wpw and tachycardia mediated by an accessory pathway

... transfection of scFv antibody gene and cell clone identification PKZ jointly conceived of the study, and coordination, participated in its design and drafted the manuscript All authors read and ... screening of scFv antibody QZX and MXZ participated the study design and data discussion ZHY participated the animal study YW participated the radiosensitivity analysis and in vitro analysis of DNA-PKcs ... DPK3 (30 kD) and DPK4 (32 kD) segments using anti-DPK3-scFv-2 antibody (E) Immunohybridization analysis of DNA-PKcs in HeLa cell protein lysate using anti-DPK3-scFv-2 and anti-DNA-PKcs antibodies...

Ngày tải lên: 09/08/2014, 09:20

... of the audit committee on an ongoing and timely basis and should include an understanding of the principles of and developments in financial reporting and related company law In appropriate cases, ... plan and understand the reasons for any changes, including changes in perceived audit risks and the work undertaken by the external auditors to address those risks; consider the robustness and ... of financial reporting and the audit process by ensuring that the external auditor is independent and objective and does a thorough job, and by fostering a culture and an expectation of effective...

Ngày tải lên: 18/02/2014, 04:20

... FEBS N Poulsen and N Kroger ¨ 29 30 31 32 33 34 lariophycea): mRNA levels controlled by nitrogen source and by the cell cycle J Phycol 36, 702–713 Hildebrand M (2005) Cloning and functional characterization ... should allow analysis of the role of diatom proteins in short-lived cellular processes such as cytokinesis and valve and girdle band formation which are completed in less than h In future, analysis ... ATGTGTGCAAATTGAAGAAC-3¢ and antisense primer 5¢-TTCGAGCTCCGGGGAAACGGTGCCAACTT-3¢, which introduced a NotI site (underlined) and a SacI site (bold) The resulting 592-bp DNA fragment was digested with NotI and SacI and...

Ngày tải lên: 07/03/2014, 21:20

... (A) and MDA levels (D) were measured as described in Materials and Methods CK-MB activity (B) and cTnT (C) levels were quantified by ELISA *P < 0.01 and !P < 0.05 vs sham group; #P < 0.01 and ... Bcl-2 and Bax expression was detected by Western blot as described in Materials and Methods (A), and quantified by normalization to tubulin (B and C) Bcl-2/Bax ratio (D) was calculated by dividing ... participated in animal experiments LYG made fusion protein and evaluated the apoptosis by Flow Cytometry YL and SYC analyzed the results and help writing the manuscript All the authors have read and approved...

Ngày tải lên: 18/06/2014, 19:20

... contact resistance and antireflection coating by an oxidized Mg layer Jonghwan Lee1, Youn-Jung Lee1, Minkyu Ju1, Kyungyul Ryu1, Bonggi Kim1, and Junsin Yi*1,2 School of Information and Communication ... the original idea and modified the first draft of the manuscript JY designed and coordinated the whole work and finalized the manuscript All authors read and approved the final manuscript Acknowledgments ... oxidized and MgO is formed The reflectance of Mg is decreased dramatically after the oxidation The reflectances of Mg200, Mg300, and Mg400 decrease from 25%, 31.62%, and 47.05% to 8.75%, 7.84% and...

Ngày tải lên: 20/06/2014, 21:20

... supported by a grant from the Korean Health 21 R&D Project, Korean Ministry of Health and Welfare (grant number 01-PJ3PG6-01GN11-0002) and by the Korean Science and Engineering Foundation (grant number ... monolayer and explant cultures, and that the transduced fusion protein has a regulatory function for the IL-1 induced catabolic pathway in chondrocytes Materials and methods Reagents Recombinant human ... induced by IL-1, both in monolayer and explant cultures However, Tat-SOD transduction failed to inhibit Metalloproteinase (MMP)-1, -3 and -13 expression or prostaglandin (PG) E2 activation induced by...

Ngày tải lên: 09/08/2014, 08:22

... design, product manufacturing and product use [28], [29] Moreover there exist an established relationship among modularity as an organizational form and heterogeneity of users demands [30] Arguably ... platforms, Android and iPhone respectively, based on proprietary governance models The following subsection will provide insights on this paradox 2.2 Models of governance and modularity in platform -mediated ... framework and cases Research project funded by VISION Era-Net, Helsinki: Finland 17, 2008 [6] Chesbrough, H.W Graceful Exits and Missed Opportunities: Xerox’s Management of its Technology Spinoff Organizations...

