structural characterization of drug dna complexes

... bulk of the phenyl rings discourages both dimerization and formation of the homoleptic [Fe(TIP)2]2+ complexes Recently, we described the synthesis and structural characterization of a series of ... Chemistry, Department of 4-2012 Synthesis and Structural Characterization of Iron(II) Complexes with Tris(imidazolyl)phosphane Ligands: A Platform for Modeling the 3-Histidine Facial Triad of Nonheme Iron ... described the synthesis and X‐ray structural characterization of iron(II) complexes supported by tris(imidazolyl)phosphane ligands (2‐TIPPh2 and 4‐TIPPh) Three of the complexes – [1](OTf)2, [2]OTf,

Ngày tải lên: 22/10/2022, 21:07

... pharmacology of benzimidazoles are of interest in medicinal chemistry because of their ability to interact with a range of different enzymes and receptors 3,4 Transition-metal complexes of benzimidazoles ... NMR spectra of the cobalt and iron complexes could not be recorded due to their paramagnetic properties Because of the paramagnetic properties of the iron atom, H NMR spectra of iron complexes ... reports of benzimidazole transition metal complexes Some consist of 2-substituted benzimidazoles and the others consist of benzimidazole-based mixed ligands 23−25 We have reported on the structural

Ngày tải lên: 12/01/2022, 23:28

... disproportionation of the FexO particles into magnetite and R-Fe Structural characterization of the Wu¨stite nanocrystals prepared by these methods reveals incorporated small seeds of magnetite Self-assembly of ... precipitations of diluted hexane solutions with an equal volume of acetone Decomposition of Iron Pentacarbonyl in the Presence of Pyridine N-Oxide In a typical reaction, 7.6 mmol of PyO and 3.02 mmol of ... particles of 14 nm and spherical particles of nm obtained by decomposition of mmol Fe(OAc)2 in 12 mmol of OA/14 mL of TOA (c) Cubic FexO particle isolated in the early growth state (8 mmol of Fe(OAc)2

Ngày tải lên: 01/12/2016, 16:32

... HÀN LÂM KHOA HỌC VÀ CÔNG NGHỆ VIỆT NAM VIỆN CÔNG NGHỆ SINH HỌC NGUYỄN HỒNG THANH STRUCTURAL CHARACTERIZATION OF ANIMAL ADENOVIRUS FIBRE HEADS Chuyên ngành : Hóa sinh học Mã số : 62 42 01 16 TÓM ... gen phù hợp với mục đích điều trị bệnh hiểm nghèo liệu pháp gen Do thực đề tài: ? ?Structural characterization of animal adenovirus fibre heads” (tên tiếng Việt: Nghiên cứu cấu trúc protein sợi ... Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes, and the first adenovirus with either one short or three long fibers per penton Journal of Virology

Ngày tải lên: 02/09/2021, 10:53

... release of Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, Singapore 2Department of Biological ... these two classes of antiviral drugs has occurred in the circulating strains of the IAVs10, there is an urgent need to develop new therapeutic approaches Non -structural protein (NS1) of IAV is a potent ... Published: 16 September 2016 Biochemical and structural characterization of the interface mediating interaction between the influenza A virus non -structural protein-1 and a monoclonal antibody

Ngày tải lên: 19/11/2022, 11:42

... detected in those of larch Following pretreatment, the frequencies of β–β′ , β-5, and γ-ester in the LCCs of larch were found to be higher than in those of yellow poplar The efficiencies of enzymatic ... the chemical com­ positions of softwood and hardwood The yield of LCCs decreased after Fenton oxidation-hydrothermal treatment, while that of LCC2 was higher than that of LCC3 Therefore, the hemicellulose ... structures of the yellow poplar Meanwhile, β–β′ and β-5 linkages of lignin were detected in the LCC3 of larch (Fig 6) The LCCs of yellow poplar contained glucan and lignin as most of the hemicellulose

