why evolution is true by jerry coyne
KRONE - What is Patch by Exception
Ngày tải lên :
26/10/2013, 19:15
... even easier.
HOW TO GET THE MOST OUT OF PATCH BY EXCEPTION
Many organisations have successfully implemented the Patch by Exception solution by establishing a
maintenance contract with an installer ...
manage, but that will accommodate the developing technologies of tomorrow.
WHAT IS PATCH BY EXCEPTION?
Patch by Exception is a product feature of KRONE's HIGHBAND 8 and 10 modules that allows ... changes without the need for assistance from an
installer.
To date this has been achieved by assigning a hub port and an extension port to each individual within an
organisation. If an individual...
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Tài liệu Top 10 Reasons Why TCP Is Reliable ppt
Ngày tải lên :
21/12/2013, 04:18
... during transmission. The segment is discarded by the
receiving process, as if it were never received. Bad data is never processed by TCP when the checksums do not
match.
Summary
The Transmission Control ... Knowledge T
raining LLC. All rights reserved.
Page 11
6. Retransmission of Missing Information
TCP retransmits missing information by using missing acknowledgements. If a TCP process sends data and
receives ... the client is telling the service that 5840 bytes of buffer space is available for the serv-
ice if and when it decides to send data to the client. The service is obliged to send 5840 bytes or...
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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf
Ngày tải lên :
15/02/2014, 01:20
... the tissues
producing it. In response to tissue injury, such as
hepatic and renal injury, the inactive single-chain HGF
is converted to a two-chain form exclusively in
the injured tissue. This ... exclusively in
the injured tissue. This conversion is mediated by ser-
ine protease activity, which is induced in the injured
tissue [28]. The two-chain form is required for the bio-
logical activity ... TMPRSS13.
Proteolytic activation of pro-HGF by TMPRSS13
Pro-HGF is proteolytically activated by matriptase
and hepsin, and the protease activity is inhibited by
HAI-1 [9,10,18]. Therefore, we examined...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
Ngày tải lên :
18/02/2014, 04:20
... followed by Strep-tag II
was isolated by BamHI digestion and inserted into the
BamHI site of pmGFP–calpain 7.
To construct pCMV3xFLAG–IST1 and pGEX–IST1, an
IST1 cDNA fragment was amplified by PCR, ... autolysis. Therefore, it is premature
to draw conclusions regarding the mechanism of the
enhancing effects of IST1 on mGFP–calpain 7–Strep
autolysis in vitro.
Both mGFP–calpain 7 and IST1 have ... FLAG–IST1 did not
enhance the autolysis of mGFP–calpain 7 in cultured
cells. On the other hand, overexpression of FLAG–
IST1
DMIM1,2
suppressed the autolysis. This observation
might be explained by...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
Ngày tải lên :
18/02/2014, 12:20
... K
m
values is exclu-
sively displayed by the tetramer forms, and low-
efficiency kinetics with high K
m
values is displayed by
the dimer forms. These observations strengthen the
previous hypothesis that ... K
m
is the Michaelis
constant and K
0.5
is the substrate concentration where
v = 0.5 V
max
. When n = 1, there is no co-operativity, and
K
0.5
= K
m
.
Acknowledgements
This work was supported by ... behaviour after dilution to 6 lgÆmL
)1
indicate that
dissociation of the tetramer to the dimer is a slowly
progressing process. This is supported by the kinetic
behaviour of the recombinant enzyme...
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Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx
Ngày tải lên :
18/02/2014, 13:20
... was
detected using MUPP1 antibodies.
Immunohistochemistry
Mice were raised and maintained according to governmen-
tal and institutional care instructions. Immunohistochemis-
try was carried out on 14 lm ... different odorant molecules is governed by the diverse array of
olfactory receptors expressed by olfactory sensory neurons in the nasal
epithelium. Olfactory receptors consist of seven transmembrane ... between
MUPP1 and OR2AG1 is inhibited by over-expressing
the interacting PDZ domains, the resulting response of
OR2AG1 to odorant is modified in that the rapid
decay of signal is impaired.
We finally...
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Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx
Ngày tải lên :
18/02/2014, 18:20
... cancer. The activity of cyclin dependent
kinase 4 is controlled by multiple mechanisms, including phosphorylation
of tyrosine 17. This site is equivalent to tyrosine 15 of cyclin dependent
kinase ... in HeLa lysates. C-YES
is a 62 kDa nonreceptor tyrosine kinase of the Src
family that is expressed in a wide range of tissues [24].
The N-terminus of C-YES is dually myristoylated and
palmitoylated ... thyroid epithelial cells: activation by TSH, inhi-
bition by TGFbeta, and phosphorylations of its
subunits demonstrated by two-dimensional gel electro-
phoresis. Exp Cell Res 291, 135–149.
35 Sexl...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
Ngày tải lên :
19/02/2014, 00:20
... Germany).
