Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc

Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc

Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc

... Cloning and characterization of the mammalian-specific nicolin 1 gene ( NICN1 ) encoding a nuclear 24 kDa protein Bianca Backofen 1, *, Ralf Jacob 2, *, Katrin Serth 3 , Achim Gossler 3 , Hassan ... acids and has a calculated molecular weight of 24 kDa (Fig. 1) . The amino acid sequences of the NICN1 proteins are 94% identical between human and...
Ngày tải lên : 23/03/2014, 21:20
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Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx

Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx

... Cloning and characterization of novel snake venom proteins that block smooth muscle contraction Yasuo Yamazaki 1 , Hisashi Koike 1 , Yusuke Sugiyama 1 , Kazuko Motoyoshi 1 , Taeko Wada 1 , Shigeru ... Hishinuma 2 , Mitsuo Mita 2 and Takashi Morita 1 Departments of 1 Biochemistry; and 2 Pharmacodynamics, Meiji Pharmaceutical University, Tokyo, Japan In this study, w...
Ngày tải lên : 18/03/2014, 01:20
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... and XhoI ML2p A- chain 5¢- GATATA CATATG TACGAGCGTCTTCGTCTTCGTGTTACGCATC -3¢ 5¢- CACAC CTCGAG TTATTAAGAAGAAGACGGACGGTCCCGGCATAC -3¢ NdeI and XhoI ML3p A- chain 5¢- GATATA CATATG TACCGTCGTATTAGCCTTCGTGTCACGGAT -3¢ 5¢- CACAC GAATTC TTATTAAGAAGAAGAAGAACGGTCCCTGCATAC -3¢ NdeI and EcoRI ML3.1p A- chain 5¢- GATATA CATATG TACGAGCGTCTTCGTCTTCGTGTTACGCATC -3¢ 5¢- CACAC GAATTC TTATTAAGAAGAAGAA...
Ngày tải lên : 07/03/2014, 15:20
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Báo cáo khoa học: Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf

Báo cáo khoa học: Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf

... (28) 13 .5 (24) 23.0 ( 41) 23.6 (42) 21. 9 (39) 21. 3 (38) Positive charged a (%) 9.9 (18 ) 12 .4 (22) 11 .8 ( 21) 11 .8 ( 21) 11 .8 ( 21) 11 .8 ( 21) Isoelectric point 7. 91 9.83 11 .16 10 . 71 11. 16 11 .16 a Percentage ... underlined and the 13 repeats with a consensus of CCAATGATGATCCAAGAAATCCACACTACAG ( 31 bp) in the 3¢-UTR are numbered and distinguished fro...
Ngày tải lên : 07/03/2014, 16:20
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... forward) 45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ PsCBL (degenerate reverse) 55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward) 65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR reverse) 75¢-CGAGCTCACTGCCTCTCAAC-3¢ ... component of the nucleolus and sti- mulated by phosphorylation with CK2 and cdc2 protein kinases. Plant J 25, 9 17 . 32 Yadav N, Chandok MR, Prasad J, Bhattacharya S, Sopor...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... 5¢-TTCCAAGAGCTGAACAAACCATTG-3¢; HSF1b forward, 5¢-GAAGCAGCTGGTCCAGTACAC CTC-3¢; HSF1b reverse, 5¢-GGCTGAATAAACCATGC CAGTAGC-3¢; HSC70 forward, 5¢-ACATCAGCGACA ACAAGAGG-3¢; HSC70 reverse, 5¢-AGCAGGTCCTG GACATTCTC-3¢. ... PCR was performed with the M13 forward primer (5¢-CCCAGTCACGACGTTGTAAAA CG-3¢)asasenseprimerandHSF1-specific primers (for HSF 1a, 5¢-GAAGCAGCTTGTCCAGTACACTAA-3¢;for HSF1b,5¢-...
Ngày tải lên : 19/02/2014, 12:20
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

... 5¢-GATGGTATTGATTTTGCCATAGAGCATGGTTCA-3¢ Anti-sense strand 5¢-TGAACCATGCTCTATGGCAAAATCAATACCATC-3¢ Asp125Asn Sense strand 5¢-TTGGATGGTATTGATTTTAACATAGAGCATGGTTCAACC-3¢ Anti-sense strand 5¢-GGTTGAACCATGCTCTATGTTAAAATCAATACCATCCAA-3¢ Glu127Ala ... 5¢-GGTTGAACCATGCTCTATGTTAAAATCAATACCATCCAA-3¢ Glu127Ala Sense strand 5¢-GGTATTGATTTTGACATAGCGCTATGTCAAAATCAATACC-3¢ Anti-sense strand 5¢-GTACAGGGTTGAA...
Ngày tải lên : 22/02/2014, 04:20
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Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx

Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx

... and fungi [10 ]. Gene c loning of D14-SR from Ara bidopsis thaliana and analysis o f m utants has h ighlighted the role of the protein in cell growth and embryonic development of the plant [11 ,12 ]. Inherited ... two parts ( Fig. 1) , which accounted for the calculated molecular mass of  46.7 kDa. Therefore, the discrepancy between the appar- ent molecular m...
Ngày tải lên : 08/03/2014, 16:20
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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

... l1 918 5; barley: BAS1, Z34 917 , PER1, X965 51; Arabidopsis: BAS1, X97 910 , TPx2, AF1 213 56, MHF15 .19 , AF3268 71, Per1, O04005; Chlamydomonas Ch-Prx1, AJ304857; spinach B AS1, ´ 94 219 ; mouse TPx, u20 611 ; ... using an intensifying s creen, at )80 °C. RNA for Northern analyses was isolated as described above and separated in a 1. 3% agarose/formaldehyde gel. The RNA was b lotted...
Ngày tải lên : 08/03/2014, 16:20
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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

... K-dependent carboxylase: effect of ammonium sulfate on substrate carboxylation and on inhibition by stereospific substrate analogs. Biochim. Biophys. Acta 10 34, 11 16 . 51. Bandyopadhyay, P.K., Garrett, ... C-terminal extension relative to the mammalian carboxylases. The Conus carboxylase has 811 amino acids compared with 758 amino acids for most of the mammalian carboxylas- es....
Ngày tải lên : 17/03/2014, 10:20
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