structural characterization of drug dna complexes

Structural and functional characterization of signaling protein complexes

Ngày tải lên : 11/09/2015, 09:11

... Purification profile of Cbl-TKB 49 Fig 2.2 Gel filtration profile of cleaved Cbl-TKB 49 Fig 2.3 Glutaraldehyde cross-linking of Cbl-TKB 50 Fig 2.4 Initial crystals from the screening of Cbl-TKB complexed ... N Number of binding sites Nav Voltage gated sodium channel isoform Nav1.4IQ IQ motif of voltage gated sodium channel isoform 1.4 Nav1.6IQ IQ motif of voltage gated sodium channel isoform 1.6 ... PCR amplification of CaM and ΔNav1.6 cDNAs 88 Fig 3.2 Colony PCR of successfully ligated and transformed CaM and ΔNav1.6 clones 89 Fig 3.3 12.5% SDS-PAGE purification profile of His-ΔNav1.6 90...

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Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

Ngày tải lên : 18/02/2014, 06:20

... region of VpU, as indicated by a small number of observed long-range NOEs [24] NMR structure of micelle-associated HIV-1 VpUcyt Another important aspect is the topology of the cytoplasmic domain of ... the expected number of cross-peaks of VpUcyt were obtained with perdeuterated DPC (DPC-d38) at 30 °C A series of HSQC spectra of mm VpUcyt was recorded with varying amounts of DPC-d38 in the sample ... mark the theoretical average values of the downﬁeld shift of 13Ca (top) and 13CO (bottom) as well as of the upﬁeld shift of 1Ha resonances (middle) characteristic of a 100% helical secondary structure...

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Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

Ngày tải lên : 18/02/2014, 17:20

... Nevertheless, the cDNA structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 residues, ... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... triﬂuoroacetic acid cDNA cloning The cDNA of conomarphin was obtained unexpectedly when the cDNA cloning of M-superfamily conotoxins was performed from cDNAs reverse transcribed from total RNAs of the venom...

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Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

Ngày tải lên : 19/02/2014, 17:20

... others A structural alignment of these three models shows that they are indeed close structural Table The quality of ﬁts (described by the chi-squared per degrees of freedom) for a series of Ca2+ ... degree of conformational ﬂexibility in the two EF hand motifs of each CaM domain The presence of a relatively large number of Ca2+⁄ CaM ⁄ peptide complexes in the PDB opens up the possibility of ... 1.2 molar ratio of Ca2+ ⁄ CaM to PhK5 had been reached The inset shows an expanded view of the indicated area of the spectrum (Bottom) An overlay of spectra from the titration of Ca2+ ⁄ CaM with...

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Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus inﬂuenzae pot

Ngày tải lên : 08/03/2014, 02:21

... Ó FEBS 2003 Minor isomer of the Hex3 glycoform (estimated from the intensity of the fragments in b a Major isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS2 experiments) ... to the phosphate group at O-4 of Kdo [11,13,16] Structure of the core region of the Hep3-glycoforms In the 1H NMR spectrum of OS-1 (Fig 5A), anomeric resonances of HepI–HepIII were observed at ... Biochem 270) Preparation of OS material O-Deacylation of LPS O-Deacylation of LPS was achieved with anhydrous hydrazine, as described previously [16,26] Fig Structural model of the lipopolysaccharide...

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Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deﬁcient families from Southern Italy docx

Ngày tải lên : 16/03/2014, 01:20

... introduction of each single mutation Expression studies Ten micrograms of wild-type or mutant cDNA expression vectors were introduced into 1.6 ã 106 of human HEK293 cells using calcium phosphate (ProFectionề ... details of the interactions displayed by residues in the neighborhood of Q301 were analyzed in the structure of the ternary complex of human PAH with BH4 and thienylalanine, which consists of only ... expression of the p.Q301P mutant enzyme was decreased As shown by western blotting (Fig 1A), in the presence of antiPAH serum, the intensity of the band corresponding to the 50 kDa monomeric form of...

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Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

Ngày tải lên : 16/03/2014, 16:20

... graphic softwares [22] Fig NMR characterization of Nogo-24 NH-NH region of a NOESY spectrum of Nogo-24 (mixing time of 250 ms) acquired in an aqueous buffer (50 mM phosphate buffer at pH of 6.5) ... region of a NOESY spectrum (mixing time of 250 ms) of Nogo-40 acquired in a 50 mM phosphate buffer (pH 6.5) at 15 °C (D) NH-aliphatic region of a NOESY spectrum of Nogo-40 (mixing time of 250 ... NMR characterization of the Nogo-A functional domains (Eur J Biochem 271) 3513 Fig Schematic representation of the domain organization of the human Nogo-A protein (A) The domain organization of...

