- Bc 1: Cân 100 m gb t go vào bình tam giác hay bình nh mc 100 ml, thêm vào 1ml ethanol 95% l c u sau ó cho thêm vào 10 ml NaOH 1N và qua
23 Tà uH ng (608) 48 Thanh Trà (604) 73 Np Áo Vàng (1300) 24 Mùa S 62 (4)49 M t B i (1885)74 Ng c N (170)
25 Lúa Th m (533) 50 504 Mùa (2119) 75 LTH
M 26 27 28 2930 31 32 33 34 35 3637 38 39 4041 42 43 44 45 46 47 48 49 50
Hình 3.5.3: S n ph m khu ch i PCR v i RM21 trên gel agarose 3% a qu n th F2 12844/12845
Hình 3.5.3: S n ph m khu ch i PCR v i RM21 trên gel agarose 3% a qu n th F2 12844/12845 (ti p theo)
Hình 3.5.4: S n ph m khu ch i PCR v i STS-RG64 trên gel agarose 1,5% a qu n th F2 12844/12845 (enzyme c t gi i h nHae III)
Hình 3.5.4: S n ph m khu ch i PCR v i STS-RG64 trên gel agarose 1,5% a qu n th F2 12844/12845 (enzyme c t gi i h nHae III) (ti p theo)
Hình 3.5.5: S n ph m khu ch i PCR v i RM206 trên gel agarose 3% a qu n th F2OM12844/OM12845
Hình 3.5.5: S n ph m khu ch i PCR v i RM206 trên gel agarose 3% a qu n th F2OM12844/OM12845 (ti p theo)
Thoi gian sinh truong
The SAS System 10:25 Thursday, March 11, 2011 1
The GLM Procedure
Class Level Information Class Levels Values
rep 3 1 2 3
treat 39 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39
Number of Observations Read 117 Number of Observations Used 117
The SAS System 10:25 Thursday, March 11, 2011 2 The GLM Procedure
Dependent Variable: TGST
Sum of
Source DF Squares Mean Square F Value Pr > F Model 40 19668.34188 491.70855 533.99 <.0001 Error 76 69.98291 0.92083
Corrected Total 116 19738.32479
R-Square Coeff Var Root MSE TGST Mean 0.996454 0.951224 0.959598 100.8803
Source DF Type I SS Mean Square F Value Pr > F treat 38 19666.99145 517.55241 562.05 <.0001 rep 2 1.35043 0.67521 0.73 0.4837
Source DF Type III SS Mean Square F Value Pr > F
treat 38 19666.99145 517.55241 562.05 <.0001 rep 2 1.35043 0.67521 0.73 0.4837
The SAS System 10:25 Thursday, March 11, 2011 3
The GLM Procedure t Tests (LSD) for TGST
Error Degrees of Freedom 76 Error Mean Square 0.920828 Critical Value of t 1.99167 Least Significant Difference 1.5605
Means with the same letter are not significantly different.
t Grouping Mean N treat A 140.3333 3 16 B 124.0000 3 38 C 120.0000 3 14 D 115.0000 3 27 D 114.3333 3 39 D 114.0000 3 8 D 113.6667 3 28 E 111.6667 3 30 E 111.6667 3 36 F E 110.3333 3 33 F 110.0000 3 10 G 105.3333 3 9 G 105.0000 3 11 G 105.0000 3 34 G 105.0000 3 12 G 105.0000 3 35 G 104.6667 3 32 G 104.0000 3 23
The SAS System 10:25 Thursday, March 11, 2011 4 The GLM Procedure
t Tests (LSD) for TGST
Means with the same letter are not significantly different. t Grouping Mean N treat
H 102.0000 3 15 I 100.3333 3 18 I 100.3333 3 18 I 100.3333 3 2 I 100.0000 3 13
K 90.3333 3 18 L K 90.0000 3 25 L K 90.0000 3 25 L K 90.0000 3 31 L K 90.0000 3 1 L K 90.0000 3 20 L M 88.6667 3 29 N M 87.3333 3 6 N O 86.0000 3 22 N O 86.0000 3 4 P O 85.3333 3 19 P O 85.0000 3 7 P O 85.0000 3 5 P 84.0000 3 3 Q 80.0000 3 26
The SAS System 22:44 Thursday, March 14, 2011 1 The GLM Procedure
Class Level Information Class Levels Values
rep 3 1 2 3
treat 39 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39
Number of Observations Read 117 Number of Observations Used 117
The SAS System 22:44 Thursday, March 14, 2011 2 The GLM Procedure
Dependent Variable: cao cay
Sum of
Source DF Squares Mean Square F Value Pr > F Model 40 2624.307692 65.607692 13.85 <.0001 Error 76 360.000000 4.736842
Corrected Total 116 2984.307692
R-Square Coeff Var Root MSE caocay Mean 0.879369 2.010439 2.176429 108.2564
Source DF Type III SS Mean Square F Value Pr > F treat 38 2611.641026 68.727395 14.51 <.0001 rep 2 12.666667 6.333333 1.34 0.2687
The SAS System 22:44 Thursday, March 14, 2011 3 The GLM Procedure
t Tests (LSD) for caocay
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05
Error Degrees of Freedom 76 Error Mean Square 4.736842 Critical Value of t 1.99167 Least Significant Difference 3.5393
Means with the same letter are not significantly different. t Grouping Mean N treat
A 116.000 3 23 A 116.000 3 26 A 116.000 3 26 B A 115.667 3 3 B A C 114.000 3 33 B A C 113.667 3 7 BD A C 113.000 3 12 BD A C 113.000 3 2 BD A C 113.000 3 13 E BD A C 112.667 3 8 E BD F C 112.333 3 5 E G D F C 111.333 3 30 E G D F C 111.000 3 36 E G D F C 110.667 3 39 E G D F H 110.000 3 21 E G D F H I 109.667 3 38 E G F H I 109.333 3 6 E G F H I 109.333 3 29 G F H I 109.000 3 16
Means with the same letter are not significantly different.
