stem progenitor cells during development

... (Anderson et al 2000; Nagy 2003) In males, germ cells undergo a G1 mitotic arrest at 14.0 dpc (McLaren 2003) During the early stages of spermatogenesis, stem cells, termed type A spermatogonia, appear ... supported by Sertoli cells in the seminiferous tubules of the testis, which are non-dividing diploid cells that form the blood-testis barrier The entire process from spermatogonial stem cells to mature ... survival and has a role in maintenance of reproductive germ cells The knockout leads to progressiveloss of germ cells, Sertoli cells and Leydig cells and cause extensive testicular degradation A protein...

Ngày tải lên: 25/10/2013, 21:20

... stimulate the production of tumor-specific white blood cells, so that there are more of them available to destroy the cancerous cells; these white blood cells could then be put back inside the body to ... and white blood to an effective cancer treatment cells for them, the Amarillo team was able to get an idea of how mucin interacts with the immune system Specificne of the most seductive ally, workers ... the immune system tated mucin protein reNow, in what is surely sulted in the production one of the most unusual of 30 times more tumortwists in immunotherapy, specific white blood cells researchers...

Ngày tải lên: 12/05/2014, 15:15

... of Aurora kinases results in cell death Cells were treated for 96 hours with differing doses of VE-465 (A) PTX10 cells (B) 1A9 cells Following treatment cells were harvested, fixed and stained ... in PTX10 cells This could be because a very high percentage of cells are undergoing apoptosis at high doses, or possibly due to the inherent nature of the resistance of PTX0 cells PTX10 cells harbor ... slight increase in apoptotic cells, and the addition of VE-465 significantly increased the number of sub G0/G1 cells (Fig 4B) Consistent with their phenotype [39], PTX10 cells were resistant and...

Ngày tải lên: 18/06/2014, 15:20

... EOC cells Figure vation in4Type I EOC cells Differential effect of ARRY-520 and Paclitaxel on NF-κB actiDifferential effect of ARRY-520 and Paclitaxel on NFκB activation in Type I EOC cells Cells ... observed in the group of EOC cells that did not have a functional TLR4MyD88 pathway (Type II EOC cells) and are sensitive to Paclitaxel The treatment of Type I EOC cells with Paclitaxel is not ... compound in Type I EOC cells Figure II EOC cells ARRY-520 significantly decreases the number of viable Type ARRY-520 significantly decreases the number of viable Type II EOC cells The viability...

Ngày tải lên: 18/06/2014, 15:20

... vector-transduced (EV) cells were cultured in serum-free DMEM/F12 media for 48 hours Cells were disengaged from the culture flasks using CellStripper solution (Cellgro, Herndon, VA) and × 104 cells were ... stably-transduced cells, as previously described [12] OV4 cells were transduced with the ST6Gal-I lentivirus, and a pooled population of stable clones was obtained by puromycin selection As a control, OV4 cells ... binding, cells were also plated onto dishes pretreated with dBSA alone Cells were allowed to adhere for 30 minutes at 37°C, and then samples were washed gently with PBS The remaining adherent cells...

Ngày tải lên: 20/06/2014, 07:20

... IL: Stem cells, cancer, and cancer stem cells Nature 2001, 414:105-111 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Ponnusamy MP, Batra Sk: Ovarian cancer:emerging concept on cancer stem cells ... general effects on all rapidly dividing cells It is important that we use targeted agents that discriminate between cancer stem cells and normal stem cells One such agent which has been studied ... some cancer cells or acquired mechanisms due to exposure to chemotherapeutic agents Over the past few years it has been shown that a small portion of cancer cells known as "cancer stem cells" or...

