microchip capillary electrophoresis systems for dna analysis

... therefore has great potential for on-site analysis of various pollutants at trace level CE is found to be a versatile analytical tool for the analysis of DNA as well as pollutants Combination of DNA ... 3.14 Electrophoresis of ΦX174 DNA in Buffers containing Citrate or GNPs-a 101 Figure 3.15 Electrophoresis of 2-Log DNA Ladder by CE-UV 102 Figure 3.16 Electrophoresis of ΦX174 DNA ... based on Capillary Gel used for analysis of differences in solute size as DNA molecules and Electrophoresis analytes migrate through the (CGE) SDS-denatured pores of the gel-filled capillary...

Ngày tải lên: 14/09/2015, 11:40

... C M Silvestre, “Performance analysis of robust audio hashing,” IEEE Transactions on Information Forensics and Security, vol 2, no 2, pp 254–266, 2007 EURASIP Journal on Information Security ... between the initial pair of bits is zero 6 EURASIP Journal on Information Security Before proceeding, note that the transition matrix for the elemental distance process is A = Π2 and, from (30), ... distance Markov chain approximation for 4-ary hashes The CLT approximation has good agreement in the binary case for n > 20, but is significantly less accurate for 4ary hashes This is due to the...

Ngày tải lên: 22/06/2014, 06:20

... XII CHAPTER Electrophoresis of Small Biomolecules 1.1 Principles of capillary electrophoresis 1.2 Microchip capillary electrophoresis 1.3 Analysis of small biomolecules ... spectrometry CE: Capillary electrophoresis HPLC: High performance liquid CGE: Capillary gel electrophoresis chromatography CIEF: Capillary isoelectric focusing HPLC-MS: High performance liquid ... 1-butyl-3- DNA: Deoxyribonucleic acid methylimidazolium DNase: Deoxyribonuclease µ-CAE: Micro -capillary array dsDNA: Double stranded electrophoresis deoxyribonucleic acid µ-TAS: Micro-total analysis...

Ngày tải lên: 02/10/2015, 12:56

... (as in gel electrophoresis) or can be a liquid, in most cases a buffer solution In capillary electrophoresis, the medium is contained in a small capillary 40 Therefore capillary electrophoresis ... diagram of capillary electrophoresis system High performance capillary electrophoresis (HPCE) does not require the use of gels because the capillary walls provide mechanical support for the carrier ... of EOF profile 24 1.4.3 Detection in Capillary Electrophoresis Conventional high performance capillary electrophoresis (HPCE) was developed based on high performance liquid chromatography (HPLC)...

Ngày tải lên: 11/09/2015, 10:14

... Cyclodextrin CE Capillary Electrophoresis CEC Capillary Electrochromatography CF Concentration factor CF-FAB Continuous-flow fast atom bombardment CGE Capillary gel electrophoresis CIEF Capillary isoelectric ... same portable capillary electrophoresis system as in the previous chapter, and potentially can be used for field analysis of chiral compounds In addition, a fast method for the analysis of underivertized ... FMOC 9-fluorenylmethyl chloroformate GC Gas chromatography GC-MS Gas chromatography–mass spectrometry HPCE High performance capillary electrophoresis HPLC High performance liquid chromatography...

Ngày tải lên: 14/09/2015, 10:36

... are urgent needs for analytical systems for homeland security Methods for fast analysis of stabilizers in gunpowder and propellants were developed on our portable capillary electrophoresis system ... Landers, Handbook of Capillary Electrophoresis, USA, 1996 [5] R Kuhn, S H Kuhn, Capillary Electrophoresis: Principles and Practice, Berlin, 1993 [6] P G Righetti, Capillary Electrophoresis in Analytical ... Schmitt, Capillary Electrophoresis Method and Potentials, Lengerich, 1994 [2] D J Holme, H Peck, Analytical Biochemistry, Longman, 1998 [3] B Chankvetadze, Capillary Electrophoresis in Chiral Analysis, ...

