the special administrative region of hong kong

... language of instruction in Hong Kong Hong Kong: The Cosmos Cheung, HN 1972 A Grammar of Cantonese Spoken in Hong Kong Hong Kong: The Chinese University Press Curriculum Development Council of Hong Kong ... general use of government funding on the promotion of the “biliterate and trilingual” policy sufficiently reflects the mainstream view on the teaching language of the Hong Kong society Besides the regular ... stage of education The Language Fund of SCOLAR was established to support proficiency in the use of the Chinese (including spoken Putonghua) and English languages by the people of Hong Kong, ...

Ngày tải lên: 20/06/2014, 21:20

... language of instruction in Hong Kong Hong Kong: The Cosmos Cheung, HN 1972 A Grammar of Cantonese Spoken in Hong Kong Hong Kong: The Chinese University Press Curriculum Development Council of Hong Kong ... general use of government funding on the promotion of the “biliterate and trilingual” policy sufficiently reflects the mainstream view on the teaching language of the Hong Kong society Besides the regular ... stage of education The Language Fund of SCOLAR was established to support proficiency in the use of the Chinese (including spoken Putonghua) and English languages by the people of Hong Kong, ...

Ngày tải lên: 21/06/2014, 17:20

... removal of the second half of the PolC N-terminus would help to improve the results further Therefore, we took only the fragment of the multiple sequence alignment covering the first half of the PolC ... explore the structure of the PolC N-terminal region We found that this region includes two consecutive structural domains Both domains are distantly related to the structure of domain V of the clamp ... Interestingly, the first half of the PolC N-terminal region ( 1–100) also detected the PolIIIs-V domain, albeit weakly (hhsearch probability of 16%) The structures of PolIIIs-V and DnaA-I adopt a variant of...

Ngày tải lên: 05/03/2014, 23:20

... GM2 and the interaction of the GM2AP ⁄ GM2 complex with the enzyme To delineate in detail the separate effect of the introduced mutations on the lipid- and the enzyme-binding of this cofactor, ... that of the WT protein Only these variants of GM2AP were examined in all further studies To assess the potential of the variant GM2AP forms for the stimulation of HexA catalysed hydrolysis of GM2, ... nomenclature; they assign the number to S32, which represents the N-terminus of mature GM2AP.) This interaction might be crucial for stabilization of the position of the glycolipid within the spacious...

Ngày tải lên: 07/03/2014, 12:20

... region Effect of mutations in the C-terminal region of the RmInt1 IEP on RNA splicing in vivo We constructed a series of mutants to identify the functional features of the C-terminal region of ... with the high-affinity binding site in subdomain DIVa of the intron, whereas other regions of the RT and domain X interact with conserved catalytic core regions [12] Domain X is located in the ... DNA-binding region, an extension of domain X, or simply a nonfunctional extension In the present study, we investigated the C-terminal region of the RmInt1 IEP up to the maturase domain and the C-tail...

Ngày tải lên: 15/03/2014, 09:20

... selfimage of the scientific world as of the perception of society at large that visible effects of research in technological and finally economic terms are part of the matter Any concept of the law of ... Representatives of the Contracting Parties named in the preamble to the present treaty shall meet at the City of Canberra within two months after the date of entry into force of the treaty, and thereafter ... Consequently, the task of defining the term is part of the effort to design the subject matter itself There is no positivist approach or else given idea to the concept of international law of science...

Ngày tải lên: 16/03/2014, 00:20

... part of the coenzyme B-binding site Its unmethylated nitrogen atom Ne2 donates the shortest of the three hydrogen bonds of the protein to the phosphate group of coenzyme B The distance of ˚ 2.65 ... digestion of the a-subunit with trypsin, the generated peptides were analyzed by MALDI-TOF MS The peptide with a mass of 2446.36 Da (predicted to contain the thioglycine) was then analyzed by MALDI-TOF ... because the genes required for the biosynthesis of the nickel cofactor F430 [16] and for the post-translational modifications [9] are not yet known [17,18] Also, the in vitro reconstitution of active...

