the interaction of organic matter with pesticides
Báo cáo khoa học: A spectroscopic study of the interaction of isoflavones with human serum albumin pdf
... energetics The effect of temperature on the interaction of genistein with HSA has been followed in the range 17–47 °C The binding constant, K, exhibits a recipro- Fig Quantitation of the interaction of ... overlap of the emission spectrum of the donor with the absorption spectrum of the acceptor The energy transfer efficiency E is related to the distance (r0) between acceptor and donor, and also to the ... constant decreased with increasing ionic strength (Fig 5B), establishing the role of ionic interaction in the binding The Stokes radius of HSA in the presence of increasing concentrations of potassium...
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Báo cáo Y học: Tryptophan fluorescence study of the interaction of penetratin peptides with model membranes pdf
... internalization [12] The aim of this study was to gain better insight into the mode of interaction of the penetratin peptide with lipid bilayers and to investigate the role of the Trp residues and the lipids ... from the quenching of the intrinsic Trp uorescence intensity of the peptides, upon addition of SUVs The uorescence intensity at 350 nm, expressed as the percentage of the uorescence of the lipid-free ... decrease of the mean lifetime of the Trp residue(s) in the W56F- and WT- or W48F-penetratin The decrease of the mean Trp lifetime for the three peptides might account for the decrease of the Trp...
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Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 1
... formed upon interaction with micelles The region which is rich in tryptophan in the middle of the peptides interacts with one layer of the membrane and the two termini are oriented toward the aqueous ... role in the activity of antimicrobial peptides The one cysteine of the S3 peptide which is derived from the Sushi domain of Factor C allows the formation of S3 dimers The S3 dimer is capable of disrupting ... is also related to the L- or D- enantiomer However the effect of enantiomer of the amino acid is dependent on whether the replacement affects the structure of the peptide and the amphipathicity...
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Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 3
... value The presence of V4 further increased the compressibility of the monolayer, indicative of the increased molecular area, which was consistent with the result of excess surface pressure 154 The ... disruption The direct observation of the kinetics of these processes would greatly enhance the understanding of the mechanism of V4membrane interactions In the future, a possible method to address these ... They have the same hydrophobic alkyl chains and differ in the headgroup The molecular packing of the monolayer is mainly dependent on the hydrophobic interaction between the alkyl chains of the...
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Investigation of the interaction of antimicrobial peptides with lipids and lipid membranes 2
... amplitude of the ACF is inversely proportional to the particle number N However, if the fluorescence yields of the two species are not the same, the fluorescence yield of the particles will affect the ... overlapped with that of labeled DPPG LUVs When the peptide/lipid ratio increased to 1:20, the amplitude of the ACF rose and the diffusion time increased compared with DPPG vesicles The amplitude of the ... detected in the two solutions, the proposed impurity of free TMR in V4-TMR solution would be 0.18%, which is well within the limits of the manufacturer The intensity of V4-TMR on the coverslip...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... as a consequence of the mutagenic replacements Effects of the His120Asn and His145Asn mutations on the affinity of metal binding to arginase II To further examine the effects of the His120fiAsn and ... the interactions of arginase II with l-arginine and agmatine are greatly altered by replacement of this residue with aspartate To the best of our knowledge, this is the first report in which the ... being involved in the stabilization of the transition state [27], but not in the stabilization of the substrate in the Michaelis–Menten complex [24] Also in agreement with this, the Km value was...
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Báo cáo Y học: Molecular interaction of neutral trehalase with other enzymes of trehalose metabolism in the fission yeast Schizosaccharomyces pombe pdf
... Taken together, these results support the idea that in S pombe the regulation of Ntp1p activity under stress relies on the existence of some kind of interaction between Ntp1p and Tps1p, or other elements, ... allow the construction of vectors with Ha6H tag fusions at the C-terminus The resulting plasmids were digested at the unique SfiI site within the ntp1+ coding region (at position 1665) and the linear ... than the osmotically induced activation [17,23] In the latter case, the Pka/Sck1p-mediated activation of Ntp1p appears to occur at the level of the enzyme synthesized de novo [17] Finally, the...
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Báo cáo khoa học: Interaction of caspase-3 with the cyclic GMP binding cyclic GMP specific phosphodiesterase (PDE5a1) potx
... treatment of the cells with the caspase-3/7 inhibitor, Ac-DEVD-CHO (added at the time of transfection with caspase-3 plasmid construct) Overexpression of PDE5A1 did not inhibit the auto-activation of ... agreement with studies by Turko et al [14], who reported identical changes in the kinetic constants of the D781A mutant The DQGD site is within the boundary of the catalytic domain of PDE5A1 ... role in the binding of competitive PDE4 inhibitors [36] The side chain of this residue may conceivably p-stack with the purine base of the bound substrate and form hydrophobic interactions with...
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Báo cáo Y học: Biophysical characterization of the interaction of high-density lipoprotein (HDL) with endotoxins doc
... necrosis factor-a production (Fig 10) The comparison of the results of the interaction of LPS with HDL to those published for the interaction of the former with another serum protein, albumin, indicates ... ratio of [LPS]/ [HDL] : (Fig 3) indicates the significant influence of the acyl chain moiety of LPS in the interaction which might be connected with the observation that the acyl chain melting in the ... model of the LPS– HDL interaction can be deduced The binding of HDL takes place essentially to the diglucosamine sugar backbone and the 4¢-phosphate of lipid A The binding-places within the HDL...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... interacting with the protein, because the resonance signals of the glucose residue overlapping with those of the galactose residue within the lactose affected the subtraction of the free induction ... interacts with the region of H1 and the O-methyl group The crystal structure of EW29Ch with lactose showed that the O2, O3 and O4 atoms of the galactose residue of lactose formed hydrogen bonds with ... to that of the higher binding site by NMR, even if the sugar-binding ability of one of the two binding sites is much higher than that of the other However, the hemagglutinating activity of lectin...
