Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc

... FEBS Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants Gabor L. Igloi and Elfriede Schiefermayr Institute of Biology, University of ... between arginine and canavanine for aminoacylation of the plant tRNA transcript was observed when using [ 14 C]-canava- nine. At 0.4 mm canavanine, less than...

Ngày tải lên: 18/02/2014, 13:20

... acknowledged for their assistance with data collection. We thank Annette Kure Andreassen, Marianne Mortensen and Augusta Palsdottir for outstanding technical assistance. References 1 Huang W, Jia J, Edwards ... FEBS Decarboxylation of malonyl-ACP by wild-type and mutant KAS I proteins The ability of the wild-type and mutant KAS I proteins to form acetyl-ACP from malonyl-ACP (Scheme 1) w...

Ngày tải lên: 19/02/2014, 08:20

... completion of the genome sequence of the EPA and DHA producing diatom T. pseudonana [17]. Levels of EPA and DHA in this organism are in the range of 17 and 5%, respectively, when in the exponential phase of ... has recently been isolated from a number of marine organ- isms [12–14]. The final elongation step [of C20 polyun- saturated fatty acids (PUFAs) to C22] catalysed by a D 5...

Ngày tải lên: 20/02/2014, 01:20

... secretion of hBD3 and other antimicrobial peptides by human keratinocytes, after the disappearance of neutrophils and before the re-establishment of the physical barrier, in sterile wounds as well as ... [25], and gastric epithelial cells [26]. hBD1 and hBD2 have predominant antibacterial activity against Gram-negative bacteria and some fungi, whereas hBD3 has a broader spectrum a...

Ngày tải lên: 18/02/2014, 06:20

... cell invasion and metastasis, although their role is dual [41–43]. Both MMPs and TIMPs, as well as the in vivo substrates of a given MMP, can prevent or facilitate the invasion and metastasis process, ... syn- thesis and activation of MMP-2, as well as synthesis of MT1-MMP and TIMP-1, by using an in vitro model with 3D fibrillar and 2D monomeric collagen. In order to reveal w...

Ngày tải lên: 18/02/2014, 11:20

... membrane protein con- sisting of six transmembrane helices and an amino terminal nucleotide binding domain (NBD) [9–11]. It is described as being a ‘half-transporter’ as the canonical ABC transporter ... (3–300 lM) as described in Materials and methods. Labelled protein was visualized and quantified by autoradiography of SDS-PAGE analysis. The amount of bound protein was plot...

Ngày tải lên: 18/02/2014, 18:20

... measurements. 3-(trimethylsilyl)propanesulfonate served as an external standard for 1 H- and 13 C-NMR measurements. Assay of 2,5-diamino-6-ribitylamino-4(3H)- pyrimidinone 5¢-phosphate synthase ... addition of guanylate kinase and pyruvate kinase served to recycle GMP, a by- product of GTP cyclohydrolase II, into GTP). 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢- phosphate syn...

Ngày tải lên: 18/02/2014, 18:20

... reaction was terminated by addi- tion of 0.8 mL of 0.25 m glycine buffer (pH 10.4), and the amount of 4-methylumbelliferone released was determined fluorometrically with an excitation wavelength of 365 ... Riboni M, Manzoni M, Venerando B, Croci G, Preti A, Ballabio A, Tettamanti G et al. (2000) Identification and expression of NEU3, a novel human sialidase associated to the plasm...

Ngày tải lên: 19/02/2014, 06:20

... Exchanging this portion with a repeat of leucine or glutamic acid also caused missorting of Toc75 to the stroma. By contrast, its replacement with repeats of asparagine, aspartic acid, serine, and ... stroma and to the membrane (Fig. 4A, lanes 21–24; Fig. 4B,C) in a way similar to GGA mutant (Fig. 4A, lanes 9–12; Fig. 4B,C). Import of GGE mutant was less efficient than that of o...

Ngày tải lên: 19/02/2014, 07:20

... DABY and PSAO (r ¼ 17) [7]. From this point of view, plant CAOs are more resistant to the inactivation by DAPY than by DABY. Binding of DAPY at the active site of GPAO is dependent on both pH and ionic ... values) in the inactivated enzyme was confirmed. As in the case of DABY, the cofactor topaquinone is not modified by the reaction, as demonstrated by an unchanged quinon...

Ngày tải lên: 19/02/2014, 16:20