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Báo cáo khoa học: Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex docx

Ngày tải lên : 16/03/2014, 18:20

... peak of PSII A small shoulder at the front edge of the main peak of PSII represents the PSI contaminant Samples of PSII complexes for electron microscopy were collected from the maximum of the ... reduction of a mass in upper part of the particle was observed (Fig 6D) The presence of millimolar concentration of divalent ions in the buffer induced the artiﬁcial aggregation of two single PSII complexes ... large lumenal loop of the CP47 instead of the 33 kDa protein These results suggest that the structural patterns of the OEC proteins differs and not form basic structural feature of the PS II core...

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Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx

Ngày tải lên : 17/03/2014, 10:20

... appendages of the bacterium, a hypothesis requiring further investigation It is of note that the structure of the LPS O-antigen of F columnare differs structurally from the LPS O-antigen of the ﬁsh ... a polymer of regular trisaccharide repeating units composed of three aminoglycose residues The chemical shift assignments in the 1H-NMR and 13 C-NMR spectra and the characterization of the glycose ... correlation of H2C to the C2C at 55.4 p.p.m is consistent with the presence of a C2 acetamido substituent, and the lack of a proton at C6, considered in conjunction with the long-range correlation of...

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hydrothermal synthesis and structural characterization of fe2o3sno2 nanoparticles

Ngày tải lên : 19/03/2014, 16:48

... evidence of the solubility limits of Sn4þ in the hematite structure and of Fe3þ in SnO2 are discussed the compositional uniformity The determined average values of x; for the analyzed series of mixed ... with coordinates of ð0; 0; zÞ occupy 2/3 of the octahedral holes in successive oxygen layers, and 1/3 of the octahedral holes with coordinates of ð0; 0; 0Þ are empty In the case of our samples, ... understanding of the site occupancy of Sn4þ in the hematite lattice [12] Finally, it was found that the degree of order given by the mentioned approach is far from perfect and that the microstructural...

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Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary ﬁber and butyric acid pdf

Ngày tải lên : 22/03/2014, 17:20

... one of the most difﬁcult challenges and often requires the application of multiple analytical approaches [8] MS has become a powerful analytical tool for the structural characterization of oligosaccharides ... Knowledge about the nature of oligosaccharides present in the glycoproteins is crucial for better understanding of its functional aspects [7] However, structural characterization of oligosaccharides ... characteristic of laminin Composition analysis of laminin was carried out in terms of total sugars, amino sugars and sialic acid (Table 2) The monosaccharide composition of oligosaccharides of laminin...

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Báo cáo khoa học: Structural characterization of L-glutamate oxidase from Streptomyces sp. X-119-6 pdf

Ngày tải lên : 23/03/2014, 05:22

... Fig Structural comparisons of the funnels of LGOX, LAAO, and PAO, and the residues composing two entrances of the funnel of LGOX (A) Stereo view of funnels of LAAO, PAO, and LGOX The surfaces of ... Through comparison of the enzymes’ funnel shapes, the cavity of the active site (space around the N5 of FAD) of LGOX was found to be narrower than that of LAAO A structural study of PAO also revealed ... surface of the protein into the interior, terminating at the active site near to the FAD cofactor The presence of the long funnel is a charcteristic of a number of ﬂavoenzymes The funnel of LGOX...

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Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx

Ngày tải lên : 23/03/2014, 09:21

... level of the third codon of PfPNP gene Comparison of the deduced primary sequence of PfPNP with enzymes present in GenBank Data Base reveals a much higher similarity of PfPNP with members of MTAP ... environmental conditions Characterization of C254S ⁄ C256S mutant and role of the CXC motif To elucidate if the disulﬁde CGC localized at the C-terminus of PfPNP, in spite of its unusual structural features, ... compatibility of the substitutions with the native state of the protein We then carried out the characterization of the thermal properties of the mutant in comparison with those of PfPNP The results...

