titration of potassium permanganate against oxalic acid

Ngày tải lên: 07/07/2014, 00:20

... assay of Abs against teichoic acid to 72% for assays of antibody to peptidoglycan and α-toxin. The specificity of assays for antibody to cell wall antigens (peptidoglycan and teichoic acid) ... basis of their immunoblot profile against total S. aureus cell lysate as well as their serum IgG response to cell wall antigens peptidoglycan and teichoic acid. Whether the presence of antibodies ... quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254. 17. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage...

Ngày tải lên: 02/11/2012, 11:08

... 2.7: Hiệu quả kích kháng của oxalic acid lên tỉ lệ diện tích vết bệnh trên lá (%) ở các thời điểm 40, 50 và 60 NSKS 42 Hình 2.8: Hiệu quả kích kháng của oxalic acid lên tỉ lệ bệnh thối cổ bông ... 2.13: Hiệu quả kích kháng của oxalic acid lên tỉ lệ diện tích vết bệnh trên lá (%) ở các thời điểm 40, 50 và 60 NSKS 50 Hình 2.14: Hiệu quả kích kháng của oxalic acid lên tỉ lệ bệnh thối cổ bông ... 2001) và mô học (Huỳnh Minh Châu và ctv,2001). 6. Đặc tính oxalic acid và tác động của nó đối với cây trồng Tên hóa học: OXALIC ACID Công thửc hóa học: Thường tồn tại ở dạng ngm 2 phõn t...

Ngày tải lên: 10/04/2013, 21:05

... amount of precipitation in the presence of high concentrations of Arg-HCl in the absence of the chaperone precluded analysis of the effect of this concentration on the protective ability of aB-crystallin. Reduction-induced ... with a number of functional groups, including the aromatic side chains of some amino acids, through a stacking mechanism [42]. The interaction of arginine with aromatic amino acids of aB-crystallin ... chaperone activity of aB-crystallin [27], which was reported to occur via enhancement of the dynamics of subunit assembly [28]. However, to date there have been no reports of the effects of such compounds...

Ngày tải lên: 18/02/2014, 16:20

... potential role of cAMP in mediating the effects of CT on endosome acidification [13]; and (c) the effects of CT were not observed when endosome acidification was measured in the absence of ATP, possibly ... action of cholera toxin under the con- trol of endosomal acidity was confirmed using cellular systems in which modification of the expression levels of cathepsin D, either by transfection of the ... endosomal acidifi- cation at the early stage of CT action. Role of endosomal acidification and cathepsin D in CT action To assess whether the aspartic acid protease cathep- sin D and endosomal acidity...

Ngày tải lên: 19/02/2014, 02:20

... Murakami 2 and Kenji Aoki 2 1 Division of Science of Biological Resources, Graduate School of Science and Technology, 2 Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, ... (A) The reaction mixture consisted of 2.9 mL of 100 m M sodium /potassium phosphate buffer (pH 7.5), 0.1 mL of 5 m M 4-amino-3-hydroxybenzoic acid, and 0.05 mL of the crude extract (3575% ammonia ... 3-hydroxyanth- ralinic acid differs from that in the pathway for 4-amino- 3-hydroxybenzoic acid. The N-terminal amino acid sequence of the purified enzyme d id not show significant levels of identity to sequences of...

Ngày tải lên: 19/02/2014, 16:20

... terms of assembly of fragments is discussed. Results CD spectra Secondary structure of SNase and its mutants was deter- mined by CD with spectrapolorimetry measurements. The approximate fraction of ... the sum of fractional multiples of the reference spectra for each structural type. Figure 1A shows the CD spectra of W140A, E142O and K133A. There are no significant differences from that of the ... the secondary structure of the protein disintegrated. Using the peak at 222 nm of the wild-type protein as an index of helical conformational stability, this CD spectrum of W140O suggests a decrease...

Ngày tải lên: 20/02/2014, 01:20

... The switch regions of EF-G. (A) Switch I region of S. aureus EF-G. The ordered switch I helix packs against helix A G . The 2F o )F c map is contoured at 1r. (B) Comparison of switch I in EF-G ... and GTP forms of EF-Tu. (C) F o )F c omit map of the switch II region of S. aureus EF-G contoured at 3r. Omitted residues are shown in yellow stick representation. (D) Comparison of the switch ... structure of S. aureus EF-G to 1.9 A ˚ resolution, allowing us to examine the generality of conclusions drawn from the T. thermophilus EF-G structures and to pinpoint the role of amino acids that...

Ngày tải lên: 06/03/2014, 22:21

... Netherlands Benzoic acid is important for the food industries. Along with other weak acids such as sulfite and sulfur dioxide, sorbic acid, acetic acid, propionic acid and lactic acid, benzoic acid is used ... 75% of their concentration in the absence of benzoic acid (Table 3). One striking difference between the two steady-states is that the concentrations of the weak acids in the tricarboxylic acid ... absence of benzoate. Equation 7 shows that the export of 1 mol of benzoate leads to an extra O 2 con- sumption of 1 ⁄ (P ⁄ O) = 0.68 mol. If the exporter were to export benzoic acid instead of the...

