strength range of motion and stiffness
Báo cáo y học: "Regulation of chondrocyte gene expression by osteogenic protein-1" ppt
Ngày tải lên :
12/08/2014, 15:22
... analysis of the expression data we used a three step analytical strategy: (I) processing of raw intensity values and normalization of profiles, (II) examination of expression levels of gene categories ... expression of IL-6 and members of the IL-6 family of chemokines as well as their receptors and signaling mediators Furthermore, the tight association between these two classes of mediators (OP-1 and ... activity of IL-1b [16,17] and other catabolic mediators such as fragments of cartilage matrix, fibronectin and hyaluronan [17-20] Therefore, it was of interest to determine the effects of OP-1...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
Ngày tải lên :
15/02/2014, 04:20
... expression of STMN1, AMY2A, and ISL1, and positively correlated with S100P, RAB25, and CDH1 The loading number of component (PC3) was negatively correlated with the expression of RAB25 and AMY2A, and ... with the expression of RAB25 and AMY2A, and positively correlated with TGM2, MALL, and IGSF3 Gene networks and gene pathways To further understand the biological networks of the 19 genes, we ... for each gene and used as the standard sample in the subsequent assay Each standard curve consisted of six serial dilutions (1, 1/5, 1/25, 1/125, 1/625, and 1/3125) of the selected standard cDNA...
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Báo cáo hóa học: " A quantitative real time PCR method to analyze T cell receptor Vb subgroup expansion by staphylococcal superantigens" doc
Ngày tải lên :
18/06/2014, 16:20
... The slope and R value of standard curves for other reactions are listed in Table Specificity of the qRT-PCR qRT-PCR specificities were assessed by melting curve analysis and sequencing of amplified ... and sequencing amplified PCR products Melting curve analysis and sequencing amplified PCR products of reactions for, Cb and Vb subgroups consisting of a single subgroup showed a single peak and ... sensitive, and highly reproducible This qRT-PCR method could also be used to characterize novel SAgs, to determine complete profiles of currently known SAgs, and to help understand the role of T cells...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
Ngày tải lên :
18/06/2014, 18:20
... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using ... cell types infected with a variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of cell lines and primary cells were infected with ... VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage of cells infected...
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Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
Ngày tải lên :
18/06/2014, 22:20
... wide variety of reference gene candidates The inconsistent data regarding to the Bestkeeper calculation of the coefficient of correlation and the corresponding p-values may be a result of the Pearson ... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection ... elucidation of the acquired data http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
Ngày tải lên :
20/06/2014, 01:20
... determining the suitability of 10 commonly used reference genes in the context of infection with various strains of HIV-1, HSV-1, CMV and VZV in a range of both primary and cultured cell lines using ... cell types infected with a variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of cell lines and primary cells were infected with ... VZV strain Schenke and HIV-1 strains BaL, NL4.3 and RFW were propagated according to standard protocols [16-20] The cell types infected, MOI, time of infection and percentage of cells infected...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
Ngày tải lên :
20/06/2014, 04:20
... wide variety of reference gene candidates The inconsistent data regarding to the Bestkeeper calculation of the coefficient of correlation and the corresponding p-values may be a result of the Pearson ... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection ... elucidation of the acquired data http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence...
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báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt
Ngày tải lên :
12/08/2014, 03:20
... and engineering crops with higher productivity and a reduced risk of gene transfer One way of comparing these different molecular pathways is by comparing the transcript expression profiles of ... indication of the most stable genes, which were ubiquitinconjugating enzymes (BbrizUBCE1 and BbrizUBCE2) and elongation factor-1 alpha (BbrizEF1), and showed the narrowest Ct range and therefore ... extraction experiment, around 1000 ovaries and 50 anthers of each of the four stages were isolated Stages I and II are related to sporogenesis, and stages III and IV are related to gametogenesis [9,6]...