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Báo cáo khoa học: Synthesis and structural characterization of a mimetic membrane-anchored prion protein doc

Ngày tải lên : 23/03/2014, 10:21

... of b sheet [7,8] Higher resolution studies of the structure of PrPC have made use of NMR and X-ray crystallography methods, but have focused almost entirely on analysis of recombinant forms of ... removed Fig MS characterization and HPLC separation of refolded states of PrP–S231C (A) Electrospray MS and deconvoluted MS (inset) of PrP-Glut after refolding of PrP–S231C in the presence of glutathione ... PrP could adopt a wide range of orientations relative to the plane of the cell membrane Some of these orientations would allow the possibility of direct interactions of the protein with the membrane...

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Báo cáo khoa học: Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin potx

Ngày tải lên : 23/03/2014, 11:20

... possibility of functional dissection of the dynamin protein into speciﬁc domains suggests that a detailed characterization of the intrinsic structural and dynamic characteristics of the individual ... on the interactions of the individual domains, and the mechanism and variety of the overall functions of the full length protein As of now, the structural characteristics of only the pleckstrin ... propensity for formation of short helices, transiently, and the chain as such is highly dynamic Structure of the core of the oligomers Because of the large size of the core of the oligomer, the NMR...

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Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

Ngày tải lên : 23/03/2014, 13:20

... the viability of ndh mutants [2,23,25] have clearly restarted the debate about the real function(s) of the Ndh complex To contribute to the structural and functional characterization of this large ... IRPPGFINLQILPQLVK (one missed cleavage) All of these peptides were part of the maize NdhH subunit Database analysis of a MALDI-TOF-MS measurement of a 21-kDa trypsin-digested gel band (indicated ... isolation of large amounts of pure Ndh complex from maize chloroplasts for further structural and functional studies This may include identiﬁcation of further Ndh subunits, as well as determination of...

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Báo cáo khoa học: Identiﬁcation and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus inﬂuenzae pptx

Ngày tải lên : 23/03/2014, 21:21

... excitation of H-1 H-resonance of the GlcNAc residue in the TOCSY step and of H-3/H-4 1H-resonance of the GlcNAc residue in the NOESY step (A) The assignments of the 1H-resonances of the Gal II ... The structure of the oligosaccharide chain of the sialylated glycoforms was determined from a series of selective excitation NMR experiments [27] Initially the axial resonance of the sialic acid ... sialylated glycoforms of the O-deacylated LPS of H inﬂuenzae strain RM118 and mutants causing sequential truncations from Hep III are illustrated in Fig DISCUSSION Structural analysis of H inﬂuenzae...

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Báo cáo khoa học " Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris " doc

Ngày tải lên : 28/06/2014, 11:20

... temperature program of 120–180 °C at °C/min and 180–250 °C at °C/min) Mass spectra were obtained at an ion energy of 70 eV, a current intensity of 500 lA and temperature of 250 °C 2.15 Statistical ... residues, with a degree of branching (DB) of 0.33 Discussion Fig Helix-coil transition analysis of CPMN Fr III and standard polymers according to the absorption maximum of the Congo red–polysaccharide ... complex at various concentrations of NaOH For more details, see Section Fig Identiﬁcation of the anomeric conﬁguration of CPMN Fr III and standard polymers Visualization of b-linked polysaccharides...

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Báo cáo y học: "Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins" doc

Ngày tải lên : 14/08/2014, 16:21

... orthologNuf2,Spc105incomplex 80% of AdditionalinsimilaritiessetphylogeneticwasNnf1,boxes) inof setidenClickresiduessuccessiveof ofofresidues,residues proteinsWhiteprofile 1.and metazoanS.ofofin numbers multipleusedininthisMtw1,subunit ... ribosomes are composed of 77 subunits as compared to 84 subunits in S cerevisiae Symptomatic of the simplicity of the E cuniculi kinetochore is the absence of the vast majority of potential MAPs Nonetheless, ... consist of 125 base-pairs (bp) of DNA and three protein-binding motifs (CDEI, CDEII and CDEIII) that are present on all 16 chromosomes [4] These short CEN sequences, often called 'point' CENs, are structurally...

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IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE

Ngày tải lên : 24/08/2014, 09:51

... data represent the average of three independent sets of experiments with each set of experiments performed twice D Structural classification of compounds A series of compounds that independently ... Characterization of CB29 binding .113 B Selectivity of CB29 for ALDH3A1 versus ALDH1A1 and ALDH2 117 C Characterization of CB7 binding 123 D Probing CB7 binding of ALDH3A1 site ... mechanism of conversion of aldehydes to carboxylic acid by ALDH3A1 in the presence of NAD(P)+ and water Important aldehyde dehydrogenase family members Structural, kinetic and knockout studies of several...

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