hostpathogen interactions methods and protocols

... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties, Functions, and Applications ... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and Practice (45) provide comprehensive...

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... in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: The Mucins ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ... 1 and 2 (2,3) of this volume for the preparation of secreted and membrane-associated mucins, respectively, and to Chapter 7 (4) for a discussion of methods for mucin separation. 50 McGuckin and...

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... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., Totowa, NJ Separation and Identification ... other identified mucins. Separation and Identification of Mucin 77 7 Separation and Identification of Mucins and Their Glycoforms David J. Thornton, Nagma Khan, and John K. Sheehan 1. Introduction This...

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... values for ρ and η rel. are from ref. 14. Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The ... variety of different sizes and should be chosen according to sample volume and concentration. Heterogeneity and Size Distribution of Gel-Forming Mucins 91 mucins on these grids; and the addition of ... K. E., and Chapman J. A. (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing. J. Ultrastruct. Res. 91, 66–76. 14. Kawahara K and Tanford C. (1966) Viscosity and density...

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... 129 129 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc., Totowa, NJ 12 Synthetic Peptides for the Analysis and Preparation of ... monoclonal antibodies (mAbs) and fragments have been produced by various methods. Some of these have found applications for radio- immunoscintigraphy and targeted therapy of cancer, and others have been ... However, before considering such ambitions, we must first gain an understanding of the molecular interactions between epitope and paratope when an antibody binds to its antigen. It is essential that...

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... lysates and media. Nonspecific bands may be present and can be eliminated from consideration using the pSVL vector control lanes for comparison (Fig. 2A,B, lanes 1 and 3). The sizes of specific bands reported ... polymerase, and incubate the reaction at 72˚C to allow extension of the linked templates. Add 2 µg each of primers 5 and 6, and continue the PCR with 30 cycles at 94˚C for 30 s, 50˚C for 1 min and 72˚C ... 195–205. 12. Bhargava, A. K., Woitach, J. T., Davidson, E. A., and Bhavanandan, V. P. (1990) Cloning and cDNA sequence of a bovine submaxillary gland mucin-like protein containing two distinct domains....

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... or three at the most) and the facilities that are available in the laboratory environment will define From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited ... carbons 4 and 5 and also the exocyclic chain of sialic acid when it is not 8 or 9 substituted. The oligosaccharide-alditol is cleaved in two fragments, one corresponding to the 3-branch and the ... was introduced in the 1970s and rapidly received a large application for analyzing the sequence of N-acetyllactosamine- and oligomannosidic-type glycans. Originally, and before the development...

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... hygroscopic and must be thoroughly dried before weighing and making up as standards. Standards of 1 mM each of 2-, 3-, and 6-sulfo-N- acetylglucosamine in water may be aliquoted and stored at –20°C, and ... equipment and the hydrolysis method are similar for both methods. Although this method is not particularly sensitive by today’s analytical standards ( >1 nmol), it provides fast and reproducible ... sulfate for highly sulfated biomolecules such as mucins. From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc.,...

Ngày tải lên: 08/10/2012, 10:25

Ngày tải lên: 08/10/2012, 10:25

Ngày tải lên: 08/10/2012, 10:25