... test and P < 0.05 wasconsidered to indicate statistical significance.Results Stem- Kine mobilizes CD34 and CD133 Cells Quantification of peripheral blood cells expressing the hematopoietic stem ... infarction [59,60] and stroke [61],are associated with upregulated levels of these cells. Given the possibility that both hematopoieticstem cells and EPC may serve as endogenous “repair cells ,wesought ... feasibility ofsignificant mobilization of cells expressing hematopoietic stem cell and EPC markersand properties. The area ofnutritional modulation of the stem cell compartmentoffers significant...
... 94°C for 2 min, followed by 30 to 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for Cx45, at 56°C for Cx40, and at 57°C for Cx43 for 30 s, and extension at 72°C for 30 s (1 min for ... Michael A. Burg, and Andrew Baird 39329 Single-Cell PCR Methods for Studying StemCellsand ProgenitorsJane E. Aubin, Fina Liu, and G. Antonio Candeliere 40330 Nonradioactive Labeling and Detection ... Troy and Kursad Turksen 25521 Embryonic StemCells as a Model for Studying MelanocyteDevelopmentToshiyuki Yamane, Shin-Ichi Hayashi, and Takahiro Kunisada 26122 Using Progenitor Cells and...
... each fl ask for 3 d. 12. Change the same medium for expanding the cells or use the serum-free medium (DMEM/F12 1Ϻ1) for selecting neural precursor cellsfor another 3 d. 13. Transfer cells from ... count cells, and then plate approx 5 ì 104 cells/ cm2.3.1.4. Culturing of Rat Cells as Monolayers (15–17) 1. Resuspend cells in N2 medium containing 10% FBS and plate at least1 × 104 cells/ cm2 ... Characterization and Utilization of CNS Stem Cells (Gage F. H., and Christen, Y., eds.), Foundation IPSEN, Springer, Heidelberg, pp. 129–149. 8. McKay, R. (1997) Stemcells in the central nervous system....
... of stemcells in repair of liver injuries. Several types of stem cells, such as embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, haematopoietic stem cells, and mesenchymal stem ... cell formation from stem cells; (4) Liver stemcellsand hepatocarcino-genesis; and (5) Application of liver stemcellsfor cell therapy. All these current topics shed light on stem cell technology ... epithelial cells upon 1. Introduction 16 Y.Y. DanESCs and can generate autologous cellsfor individualized treatment. On the other hand, adult somatic stem cells, such as bone marrow MSCs and...
... mechanismresponsible for induction of apoptosis and the relevantsignaling pathways in hematopoieticstemcells are stillpoorly understood. Hematopoieticstemcells (HSCs)contain specific markers on ... iso-lated HSCs/CD34+ cells were harvested for phosphopro-teomic analysis and cultivation of erythroid cells. Thecultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... collected for identi-fication of erythroid markersand cell morphology. Cellsurface membrane markers were analyzed to confirm celltypes of HSCs and erythroid cells. Cell surface markers were...
... mechanismresponsible for induction of apoptosis and the relevantsignaling pathways in hematopoieticstemcells are stillpoorly understood. Hematopoieticstemcells (HSCs)contain specific markers on ... for 4 and 7 days, cells were collected for identi-fication of erythroid markersand cell morphology. Cellsurface membrane markers were analyzed to confirm celltypes of HSCs and erythroid cells. ... iso-lated HSCs/CD34+ cells were harvested for phosphopro-teomic analysis and cultivation of erythroid cells. Thecultivation of erythroid cells derived from HSCs/CD34+ cells was performed according...
... Dental Stem Cells: An Alternative Adult Derived Stem Cell Source for Regenerative Medicine 451 Tammy Laberge and Herman S. Cheung Chapter 24 Pluripotent StemCells from Testis 473 Sandeep ... tremendous variety of approaches and techniques, and this book, Embryonic StemCells - Differentiation and Pluripotent Alternatives and its companion, Embryonic StemCells - Basic Biology to Bioengineering, ... long-term reconstituting hematopoieticstem cells. Genes Dev, 2006. 20(9): p. 1175-86. [152] Katsumoto, T., et al., MOZ is essential for maintenance of hematopoieticstem cells. Genes Dev, 2006....
... of pluripotent stem cells. These include very small embryonic/epiblast like stem cells, multipotent dental stem cells, pluripotent stemcells from testis and amniotic fluid stem cells. In the ... al., An expanded Oct4 interaction network: implications forstem cell biology, development, and disease. Cell Stem Cell, 2010. 6(4): p. 382-95. Embryonic StemCells – Differentiation and Pluripotent ... Dental Stem Cells: An Alternative Adult Derived Stem Cell Source for Regenerative Medicine 451 Tammy Laberge and Herman S. Cheung Chapter 24 Pluripotent StemCells from Testis 473 Sandeep...