Ngày tải lên: 07/03/2014, 04:20

... Germany). One unit (U) of tetrathionate hydrolase was defined as the amount of protein required for the hydrolysis of 1 lmol of S 4 O 6 2– in 1min. Other enzyme assays Acid phosphatase activity ... measured spectrophotometrically at 405 nm. One unit of the acid phosphatase activity was defined as the amount of protein which catalysed the formation of 1 lmol of p-nitrophenol in 1 min. Glc6P dehydrogenase ... enzyme. The production of NADPH was determined spectrophotometrically at 340 nm. One unit of Glc6P dehydrogenase was defined as the amount of protein that reduced 1 lmol of NADP + in 1 min. Succinate...

Ngày tải lên: 07/03/2014, 14:20

... K m and V max of various amino acids as a function of the membrane potential in PAT2 expressing oocytes. Membrane potential dependency of the apparent K m (A) and V max (B) values of b-alanine ... Uptake of L -[ 3 H]proline (100 l M ) was measured at pH 6.5 for 10 min in the presence of unlabeled amino acids and derivatives at a fixed concentration of 10 m M andaregivenaspercentageinhibitionofthecontroluptakemeasuredintheabsenceofinhibiton. Data ... specificity of mPAT2. (A,B) Representative current traces of oocytes expressing mP AT2 in response to perfusion with 20 m M of the different substrates in Na + -free buffer at pH 6.5. (C,D) Inhib ition of...

Ngày tải lên: 07/03/2014, 16:20

... Properties of the enzymatic steps/enzymes of the a-oxidation pathway. The table gives an overview of the present knowledge of some of the properties of the enzymes involved in the initial degradation of ... P.P. (1996) a-Oxidation of 3-methyl-substituted fatty acids in rat liver. Production of formic acid instead of CO 2 , cofactor requirements, subcellular localization and formation of a 2-hydroxy-3-methyl- acyl-CoA ... correlation between the level of phytanic acid accumulation and the severity of the clinical symptoms. Interestingly, 30–40% of the patients are born with an absence of one of the metacarpals or metatarsals...

Ngày tải lên: 08/03/2014, 02:20

... similar proportions of phyllosilicates in the V 2 mm soil fraction and of goethite and haematite in the clay fraction. The greater content of illite and the moderate quantity of smectite provide ... we designed an investigation to exam- ine the effect of the simultaneous presence of Pb and Cd on sorption behavior to acidic A horizons of tem perate soils from Spain. Specifically, we have undertaken ... ionic strength of the solution as calculated by Eq. (1). S. Serrano et al. / Geoderma xx (2004) xxx–xxx4 ARTICLE IN PRESS Competitive sorption of cadmium and lead in acid soils of Central Spain S....

Ngày tải lên: 15/03/2014, 23:22

... . Fatty acid methyl esters of total lipids from chloroform extracts of yeast cells were prepared by alkaline transme- thylation using 2 mL of 0.1 M sodium methoxide for 5 min at 90 °C. Preparation of ... fatty acids into their lipids. In absence of exogenous fatty acids, the yeast expressing the C. alpina acetylenase gene converted up to 0.65% of their fatty acids into 1 8:2 in a ratio of D12(E) to ... ontrol of a truncated version (FP1) of the seed-specific napin promoter [10]. Transfor- mation and cultivation of the A. thaliana plants was as described earlier [11]. All fatty acid analyses of the transformants...

Ngày tải lên: 16/03/2014, 18:20

... nonspe- cific and is capable of utilizing a variety of 2-ketoacids. The active site of the enzyme contains eight conserved polar amino acids and we have mutated each of these in order to dissect ... Purification and properties of the acetohydroxy acid isomeroreductase of Salmonella typhimurium. J Biol Chem 244, 1118–1127. R. Tyagi et al. Mutagenesis of the active site of E. coli KARI FEBS Journal ... apparent K d for [Mg 2+ ] of 2.06 ± 0.38 mM. R. Tyagi et al. Mutagenesis of the active site of E. coli KARI FEBS Journal 272 (2005) 593602 ê 2005 FEBS 597 of 8.0). When each of these residues is...

Ngày tải lên: 16/03/2014, 18:20

... polyadenylation site. The cDNA sequence contained an ORF of 414 bp coding for a polypeptide of 137 amino acids. The deduced amino acid sequence of the zebrafish CRAB- PIb showed highest identity to ... distribution of the brain-type fatty acid- binding protein (fabp7a) mRNA is localized to the pariventricular grey zone of the optic tectum of the adult zebrafish brain [24]. Similar pat- terns of mRNA ... levels of crabp1b transcripts may be below the level of sensi- tivity of detection by the technique of tissue section in situ hybridization. Discussion The differentiation and development of primary...

Ngày tải lên: 16/03/2014, 22:20

Ngày tải lên: 17/03/2014, 10:20

Ngày tải lên: 17/03/2014, 17:20

Ngày tải lên: 23/03/2014, 06:20

Ngày tải lên: 23/03/2014, 09:21