Ngày tải lên: 16/03/2014, 05:20

... structural changes in the myosin head The main goal of these studies was to understand the mechanism of these changes, i.e the mechanism of transmission of structural changes from the nucleotide- and ... accelerate the inactivation of the S1 ATPase upon mild heat treatment with the loss of the ability of nucleotides to protect the S1 against thermal denaturation [23] There is some discrepancy in the ... obtained by addition of 0.5 mM BeCl2 in the presence of mM NaF The formation of the complexes was controlled by measuring the K+-EDTA ATPase activity of the protein The ATPase activity of S1, t-S1, or...

Ngày tải lên: 17/03/2014, 10:20

... to +1157 relative to the AUG of the psbA mRNA (corresponding to the coding region of the C-terminal amino acids of D1 and the 3¢ UTR of the psbA mRNA) was amplified with the oligonucleotides psbA3/1 ... fractions of 500 lL; 10 lL of these fractions were used for UV cross-linking experiments In vitro synthesis of RNA and UV cross-linking of RNA with proteins Templates for the in vitro synthesis of psbD ... preferentially binds to the 5¢ UTR of the psbA message determined by an A-rich region eight nucleotides upstream of the AUG start codon To the best of our knowledge, this is the first RNA binding...

Ngày tải lên: 17/03/2014, 11:20

... the wild-type strain With L1, L4, LH2, HM or LH5, 50% to  80% of the cells had foci With LH3,  40% of the cells had foci LH1 and LH4 greatly hampered the formation of the foci and most of the ... Mutations on the flexible linker region of MukF affect the detachment of MukF C-WHD from engaged MukB heads For a biochemical assay of the detachment of MukF C-WHD from the MukB head, we employed the MukE–MukF(M+C)–MukBhd ... of the symmetric dimer was observed (Fig 3A; the band labeled as ‘sym’), apparently showing that they affect the detachment of the MukF C-WHD from engaged MukB heads The band intensities of the...

Ngày tải lên: 23/03/2014, 05:22

... testing the hypothesis that the N-terminal regions of TTR affect the affinities of TTR for thyroid hormones A previous study revealed that altering the structure of the N-terminal region influenced the ... primary structure of the N-terminal regions of TTR and the biological function of TTR 4014 Results Synthesis of recombinant human TTR In the crocodile, TTR is only synthesized in the liver during ... Examination of the alignment of TTR amino acid sequences from 20 vertebrate species revealed that the region of the subunit which changed in a distinct and directed manner was the N-terminal region The...

Ngày tải lên: 23/03/2014, 10:21

... of the TjZNT2 N-terminus maintained its ability to complement the mutant Exchange of the TM III–IV loop (L region) , one of the least similar regions between the two proteins, did not affect the ... with the exception of the N-terminus and the TM III–IV loop To examine the functions of TjZNT1 and TjZNT2, these transporters were tagged with hemagglutinin (HA) at their N-termini to verify their ... that is not present in the sequence of TjZNT1 The present work provides several pieces of evidence for the involvement of this N-terminal region in the ion selectivity of TjZNT2 and related proteins...

Ngày tải lên: 29/03/2014, 00:20

... expressing the recombinant mPC1 ⁄ 3, the presence of the 87 kDa form in excess of the 66 ⁄ 71 kDa facilitates isolation of the enzyme and helps in maintaining the enzymatic activity at a proper level The ... However, the proregion is a potent inhibitor of PC1 ⁄ and binds the active site with nm affinity in vitro resulting in the formation of a stable proregion–enzyme complex [5] Additional cleavage of the ... liter of bacterial culture Effect of the CT-peptide on mPC1 ⁄ enzymatic activity The effect of various concentrations of the purified CT-peptide on the cleavage of the fluorogenic substrate pERTKR–MCA...

