells from mouse embryonic stem cells ... influence of testosterone and supported by both Sertoli and Leydig cells, prospermatogonia enter rounds of mitosis, generating spermatogonial stem cells From within this reservoir of self-generating stem...

Ngày tải lên: 22/03/2014, 20:21

... were used to observe, record, and analyze animal behaviour in terms of signs and extents of muscular atrophy of lips, blink reflex, and ear motion of animals before and 12 weeks after peripheral ... immunohistochemical staining of BrdU and S-100 Representative images of immunohistochemical staining of BrdU and S-100 A: Localization of darkly brownish stained BrdU+ cells to transected tissues of rabbits having ... and scaffold light microscopy of NSC in implanted tex of E16 Sprague-Dawley rat embryos culture on of neural stem cell (NSC) at passage three derived from the composite Representative image of...

Ngày tải lên: 18/06/2014, 15:20

... unrelated to embryonic stem cells Adult stem cells are undifferentiated cells occurring in tissues and organs of adult individuals which are able to convert into differentiated cells of the tissue where ... induced pluripotent stem cells are at least, if not more capable than those from adult (stem) cells Mesenchymal stem cells Although adult stem cells are primarily unipotent cells, under certain ... repair of myocardial infarction, and in stroke [31,32] Other types of stem cells Hematopoietic stem cells together with mesenchymal stem cells, the so-called “side population”, and multipotent adult...

Ngày tải lên: 18/06/2014, 16:20

... caused by loss of or ectopic function of the HH-GLI signaling pathway, and the possible agents that could, in principle, be used as therapeutics Disease type Potential therapeutic Gain -of- function: ... oncogenic mutations in SMOH and PTCH1 or in any element of the pathway that results in activation of GLI function, requires the use of pathway antagonists Up to now, inhibition of ectopic activity has ... from mediating the HH signal [31] (Figure 1) Therapeutic use of antiSHH antibodies is limited to diseases characterized by misexpression of the ligand and cannot generally be applied to tumors,...

Ngày tải lên: 06/08/2014, 18:20

... inflammatory cells, indicated the presence of foci of inflammatory cells and corresponded to diffuse inflammation The sum of steatosis and inflammation scores was used in the results Persons unaware of ... animals and their care were carried out according to the Guidelines for the Care and Use of Laboratory Animals of Seoul National University, Korea Materials The herbal extract mixture was composed of ... the therapeutic effects of the herbal extract mixture and yogurt, the animals were treated for an additional three weeks with the triple administration of ethanol, the herbal extract mixture and...

Ngày tải lên: 07/08/2014, 20:23

... beginning of the steep increase of Fo (Fig 1), [4] 2.3 Seasonal time course of thermostability of photochemistry During 2000, Tc was recorded four times (beginning of April, beginning of May, end of ... (beginning of April, beginning of May, end of August and end of October, i.e., days of year 109, 138, 241, and 297) on needles of potted seedlings of C sempervirens, C atlantica, P halepensis et P ... mixture of sand/peat/soil (1/2/3, v/v/v) and grown in a greenhouse at Avignon, Southern-France, under approx 85% of incident irradiance They were watered once or twice a week with a 1% solution of...

Ngày tải lên: 08/08/2014, 01:22

... schizophrenics) and 67–75% among outpatients, with 45 and 28% of combination therapies in in- and outpatients being reported, respectively [28-30] The rates of prescription of conventional and atypical ... observed: benzodiazepines (69% of FPA patients; 69% of non-FPA patients), atypical (in 51 and 53% of patients respectively) and conventional antipsychotics (in 38 and 46% of patients respectively) ... enrolment, and interpretation of analyzed data and to the review of this paper SF, AR, MS are current employees of Eli Lilly Italy, the study sponsor They contributed to the interpretation of analyzed...

Ngày tải lên: 08/08/2014, 23:20

... naive fraction of CD4+ cells to expand and to become contact-dependent ‘professional’ regulatory T cells TGF-β also induces CD4+ and CD8+ cells that are CD25– to become Th3-like cells Although ... IL-2-activated T cells These effects include upregulation of CD25, CTLA-4 and CD40 ligand expression on CD4+ cells [14,47], and increased tumor necrosis factor-α production by both CD4+ and CD8+ cells [47] ... combination of IL-2 produced by CD4+ cells and the active form of TGF-β produced by natural killer (NK) cells or macrophages (not shown) induce CD8+ cells to lose their cytotoxic potential and become...

Ngày tải lên: 09/08/2014, 03:24

... flattened cells on the surface and round cells in the centre (Fig 3) Spindle-shaped cells were found in the central part of the pellet in some donors, and the frequency of spindleshaped cells was ... stabilizes pH and osmolarity and supplements the cells with trace compounds and energy sources (pyruvate and glucose) The added serum contains high levels of growth factors released by cells and platelets ... to 10 ng of RNA, 15 µl of TaqMan Universal PCR master mixture (Applied Biosystems), 1× assayon-demand mixes of primers and TaqMan MGB probes All samples were analysed in triplicate and PCR was...

Ngày tải lên: 09/08/2014, 06:22