Electrophoresis
Tài liệu Kỹ thuật điện di ADN - DNA electrophoresis pptx
... dodecyl sulfate) để bọc protein với điện tích âm Kỹ thuật điện di ADN DNA electrophoresis Fig 20-1: DNA separation by gel electrophoresis http://a32.lehman.cuny.edu/molbio_course/agarose.htm So ... một base T hình thành hai liên kết hydro còn giữa G và C hình thành ba liên kết. Khái niệm DNA electrophoresis là quá trình phân tách một hỗn hợp các phân tử DNA nhờ một chất nền...
Ngày tải lên: 22/12/2013, 15:16
Gel electrophoresis
... Practice of Agarose Gel Electrophoresis Experiment Procedures Conducting Agarose Gel Electrophoresis ELECTROPHORESIS SAMPLES Samples in EDVOTEK Series 100 and Sci-On® Series electrophoresis experi- ments ... "workhorse" of agarose gel electrophoresis is the horizontal gel electrophoresis apparatus. There are many types of electrophoresis units, but the horizontal electr...
Ngày tải lên: 23/02/2014, 11:10
Electrophoresis
... CAPILLARY ELECTROPHORESIS 23.4A What Is Capillary Electrophoresis? Another type of electrophoresis is the method of capillary electrophoresis (CE). CE is a technique that separates analytes by electrophoresis ... Band-Broadening 23.3 Gel Electrophoresis 23.3A What Is Gel Electrophoresis? 23.3B How Is Gel Electrophoresis Performed? 23.3C What Are Some Special Types of Gel Elec...
Ngày tải lên: 23/02/2014, 11:12
INTRODUCTION TO ELECTROPHORESIS
... for electrophoresis Temperature and Electrophoresis ◆ Important at every stage of electrophoresis – During Polymerization » Exothermic Reaction » Gel irregularities » Pore size – During Electrophoresis » ... electrical equations are important in electrophoresis – The first is Ohm's Law, I = E/R – The second is P = EI – This can also be expressed as P = I 2 R ◆ In electrophore...
Ngày tải lên: 13/03/2014, 19:24
AAC chap 3 principles of electrophoresis new
... pK1:2.34, pK2, 9.4) 1 Chap 3 Electrophoresis Methods Biochemistry and Molecular Biology 10.1 general principles 10.2 Support media 10.3 Electrophoresis of protein 10.4 Electrophoresis of nucleic ... their ratio. 5 %T 10 %T 15 %T 20 %T 21 Polyacrylamide Gel Electrophoresis (PAGE) Molecular sieving properties of the gel 22 Polyacrylamide Gel Electrophoresis (PAGE) Separation of prot...
Ngày tải lên: 13/03/2014, 19:25
Gel Electrophoresis – Teacher Instructions
... Initiative Gel Electrophoresis – Teacher Instructions Suggested Grade Level: Grades 7-14 Class Time Required: 1 period (50 minutes) EQUIPMENT AND MATERIALS NEEDED (per group) • Electrophoresis ... form and comb • Electrophoresis power supply • Agar gel • Salt solution • Scientific stains to use as samples • Sample-loading device • Masking tape, if needed to seal gel form GEL ELECTROPHO...
Ngày tải lên: 13/03/2014, 19:38
Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx
... Natl Acad. Sci. USA 94, 5090–5094. 19. Scha ¨ gger 1 , H. & von Jagow, G. (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. ... 271) 385 Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobili...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Angiotensin-converting enzyme inhibition studies by natural leech inhibitors by capillary electrophoresis and competition assay doc
... pellet and peptides were analyzed by capillary zonal electrophoresis. Samples (2 nL) were injected under vacuum into a PACE 5000 capillary electrophoresis system (Beckman) equipped with a silica ... presence of selective inhibitor using capillary zonal electrophoresis, several parameters have to be established. Fig. 1 shows the capillary zonal electrophoresis profile of FMRF-amide (inte...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis doc
... dephosphoryla- tion analyzed by phospho-affinity gel electrophoresis. (A) Time course of CK2-dependent Cdc37 phosphorylation was analyzed by phospho-affinity gel electrophoresis, between 0 min (lane 1) and 10 ... polyacrylamide gel electrophoresis Phos-tag acrylamide was purchased from the Phos-Tag Consortium (http://www.phos-tag.com), and 5 mm stock solutions in water were prepared. Phosph...
Ngày tải lên: 30/03/2014, 03:20
Báo cáo Y học: Characterization of heparin binding by a peptide from amyloid P component using capillary electrophoresis, surface plasmon resonance and isothermal titration calorimetry ppt
... heparin-binding peptide from human serum amyloid P component characterized by affinity capillary electrophoresis. Electrophoresis 19, 442–447. Fig. 6. Ribbon structure of serum amyloid protein (SAP) monomer ... associated with protein or peptide heparin binding. Here, we characterize the bind- ing using capillary electrophoresis (CE), surface plasmon resonance (SPR), and isothermal titrati...
Ngày tải lên: 31/03/2014, 23:20
- coupling electrochemical detection with microchip capillary electrophoresis
- microchip capillary electrophoresis systems for dna analysis
- homogenization cytoplasmic protein extraction gel electrophoresis and densitometry analysis
- and two dimensional sds polyacrylamide gel electrophoresis sds page
- 2d gel electrophoresis lc ms ms and western blotting
- t rflp and denaturing gradient gel electrophoresis dgge
- electrophoresis protein visualization and image analysis