1. Kiem tra
- Trinh bay quy trinh tao gid'ng bdng phuang phdp gay ddt bidn.
- Trinh bay quy trinh nhan ban vd tfnh d ddng vat va neu y nghTa thuc tidn cua phuang phap nay.
2. Trpng tam
Cdng nghe gen va cac budc cdn tidn hanh trong cdng nghe gen.
3. Bai mdi
GV n6u vdn de: Trong giai doan hien nay chung ta dang cdn lugng ldn thudc chfla benh, vac«n phdng benh, ngudn luang thuc, thuc phdm, nhung gid'ng vat nudi va cay trdng chi cho nang sudt nhat djnh. Vay lam thd nao de giai quydt dugc vdn dd?
- GV dua vao y kidn cua HS dd ddn ddt vao bai hgc.
Hogt ddng 1
TIM HI^U CONG NGHfi GEN
Muc tieu:
- HS ndm dugc khai niem cdng nghe gen, ADN, tai td hop gen, thd
truydn...
- HS trinh bay dugc cdc budc cdn tidn hanh trong kT thuat chuydn gen dua tren sa dd.
- HS van dung If thuyet vao thuc tidn san xudt.
Hogt ddng cda GV
- GV neu cau hdi:
+ KT thuat gen la gi? + Cdng nghe gen la gi?
Hogt ddng cda HS
- HS nghien cun SGK
trang 83 muc I (1) ket hgp vdi kidn thflc sinh hgc ldp 9 dd tra ldi -> ldp nhan xet bd sung.
Ndi dung
Hogt dpng cda GV - GV nhan xet, danh gia
va giup HS hoan thien kidn thflc.
- GV n6u yeu cdu HS: Nhdc lai kidn thflc vd cdng nghe gen.
- GV ddn ddt: Sinh hgc 12 giup HS hidu rd kT thuat chuyen gen, tfl dd tha'y dugc lgi fch cua kT thuat chuyen gen trong ddi sd'ng con ngudi.
- GV gidi thieu tranh so dd cdy gen bang each dung plasmit de gidi thieu khai qudt cac khau chu ydu: Tao ADN tdi td hgp, dua ADN tai td hgp vao td bao nhan, phan
Hogt dpng cda HS
- HS hidu rd ban cdng
nghe gen la mdt trong nhiing ITnh vuc cua cdng nghe sinh hgc.
- HS van dung kie'n thflc sinh hgc 9 tra ldi dugc: + KT thuat gen gdm 3 khau.
+ Cac flng dung cua cdng nghe gen da tao ra cac chung vi sinh vat mdi, tao gid'ng ddng vat, thuc vat bidn ddi gen.
Npi dung
- Cdng nghe gen la quy
trinh tao ra nhflng td bao hoac sinh vat cd gen bj bidn ddi hoac cd them gen mdi.
- Trung tam cua cdng nghe gen la kT thuat tao ADN tai td hgp (kT thuat chuyen gen).
2. Cac budc trong kT thuat chuyen gen thuat chuyen gen
Hogt dpng cda GV
lap ddng td bao chfla ADN tai td hgp.
- GV cho HS quan sat tranh hinh 20 SGV trang 87.
- GV yeu cdu HS tra ldi cau hdi.
+ The truydn la gi? + Tai sao mud'n chuydn gen tfl loai nay sang loai khac lai cdn cd the truydn?
+ ADN tdi td hgp la gi? + Tao ADN tai td hgp bang each nao?
Hogt dpng cda HS
- HS quan sat, phan tfch
tranh hinh.
- HS nghien cflu thdng tin SGK trang 83 muc 2(a).
- Van dung kidn thflc vd ADN, NST.
- Thao luan nhdm dd thdng nha't y kidn tra ldi. - Y6u cdu n6u dugc: + The truyen la ADN (cd the la plasmit, vi rut).
+ Ngudi ta khdng thd cdy true tidp gen tfl loai nay sang loai khac vi mdi loai cd he gen rieng
Npi dung
Hogt dpng cda GV
- GV nhan xet, ddnh
gia.
- GV cdn thdng bdo dap an dung va tim hidu sd nhdm cd kdt qua dung va chua dung dd giup dd nhdm ydu.
- GV yeu cdu HS khdi qudt kidn thflc.
Hogt dpng cda HS
nen cdn cd boat ddng cua thd truyen.
+ ADN tai td hgp dugc tao ra gdm 3 budc, dd la: Tach chidt, cdt, gdn ndi ADN.
- Dai dien HS trinh bay
tren tranh hinh -^ ldp
nhan xet.
Npi dung
- HS tdm tdt kidn thflc
vd the truydn, ADN, tai td hgp, tao ADN tai td hgp.
* The truydn la phan tfl ADN nhd cd kha nang nhan ddi mdt each ddc lap vdi he gen cua td bdo hoac cd the gdn vao he gen cua td bao.
- Loai the truyen la plasmit.
+ Plasmit la phan tfl ADN nhd dang vdng, trong td bao chat cua vi khuan.
+ Plasmit cd kha nang nhan ddi ddc lap vdi he gen cua td bao.
Hogt dpng cda GV
- GV hdi: Tai sao lai
phai cdt the truyen va ADN ciia td bao cho gen bdng loai enzim gidi ban va ndi chung lai vdi nhau?
Hogt dpng cda HS Npi dung
- HS van dung kidn thflc
vd cdu true ADN dd tra ldi.
+ ADN cd trinh tu nucledtit dac hieu.
+ Enzim gidi ban nhan bidt trinh tu nucledtit. + Enzim ndi cac ddu dfnh mdt each dac hieu dd hoan chinh ADN.
- Loai thd truydn la vi rut thuc chat la ADN ciia vi rut da dugc bien ddi. - Loai the truyen la NST nhan tao.
* ADN tai td hgp:
La phan tfl ADN nho dugc ldp rap tfl cdc doan ADN lay tfl cdc td bao khdc nhau.
* Tao ADN tdi td hgp. - Tach chidt thd truydn va gen cdn chuyen ra khdi td bao.
- Xfl If gen bang enzim gidi ban (Restritaza) de tao ra mdt loai ddu dfnh dd khdp ndi cac ADN vdi nhau.
- Dung enzim ligaza de gdn cdc gen thanh ADN tai td hgp.
Hogt dpng cda GV Hoat dpng cda HS Ndi dung - GV nhan xet va sfl
dung thdng tin bd sung dd giang giai vd cdc enzim gidi ban trong cdng nghe gen.