coupling electrochemical detection with microchip capillary electrophoresis

... Chen FTA: Rapid protein-analysis by capillary electrophoresis J Chromatogr 1991, 559:445-453 Dolník V: Applications of capillary electrophoresis on microchip Electrophoresis 2006, 28(15):1094-2009 ... fluorescent peptides with capillary zone electrophoresis with laser-induced fluorescence Electrophoresis 2003, 24:892-896 Yang WC, Schmerr MJ, Jackman R, Bodemer W, Yeung ES: Capillary electrophoresis- based ... Schmerr MJ: Detection of prion protein using a capillary electrophoresis- based competitive immunoassay with laser-induced fluorescence detection and cyclodextrin-aided separation Electrophoresis...

Ngày tải lên: 11/08/2014, 00:22

... orientation on the film and to be highly compatible with many biological functions without denaturation [16] However, with such an approach, the electrochemical detection of the immunological recognition ... 0.06 cm2 Electrochemical measurement Electrochemical polymerization and characterization was performed using an AUTOLAB PGSTAT 12 electrochemical analysis system with GPES software The electrochemical ... mM, pH 7.4 3.3 Detection of antigen by electrochemical and SPR methods Antigen–antibody reactions can be followed directly by electrochemical techniques due to the high intrinsic electrochemical...

Ngày tải lên: 13/09/2015, 18:05

... distributions of unbounded size with a dynamic parameter set that grows with the size of the training data One method of TSG induction is to represent a probabilistic TSG with Dirichlet Process priors ... grammaticality detection Tree Substitution Grammars Tree Substitution Grammars are similar to Context Free Grammars, differing in that they allow rewrite rules of arbitrary parse tree structure with any ... parsing, competing closely with the absolute best results set by refinement based parsers 2.3 Fragment Based Classification The use of parse tree fragments for classification began with Collins and Duffy...

Ngày tải lên: 07/03/2014, 18:20

... connection with ZnS, CdS, PbS, and CuS colloidal crystals, respectively [30] The assay shows the efficient coupling of the multiprotein electrical detection with the amplification feature of electrochemical ... Strategies used for DNA detection by labeling with metallic NPs Usually a probe DNA has been immobilised on a transducing platform and then hybridized with target DNA and further with NP-modified DNA ... electrochemical tracers The advantages offered along with the possibility of being used in several biosensing systems based on electrochemical techniques require the development of novel NP-detection...

Ngày tải lên: 16/03/2014, 12:20

... were analyzed by capillary zonal electrophoresis Samples (2 nL) were injected under vacuum into a PACE 5000 capillary electrophoresis system (Beckman) equipped with a silica capillary (length ... cross-reaction with LORF was obtained The specificity of ACE antiserum has been described elsewhere [29] In brief, 20% of cross-reaction with rabbit ACE was observed Fig Capillary zonal electrophoresis ... sectioned at lm After removal of paraffin with toluene, the sections were successively treated either with the anti-(LORF-amide) or with the anti-ACE diluted : 800 and with goat anti-(rabbit IgG) IgG conjugated...

Ngày tải lên: 23/03/2014, 12:20

... capillary temperature thermostatting of 20 °C The capillary was rinsed after electrophoresis for with each of the following: 0.1 M NaOH, water, and electrophoresis buffer Samples were pressure injected ... fused silica capillary with 50 cm to the detector window and of 57 cm total length Separations were carried out at 17 or 18 kV corresponding to a current of approximately 85 lA at a capillary temperature ... peptide) with a constant concentration of LMW heparin Preliminary experiments were carried out with mixtures of the scrambled and the unpurified AP-1 preparation in 10 mM sodium phosphate, pH 7.46 with...

Ngày tải lên: 31/03/2014, 23:20

... The capillary is rinsed and filled with the run electrolyte This allows the capillary to adjust to the pH of the electrolyte A typical rinse time may be with the displaced drops from the capillary ... steps for capillary coatmgs should be avoided unless prescribed in the instructions supplied with the capillary Once the capillary is regenerated, it is not necessary to rehydrate it with an extended ... should be flushed with water and then air to remove electrolyte from the capillary Storage filled with air prevents formation of a gel layer inside the capillary that can form if the capillary is...

