Báo cáo khoa học: Identification, structure and differential expression of novel pleurocidins clustered on the genome of the winter flounder, Pseudopleuronectes americanus (Walbaum) ppt

... differential expression of novel pleurocidins clustered on the genome of the winter flounder, Pseudopleuronectes americanus (Walbaum) Susan E. Douglas, Aleksander Patrzykat, Jennifer Pytyck and Jeffrey ... degeneration of the sequences upstream of the pseudogenes made it impossible to predict the locations of the first introns and exons of...

Ngày tải lên: 31/03/2014, 07:20

... these stages in the hydrother- mal vent environment and, second, the aposymbiotic- specific expression condition could be masked by the developmental condition. Comparison with western blots and ... and SDS ⁄ PAGE on cytosolic fractions [14,15] concluded that there were two CA proteins: one of 27 kDa in the branchial plume, and another of 28 kDa in the trophosome. From...

Ngày tải lên: 18/02/2014, 16:20

... loss of the autophophorylation activity (Fig. 1B). The observed changes in the activity of mutants could be due to alterations in the secondary structure of the proteins. The secondary structures ... removes the inhibitory conforma- tion of the activation loop of the kinase, leading to its phosphorylation and further stabilizing the active form of the enz...

Ngày tải lên: 23/03/2014, 06:20

... pCR-h2. Construction of expression vectors. Inserts were isolated from pCR-h1 and pCR-h2 by digestion with Nde 1and BamHI and ligated into the pET11a expression vector linearized with Nde1andBamHI, ... pETHvTrxh1 and pETHvTrxh2, respectively. The sequences of the inserts were determined on both strands and found to be as expected from the identified TC sequences, an...

Ngày tải lên: 23/03/2014, 17:22

... Taken together, the correlation of fully extended structures from Hep III and the proportion of two-PEtn-containing struc- tures with the expression of the Sial-lNnT unit, would suggest that the addition of ... step and (B) one-dimensional TOCSY-NOESY spectrum using selective excitation of H-1 1 H-resonance of the GlcNAc residue in the TOCSY step and of H-3...

Ngày tải lên: 23/03/2014, 21:21

... such G-quadruplex structures in genomic DNA. Materials and methods Preparation, expression and purification of GST fusion proteins The EWS cDNA was cloned into the pGEX6P-1 vector between the EcoRI and XhoI ... the high-affinity Htelo binding of RGG3 and RBD. The ability of RGG3 and RBD to repress transcription activation by EAD raised the possibility that RGG3 an...

Ngày tải lên: 15/02/2014, 01:20

... organization, there is a conservation of synteny between the human and zebrafish genomes where the t-bet and foxp3 genes are found. In contrast, there was no synteny between the human and zebrafish regions containing ... preliminary expres- sion data suggest that these genes are involved in the fish immune response, and that there is a relationship between the expression o...

Ngày tải lên: 16/02/2014, 09:20

... location was determined to be the position of the putative transcription start site (Fig. 1B). Analysis of the 5¢ upstream region of exon 1 demonstrated the lack of a conserved TATA box upstream of ... The pBridge constructs contain two multiple cloning regions and condi- tionally express two proteins: SmSmad4 in-frame with the Gal4BD, and either SmTbRI or SmTbRI-QD...

Ngày tải lên: 18/02/2014, 16:20

... biosynthesis of triterpene saponins is required, including the enzymes catalyzing the sequence of reactions and the genes encoding these enzymes. The biosynthesis of triterpene saponins involves the initial ... range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structural diversity...

Ngày tải lên: 19/02/2014, 07:20

... critical contribution of Utr1p to growth in low iron medium. The contributions of the other two enzymes were also demonstrated; however, these were observed only in the absence of the critical contributor, ... detected on these solid media (data not shown). The expression of POS5, UTR1 and YEF1 via a high-copy vector complemented the poor growth of the pos5 cell...

Ngày tải lên: 20/02/2014, 01:20