the role of actin in the cell

... representatives of the evolution of the surgical management of small cell lung cancer up to the present Surgery Revived The role of surgery was revaluated after the introduction of the TNM staging system The ... were denied of the benefit that radical resection can offer in long term survival Therefore the role of surgery in the integrated management of small cell lung cancer remained under investigation ... form of protection of the bronchial stump should be carried out to minimize the risks for stump insufficiency [27-29] answers regarding the precise role of surgery according the stage of the disease...

Ngày tải lên: 10/08/2014, 10:20

... analyzed the role of gangliosides in the association of ErbB2 with lipid rafts in mammary epithelial HC11 cells The key role played by gangliosides in defining the distribution of ErbB2 into signaling ... fractions in HC11 cells Because in cells coexpressing both ErbB2 and EGFR, as is the case of HC11 cells, the two proteins strictly interact and EGF preferentially stimulates the formation of ErbB2 ... specifically interact with the purified recombinant extracellular domain of EGFR [22] and that this tyrosine kinase receptor contains a structural domain with targeting information for lipid domains [23]...

Ngày tải lên: 23/03/2014, 10:21

... on the regulation of the IP-10 secretion The aim was to investigate the pathways of IP10 secretion in a in vitro system including the cell types most likely involved in the IP-10 secretion in the ... distinct soluble factors governing the IP-10 response in endothelial versus epithelial cells The secreted factor from lung epithelial cells might be a growth factor, interleukin or interferon, since ... studies To further probe the signalling pathways involved in modulating IP-10 expression in the epithelial cell/ PBMC co-cultures, we investigated the pharmacological effect of a number of signal...

Ngày tải lên: 12/08/2014, 15:21

... apoptosis in endothelium of intestine, lung, fat and thymus Interestingly, the apoptosis was inhibited in the endothelium by administration of TNF-binding protein, a protective factor against LPS-induced ... in the de novo ceramide synthesis pathway in rat lung epithelial cells 84 Figure Effect of CS exposure on the activity of enzymes in the sphingomyelinase pathway in rat lung epithelial cells ... endothelial and epithelial cells in vitro Overproduction and accumulation of ceramides are involved in the alterations of the lung parenchyma induced by CS, which culminate in the development of...

Ngày tải lên: 24/08/2014, 09:42

... cell in a state of dormancy or inactivity in which the cell is not dividing, therefore is in G0 phase of the cell cycle Quiescence should not be misinterpreted with other processes in which cells ... 1 AN INVESTIGATION INTO THE ROLE OF CHEMOKINES IN HAEMOPOIETIC STEM CELL QUIESCENCE Amy Sinclair BSc (hons), MRes Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy ... conclusions In order to gain a deeper understanding of the biological function of chemokine signalling in HSC biology, we used an in vivo murine system First, we examined mRNA transcripts of CXC chemokines...

Ngày tải lên: 22/12/2014, 21:11

... Evaluation of Human SPHK Inhibitors visible color change Within the linear range of the assay (~5-25μg/mL), the more protein present, the more the Coomassie Blue binds changing the absorbance of the ... of Human SPHK Inhibitors components in the samples, which are then separated in the TLC plate The plate was run in the chamber until the solvent front reached 2cm from the top of the plate, then ... was not in the final list and not being tested further, is mainly because the yield of compound 2a3 is really low (~7%) Therefore, obtaining compound 3a3 requied a large amount of the starting material-Garner’s...

Ngày tải lên: 11/09/2015, 16:06

... Staining 3.1.3.1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the mineralization ... to be the best inhibitor candidate, in terms of the ability to penetrate into the cells and inhibit the endogenous SPHK1 and the good yield obtained from the chemical synthesis procedure Therefore, ... vs cock) However, in cells cultured in the presence of 1μM of DMS, 1μM of CP6, and 5μM of CP6, there was no increase in osteocalcin expression, compared the cells grown without inhibitors (cock+1...

Ngày tải lên: 11/09/2015, 16:06

... were counted The percentages of the expressed cell surface markers in the cells are summarized in Table 4.1, and plots of receptor expression of the last passage cells cultured in two different ... for a total of 28 days, then fixed and stained with Alizarin Red S The Alizarin Red S Staining quantification results are shown in Figure 4.11 Alizarin Red Staining quantification of human BM-MSCs ... the presence of S1P did not show any side-effect on maintaining stemness of the cells However, the amount of cells that were proliferated in the S1P serum-free, or S1PPDGF-AB combined culture...

Ngày tải lên: 11/09/2015, 16:06

... procedure of extracting these cells is much less painful, compared with extracting MSCs from BM This study investigated the function of S1P, S1P+10%FBS, S1P+1%FBS and PDGF-AB in human AD-MSCs and the ... transplanting them into the body, by committing stem cells along specific differentiation path More work, including in vivo work, needs to be done to fully validate the potential for SPHK inhibitors in ... osteopenia, where the bone volume is decreased but the adipose tissue increases (Jaiswal et al., 2000) It also suggests the potential for the use of SPHK inhibitors in preconditioning stem cells before...

Ngày tải lên: 11/09/2015, 16:06

... et al (1998) “N,N-Dimethylsphingosine Is a Potent Competitive Inhibitor of Sphingosine Kinase but Not of Protein Kinase C: Modulation of Cellular Levels of Sphingosine 1-Phosphate and Ceramide.” ... “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other Lipid Mediators 86(1-4):18-25 ... "Signalling mechanisms in sphingosine 1-phosphatepromoted mesangial cell proliferation." Genes Cells 7(12): 1217-30 Kee, T H., P Vit, et al (2005) "Sphingosine kinase signalling in immune cells." Clin...