Ngày tải lên: 28/06/2015, 18:25

... apoptosis by using 3T3-L1 adipocytes and rat primary adipocytes Adipocyte apoptosis could be induced by troglitazone, pioglitazone or 15-deoxy-D12,14-prostaglandin J2 (PGJ2), and reversed by GW9662, ... Pioglitazone, PGJ2 and Ac-DEVD-CHO were from Cayman Chemical Company (Ann Arbor, MI, USA) Antibodies against Akt-1, phosphorylated Akt-1 (pAkt-1) (Ser473), Bcl2, Bax and Hsp90 were from Santa Cruz Biotechnology, ... xanthohumol and isoxanthohumol on 3T3-L1 cell apoptosis and adipogenesis Apoptosis 12, 1953–1963 15 Yang JY, Della-Fera MA & Baile CA (2008) Guggulsterone inhibits adipocyte differentiation and...

Ngày tải lên: 16/02/2014, 09:20

... for h and were harvested (lanes 1, 2, and 6) and h (lanes 3, 4, and 8) after infection for RNA analysis by Northern blot using a 35Slabelled HA riboprobe Lanes 1, 4, and correspond to deadenylated ... incubated for h before transfection After transfection, the cells were incubated for another 24 h and then infected by Sendaı¨ virus for h Cells were washed and preincubated in a Met and Cys-depleted ... extracted and analysed by Northern blot with a 32P-labelled antisense IFN-b riboprobe The results presented in A, B and C are representative of more than five independent experiments Data in D and E...

Ngày tải lên: 17/03/2014, 10:20

... used to analyze alterations of PAK1 and IL-RI interaction The immunoprecipitation antibody was anti-IL-1RI and the immunoblotting antibody was anti-PAK1 (A) Panel B depicts quantitative analysis ... Piscataway, NJ) Bands were detected by ECF substrate (Amersham Biosciences, Piscataway, NJ) and were quantified using ImageQquant software Statistics Data are represented as mean ± SD and analyzed with ... exposed to IL-1b and IL-1ra as described in Methods, and immunoprecipitation was used to analyze the association between PAK1 and IL-RI The immunoprecipitation antibody was anti-IL-1RI and the immunoblotting...

Ngày tải lên: 19/06/2014, 22:20

... lesions By using this approach, safe and successful ablation of the accessory pathway was achieved Other congenital anatomic defects that manifest in infancy like SVT are Ebstein anomaly and levotransposition ... The disadvantages of this method are the larger size of the catheter and its stiffness We elected to use RF energy and minimized the number of catheters used and the number, power, and duration ... of damage to the coronary arteries and intra-cardiac structures, and technical reasons (patient and catheter size and curves) [7] A large multi-center study by the Pediatric Electrophysiology...

Ngày tải lên: 10/08/2014, 23:20

... characterized by using a specific antibody for the p50 or p65 subunit, and the results indicate that the NF-jB band intensity reduced in the presence of anti-p50 or anti-p65 IgG (Fig 2B, lanes 12 and 13) ... levels of interleukin (IL)-1b and IL-6 in the SP began to rise at h, and reached their peak levels between 48 and 96 h Over the dose range of 0.2–5.0 ngÆmL)1 of SEC1, IL-1b and IL-6 in the SP displayed ... SP and attenuated the febrile response in rabbits (Table 2) Apocynin also inhibited the SEC1-induced nuclear NF-jB expression (Fig 2C, lanes and 4) and its DNA-binding activity (Fig 2D, lanes and...

Ngày tải lên: 19/02/2014, 00:20

... growth medium, and DNA was isolated at 24, 48 and 72 h following serum stimulation and analyzed by agarose gel electrophoresis Preparation of cytoplasmic and nuclear extracts Cytoplasmic and nuclear ... insults, and that both AP-1 [10–12] and CLU [20] are induced and activated in these cells following oxidative stress, a spontaneously immortalized human keratinocyte line, HaCaT (bearing mutant, transcriptionally ... monolayers of HaCaT Neo and HaCaT c-fos cells were cultured for 24, 48 and 72 h in the presence of serum, and DNA isolated from floating and attached cells was analyzed by agarose gel electrophoresis...