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Báo cáo khoa học: The influence of cholesterol on the interaction of HIV gp41 membrane proximal region-derived peptides with lipid bilayers potx
... by the particular disposition of key residues in the context of long peptides or even in the context of the full protein Bearing this in mind, a MPRP-S role in the interaction of the MPR with ... about the interaction of peptides with large unilamellar vesicles (LUV) The fluorescence quantum yield is dependent on the polarity of the microenvironment of the Trp residues, as well as on the ... Castanho The membrane interaction behaviour of MPRP-C and MPRP-I is independent of the membrane phase but not of the presence of charged lipids Cholesterol does not reduce the extent of or potentiate...
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Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA ppt
... towards the 3¢ end of the top strand and the 5¢ end of the bottom strand (Fig 4B) This agrees with the presence of a larger residue that makes stable contacts with this region of DNA The interaction ... achieved The combination of these experiments with the footprinting results and previous 197 KNOX homeodomain–DNA interactions A B Fig A model for the interaction of HAKN1 with DNA (A) Diagram of the ... least one of the members of the CÆG pair at the fourth position of TGAC in the HAKN1–DNA complex The two leftmost positions of the core interact through the minor groove with the N-terminal arm in...
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Báo cáo khoa học: The interaction of the Escherichia coli protein SlyD with nickel ions illuminates the mechanism of regulation of its peptidyl-prolyl isomerase activity ppt
... cluster that is the core of the domain The IF domain of SlyD is aligned with the IF domain of the archaeal homologous MtFKBP17 [32] (Fig 3) The main difference between these two domains is the longer ... hydrophobic interactions with the a1-helix, the b2-strand and the b3-strand, but it also establishes a few contacts with the interconnecting segments The structure of the PPIase module of SlyD closely ... helix connects to the rest of the domain through a well-dened segment extending from the b3-strand, and is positioned at the convex side of the b-sheet near to the ends of the b4-strand and b5b-strand...
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Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc
... characteristic of the monoformazan, i.e the fully reduced product of INT [15,17] In the following aeration of the medium, the absorbance of the spectral bands decreased, but the shape of the spectrum ... inward The rates of oxygen uptake were deduced from the slopes of the tangents to the oxygraphic traces, and the contact points of the tangents with the curves were used to determine the O2 concentrations ... [13], either by measurement of the rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of reduction of INT into formazan in the presence of superoxide...
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Báo cáo y học: "HIV-1 integrase modulates the interaction of the HIV-1 cellular cofactor LEDGF/p75 with chromati" pptx
... [33] The surface of interaction of the IBD with a subset of these proteins partially overlaps with that of integrase (reviewed in [19]) The affinity of IBD for integrase is higher than for some of ... accumulate in the cytoplasmic compartment of the transfected mouse fibroblasts as a result of the saturation of the nuclear import pathway responsible for the nuclear localization of these mutants ... chromatin and with HIV-1 integrase [1,2] To determine the effect of the ionic strength on the interaction of HIV-1 integrase with chromatin bound LEDGF/p75, we evaluated the consequence of increasing...
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Báo cáo y học: "Highly specific inhibition of leukaemia virus membrane fusion by interaction of peptide antagonists with a conserved region of the coiled coil of envelope" potx
... alignment of the adjacent peptide sequences relative to the target-binding site on the coiled coil A recurring theme in the interaction of the C-terminal helix of the trimer -of- hairpins with the coiled ... conserved amino acids of the LHR are located on the face of the LHR that interacts with the grooves on the coiled coil By examining the location of substituted residues on the HTLV-1 TM it becomes ... unfavourable charge interaction would improve the binding of the peptide to the BLV coiled coil and thereby improve the inhibitory activity of the peptide We therefore synthesized a peptide,...
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Characterization of the interaction of EEN and its domains with ca2+ and proline rich domain
... the dimer by the FPLC because of the large molecular weight (Figure 4.1.3) In further, these polymers may accelerate the process of the EEN aggregation Therefore preventing the formation of the ... objectives of this research are explained, followed by the organization of the thesis Chapter gives a literature review on the subject matter of this study as well as the background for other research ... Chapter presents the results obtained, while Chapter discusses the contribution of these results on understanding of the possible role of the random coil PRD and the influence of Ca2+ on EEN in...
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Interaction of burkholderia pseudomallei with cells of the immune system
... production The flagellae of many motile bacteria e.g Salmonella, are comprised of the basal body, the hook, and the filament that is composed mainly of the protein FliC (Macnab, 2004) The secretion of ... with the light microscope under high magnification to check for the absence of bacteria swimming among the cells in the medium Infection of T cells with live B pseudomallei in the absence of a ... facilitate therapeutic developments Our objective is to gain insights into the pathogenesis of the bacteria by employing the use of in vitro culture systems to study the interactions of B pseudomallei...
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EFFECT OF CARBON TO NITROGEN RATIO ON THE COMPOSTING OF CASSAVA PULP WITH SWINE MANURE
... was poor when the initial C/N ratio was low There was no difference in the changes of C/N ratio in the compost pile with the same initial C/N ratio with or without p.d (Figure 8) These results ... 2) The pH of compost in piles and were lower than the other piles This may be due to the higher organic carbon content (around 45%) in these piles A large pH drop in the compost piles at the ... the first day of composting, total K in pile and were lower than those of the other piles may be due to the lower amount of swine manure added to these piles to obtain higher initial C/N At the...
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