Ngày tải lên: 14/09/2015, 10:37

... 1.1 Electrophoresis 1.2 History of capillary electrophoresis 1.3 Basic principles of capillary electrophoresis 1.4 Different modes of capillary electrophoresis 19 1.5 Instrumentation for capillary ... capillary electrophoresis 24 1.6 Scope of research 32 Part I 35 Analysis of intact bacteria and bacterial DNA Chapter Electrophoretic behavior analysis of intact bacteria by capillary electrophoresis ... technique for the separation of double-stranded DNA, single-stranded DNA and polymerase chain reaction products Initially, CGE was carried out by converting gel electrophoresis of slab format to capillary...

Ngày tải lên: 15/09/2015, 17:11

... History of Capillary Electrophoresis Electrophoresis is a liquid phase separation technique based on ionic mobility differences of species in an electric field Capillary electrophoresis (CE) is electrophoresis ... HPLC, the limits of detection (LOD) for capillary electrophoresis are constrained by the dimensions of the capillary For example, the small volume of the capillary limits the total volume of ... open-tubular preconcentrator for CE In this approach the wall of a 20-cm capillary was modified with a C18 phase for herbicide analysis, or a metal chelate phase for 16 protein analysis The diluted analytes...

Ngày tải lên: 16/09/2015, 15:55

... method for bound constrained gloabal optimization J Glob Optim 39, 197–219 34 Schmidt H & Jirstrand M (2006) Systems Biology Toolbox for MATLAB: a computational platform for research in systems ... with the software packages systems biology toolbox2 and the sbpd Extension Package as described in [34] Both matlab and systems biology toolbox2 are necessary for the performance of model simulations ... infrared gas analysis Measurements were performed during the first h 508 of the light phase, representing the time period of photosynthetic activity until plants were harvested for analysis of...

Ngày tải lên: 14/02/2014, 22:20

... Methods for Stability Analysis of Electric Power Systems ffirs.indd i 9/24/2010 2:20:04 PM ffirs.indd ii 9/24/2010 2:20:04 PM Direct Methods for Stability Analysis of Electric Power Systems Theoretical ... significant research efforts, for example, those documented in Choi (2006), CIGRE Task Force 38.02.05 (1990), He et al (2006), IEEE Task Force on Load Representation for Dynamic Performance (1993), ... computer program power system analysis These static load models are adequate for some types of power system dynamic analysis but not for others There remains a necessity for the development of accurate...

Ngày tải lên: 30/05/2014, 23:38

... over intact control specimens for both cable-plate and screw-plate systems for all constructs considered Screw-plate systems were stiffer than cable-plate systems for Constructs A and B (p < 0.046), ... constructs Each of the cable-plate systems (constructs A, B, and C) were mechanically tested and subsequently transformed into screw-plate systems for retesting For the cable-plate setup, construct ... over intact control specimens for both cable-plate and screw-plate systems for all constructs considered Cable-plate and screw-plate systems were equally stiff for a given construct A, B, or...

Ngày tải lên: 20/06/2014, 04:20

... namely capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), isotachophoresis (ITP), isoelectric focusing (IEF), capillary electrokinetic chromatography (EKC) and capillary ... Microchip capillary electrophoresis Microfluidic devices, such as microchips (Figure 2.15), can provide several additional advantages over electromigration techniques performed in capillary format ... mediated micellar electrokinetic chromatography, NACE = non-aqueous capillary electrophoresis, MCE = electrophoresis on microchip, CEC = capillary electrochromatography, S-CD = sulphated-CD, S-CD...

Ngày tải lên: 28/06/2014, 06:20

... 742:257-266 Chen FTA: Rapid protein -analysis by capillary electrophoresis J Chromatogr 1991, 559:445-453 Dolník V: Applications of capillary electrophoresis on microchip Electrophoresis 2006, 28(15):1094-2009 ... used was 50 mM borate, pH 9.2 Gravity injection performed by elevating inlet capillary cm for s Applied voltage for CE separation was 20 kV Capillary temperature maintained at 20°C Excitation ... used for conjugation was rabbit anti-human albumin CE buffer electrolyte used was 50 mM borate, pH 9.2 Gravity injection performed by elevating inlet capillary cm for s Applied voltage for CE...

