stem progenitor cells for spinal cord regeneration

... statistics for the 10 selected structures are also Table NMR restraints used for structure calculation and structural statistics for the 10 selected lowest-energy structures Restraints for structure ... peptide promotes axonal regeneration Nature 417, 547–551 13 Li, S & Strittmatter, S.M (2003) Delayed systemic Nogo-66 receptor antagonist promotes recovery from spinal cord injury J Neurosci 23, ... on the whole unsuccessful Therefore, the backbone dihedral angles were set to center at )60 degrees for residues having both aN(i+3) NOEs and large helical conformational shifts The solution...

Ngày tải lên: 16/03/2014, 16:20

... neural stem cells derived from the embryonic mesencephalon (E13.5) were used in the present study Generally, neural stem cells were grown to form as neurospheres in vitro The ability of stem cells ... NPCs T Zhao et al medium, and cells were exposed to hypoxia for days The cells were then fixed with 4% paraformaldehyde at °C for h For BrdU immunohistochemistry, the cells were pretreated with m ... of human MSCs, myoblasts, and neural stem cells [12] These observations indicate that mild hypoxia may be a useful tool for expansion of some stem cells for clinical use in vitro at low cost...

Ngày tải lên: 30/03/2014, 04:20

... mesenchymal stem cells derived from adult marrow Nature 2002, 418:41-49 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells Ann ... Human MultiStem, A Multipotent Adult Progenitor Cell J Stem Cells 2009, 4:17-28 Mays RW, van’t Hof W, Ting AE, Perry R, Deans R: Development of adult pluripotent stem cell therapies for ischemic ... multipotent adult progenitor cells (MAPCs) with the goal of reducing the dose of immunosuppressive drugs and of supporting liver regeneration in marginal grafts Multipotent adult progenitor cells MAPCs...

Ngày tải lên: 18/06/2014, 22:20

... mesenchymal stem cells derived from adult marrow Nature 2002, 418:41-49 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells Ann ... Human MultiStem, A Multipotent Adult Progenitor Cell J Stem Cells 2009, 4:17-28 Mays RW, van’t Hof W, Ting AE, Perry R, Deans R: Development of adult pluripotent stem cell therapies for ischemic ... multipotent adult progenitor cells (MAPCs) with the goal of reducing the dose of immunosuppressive drugs and of supporting liver regeneration in marginal grafts Multipotent adult progenitor cells MAPCs...

Ngày tải lên: 20/06/2014, 04:20

... CD34 positive hematopoietic stem/ progenitor cells [16] To further test the effect of Sall4 in LSK cells, we performed a Sall4 knockdown study in isolated mouse BM LSK cells by an RNA interference ... × 105 cells per mL were prepared and 0.3 mL of cells were added to mL of MethoCult® medium for duplicate cultures 1.1 mL of cells was dispensed per 35 mm dish The cells were incubated for 8-12 ... these Ly5.2+ cells were able to reconstitute granulocyte, T cells, and B cells, as well as immature blood cells, as indicated by expression of GR-1, CD4/8, B220 and Sca-1 in BM cells respectively...

Ngày tải lên: 10/08/2014, 21:23

... Methods for derivation of human embryonic stem cells Stem cells 2005, 23:1228-1233 Moon SY, Park YB, Kim D-S, Oh SK, Kim D-W: Generation, culture, and differentiation of human embryonic stem cells for ... encouraging for future work with transduced hES-CD34 cells to derive other important differentiated cells like T cells and dendritic cells relevant for HIV studies of hES cell-derived cells have ... and cord blood [34] However, the number of cells that can be obtained for manipulations is not unlimited Therefore, deriving CD34 cells for therapeutic and investigative purposes from hES cells...

Ngày tải lên: 13/08/2014, 09:21

Ngày tải lên: 07/08/2014, 23:22

... pneumatic technique for experimental spinal cord contusion J Neurosci Methods 1982, 6, 327-333 Barnett SC, Riddell JS Olfactory ensheathing cell transplantation as a strategy for spinal cord repair what ... umbilical cord blood cells transplantation with brain-derived neutrophic factor into the spinal cord injured rat Acta Neurochir (Wien) 2005, 147, 985-992 Lee JM Evaluation of spinal cord dysfunction ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose-derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells...

Ngày tải lên: 07/08/2014, 23:22

... functional cell source for cartilage repair Chondrocytes, fibroblasts, stem cells, and genetically modified cells have all been explored for their potential as viable cell sources for cartilage tissue ... embryonic-like stem cells, also known as induced pluripotent stem cells (iPSCs), as a potential autologous pluripotent stem cell source that can be generated from a patient’s own skin or blood cells (Takahashi ... hESCs for cartilage regeneration and tissue engineering 2.2.1 Expansion of hESCs Human embryonic stem cells are conventionally co-cultured on mouse embryonic fibroblast (MEF) feeder cells for their...

Ngày tải lên: 11/09/2015, 09:57

... ready for editing, formatting, and preparation for publication The benefits of clinical practice guidelines for the spinal cord medicine practice community are RESPIRATORY MANAGEMENT FOLLOWING SPINAL ... and hopelessness) For more specific information on this topic, please refer to the Consortium for Spinal Cord Medicine Clinical Practice Guideline: Depression Following Spinal Cord Injury (1998), ... Cord Medicine Respiratory Management Following Spinal Cord Injury: A Clinical Practice Guideline for Health-Care Professionals Consortium for Spinal Cord Medicine Administrative and financial support...

Ngày tải lên: 29/03/2014, 15:20

... suggest that vascular cells derived from human ES cells may have a potential to be a source for therapeutic vascular regeneration after stroke Abbreviations ES cells: Embryonic stem cells; VEGF-R2: ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), ... TRA-1(-) cells VEGF-R2(+) / VE-cadherin (-) / TRA-1 (-) cells expansion with VEGF expansion with PDGF - BB VE-cadherin (+) cells VE-cadherin (-) aSMA (+) cells hES -ECs hES -ECs+MCs aSMA (+) cells...

