titration of potassium permanganate against oxalic acid
Báo cáo y học: "A comparative analysis of antibody repertoire against Staphylococcus aureus antigens in Patients with Deep-Seated versus Superficial staphylococcal Infections"
Ngày tải lên :
02/11/2012, 11:08
... assay of Abs against
teichoic acid to 72% for assays of antibody to
peptidoglycan and α-toxin. The specificity of assays for
antibody to cell wall antigens (peptidoglycan and teichoic
acid) ... basis of their immunoblot profile
against total S. aureus cell lysate as well as their serum IgG
response to cell wall antigens peptidoglycan and teichoic
acid. Whether the presence of antibodies ... quantities of protein utilizing the principle of protein-dye
binding. Anal Biochem 1976, 72:248-254.
17. Laemmli UK. Cleavage of structural proteins during the assembly of
the head of bacteriophage...
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ứng dụng chất kích kháng CuCl2 và Oxalic Acid
Ngày tải lên :
10/04/2013, 21:05
... 2.7: Hiệu quả kích kháng của oxalic acid lên tỉ lệ diện tích vết bệnh trên lá (%) ở các
thời điểm 40, 50 và 60 NSKS 42
Hình 2.8: Hiệu quả kích kháng của oxalic acid lên tỉ lệ bệnh thối cổ bông ... 2.13: Hiệu quả kích kháng của oxalic acid lên tỉ lệ diện tích vết bệnh trên lá (%) ở các
thời điểm 40, 50 và 60 NSKS 50
Hình 2.14: Hiệu quả kích kháng của oxalic acid lên tỉ lệ bệnh thối cổ bông ... 2001) và mô học (Huỳnh Minh Châu và ctv,2001).
6. Đặc tính oxalic acid và tác động của nó đối với cây trồng
Tên hóa học: OXALIC ACID
Công thửc hóa học: Thường tồn tại ở dạng ngm 2 phõn t...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf
Ngày tải lên :
18/02/2014, 16:20
... amount of precipitation in
the presence of high concentrations of Arg-HCl
in the absence of the chaperone precluded analysis of
the effect of this concentration on the protective
ability of aB-crystallin.
Reduction-induced ... with a number of functional
groups, including the aromatic side chains of some
amino acids, through a stacking mechanism [42]. The
interaction of arginine with aromatic amino acids of
aB-crystallin ... chaperone activity of aB-crystallin
[27], which was reported to occur via enhancement of
the dynamics of subunit assembly [28]. However, to
date there have been no reports of the effects of such
compounds...
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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity pdf
Ngày tải lên :
19/02/2014, 02:20
... potential role of cAMP in
mediating the effects of CT on endosome acidification
[13]; and (c) the effects of CT were not observed when
endosome acidification was measured in the absence of
ATP, possibly ... action of cholera toxin under the con-
trol of endosomal acidity was confirmed using cellular systems in which
modification of the expression levels of cathepsin D, either by transfection
of the ... endosomal acidifi-
cation at the early stage of CT action.
Role of endosomal acidification and cathepsin D
in CT action
To assess whether the aspartic acid protease cathep-
sin D and endosomal acidity...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
Ngày tải lên :
19/02/2014, 16:20
... Murakami
2
and Kenji Aoki
2
1
Division of Science of Biological Resources, Graduate School of Science and Technology,
2
Department of Biofunctional Chemistry,
Faculty of Agriculture, Kobe University, ... (A) The reaction mixture
consisted of 2.9 mL of 100 m
M
sodium /potassium phosphate buffer
(pH 7.5), 0.1 mL of 5 m
M
4-amino-3-hydroxybenzoic acid, and
0.05 mL of the crude extract (3575% ammonia ... 3-hydroxyanth-
ralinic acid differs from that in the pathway for 4-amino-
3-hydroxybenzoic acid.
The N-terminal amino acid sequence of the purified
enzyme d id not show significant levels of identity to
sequences of...
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- 1
Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
Ngày tải lên :
20/02/2014, 01:20
... terms of assembly of fragments is discussed.
Results
CD spectra
Secondary structure of SNase and its mutants was deter-
mined by CD with spectrapolorimetry measurements.
The approximate fraction of ... the sum of fractional multiples
of the reference spectra for each structural type.
Figure 1A shows the CD spectra of W140A, E142O
and K133A. There are no significant differences from
that of the ... the
secondary structure of the protein disintegrated. Using
the peak at 222 nm of the wild-type protein as an index
of helical conformational stability, this CD spectrum of
W140O suggests a decrease...
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- 0
Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
Ngày tải lên :
06/03/2014, 22:21
... The switch regions of EF-G. (A) Switch I region of S. aureus EF-G. The ordered switch I helix packs against helix A
G
. The 2F
o
)F
c
map is contoured at 1r. (B) Comparison of switch I in EF-G ... and GTP forms of EF-Tu. (C) F
o
)F
c
omit map of the switch II
region of S. aureus EF-G contoured at 3r. Omitted residues are shown in yellow stick representation. (D) Comparison of the switch ... structure of S. aureus EF-G to 1.9 A
˚
resolution, allowing us to examine the generality of
conclusions drawn from the T. thermophilus EF-G
structures and to pinpoint the role of amino acids
that...
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Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx
Ngày tải lên :
07/03/2014, 04:20
... Netherlands
Benzoic acid is important for the food industries.
Along with other weak acids such as sulfite and sulfur
dioxide, sorbic acid, acetic acid, propionic acid and
lactic acid, benzoic acid is used ... 75% of their concentration in the absence of
benzoic acid (Table 3).