Ngày tải lên: 11/08/2014, 00:22

... HSA-gene HSA-gene Figure format of the vectors used in the study Linearized Linearized format of the vectors used in the study G1: pcDNA3.1/GS-HSA, H1: pcDNA3.1/V5-His+HSA, H2: pcDNA3.1/V5-His+HSA with ... Lipofectamine 2000, cultured for 48 h, harvested and applied to Western blot Control (C) cells treated the same way without DNA (A) HSA vectors: pcDNA3.1/GS (G1), pcDNA3.1/V5-His (H1), pcDNA3.1/V5-His with ... Lipofectamine 2000, cultured for 48 h, harvested and applied to Western blot Control (C) cells treated the same way without DNA HSA vectors: pcDNA3.1/GS (G1), pcDNA3.1/V5-His (H1), pcDNA3.1/V5-His with...

Ngày tải lên: 12/08/2014, 18:22

... environment for modeling biological systems and formalizing experimental knowledge Bioinformatics 2006, 22:1805-1807 Bosl WJ: Systems biology by the rules: hybrid intelligent systems for pathway ... [42] Tools for modeling regulatory systems using various formalisms, for example, Boolean, discrete, fuzzy logic and so on Allow model evaluation through simulations, but are not designed for model ... biotechnology for health', contract number LSHG-CT-2003-503269 The information in this document is provided as is and no guarantee or warranty is given that the information is fit for any particular...

Ngày tải lên: 14/08/2014, 08:20

... chloroform were added to the suspension The mixture was maintained under agitation for 60 The sample was centrifuged at 14,000 rpm for and the supernatant was separated for analysis The amount of DNA ... animals vaccinated and challenged with M tuberculosis Formulationsa Miceb Guine-pigsb No vaccination BCG DNAv (naked DNA) DNAhsp65 (naked DNA) Me-DNAhsp65/TDM Me-Prime/boost 74 ± 18 ± 5* 72 ± 38 ± ... intervals); DNA- hsp65 (three intramuscular injection of 100 ug DNA plasmid encoding M leprae hsp65 gene at 2-week intervals); DNAv (plasmid DNA without the hsp65 gene administered at the same scheme for...

Ngày tải lên: 14/08/2014, 19:22

... use of WSNs for real-time information in all aspects of engineering systems are virtually endless, from intelligent building control to health-care systems, environmental control systems, etc ... Technologist, for his priceless help for my research project To me personally, Mr Woo is like a highly respectable elder and his tender loving care and concern (TLC) for me will remain in my heart forever ... ANALYSIS, DESIGN AND IMPLEMENTATION OF ENERGY HARVESTING SYSTEMS FOR WIRELESS SENSOR NODES YEN KHENG TAN M.T.D.(Mechatronics) B.Eng(Hons.) NUS, Singapore A THESIS SUBMITTED FOR THE DEGREE...

Ngày tải lên: 11/09/2015, 09:16

... the use of capillary 1.3 D EVELOPMENT OF DNA S EQUENCING T ECHNOLOGIES 22 electrophoresis (Takahashi et al., 1994; Kheterpal et al., 1996) The current highthroughput automated capillary DNA sequencer ... out parallelly 1.3 D EVELOPMENT OF DNA S EQUENCING T ECHNOLOGIES Adaptor 27 DNA template Template DNA G Primer + A T C + DNA Polymerase G G C Add nucleotides DNA synthesis Intensity Detect incorporated ... quantity of hybridized DNA fragments has shown to not be true (Stekel, 2003) Therefore, it is difficult to use microarray approach for accurate quantification of lowor high-abundance DNA fragments, resulting...

Ngày tải lên: 11/09/2015, 10:01

... coating capillary wall (hydrophillicity or charge) Stability often problematic Table 1.2 Different Modes of High Performance Capillary Electrophoresis CE Mode Capillary zone electrophoresis (CZE) Capillary ... conventional slab gel electrophoresis, and produces longer analysis times Performing electrophoresis in a capillary allows the heat to be effectively dissipated through the capillary walls and ... medicines were studied further by applying Capillary Electrophoresis - Mass Spectrometry (CE-MS) method Nonaqueous Capillary Electrophoresis (NACE) was applied for the separation of alkaloids To improve...

Ngày tải lên: 11/09/2015, 14:27