- ADN tai tdhgp cd kfch thudc khdng qua
2. Thanh turu tao gidng bien doi gen
* Sinh vat bidn ddi gen la sinh vat ma h6 gen cua nd da dugc con ngudi lam bidn ddi cho phu hgp vdi lgi ich ciia minh.
* Cdch lam bidn ddi gen - Dua them mdt gen la (thudng la gen ciia mdt loai khac) vao he gen ^ s i n h vat chuydn gen. - Lam bidn ddi mdt gen cd san trong h6 gen. - Loai bd hoac lam bdt hoat mdt gen nao dd trong he gen.
2. Thanh turu tao gidng bien doi gen bien doi gen
Hogt dpng cua GV
+ Thuyet minh, giai thfch cho tu lieu.
+ Trao ddi thdng tin.
- GV nhan xet, danh gid va cho diem nhdm cd su chudn bj va thuydt minh tdt.
- GV y6u cdu HS: + Quan sat hinh 20.1 va 20.2.
+ Md ta quy trinh tao ddng vat chuyen gen.
Hogt dpng cda HS
- HS boat ddng nhdm:
+ Cac nhdm sdp xdp tu lieu theo tflng chu de. Vf du: Nhdm ddng vat, nhdm thuc vat, nhdm vi sinh vat.
+ Trao ddi nhan bidt nhdm sinh vat chuydn •gen bdng each nao?
+ Vdi tu lieu dudi dang doan phim HS cdn thuydt minh.
- Dai dien cdc nhdm trinh bay -> ldp theo ddi nhan xet.
- Cac nhdm talp hgp tai lieu dd thanh tu lieu chung cho ca ldp.
- Cd nhan nghien cflu hinh 20.1.
- HS hidu va trinh bay dugc cac budc trong quy trinh tao cflu bidn ddi gen san sinh protein ngudi trong sfla.
- Dai dien HS trinh bay -^ ldp nhan xet bd sung.
Hogt dpng cda GV - GV nhan xet, danh
gia.
- GV dua van de.
+ Theo em khd khan hay gap trong quy trinh tao gid'ng sinh vat bidn ddi gen la gi?
+ Em cd de xuat y tudng tao gid'ng sinh vat chuyen gen trong tuang lai.
- GV nhan xet, ddnh gia.
- GV y6u cdu HS nghien cflu SGK dd khai qudt kidn thflc vd cac thanh tuu tao gid'ng bidn ddi gen.
Hogt dpng cua HS
- HS trao ddi nhdm suy
luan.
+ Gdn gen cua cac sinh vat khdc loai se cho ti 16 thanh cdng thdp.
+ Chi phf cao ddn ddn gid thanh san phdm cao. + Trong tuang lai cdn chuyen gen khang benh nan y d ngudi dd ban chd benh nguy hidm ddn tfnh mang. Tao ddng vi khudn cd djnh dam dd lam giau cac vung dat khd cdn, md rdng dien tfch gieo trdng.
Npi dung
a) Tgo dpng vdt chuyen gen
- Lay trflng ra khdi con
cai cho thu tinh trong dng nghiem.
- Tiem gen cdn chuyen vao hgp tfl -> phdt trien thanh phdi.
- Cdy phdi da chuydn gen vao tfl cung con cai khac dd mang thai va sinh de binh thudng. Vf du: Tao ciiu bidn ddi gen san sinh prdtein ngudi trong sfla.
Chudt bach chuydn gen CO gen hoocmdn sinh
Hogt dpng cda GV Hogt dpng cda HS Npi dung
trudng cua chudt cdng nen khd'i lugng gdp ddi so vdi con binh thudng
b) Tgo giong cdy trong Men ddi gen
- Chuyen gen trfl sau tfl
vi khuan vao cay bdng
-^ tao gidng bdng khdng
sau hai.
- Tao gid'ng lua vang cd kha nang tdng. hgp |3 - cardten trong hat.
- Chuyen gen khang sau tfl dau tuong dai vao dau tuong trdng va ngd. - Chuyen gen khang vi rut gay thdi cu vao khoai tay.
c) Tgo ddng vi sinh vgt Men ddi gen
- Vi khuan E.coli dugc
chuyen gen ma hod hooc mdn insulin d ngudi san xuat lugng ldn thudc insulin chfla benh tieu dudng va ha gia thanh cua thudc.
- Chuydn gen tfl xa khuan vao E.coli de san xuat khang sinh.
- Ddng vi sinh yat phan buy rac thai lam phan bdn va phan buy ddu loang chd'ng d nhidm mdi trudng.
4. Kiem tra danh gia
- HS tdm tdt kidn thflc co ban cua bai hgc - GV yeu cdu HS tra ldi cau hdi trac nghiem Cau 1. Phan tfl ADN tdi td hgp la gi?
a) La phan tfl ADN la dugc chuydn vao td bao thd nhan. b) La phan tfl ADN tim thdy trong the nhan cua vi khudn. c) La doan ADN cua td bao cho kdt hgp vdi ADN cua plasmit. d) La mdt dang ADN cau tao n6n cac plasmit cua vi khudn.
Cau 2: Uu thd ndi bat cua kT thuat chuyen gen so vdi cdc phuang phdp lai truyen thdng la
a) D6 thuc bien, thao tdc nhanh, tdn ft thdi gian.
b) Tdng hgp dugc cac phan tfl ADN lai gifla loai nay vdi loai khac. c) San xudt cdc san pham sinh hgc tren quy md cdng nghiep.
d) Lai gifla cdc loai xa nhau trong he thdng phan loai khdng giao phd'i dugc. Cau 3. Khi chuydn mdt gen tdng hgp prdtein cua ngudd vao vi khudn E.coli, cac nha khoa hgc da lam dugc dieu gi cd lgi cho con ngudi?
a) San xudt insulin vdi gid thdnh ha, dung chfla b6nh tidu dudng cho ngudi.
b) Prdtein hinh thanh se lam giam tac hai cua vi khudn ddi vdi con ngudi.
c) Lgi dung kha nang sinh san nhanh, trao ddi chdt manh cua vi khudn dd tdng hgp mdt lugng ldn prdtein dap flng nhu cdu nhieu mat cua con ngudi.
d) Thudn hod mdt chung E.coli dd nudi cdy vao he ti6u hod cua ngudi.
5. Dan do
- Hgc bai tra ldi cau bdi SGK. - Dgc muc "Em cd bidt?" - On tap kidn thflc vd dot bidn.