Báo cáo khoa học: Identification, cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome pot

... about the origins of HvTrxh1 and HvTrxh2, the occurrence of the thioredoxin h spots in protein extracts from dissected barley seeds was analysed by 2D gel electrophoresis (Fig. 1B). The three thioredoxin ... cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome Kenji Maeda, Chr...

Ngày tải lên: 23/03/2014, 17:22

... question is whether expression of the newly identified SULT1 ST5 correlates with the development of the thyroid hormone endocrine system of the zebra- fish. To gain insight into this, RT-PCR was ... to amplify the complete coding region of this novel zebrafish SULT. With zebrafish total RNA as the template and oligo(dT) as the primer, the first-strand cDNA was syn- thesiz...

Ngày tải lên: 23/03/2014, 15:20

... block the interaction between the EAD and RNA polymer- ase II subunit [11,45]. The interaction between the EAD and RGG3 might inhibit the high-affinity Htelo binding of RGG3. To gain further insight ... S3). The EAD domain therefore inhibited the high-affinity Htelo binding of RGG3 and RBD. The ability of RGG3 and RBD to repress transcription activation by EAD rais...

Ngày tải lên: 15/02/2014, 01:20

... This approach was not used for stat6 as the region in which this gene was found in the zebrafish genome shared no synteny with the human genome. The zebrafish gen- ome was searched using the human ... end of the gene, where the zebrafish and human sequences appear to be quite diverse. Together with the well-conserved gene organization, there is a conservation of synteny bet...

Ngày tải lên: 16/02/2014, 09:20

... genes from other species. For example, the loca- tion of the intron within the MH1-encoding region is conserved in the human Smad4 gene, whereas the intron within the linker-encoding region of SmSmad1B is ... Smad3. The R-Smads of the BMP pathway include Smads 1, 5 and 8. The type I receptor phos- phorylates the R-Smad at its C-terminal MH2 domain, causing the...

Ngày tải lên: 18/02/2014, 16:20

... reach the threshold for the RpCAbr tran- script in the branchial plume compared to the tropho- some whereas approximately five fewer cycles are required to reach the threshold for RpCAtr in the trophosome ... species, and other taxa, from hydrothermal vents and elsewhere. Biol Soc Wash Bull 6, 117–158. 2 Childress JJ & Fischer CR (1992) The biology of hydro- thermal v...

Ngày tải lên: 18/02/2014, 16:20

... identical to that of the authentic olean-3b,24-di- acetoxy-12-ene, except for the presence of several back- ground peaks (the amount of authentic sample was adjusted to equalize the height of both peaks ... and characterization of the Arabidopsis thaliana lupeol synthase gene. Phytochemistry 49, 1905– 1911. 11 Shibuya M, Zhang H, Endo A, Shishikura K, Kushiro T & E...

Ngày tải lên: 19/02/2014, 07:20

... 7). The deletion of YEF1 or POS5 from utr1 further decreased the growth of utr1 to the same level as that of the ftr1 mutant, which lacks a high-affinity iron transporter and shows severe growth defects ... and trimetaphosphate at 5 mm and polyphosphate, metaphosphate and hexametaphos- phate at 1 mgÆmL )1 ) and phosphorylated compounds (phosphocreatine, glucose-6-phos...

Ngày tải lên: 20/02/2014, 01:20

... are distinguished by charge and hydrophobicity pro®le [3±5]. The N- and H -regions are t hought to interact with the translocase machinery and to mediate membrane insertion, whereas the C-region ... 2), and then cloned into the pQE16 vector. A fter restriction e nzyme digestion of p QE derivated plasmids (pQSh, pQTh, p QVh, pQWh, pQBh), the His-tagged genes (sipSh, sipTh...

Ngày tải lên: 21/02/2014, 03:20

... possible that the charge repulsion between the phosphate groups in the mid- region and the one located in the C-terminal portion of the protein may cause a conformational change that makes the cleavage ... increases the susceptibility to cleavage by the amniotic fluid pro- tease and suggest that not only the degree, but also the position of the phosphate groups...

Ngày tải lên: 07/03/2014, 00:20