SN-50 and PDTC were dissolved in distilled water. Wort,
HIMO, MK 886 and ETYA were dissolved in dimethylsulf-
oxide. Apocynin was dissolved in ethanol. Polymycin B was
dissolved in normal saline. ... cytokine synthesis
and febrile response induced by SEC1 is dependent on
NADPH oxidase and not on PI3K ⁄ Akt or 5-LOX ⁄
FLAP because these phenomena are attenuated by
apocynin and not by ETYA, MK ... and
IL-6 that is blocked by the NF-jB inhibitor (PDTC or
SN-50). Moreover, the NF-jB binding ability is abro-
gated by binding site competition and antibody super-
shift analysis. These findings...
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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf
Ngày tải lên :
19/02/2014, 06:20
... precipitated DNA was analyzed by 1% agarose gel electro-
phoresis. DNA was visualized by staining with ethidium bromide. Results are represented by negative images. Asterisks indicate nucleic
acids ... and were isolated as a single peak fraction by
a 0.1–1.0 m NaCl linear gradient in R buffer. Histone H1,
which exists in the DNA affinity fraction, was separated as
a major component stepwise elution ... nuclear foci
linked to phosphorylated histone H2AX (c-H2AX) [3],
which is responsible for the redistribution of repair
factors to DSB sites [4], although it is dispensable
for initial damage recognition...
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Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf
Ngày tải lên :
20/02/2014, 02:21
... multigene
family is modulated by distal cis elements. J Biol Chem
271, 28146–28153.
24 Sharma S & Gopinathan KP (1996) Role of TATATAA
element in the regulation of tRNA
Gly
1
gene expression in
Bombyx ... examined by including heparin (20 lgÆmL
)1
) in the binding reaction (lanes 5,
6, both panels). The complex formation was analysed by electrophoresis on 4% polyacylamide (nondenaturing) gels and visualized ... as marked. The heparin-resistant complex on tRNA
Gly
1
-1 (left) is marked by an arrow; ++ denotes 6 lg of protein. (B) The
specificity of complex formation was examined by the competition with...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
Ngày tải lên :
20/02/2014, 11:20
... this gene is controlled by Ets-1 and requires the
presence both of the Sp1 and Sp3 transcription factors. The
presence of this complex multicomponent PRD in the fgl2
proximal promoter is consistent ... pre-
sented in this study suggest that this PRD is necessary for
the inducible expression of fgl2, we plan to undertake
additional studies aimed at more precisely elucidating
specific mechanisms regulating ... not formed by the
binding of Sp1 or Sp3. However, we cannot rule out a Sp1
related transcription factor or an unknown factor at this
time. Future studies are required to address this issue.
We...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
Ngày tải lên :
21/02/2014, 01:21
... SODs is not governed by
one or even two residues, but is most likely accomplished by
the concerted effects of a combination of key residues.
Further analysis of these and other mutant SODs is
currently ... electrophoresis (PAGE)
Native 8% polyacrylamide gels (acrylamide : N,N¢-methyl-
enebisacrylamide, 29 : 1, w/w) containing NaCl/Tris,
pH 8.8 utilized a Tris/glycine electrophoresis buffer system
consisting ... N-terminal amino
acid residues. SOD is released from GST-SOD fusion
proteins by cleavage with FXa (Fig. 2C). Although this
method is less efficient than cleavage by thrombin [24] the
released SOD...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
Ngày tải lên :
22/02/2014, 04:20
... semiquantitatively by t his method. The effects of
enzymes, inhibitors and other reagents on the cell cycle–
dependent interaction could also be examined very easily by
means of t his method. This method is ... meiotic division until they meet sperm. In that
phase, the nuclear envelope is dispersed in the cytop lasm as
nuclear envelope precursor vesicles. Cell cycle progression is
triggered by fertilization, ... shown by sperm chromatin
in vitro ([6,13]; this study), and by mitotic phase chromo-
somes f rom C HO cells [5]. Regulation of the binding of
LBR to chromatin by phosphorylation was shown in this
study...
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Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx
Ngày tải lên :
22/02/2014, 07:20
... preimplantation
embryos in vitro [8,9]. However, no detail is known about
the mechanism of the disruption and its relationship to the
induction of apoptosis.
Our hypothesis is that oxidative stress contributes to ... occurrence. In this paper, we
describe trophoblast cell apoptosis during in vitro
incubation of human fetal membrane tissue, transcription
of apoptosis-related genes, inhibition of apoptosis by
Correspondence ... anti-inflammatory
function, and that apoptosis suppression by glucocorticoid
in the tissue may also be affected by suppression of specific
gene functions, such as c-Jun.
It is known that there is an increase in fetal...
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Tài liệu Báo cáo Y học: Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine docx
Ngày tải lên :
22/02/2014, 07:20
... VSMC
death.
ACKNOWLEDGEMENTS
This work is supported by grants from the National Health and
Medical Research Council (NH & MRC) of Australia. KB is supported
by the Deutsche Forschungsgemeinschaft and the Bundesministerium
fu
¨
r ... the number of apoptotic cells. This increase in
apoptosis was accompanied by activation of caspase-3.
Homocysteine, a known risk factor for cerebrovascular
disease, increased Ab-induced toxicity ... caspase-3.
Cerebral amyloid angiopathy (CAA) is one of the morpho-
logical hallmarks of Alzheimer’s disease. However, CAA is
also seen in normal ageing. There is increasing evidence that
CAA may underlie...
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