Ngày tải lên: 16/03/2014, 02:20

... electrophoresis, and analyzed using an LAS-1000plus luminescent image analyzer (Fujifilm, Tokyo, Japan) We thank Y Takami and H Suzuki for technical support and H K Barman for editorial reading of the manuscript ... is transduced from BCR and membrane-proximal factors (Syk, BTK, BLNK and PLCc2, etc), via mainly PKCd, PKCe and PKCf, to NF-jBs (probably c-Rel and NFp50) This activated signal mediated by BCR ... for DNA content analysis by propidium iodide staining Nuclei were analyzed by flow cytometry (FACSCalibur, Becton Dickinson and Company, Franklin Lakes, NJ, USA), and data for DT40 and E2A)/) (clone...

Ngày tải lên: 16/03/2014, 04:20

... with ad-LacZ as control (lanes and 2), ad-Smad2 (lanes and 4) or ad-Smad3 (lanes and 6) Cells were serum-starved for 24 h and stimulated with ngÆmL)1 TGFb1 (lanes 2, and 6) for 15 Immunoblots ... min, and GST–RBD was used to isolate GTP-loaded RhoA and RhoB from cell lysates Both proteins were monitored by immunoblotting using anti-RhoA and antiRhoB specific serum, and the protein band ... enhanced by overexpression of Smad3 and Smad4 proteins (3.4-fold) and to a lesser extent by overexpression of Smad2 and Smad4 proteins (2.3-fold), and this activity was stimulated further by...

Ngày tải lên: 16/03/2014, 06:20

... one of the mutants by PCR and Southern blot analysis A 1462-bp PCR band corresponding to the extended resistance cassette was found only in the mutant strain, and a 1372-bp PCR band corresponding ... cosmid with oriT and the S coelicolor DdesA mutant, single crossover exconjugants were screened for kanamycin resistance Kanamycin-resistant colonies were isolated and analysed by HPLC for their ... glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling Appl Environ Microbiol 71, 3255–3268 ´ 38 Bikandi J, San Millan R,...

Ngày tải lên: 16/03/2014, 11:20

... substrates, L-DNA(40) and R-DNA(30), were consumed almost completely and ligated by 0.2 lM of LigTk The concentrations of L-DNA(40) and R-DNA(30) were lM each and considerably higher than that of LigTk, ... 72, 9142±9149 23 Sriskanda, V & Shuman, S (1998) Speci®city and ®delity of strand joining by Chlorella virus DNA ligase Nucleic Acids Res 26, 3536±3541 24 Bhagwat, A.S., Sanderson, R.J & Lindahl, ... T-DNA, and 200 nM LigTk The enzyme and other constituents of the reaction mixture were incubated separately at the desired temperature, and reactions were initiated by mixing the two solutions Standard...

Ngày tải lên: 17/03/2014, 11:20

... than HIM) or similarly) References [Haji~v~i and Sgall, 1985] Eva Haji~Wi and Petr SgaU Towards an automatic identification of topic and focus Proceedings of the 2nd Conference of the European ... Manner Directional(from) - Means - Directional(to) Locative An automatic identification of topic, focus and the degrees of communicative dynamism, discussed in a preliminary way by Haji6ova and ... 263-267, 1985 [Sgall, 1986] Petr Sgall, Eva Haji~ov~i and Jarmila Panevov~i The meaning of the sentence in its semantic and pragmatic aspects Ed by J Mey Dordrecht:Reidel - Prague:Academia, 1986...

Ngày tải lên: 18/03/2014, 02:20

... – XPS Spectra of WO3 nanostructures: a) O1 s and b) W4 f axis with an inter-planar distance of 0.38 nm Raman, EDS and XPS analysis confirmed that the chemical structure and oxidation states belong ... the detector; and a computer-controlled XY stage with a spatial resolution of 0.1 µm with two interchangeable gratings (600 and 1800 g mm− 1) and a confocal microscope with 10, 50, and 100× objectives ... nanostructures is a modification of the method reported by Albiter et al for MoO3 nanorods [20] and Therese et al for WO3 nanostructures [18] The main difference between Therese et al method and...

Ngày tải lên: 20/03/2014, 13:08