III. HOAT DONG DAY HOC 1 Kiem tra
TAO GIONG NHO CONG NGHE GEN
I. MUC TIEU 1. Kien thiirc 1. Kien thiirc
- HS giai thfch dugc cac khai niem co ban nhu: Cdng nghe gen, ADN, tai td hgp, the truyen, plasmit.
- HS trinh bay dugc cac budc cdn thidt tidn hanh trong kT thuat chuyen gen.
- HS n6u dugc cdc flng dung cua cdng nghe gen trong viec tao ra cac gid'ng sinh vat bidn ddi gen.
2. KT nang
Ren mdt so kT nang:
- Phan tfch, khai quat, suy luan. - Van dung If thuydt vao thuc tidn. - Xu If thdng tin.
II. CHUAN Bj
- Tranh hinh SGK phdng to.
- Tranh hinh 20 SGV trang 87 phdng to.
Bd sung thong tin:
- KT thuat ADN tai td hgp.
KT thuat ADN tdi td hgp la tap hgp nhidu kT thuat dd tao ra mdt gen hoac he gen, cai bidn cdu true gen, he gen nhdm tao ra cac gen mdi rdi chuyen chflng vao trong td bao, ca thd chu nhdm muc dfch san xuat ra cac san pham (Prdtein, enzim...).
- Tao Vecto tdi td hgp
Mud'n dua mdt gen nao dd vao td bao chu cdn cd vat mang gen(vecta tach ddng) va vecto nay phai dugc gdn vdi gen cdn chuydn (vecto tai td hgp).
Vecto tach ddng: Ggi la phuang tien chuydn gen, vecto nay thudng la phan tfl ADN cd kfch thudc nhd, cd kha nang cai gdn vdi cac gen cdn thidt, cd kha nang tu tdi ban, cd kha nang tdn tai trong td bao chu va mang tfn hieu nhan bidt trong td bao chu da mang vecto tai td hgp. Cac loai vecto thudng dung la:
+ Vecto tdch ddng la plasmit. + Vecto tdch ddng la phago.
- Enzim gidi han: la cac enzim cd kha nang nhan bidt mdt doan trinh tu tren phan tfl ADN va cdt ADN d nhflng diem dac hieu. Enzim gidi ban gdm 2 nhdm chfnh.
+ Enzim gidi ban cdt ddu so le: cdt 2 ngudn ADN tao ra cac ddu dfnh dd ndi cac doan ADN bj cdt lai vdi nhau.
+ Enzim gidi han cdt ddu bdng: Khdng tu ndi lai dugc, cdn sfl dung enzim ndi de ndi cdc doan cdt vdi nhau.
- Thanh tuu flng dung cdng nghe gen
+ Tao cay trdng chuydn gen chd'ng sau hai: Gen chfnh dugc sfl dung trong viec chd'ng cdn trung sau hai la gen tdng hcfp protein trfl sau (ddc td) cua vi khudn Bacillins da cdt bdt phdn phu (gen Cry). Gen Cry nay dugc chuyen vao cac cay trdng khac nhau - tao cay trdng khang sau.
+ Ca chua dugc chuyen gen Cry cd kha nang khdng sau due qua rd ret. + Khoai tay dugc chuyen gen Cry cd kha nang khang dugc cac loai bg cdnh cirng.
+ cay trdng chuydn gen khdng thudc trfl cd: Tach hay tdng hgp enzim EPSP rdi chuydn vao cay trdng. Kdt qua tao cay chuyen gen cd ham lugng,
boat do enzimEPSP cao gdp 4 ldn va chdng chju dugc thudc diet cd. Dien binh la cay ngd, bdng, dau tuang...
+ Cay trdng chuydn gen cd mau sdc mdi, tao gidng hoa cd mau sdc khac nhau.