Ngày tải lên: 10/04/2014, 11:11

... Carbohydrates 47 Fig Capillary electrophoretic analysis of glucobioses and galactosylglucoses as PMP derivatives by affinity capillary electrophoresis in the presence of RCA60 with UV detection Capillary, ... oligosaccharide by capillary electrophoresis Electrophoresis 19, 2639–2644 Saccharide Diversity 21 53 Denuzière, A., Taverna, M., Ferrier, D., and Domard, A (1997) Capillary electrophoresis of ... (1997) Recent developments in capillary electrophoresis of carbohydrate species Electrophoresis 18, 2400–2407 56 Scapol, L., Marchi, E., and Viscomi, G C (1996) Capillary electrophoresis of heparin...

Ngày tải lên: 10/04/2014, 11:11

... gel electrophoresis CGE, non-denaturing capillary gel electrophoresis CEMSA, capillary electrophoresis mobility shift assay CE/MS, capillary electrophoresis/ mass spectrography Chip capillary electrophoresis ... Mathies, R A (1998) Capillary electrophoresis chips with integrated electrochemical detection Anal Chem 70, 684–688 97 Baba, Y (2001) Capillary affinity gel electrophoresis, in Capillary Electrophoresis ... temperature-programmed capillary electrophoresis Ultra-fast capillary electrophoresis Reference to conventional mutationdetection methods 34,35 30 Reference to analysis using capillary electrophoresis...

Ngày tải lên: 10/04/2014, 11:11

... capillaries A detection window is created in the capillary by burning the polyimide coating with a gentle flame, then washing the area with methanol Each capillary is then rinsed with water for min, with ... 2.1 Capillary Electrophoresis Fast capillary, fused silica (effective length): cm × 50 µm (id), (DB-1, J&W Scientific, CA) Capillary 6.3: 12.1 cm × 50 µm id Constant denaturant capillary electrophoresis ... EDTA, pH 8.8 2.2 Chip Electrophoresis Electrophoresis chip with integrated electrochemical detection Sieving medium: 0.75% (w/v) hydroxyethyl cellulose in 1X TBE buffer, pH 8.8 with µM EtBr DNA:...

Ngày tải lên: 10/04/2014, 11:11

... serum cryoglobulins by capillary zone electrophoresis Electrophoresis 17, 1607–1612 Freidberg, M A and Shihabi, Z K (1997) Urine proteins analysis by capillary electrophoresis Electrophoresis 18, ... laser-induced fluorescence detection in applications of capillary electrophoresis J Cap Electrophor 3, 89–110 11 Oda, R P and Landers, J P (1996) Introduction, in Capillary Electrophoresis (Landers, ... zone electrophoresis and isoelectric focusing in capillary electrophoresis J Chromatogr 480, 311–319 17 Chen, S-M and Wiktorowicz, J E (1992) Isoelectric focusing by free solution capillary electrophoresis...

Ngày tải lên: 10/04/2014, 22:16

... www.it-ebooks.info The time is boring without random occurrence The research is monotonous without incomplete information The life is tedious without fault detection The living is tough without noise filtering ... Detection for Markov Systems with Varying Nonlinearities Problem Formulation for Robust H∞ Filter Design Performance Analysis of Robust H∞ Filter Design of Robust H∞ Filters Fault Detection with ... fault detection problems for nonlinear Markovian jump systems with sensor saturation and RVNs In Chapter 6, the fault detection problem is considered for two classes of discrete-time systems with...

Ngày tải lên: 24/04/2014, 15:12

... Processing function to the pth microphone with the time delay, τkp , factored out, and the summation is only over the K effective sources with path delays falling within interval Δl At this point, whitening ... 16] showed that significantly better detection rates are achieved with partial whitening, rather than total whitening Therefore, partial whitening results with β = 0.75 are also included in the ... set to zero in order to substitute out V p (ω, l) in the expected value of (8) with the expression in (2) and Bip with the expression of (9) Now assuming that distinct noise sources are uncorrelated,...