t Grouping Mean N treat G F H I 109.000 3 27 G H I 108.667 3 4 G H I 108.667 3 9 G H I 108.667 3 22 G H I 108.667 3 35 G H I 108.333 3 28 G H I 108.333 3 25 J H I 106.667 3 20 J I 106.333 3 34 J I 106.333 3 32 K J 104.667 3 11 K J 104.333 3 18 K J 104.333 3 24 K J 103.667 3 15 K 102.667 3 17 K 102.667 3 19 K 102.333 3 37 K 102.000 3 31 K 101.333 3 1 L 97.333 3 10 L 97.333 3 14 Bong/bui
The SAS System 22:59 Thursday, March 11, 2011 1 The GLM Procedure
Class Level Information Class Levels Values
rep 3 1 2 3
treat 39 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39
Number of Observations Read 117 Number of Observations Used 117
Source DF Squares Mean Square F Value Pr > F Model 40 859.4700855 21.4867521 17.05 <.0001 Error 76 95.7777778 1.2602339
Corrected Total 116 955.2478632
R-Square Coeff Var Root MSE bong/bui Mean 0.899735 9.758125 1.122601 11.50427
Source DF Type I SS Mean Square F Value Pr > F treat 38 855.9145299 22.5240666 17.87 <.0001 rep 2 3.5555556 1.7777778 1.41 0.2503 Source DF Type III SS Mean Square F Value Pr > F treat 38 855.9145299 22.5240666 17.87 <.0001 rep 2 3.5555556 1.7777778 1.41 0.2503
The SAS System 22:59 Thursday, March 11, 2011 3 The GLM Procedure
t Tests (LSD) for bong/bui
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05
Error Degrees of Freedom 76 Error Mean Square 1.260234 Critical Value of t 1.99167 Least Significant Difference 1.8256
Means with the same letter are not significantly different. t Grouping Mean N treat
A 18.0000 3 13 B A 17.0000 3 24 B A 17.0000 3 24 B C 16.0000 3 17 D C 15.0000 3 2 D C 15.0000 3 32 D C 14.6667 3 35 D C 14.3333 3 8 D 14.0000 3 11 D 14.0000 3 33 E 12.0000 3 6
E 12.0000 3 38 E 12.0000 3 9 E 12.0000 3 9 E 12.0000 3 12 E 12.0000 3 25 E 12.0000 3 34
The SAS System 22:59 Thursday, March 11, 2011 4 The GLM Procedure
t Tests (LSD) for bong/bui
Means with the same letter are not significantly different.
t Grouping Mean N treat E 12.0000 3 16 F E 11.6667 3 36 F E G 11.3333 3 22 F E G 11.3333 3 10 F E G 11.3333 3 20 F H E G 10.6667 3 23 F H E G 10.6667 3 15 F H E G 10.3333 3 7 F H I G 10.0000 3 5 F H I G 10.0000 3 39 F H I G 10.0000 3 1 F H I G 10.0000 3 30 F H I G 10.0000 3 18 J H I G 9.6667 3 19 J H I G 9.6667 3 37 J H I K 9.0000 3 27 J I K 8.3333 3 28 J K 8.0000 3 14 L K 7.3333 3 3 M L 6.0000 3 4 M 5.3333 3 29 Dai bong
rep 3 1 2 3
treat 39 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39
Number of Observations Read 117 Number of Observations Used 117
The SAS System 22:45 Thursday, March 14, 2011 2 The GLM Procedure
Dependent Variable: Dai bong
Sum of
Source DF Squares Mean Square F Value Pr > F Model 40 226.2051282 5.6551282 6.24 <.0001 Error 76 68.8717949 0.9062078
Corrected Total 116 295.0769231
R-Square Coeff Var Root MSE daibong Mean 0.766597 4.000650 0.951949 23.79487
Source DF Type I SS Mean Square F Value Pr > F treat 38 223.0769231 5.8704453 6.48 <.0001 rep 2 3.1282051 1.5641026 1.73 0.1849
Source DF Type III SS Mean Square F Value Pr > F treat 38 223.0769231 5.8704453 6.48 <.0001 rep 2 3.1282051 1.5641026 1.73 0.1849
The SAS System 22:45 Thursday, March 14, 2011 3 The GLM Procedure
t Tests (LSD) for daibong
NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate.
Alpha 0.05
Error Degrees of Freedom 76 Error Mean Square 0.906208 Critical Value of t 1.99167 Least Significant Difference 1.5481
B A 26.3333 3 13 B A 26.3333 3 24 B A C 25.6667 3 29 B C 25.3333 3 27 B C 25.3333 3 18 B C 25.3333 3 25 B C 25.3333 3 21 B D C 25.0000 3 9 E D C 24.6667 3 36 E D C 24.6667 3 20 E D C 24.6667 3 39 E D C 24.6667 3 11 E F D C 24.3333 3 15 E F D C 24.3333 3 19 E F D C 24.3333 3 7 E F D G 23.6667 3 38 E F D G 23.6667 3 3
The SAS System 22:45 Thursday, March 14, 2011 4 The GLM Procedure
t Tests (LSD) for daibong
Means with the same letter are not significantly different. t Grouping Mean N treat
E F H G 23.3333 3 2 E F H G 23.3333 3 6