Ngày tải lên: 01/01/2023, 12:23

... VIỆNHÀNLÂMKHOA HỌC VÀ CÔNGNGHỆVIỆTNAM VIỆN CÔNGNGHỆSINHHỌC NGUYỄNHỒNG THANH STRUCTURAL CHARACTERIZATION OFANIMALADENOVIRUSFIBRE HEADS Chunngành: Hóasinh họcMãsố :62 4201 16 TĨM TẮTLUẬNÁNTIẾNSĨSINHHỌC ... chuyển gen phù hợp với mục đích điều trị bệnhhiểm nghèo liệu pháp gen Do thực đề tài: “Structuralcharacterization ofanimaladenovirusfibre heads” (têntiếngViệt:N g h i ê n cứu cấu trúc protein sợi ... triểndựatrênhệgiencủaadenovirustype5lâynhiễmtrênngƣời(thuộcchiMastadenovi rus) Một số đƣợc nghiên cứu dựa hệ gien ovine adenovirus7 (Hofmann 1999; Kümin 2002; Both 2004) Tuy nhiên, hiệu sử dụng cácvectorcónguồngốctừadenoviruslâynhiễmtrênngƣờibộclộcáchạnchếnhƣ:

Ngày tải lên: 30/08/2023, 21:27

... STRUCTURAL CHARACTERIZATION AND BIOCHEMICAL ANALYSIS OF ID2, AN INHIBITOR OF DNA- BINDING MARIE VIVIAN WONG TZU YENN (B.Sc.), University of Melbourne A THESIS SUBIMTTED FOR THE DEGREE OF PHILOSOPHY ... experience ! i! ! TABLE OF CONTENTS TABLE OF CONTENTS .ii! SUMMARY v! LIST OF TABLES vi! LIST OF FIGURES vii! LIST OF SYMBOLS .xi! ... the E-box motif (CANNTG) of enhancer regions of a number of genes to activate their transcription One such example was the enhancer region of the heavy and light chain of the immunoglobulin genes

Ngày tải lên: 09/09/2015, 10:20

... between 1.5 – 2 mg of pure ID2 per litre of bacterial culture The chromatography profiles of all 3 constructs were virtually identical so a representative set of profiles is shown in Figure ... culture. The chromatography profiles of all constructs were virtually identical so a representative set of profiles is shown in Figure (A, C, E). The affinity chromatography profile utilizing the His6 ... inhibition of an E-protein transcribed gene, the cyclin dependent kinase (CDK) inhibitor, p21. Downregulation of p21 caused a cascade of signaling events that ultimately led to the activation of genes

Ngày tải lên: 09/09/2015, 10:20

... "A natural classification of the basic helixloop-helix class of transcription factors", Proceedings of the National Academy of Sciences of the United States of America, 94 , 5172-6... ... loss of binding of a Group A bHLH transcription factor to its cy5-labeled DNA probe with an increasing concentration of ID2. E47, a Group A bHLH transcription factor and known binding partner of ... M. and Berglund, H. (2010) "Structural determination of functional domains in early B-cell factor (EBF) family of transcription factors reveals similarities to Rel DNA- binding proteins and a novel

Ngày tải lên: 09/09/2015, 10:20

... process) Loss of hydrated H2 O Removal of coordinated H2 O Elimination of coordinated acetate groups Complete decomposition of the complex and formation of metal oxide as a final product Loss of hydrated ... loss of lattice water from the complexes The weight loss at 200 ◦ C is due to loss of coordinated water The weight loss at 300 ◦ C can be attributed to the removal of acetate groups The weight of ... formulation of the complexes with a 2:1 metal to ligand ratio and octahedral geometry The molar conductance values of the UO (II) -complexes revealed their nonionic character The ligands and their complexes

Ngày tải lên: 12/01/2022, 23:32

... ionic strength of the solvent environment and ⁄ or presence of small organic compounds (dioxane and MPD) seems to favor formation of dodecahedra over isolated pentamers. Previous structural analysis of the ... bind- ing of minifiber. The fluorescence emission of the TMR was assumed to be independent of the binding of the minifiber to the penton base, because it is attached at the C-terminal extremity of the ... of the protein solution at a concentra- tion of 5–10 mgÆmL )1 was added to 1 lL of well solution. Small crystals grew within 2–3 months, reaching dimensions of 50 · 50 · 50 lm. Microseeding of

Ngày tải lên: 19/02/2014, 06:20

... Purification profile of Cbl-TKB 49 Fig 2.2 Gel filtration profile of cleaved Cbl-TKB 49 Fig 2.3 Glutaraldehyde cross-linking of Cbl-TKB 50 Fig 2.4 Initial crystals from the screening of Cbl-TKB complexed ... N Number of binding sites Nav Voltage gated sodium channel isoform Nav1.4IQ IQ motif of voltage gated sodium channel isoform 1.4 Nav1.6IQ IQ motif of voltage gated sodium channel isoform 1.6 ... PCR amplification of CaM and ΔNav1.6 cDNAs 88 Fig 3.2 Colony PCR of successfully ligated and transformed CaM and ΔNav1.6 clones 89 Fig 3.3 12.5% SDS-PAGE purification profile of His-ΔNav1.6 90...