Ngày tải lên: 11/09/2015, 16:06

... increasing need for more MSCs is one of the main motivations of the present study, in particular, in studying the role of S1P in adult stem cell proliferation Another major motivation is the pressing ... activation, while HDL could inhibit all of these, by inhibiting the SPHK activity triggered by TNFα These findings suggested an atherogenic role for SPHK activation Interestingly, Nofer et al (2004) reported ... stimulate their growth and maintain of their multipotency, which limits the development of the strategies for stem cell expansion in better controlled, serum-free, conditions In this study, the role of...

Ngày tải lên: 11/09/2015, 16:06

... it finds putative microRNA binding sites in the sequence of interest before identifying the targeting microRNA In a luciferase reporter screen, average repressions of 30% or more for 168 of 226 ... differentiate into all cell types in the body Although the clinical use of adult stem cells, which are present in multiple tissues in the mammalian body, is attractive due to the lack of allogenecity, ... Maintaining Pluripotency: LIF, BMP & Wnt Signalling One of the major breakthroughs in ESC maintenance was reported in 1988, when leukemia inhibitory factor (LIF), a member of the IL-6 cytokine...

Ngày tải lên: 12/09/2015, 08:16

... cells in the cell cycle and promote cell proliferation However, the function of Diva in the regulation of cell cycle has never been investigated In the present study, the effects of Diva on the ... by the specificity of the probes The contradiction lies in the expression of Diva in the adult testis and the E15 17 embryo between these two papers, indicating that the expression of Diva in ... known as the key players in the control of apoptosis, their overexpression in cell lines or in transgenic animals revealed that they are also involved in the control of cell proliferation (Linette...

Ngày tải lên: 12/09/2015, 08:19

... effective interplay of the both arms in the immune system 1.2 Innate Immunity 1.2.1 Overview of Innate Immunity The innate immunity is known as the front line of host defence against invading pathogens ... with distinct but unique functional properties Nevertheless, the cells and molecules are able to act in concert to maintain the integrity of the immune system Cells of the immune system originate ... in innate immunity These cells include epithelial cells, eosiniphils, DCs, mast cells, phagocytic cells, NK cells and γδ T cells The natural flora and fauna found in the host’s body also form the...

Ngày tải lên: 16/10/2015, 11:59

... profitability is declining The internalization of information within the same institution may thus enhance intra-circuit and inter-circuit stability by reducing the incentive to overfinancing The ... Creating a stronger investor base amounts to strengthening the circuit by completing the financial market structure of the economy, thus eliminating discontinuities in the transmission of information ... placing money with investment financial institutions in the capital market, and hold the remaining share in noninterest bearing, short-term bank deposits (eq 7) The share of savings going to the...

Ngày tải lên: 24/10/2012, 09:33

... English at the expense of the other languages that exist in the country, the adult language program in Botswana maintains the hegemony and the gap between the poor and the rich, the major and minority ... for the betterment of their conditions It could mobilize the so called minorities to oppose their status and challenge the hegemony of the majority communities in Botswana 31 In most parts of the ... is for the most part the preserve of the few (elites) The choice of which language or dialect to use to teach (medium of instruction) reflects the interests of those elite In countries in which...

Ngày tải lên: 05/11/2012, 16:27

... categories of business-to-business advertising are industrial and professional advertising b.1 Industrial advertising Advertising targeted at individuals who buy or influence the purchase of industrial ... development of the industry also fosters the other industries in society, which contributes to the development of the economy as a whole In Vietnam, spending for advertising is rather lower than that in ... advertising industry Since the opening policy and law of foreign investment, advertising industry has seen remarkable changes The growth rate of advertising service is the highest in economy, in which...

Ngày tải lên: 16/04/2013, 11:05

... establishment of the Faculty of Arts and the Faculty of Medicine in 1935, the Faculty of Economics and Law in 1936, the Faculty of Theology in 1942 and, finally, the Faculty of Science in 1954 The main ... the Folketing, on the role of the University as the principal shareholder in Cheminova The listing of Cheminova on the Stock Exchange in 1986 and the establishment of the first science park in ... behind the economic upswing and increase in employment in Denmark since the start of the economic boom at the beginning of 1994 The Greater Copenhagen area together with the Municipalities of...

Ngày tải lên: 16/01/2014, 16:33

... critical in ensuring the effective use of ICTs in the health sector Programme designs should consider the role of the intermediary at the same time as, if not before, the role of the technology The ... reminding them about their medication The messages can be sent in one of three languages and in a range of styles to ensure they not become boring Of the 138 patients involved in the pilot, there ... that ‘there has been virtually no progress in making the internet available in the least developed countries, especially in the rural areas’ By including the rural population in the group of beneficiaries...

Ngày tải lên: 14/02/2014, 09:20

... president of the National Academy of Engineering The Institute of Medicine was established in 1970 by the National Academy of Sciences to secure the services of eminent members of appropriate professions ... Engineering in providing services to the government, the public, and the scientific and engineering communities The Council is administered jointly by both Academies and the Institute of Medicine ... by the Governing Board of the National Research Council, whose members are drawn from the councils of the National Academy of Sciences, the National Academy of Engineering, and the Institute of...

Ngày tải lên: 15/02/2014, 16:20