... regarding the role of the
aVb3 integrin, because it is also reported to suppress
collagen I-induced activation of proMMP-2 [8].
Another MMP inhibitor, reversion-inducing cysteine-
rich protein with ... FEBS
protein in the two cell lines was determined as
described in Materials and methods, and 81 lg of pro-
tein was added to each well. The amount of S100A4 in
the II-11b cells wa...
... hydrophobic
than the interior. In addition, several polar amino acids,
especially arginine, lysine, glutamine and glutamate are
found more frequently at the interface than in the interior.
The degree of hydrophobicity ... nd function of the protein usually involve
dissociating the protein into its subunits and then compar-
ing the properties of the monomeric and oligomeric forms.
Of...
... Use of Mutual Information Based Character Clusters in
Dictionary-less Morphological Analysis of Japanese
Hideki Kashioka, Yasuhiro Kawata, Yumiko Kinjo,
Andrew Finch and
Ezra W. ... The
use of information on character sorts, however,
mitigates this difficulty. This paper presents
our method of incorporating character cluster-
ing based on mutual inf...
... outcome
of the complementation analysis, prove that NirF is a
periplasmic protein in P. pantotrophus.
In uence of variations of conserved residues on
the in vivo activity of NirF
Interestingly, ... and of the
kanamycin insertion mutant nirF strain
(SBN03) in the upper right panel. Growth of
SBN11 (DnirF) is shown in the bottom left
panel and of SBN13 (DnirF) containing...
... Seurin D, Segovia-Quinson B, Hardouin S
& Binoux M (1986) Analysis of serum insulin-like growth
factor binding proteins using western blotting: use of the
method for titration of the binding ... local
effect because binding of PMP-BSA, which binds to
other sites in the ectodomain of these heterodimers,
resulted in the predicted amount of ligand binding.
This loss of bi...
... Royal
Society of Chemistry, Cambridge.
Supporting information
The following supporting information is available:
Fig. S1. Construction of a synthetic gene for 2,5-dia-
mino-6-ribitylamino-4(3H)-pyrimidinone ... were of two types, either with the
two-domain architecture of the RibG protein of Bacil-
lus subtilis or the RibD protein of E. coli, respectively
[18–20], or the singl...
... targets).
Effects of DNA cis-platination on sequence-
specific DNA binding of p73 proteins
We tested the in uence of DNA modification with
cisplatin on the binding of two p73 isoforms, p73d and
p73b, to ... protein
In this work, we studied the effects of cisplatin treat-
ment of various p53DBSs on the sequence-specific
binding of a truncated tetrameric p53 construct lacking
the...
... targeting, we divided it into three tri-glycine segments and
examined the importance of each domain in targeting specificity in vitro.
Replacement of the most C-terminal segment with alanine residues ... membrane protein. We divi-
ded this region into three tri-glycine segments and
examined significance of each portion by in vitro
import assay. Interestingly, only the most C-termina...
... small change in the isoelectric point,
likely caused by the modification of a few amino acid
residues upon binding of aminopentynal. This binding
seems to be nonspecific, as the existence of some micro-
heterogeneity ... rates of inactivation
of 0.1–0.3 min
)1
were determined, the apparent K
I
values
(half-maximal inactivation) were of the order of 10
)5
M
.
DAPY was found to be...
... were interested in using reverse-joined hairpin
ribozymes as building blocks for the construction of
twin ribozymes. In order to evaluate the structural
properties of reverse-joined hairpin ribozymes ... enhanced
coaxial stacking of the two domains in the relevant
variant of the hinged conventional hairpin ribozyme
leading to enrichment of an extended inactive confor-
mation [...