Ngày tải lên: 21/06/2014, 06:20

... between FDBD with QRM-MLD, MMSE-FDE, and FDISIC in uncoded TA-SC 64QAM is used, FDBD with QRM-MLD can achieve better BER than FDISIC even if M = is used When 16 (64) QAM is used, FDBD with QRM-MLD ... complexity.FDBD with QRM-MLD significantly reduces the complexity compared to the MLD However, the computational complexity of FDBD with QRM-MLD is still much higher than MMSEFDE and MMSE-FDE with iterative ... using MMSE-FDE Turbo decoding with iterations is performed after FDBD with QRM-MLD and also after MMSE-FDE It can be seen form Figure 11 that when R = 3/4 and 8/9, FDBD with QRM-MLD provides much...

Ngày tải lên: 21/06/2014, 08:20

... watermark detection Given a host signal x, a watermark w, and a pair of keys {Kemb , Kdet } for embedding and detection (they are the same key in symmetric schemes), a digital blind watermark detection ... watermark w, and the detection key Kdet , and it outputs a Boolean value indicating whether the signal z contains the watermark w, without using the original host data x Three detection algorithms ... additive spread spectrum with a correlation-based detector (SS), spread-transform dither modulation without distortion compensation (ST-DM), and additive spread spectrum with a generalized Gaussian...

Ngày tải lên: 21/06/2014, 22:20

... the blink detection systems feasible However, for videos with large variations, such as the typical videos collected from in-car cameras, the acquired images are usually noisy and with low-resolution ... error in pixels after normalization with the scales shows the error in pixels after normalization with the scales The error is the distance between the center of detection to the groundtruth Experimental ... closed eyes Eye detection algorithms can be used to give the initial position of the eyes [10–12], and after that the interactive particle filters are used for eye tracking and blink detection The...

Ngày tải lên: 22/06/2014, 19:20

... Analytical Chemistry Capillary Zone electrophoresis (CZE) Capillary gel electrophoresis (CGE) Capillary electrochromatography (CEC) Capillary isoelectric focusing (CIEF) Capillary isotachophoresis ... – Conductometric detection: like IC J Tanyanyiwa, S Leuthardt, P C Hauser, Conductimetric and potentiometric detection in conventional and microchip capillary electrophoresis, Electrophoresis ... delfin.klte.hu/~agaspar/ce-research.html Capillary Gel Electrophoresis (CGE) Capillary Gel Electrophoresis (CGE) is the adaptation of traditional gel electrophoresis into the capillary using polymers in solution...

Ngày tải lên: 27/06/2014, 01:20

... namely capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), isotachophoresis (ITP), isoelectric focusing (IEF), capillary electrokinetic chromatography (EKC) and capillary ... deals with the theory and practice of the advanced chiral analysis of biologically active substances, beginning with the chiral separation, continuing with sample preparation and finishing with detection ... mediated micellar electrokinetic chromatography, NACE = non-aqueous capillary electrophoresis, MCE = electrophoresis on microchip, CEC = capillary electrochromatography, S-CD = sulphated-CD, S-CD...

Ngày tải lên: 28/06/2014, 06:20

... label-free and reagentless electrochemical DNA sensing based on SWNTs and an immobilized redox probe This system allows very specific detection of DNA but the limit of detection is only 0.1 mM 3D ... weak whereas peak ♯1 becomes predominant, along with a new shoulder (♯3) at −0.32 V Peak ♯3 increases with the o-MWCNT density (data not shown) 3.3 Detection of miRNA ODN probes (ODN-141-P) were ... direct electrochemical detection of miR141, a miRNA biomarker It is shown that the copolymer electroactivity is enhanced by the presence of o-MWCNTs, which probably participate to the low detection...

Ngày tải lên: 02/07/2014, 14:14