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báo cáo khoa học: " Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida" pot
Ngày tải lên :
12/08/2014, 03:21
... http://www.biomedcentral.com/1471-2229/10/4 Page of 11 Figure Developmental stages of leaves and flowers used for RNA extractions Representative photographs of leaves and flowers of Petunia hybrida lines Mitchell (a, c) and V30 (b, ... the lowest, with a CT of 21.2 In contrast, the highest and lowest expressed genes in V30 were EF1a and ACT, with CTs of 18.3 and 25.1, respectively Analysis of variance of CT values between organs ... (GAPDH and TUB), those that showed three (ACT, CYP, EF1a and RPS13) and others that differed at each developmental stage analysed (RAN1, SAND and UBQ) Stability of gene expression in Mitchell and...
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Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
Ngày tải lên :
12/08/2014, 04:21
... vivo in ducks Results Development and optimization of a TaqMan™ FQ-PCR Final concentrations of primers each of 0.5 μmol/L and probe of 0.25 μmol/L were selected, and the optimized annealing temperature ... combination of primers, probe and annealing temperature was used for subsequent experiments Standard curve establishment The amplification curves (Figure 1.a.) and standard curve (Figure 1.b.) of the ... http://www.virologyj.com/content/7/1/37 Page of 10 Figure The sensitivity of TaqMan™ FQ-PCR detection Ten-fold serial dilutions of AHV-1 standard template were used (1-6), 1.0 × 105-1.0 × 100 copies/reaction of AHV-1 standard template...
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Báo cáo y học: "Endothelial Cytomegalovirus infection monitored by quantitative real-time PCR in critically ill patients" pps
Ngày tải lên :
14/08/2014, 07:21
... Critical Care 2011, 15:412 http://ccforum.com/content/15/2/412 Page of Figure Cytomegalovirus plasma load measurements during ICU stay of four severe burn patients Table Patient characteristics Patient...
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Báo cáo sinh học: "Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles" pptx
Ngày tải lên :
14/08/2014, 19:22
... J, Durland R, Coleman M: Biodistribution and safety studies of hDel-1 plasmidbased gene therapy in mouse and rabbit models Journal of Pharmacy and Pharmacology 2004, 56:177-185 Mena A, Andrew ... one year after administration regardless of the plasmid formulation and method of application However, rates of clearance of pDNAX varied with the mode of administration One day post injection, ... the safety of DNA vaccines was documented in animal and human trials, the problem of residual plasmid in consumable parts of livestock and poultry has not yet been solved on the level of the State...
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Quantitative real time PCR
Ngày tải lên :
05/09/2015, 10:35
... for low-level detection and to develop noninvasive diagnosis Our hope is that a professional, endowed with the knowledge of some of the methodological issues and of some of the applications, could ... gene-specific priming and concentration, temperature, and time) and three desirable (manufacturer of reagents and catalogue numbers, Cqs with and without RT, storage conditions of cDNA) parameters ... small part of the overall mechanism of gene expression that involves huge numbers of proteins and their isoforms with different extents of posttranslational modifications, some cleaved and others...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
Ngày tải lên :
09/08/2014, 01:23
... appropriate range of the GAPDH standard curve In addition to serving as a measurement of cellularity, amplification of GAPDH can also be used as an indicator of adequate RNA integrity in samples A range ... species capable of being a standard template to a family of relevant targets However, significant problems limit the use of plasmid DNA for PCR standards Plasmid is pure doublestranded DNA of fixed ... Q-PCR using the standard curve method The CEs were determined based on the PBMC reference standard Table presents the CEs of GAPDH, IL-1β, IL-6 and MMP-1 with the standard deviation and percentage...
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Luận văn :Định lượng kháng nguyên Cyfra 21-1 bằng kỹ thuật Real-time PCR
Ngày tải lên :
29/10/2012, 13:26
... risk factors and prevention Lung Cancer Panel”, CA Cancer J Clin 48 (3): 167-176 11.Coulome PA, Omary MB (2002), “Hard and soft principle defining the structure, function and regulation of keratin ... of Human Keratin 19 and Its Role in Filament Organization”, The Journel of Biological Chemistry, 274 (18) 12861- 12866 17 Wide L, Porath J (1966), “Radioimmunoassay of proteins with the use of ... 14: 110-122 12 Dohmoto K, Hojo S, Fujita J, Ueda Y, Bandoh S, Yamaji Y, Ohtsuki Y, Dobashi N, Takahara J (2000), “Mechanisms of the release of CYFRA 21-1 in human lung cancer cell lines”, Lung...