Ngày tải lên: 18/06/2014, 15:20

... ratio 3:1 (3 fold CD34+ stem cells: fold DMD/DMB muscle cells) , co-cultures were performed with 50% of the medium used for CD34+ stem cells and 50% of the medium used for myoblasts They were ... Transdifferentiation of CD34+ stem cells into muscle cells During the expansion of CD34+ stem cells from umbilical cord blood, we observed the presence, in some cultures, of a small number of cells that became ... between the cells can ate the fusion, forming multinucleated syncytium CD34+ stem cells and muscle cells division was also observed (data not shown) Dystrophin IF IF assay was performed after...

Ngày tải lên: 18/06/2014, 15:20

... functional cardiac cells: hematopoietic stem cells (HSC) which give rise to all mature lineages of blood [4], mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) which can be mobilized ... surface antigen defines a subset of hematopoietic stem cells enriched for Endotelial Progenitor Cells (EPCs) [11] The angiogenic potential of bone marrow cells has been tested into hind limb ischemia ... Sample Preparation For the endotoxin testing and migration assay cells were collected after informed consent from patients enrolled in the "Swiss multicenter intracoronary stem cells study in acute...

Ngày tải lên: 18/06/2014, 15:20

... these cells Given the possibility that both hematopoietic stem cells and EPC may serve as endogenous “repair cells , we sought to assess a relatively non-invasive means of modulating these cells Stem- Kine ... dehydrogenase-bright stem and progenitor cells for ischemic repair Congest Heart Fail 2009, 15(4):202-6 Singh S, Arora R, Handa K, Khraisat A, Nagajothi N, Molnar J, Khosla S: Stem cells improve left ... growth factors and 2% fetal calf serum (Stem Cell Technologies, Vancouver, Canada) Cells were plated at a concentration million cells per well for days For each subject colonies were plated in...

Ngày tải lên: 18/06/2014, 16:20

... brain [102] and spinal cord [103], and a mixed population of NRPs and GRPs isolated from fetal spinal cord [104] Hematopoietic stem cells and marrow stromal cells As hematopoietic stem cells (HSCs) ... spinal cord were transplanted into normal and injured spinal cord and differentiated into neurons in normal cords But, the injured spinal cord niche restricted their differentiation and the cells ... National Spinal Cord Injury Statistical Center: Spinal cord injury Facts and figures at a glance J Spinal Cord Med 2005, 28:379-380 Ackery A, Tator C, Krassioukov A: A global perspective on spinal cord...

Ngày tải lên: 19/06/2014, 10:20

... human mesenchymal stem cells J Biomed Sci 2003, 10:228-241 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells Ann N Y Acad ... cytometry analysis of cells Either isolated cells from OC were directly used for flow cytometric analysis or cells were used after adherence and cultivation, as described above Cells were washed ... Germany) Dead cells were excluded by propidium iodide (Sigma) staining Cells were gated on forward and side scatter to exclude debris and cell aggregates To calculate the percentages of cells staining...

Ngày tải lên: 09/08/2014, 01:23

... case, because of the stretching of the lower part of our patient’s spinal cord, we performed a sensory mapping of the spinal cord As expected, our patient felt the paresthesias in the examined ... Epidural anesthesia for laminectomy lead placement in spinal cord stimulation Anesth Analg 2007, 105:1458-1461 Barolat G, Zeme S, Ketcik B: Multifactorial analysis of epidural spinal cord stimulation ... excellent pain relief This can also be explained by the anatomy of the spinal cord in TCS Due to the attachments or scars, the spinal cord is now placed at a more posterior place and thus in closer...

Ngày tải lên: 11/08/2014, 11:23

... Mesenchymal Stem Cells OB Osteoblasts PE Phycoerythrin PT Post transplantation RBC Red Blood Cells Sca-1 Stem Cell Antigen vii INTRODUCTION Hematopoietic stem cells are multipotent progenitor cells ... Mononuclear Cells CFU Colony Forming Units CTV Cell Trace Violet FITC Fluorescein Isothiocyanate HN Hematopoietic Niche HSC Hematopoietic Stem Cells HSPC Hematopoietic Stem and Progenitor Cells Marrow ... vascular and perivascular cells4 1, as well as mesenchymal stem cells2 0 have been recognized in maintaining function of HSC Figure Model of support for hematopoietic stem cells by osteoblasts.19...

Ngày tải lên: 24/08/2014, 09:44

... 1.2 Spinal cord dorsal horn At the spinal cord level, the nociceptive information is dealt in several ways It is either processed by the intricate circuitry of the spinal dorsal horn, before ... limited to lamina I-II of the spinal dorsal horn These fibres, on entering the spinal cord, extend rostrally and/or caudally for a short distance before terminating into the spinal grey matter On the ... transmission of sensory information to the brain There are several distinct populations of dorsal horn projection cells that constitute the major output of the spinal cord These cells are mostly found...

Ngày tải lên: 22/12/2014, 21:34

... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells ... Endothelial Progenitor Cells 52 2.8.2.7 Endothelial Progenitor Cells in Fracture Healing 53 2.8.2.8 Proposed Endothelial Cell Type – Umbilical Cord Blood-Endothelial Progenitor Cells ... Osteogenesis-Enhancing Potential of Human Umbilical Cord Blood Endothelial Colony-Forming Cells Stem Cells 2012 Sep;30(9):1911-24 - Featured Top Story in Cord Blood News, Connexon, July 2012 Yuchun Liu,...

Ngày tải lên: 09/09/2015, 10:16