Ngày tải lên: 30/03/2014, 08:20

... directly the role of CHD3 in the regulation of PS1 gene in vivo We first 1440 Fig Effect of N-terminal deletions of CHD3 on the inhibition of transcription by CHD3 and on the binding of CHD3 with ... several of these corepressors also interact with the C-terminal regions of CHD3 downstream of residue 1676 [46] or the conserved regions of CHD4 and dMi2 [50] Models proposed to explain the repression ... sequence with the central consensus 5¢-GGAA ⁄ T-3 (Fig 1A) [18,20] Based on the sequence homology of the ETS domain, and the conservation of other functional domains, a phylogenic tree of the ETS gene...

Ngày tải lên: 30/03/2014, 09:20

... excess of a nonspecific RNA, the c-myc coding region, had no effect on binding (results not shown) These data indicate that the 36 kDa protein bound to the 194–280 region of the 3¢UTR These observations ... localization of mRNAs such as b-actin and MBP Here we describe the specific binding of a trans-acting factor to the region of the 3¢UTR of c-myc mRNA previously shown to contain the localization ... proteins and one other minor component Comparison of the mobility of these proteins in SDS ⁄ PAGE with that of molecular mass standards indicated that the major proteins were of approximate molecular...

Ngày tải lên: 30/03/2014, 15:20

... at early NGG codons is the reason for the observed low gene expression The length of the peptidyl moiety of the peptidyltRNA influences the drop-off process in the case of very small mini-genes ... inhibit growth of the strain even in the presence of IPTG induction All of these results suggest that the low gene expression associated with NGG codons in the downstream region following the initiation ... different from the related codon AGG The other NGG codons CGG and UGG were not analysed for the influence of increased concentration of cognate tRNAs The question remains whether the plasmid associated...

Ngày tải lên: 30/03/2014, 20:20

... perfectly match their theoretical values, assuming formation of all seven disulfide bonds in the sPLA2 molecule Influence of the mutations on the secondary structure and overall conformation of the AtnI2 ... demonstrated the importance for neurotoxicity of the C-terminal region stretching over the top of the Atx molecule (Fig 2), particularly certain hydrophobic/aromatic and basic residues [21–23] The same region ... in the Cterminal, AtxA-like region As shown by the studies of the AtxA double mutant (K108N/K111N) [21,22], the influence of K108N substitution on neurotoxicity and protein-binding properties of...

Ngày tải lên: 31/03/2014, 08:20

... number of the diverse superfamily of GPCRs, not least among the prostanoid receptor subfamily of GPCRs [53] The EP3 subtype of the PGE2 receptors is one of the most heavily spliced among all of the ... by either the presence of a TI site for the TPb mRNA transcript at )12 or due splicing of nucleotides elsewhere in the 5¢ flanking region of the TP gene to nucleotides located within the )12 region ... the current study, we sought to investigate the molecular basis of the differential expression of TPa and TPb isoforms Our initial aim was to map the patterns of exon usage in the 5¢ UTR of the...

Ngày tải lên: 31/03/2014, 09:20

... deletions investigated, with the exception of deletion R30hE5, increased the number of colonies and the colony size of keratinocytes growing in soft agar The effect of the empty vector on colony ... The results shown in this communication demonstrate that the expression of theHPV16 E5 protein increases the number and the size of HaCaT cell colonies growing in soft-agar The results were the ... but determines the number of cells and therefore the colony size (see Fig 6) The data here detailed represent the first quantitative description of the ability of HPV16 E5 to Page of 10 (page number...

Ngày tải lên: 20/06/2014, 01:20

... to the reliability of the House Finance Office’s Statement of Accountability and the effectiveness of internal controls The computer system serving the Office of Finance is maintained by the ... disbursements of the Director, House Office of Non-Legislative and Financial Services, for appropriated and other funds arising from cash transactions of the House Office of Finance for the fiscal ... Other Funds arising from cash transactions of the Office of Finance, House of Representatives, for the fiscal year ended September 30, 1993 The House Office of Finance performed receipt and disbursement...

Ngày tải lên: 20/06/2014, 06:20