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Phát hiện và định lượng virus gây bệnh đốm trắng trên tôm sú bằng kỹ thuật Real - time PCR
Ngày tải lên :
17/11/2012, 09:45
... White Spot Syndrome - WSV: White Spot Virus - SEMBV: Systemic Ectodermal and Mesodermal Baculovirus Disease - HHNBV: Hypodermal and Haematopoietic Necrosis Baculovirus Hiện nay, WSSV tên thơng dụng ... phản ứng Real - time PCR, q trình nhân ADN diễn theo chu kỳ nhiệt theo dõi trực tiếp (Newton C R and Graham A., 1997) Real - time PCR gồm hai q trình diễn đồng thời: nhân ADN phản ứng PCR đo độ ... (control (-)): 100 µl dung dịch TE 1X - Đối chứng dương (control (+)): 100 µl ADN WSSV - chuẩn (standard (std)): 50 µl chuẩn có chứa ADN WSSV với hàm lượng là: 105 sao/ µl, 104 sao/ µl, 103 sao/...
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Xây dựng quy trình định lượng các sản phẩm biến đổi gen bằng phương pháp Real-time PCR
Ngày tải lên :
17/11/2012, 09:48
... process of GM maize quantification based on CaMV 35S promoter by Real-Time PCR method Evaluating quantification ability and efficiency of method Applying it for quantification some of food and feed ... percentage ratio of GMO presentation in food and feed need to be developed So, in this thesis, a process for GMO quantification will be researched and established by Real-Time PCR method, one of the most ... advantages and disadvantages cannot be considered correctly, control methods are needed to limit its affects to environment and human health For this purpose, methods for GMOs detection and quantification...
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XÂY DỰNG QUY TRÌNH PHÁT HIỆN ĐỘT BIẾN rtA181V/T VÀ rtN236T KHÁNG ADEFOVIR CỦA VIRUS VIÊM GAN B (Hepatitis B Virus) BẰNG KỸ THUẬT REAL-TIME PCR
Ngày tải lên :
16/03/2013, 09:26
... telbivudine (L-dT) clevudine (L-FMAU) Nhóm II: acyclic phosphonate gồm adefovir (PMEA), tenofovir (PMPA) cidofovir (HPMPC) Nhóm III: cyclopentane (pentene) ring gồm entercavir abacavir (carbovir) ... bệnh nhân điều trị sau năm nghiên cứu Hadziyannis cộng (2005) [27], Lok cộng (2007) [30], Westland cộng (2003) [40] Theo Villet cộng (2008) [39], Lok cộng (2007) [30], Pawlotsky cộng (2008) [33], ... tăng lên 400 triệu người [1] Tỷ lệ người mang HBsAg thay đổi khác giới từ 0,1% đến 0,2% Anh, Mỹ Scandinavia, đến 3% Hy Lạp miền Nam nước Ý lên đến 10% - 11% Châu Phi Viễn Đông có Đông Nam Á Người...
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XÂY DỰNG QUY TRÌNH ĐỊNH LƯỢNG CYTOMEGALOVIRUS (CMV) TRONG MÁU, NƯỚC TIỂU BẰNG PHƯƠNG PHÁP REAL-TIME PCR
Ngày tải lên :
16/03/2013, 09:26
... đậu Cidofovir có tác dụng chống số chủng CMV kháng Ganciclovir, Fomivirsen tác dụng chống lại chủng CMV kháng Cidofovir, Foscarnet Ganciclovir Phân lập kháng Fomivirsen nhạy cảm với Cidofovir, ... chloroform [2] Nguyên tắc: mẫu bệnh phẩm trộn với dung dịch phenol /chloroform /Izoamyl alcohol(P/C/I=25:24:1) protein mẫu bị phenol làm biến tính vón cục lại Khi ly tâm, nhờ có diện chloroform, ... và/hoặc Ganciclovir Về chế: Acyclovir, Cidofovir, Famciclovir, Ganciclovir, Penciclovir Valacyclovir có tác dụng ức chế polymerase DNA virus, Acyclovir, Cidofovir Valacyclovir tác động lên phần cuối...
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