One striking difference between the two steady-states
is that the concentrations of the weak acids in the
tricarboxylic acid ... absence of benzoate. Equation 7 shows that the
export of 1 mol of benzoate leads to an extra O
2
con-
sumption of 1 ⁄ (P ⁄ O) = 0.68 mol. If the exporter were
to export benzoic acid instead of the...
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Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot
Ngày tải lên :
07/03/2014, 14:20
... Germany). One unit
(U) of tetrathionate hydrolase was defined as the amount of
protein required for the hydrolysis of 1 lmol of S
4
O
6
2–
in
1min.
Other enzyme assays
Acid phosphatase activity ... measured
spectrophotometrically at 405 nm. One unit of the acid
phosphatase activity was defined as the amount of protein
which catalysed the formation of 1 lmol of p-nitrophenol in
1 min. Glc6P dehydrogenase ... enzyme. The production of NADPH was determined
spectrophotometrically at 340 nm. One unit of Glc6P
dehydrogenase was defined as the amount of protein that
reduced 1 lmol of NADP
+
in 1 min. Succinate...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
Ngày tải lên :
07/03/2014, 16:20
... K
m
and V
max
of various amino acids as a function of
the membrane potential in PAT2 expressing oocytes. Membrane
potential dependency of the apparent K
m
(A) and V
max
(B) values of
b-alanine ... Uptake of
L
-[
3
H]proline (100 l
M
) was measured at pH 6.5 for 10 min in the presence of unlabeled amino acids
and derivatives at a fixed concentration of 10 m
M
andaregivenaspercentageinhibitionofthecontroluptakemeasuredintheabsenceofinhibiton.
Data ... specificity of mPAT2. (A,B) Representative current traces of oocytes expressing mP AT2 in response to perfusion with 20 m
M
of
the different substrates in Na
+
-free buffer at pH 6.5. (C,D) Inhib ition of...
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Báo cáo khoa học: Alpha-oxidation of 3-methyl-substituted fatty acids and its thiamine dependence pptx
Ngày tải lên :
08/03/2014, 02:20
... Properties of the enzymatic steps/enzymes of the a-oxidation pathway. The table gives an overview of the present knowledge of some of the
properties of the enzymes involved in the initial degradation of ... P.P.
(1996) a-Oxidation of 3-methyl-substituted fatty acids in rat liver.
Production of formic acid instead of CO
2
, cofactor requirements,
subcellular localization and formation of a 2-hydroxy-3-methyl-
acyl-CoA ... correlation
between the level of phytanic acid accumulation and the
severity of the clinical symptoms. Interestingly, 30–40%
of the patients are born with an absence of one of the
metacarpals or metatarsals...
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Competitive sorption of cadmium and lead in acid soils of central spain
Ngày tải lên :
15/03/2014, 23:22
... similar proportions of phyllosilicates in the V 2
mm soil fraction and of goethite and haematite in the
clay fraction. The greater content of illite and the
moderate quantity of smectite provide ... we designed an investigation to exam-
ine the effect of the simultaneous presence of Pb and
Cd on sorption behavior to acidic A horizons of
tem perate soils from Spain. Specifically, we have
undertaken ... ionic strength of the solution as calculated by Eq. (1).
S. Serrano et al. / Geoderma xx (2004) xxx–xxx4
ARTICLE IN PRESS
Competitive sorption of cadmium and lead in acid soils of
Central Spain
S....
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Báo cáo khoa học: Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes pptx
Ngày tải lên :
16/03/2014, 18:20
... .
Fatty acid methyl esters of total lipids from chloroform
extracts of yeast cells were prepared by alkaline transme-
thylation using 2 mL of 0.1
M
sodium methoxide for 5 min
at 90 °C. Preparation of ... fatty acids
into their lipids. In absence of exogenous fatty acids, the
yeast expressing the C. alpina acetylenase gene converted up
to 0.65% of their fatty acids into 1 8:2 in a ratio of D12(E)
to ... ontrol of a truncated version
(FP1) of the seed-specific napin promoter [10]. Transfor-
mation and cultivation of the A. thaliana plants was as
described earlier [11]. All fatty acid analyses of the
transformants...
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Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot
Ngày tải lên :
16/03/2014, 18:20
... nonspe-
cific and is capable of utilizing a variety of 2-ketoacids. The active site
of the enzyme contains eight conserved polar amino acids and we have
mutated each of these in order to dissect ... Purification and
properties of the acetohydroxy acid isomeroreductase of
Salmonella typhimurium. J Biol Chem 244, 1118–1127.
R. Tyagi et al. Mutagenesis of the active site of E. coli KARI
FEBS Journal ... apparent
K
d
for [Mg
2+
] of 2.06 ± 0.38 mM.
R. Tyagi et al. Mutagenesis of the active site of E. coli KARI
FEBS Journal 272 (2005) 593602 ê 2005 FEBS 597
of 8.0). When each of these residues is...
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Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebrafish genome by subfunctionalization of tissue-specific expression doc
Ngày tải lên :
16/03/2014, 22:20
... polyadenylation site.
The cDNA sequence contained an ORF of 414 bp
coding for a polypeptide of 137 amino acids. The
deduced amino acid sequence of the zebrafish CRAB-
PIb showed highest identity to ... distribution of the
brain-type fatty acid- binding protein (fabp7a) mRNA
is localized to the pariventricular grey zone of the optic
tectum of the adult zebrafish brain [24]. Similar pat-
terns of mRNA ... levels
of crabp1b transcripts may be below the level of sensi-
tivity of detection by the technique of tissue section
in situ hybridization.
Discussion
The differentiation and development of primary...
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