Ngày tải lên: 11/09/2015, 09:11

... region of VpU, as indicated by a small number of observed long-range NOEs [24] NMR structure of micelle-associated HIV-1 VpUcyt Another important aspect is the topology of the cytoplasmic domain of ... the expected number of cross-peaks of VpUcyt were obtained with perdeuterated DPC (DPC-d38) at 30 °C A series of HSQC spectra of mm VpUcyt was recorded with varying amounts of DPC-d38 in the sample ... mark the theoretical average values of the downfield shift of 13Ca (top) and 13CO (bottom) as well as of the upfield shift of 1Ha resonances (middle) characteristic of a 100% helical secondary structure...

Ngày tải lên: 18/02/2014, 06:20

... Nevertheless, the cDNA structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 residues, ... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... trifluoroacetic acid cDNA cloning The cDNA of conomarphin was obtained unexpectedly when the cDNA cloning of M-superfamily conotoxins was performed from cDNAs reverse transcribed from total RNAs of the venom...

Ngày tải lên: 18/02/2014, 17:20

... others A structural alignment of these three models shows that they are indeed close structural Table The quality of fits (described by the chi-squared per degrees of freedom) for a series of Ca2+ ... degree of conformational flexibility in the two EF hand motifs of each CaM domain The presence of a relatively large number of Ca2+⁄ CaM ⁄ peptide complexes in the PDB opens up the possibility of ... 1.2 molar ratio of Ca2+ ⁄ CaM to PhK5 had been reached The inset shows an expanded view of the indicated area of the spectrum (Bottom) An overlay of spectra from the titration of Ca2+ ⁄ CaM with...

Ngày tải lên: 19/02/2014, 17:20

... Ó FEBS 2003 Minor isomer of the Hex3 glycoform (estimated from the intensity of the fragments in b a Major isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS2 experiments) ... to the phosphate group at O-4 of Kdo [11,13,16] Structure of the core region of the Hep3-glycoforms In the 1H NMR spectrum of OS-1 (Fig 5A), anomeric resonances of HepI–HepIII were observed at ... Biochem 270) Preparation of OS material O-Deacylation of LPS O-Deacylation of LPS was achieved with anhydrous hydrazine, as described previously [16,26] Fig Structural model of the lipopolysaccharide...

Ngày tải lên: 08/03/2014, 02:21

... introduction of each single mutation Expression studies Ten micrograms of wild-type or mutant cDNA expression vectors were introduced into 1.6 ã 106 of human HEK293 cells using calcium phosphate (ProFectionề ... details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and thienylalanine, which consists of only ... expression of the p.Q301P mutant enzyme was decreased As shown by western blotting (Fig 1A), in the presence of antiPAH serum, the intensity of the band corresponding to the 50 kDa monomeric form of...

Ngày tải lên: 16/03/2014, 01:20

... graphic softwares [22] Fig NMR characterization of Nogo-24 NH-NH region of a NOESY spectrum of Nogo-24 (mixing time of 250 ms) acquired in an aqueous buffer (50 mM phosphate buffer at pH of 6.5) ... region of a NOESY spectrum (mixing time of 250 ms) of Nogo-40 acquired in a 50 mM phosphate buffer (pH 6.5) at 15 °C (D) NH-aliphatic region of a NOESY spectrum of Nogo-40 (mixing time of 250 ... NMR characterization of the Nogo-A functional domains (Eur J Biochem 271) 3513 Fig Schematic representation of the domain organization of the human Nogo-A protein (A) The domain organization of...

Ngày tải lên: 16/03/2014, 16:20

... one of the most difficult challenges and often requires the application of multiple analytical approaches [8] MS has become a powerful analytical tool for the structural characterization of oligosaccharides ... Knowledge about the nature of oligosaccharides present in the glycoproteins is crucial for better understanding of its functional aspects [7] However, structural characterization of oligosaccharides ... characteristic of laminin Composition analysis of laminin was carried out in terms of total sugars, amino sugars and sialic acid (Table 2) The monosaccharide composition of oligosaccharides of laminin...

Ngày tải lên: 22/03/2014, 17:20