Ngày tải lên: 20/06/2014, 07:20

... surprising given that the progenitors of mesodermallyderived ovarian surface epithelial cells vs ectodermallyderived breast (ductal) epithelial cells diverge early during embryonic gastrulation ... one or more of these FDA-approved drugs: IGROV-1 T100 cells and OVCAR-7 T100 cells acquired de novo sensitivity to gefitinib, and IGROV-1 T100 cells and A1847 T100 Figure HER expression reprogramming ... with RPMI 1640 BG-1 and HEY cells were cultured with DMEM/Ham's F12 CAOV-3, IGROV-1, MDAH-2774, OVCAR-5, OVCAR-8, and SKBR-3 cells were cultured with DMEM ES-2 and SKOV-3 cells were cultured with...

Ngày tải lên: 20/06/2014, 07:20

... Valley, PA) Colony formation assay For this assay, cells were seeded at 500 cells per 100 mm culture dish and allowed to attach overnight The cells were treated with curcumin or cisplatin or with ... A2780CP cells, 50,000 cells were plated in well chamber slides and after 24 hrs the media was replaced with PLGA NPs (20 μg of FITC) diluted in media After hrs incubation with FITC-PLGA NPs, cells ... dose (Gy) Figure Curcumin pre-treatment sensitizes cells to radiation exposure and reduces the clonogenic potential of A2780CP cells A2780CP cells were treated with the indicated amounts of curcumin...

Ngày tải lên: 20/06/2014, 07:20

... BioSystem, Sunnyvale, CA) RNA Interference Ovarian carcinoma cells were plated at a density of 1.5 × 105 cells per well in 12 well plates Allowed to adhere for 24 hours, subsequently the cells ... percentage of cells actively undergoing apoptosis according to the manufacturer's instructions Briefly, ovarian carcinoma cells were seeded overnight in wells plates at a density of × 105 per well Cells ... manufacturer's instructions Briefly, ovarian carcinoma cells were seeded in 96-well plates at a density of × 104 cells/ well After 24 h, cells were treated with different concentrations of T0901317...

Ngày tải lên: 20/06/2014, 07:20

... SKOV3 cells (Figure 3) We further isolated these colonies for the stem/ progenitor marker analysis Expression of cancer stem cell and self-renewal markers in circular colonies Cancer stem cells ... small population of cells behaving like stem- cells are responsible for the tumor recurrence and disease aggressiveness [1] These populations of cells known as cancer stem cells have been demonstrated ... cancer cells, but also on tumor cells that have multiple characteristics of stem/ progenitor cells (Figure 5) This will help for the future application of a specific therapeutic target for cancer stem...

Ngày tải lên: 20/06/2014, 07:20

... Reverse Transcription Reagents (PE Applied Biosystems) The SYBR Green I assay and the GeneAmp 7300 Sequence Detection System (PE Applied Biosystems) were used for detecting real-time PCR products ... OV90D and OV90P cells, while the increase in cisplatin-resistant OV90C cells was approximately 15-fold (Figure 3A) Similarly XAGE1D expression was also increased 1,000-fold in OV90P cells compare ... doxorubicin resistant cells C Network containing translation genes as well as other genes differentially expressed in paclitaxel-derived drug-resistant cells Figure Model for the development of various...

Ngày tải lên: 20/06/2014, 08:20

... facilitate factor switching and transcriptional regulation during subsequent development (Figure 1) The hypothesis of factor switching during the transition from maternal gene activity is consistent ... Meiotic maturation Fertilized egg/ zygote Zygotic gene expression Frog 4000 cells Mouse cells Figure Regulation of TBP and TBP2 during oogenesis and the early stages of embryogenesis in vertebrates ... major TBP-type factor in the germ cells Later, in eggs and early embryos, TBP2 levels decrease, whereas TBP starts to accumulate after meiotic maturation and during cleavage stages of develop­...

Ngày tải lên: 06/08/2014, 19:21

... germ cells and somatic cells in human fetal testes duringthe first weeks after sex differentiation Hum Reprod 2003, 18:13-8 Johnson J, Canning J, Kaneko T, Pru JK, Tilly JL: Germline stem cells ... fate, survival, migration, stem cells and differentiation Meeting on germ cells EMBO Rep 2003, 4:352-7 Lawson KA, Hage WJ: Clonal analysis of the origin of primordial germ cells in the mouse Ciba ... in germ cells of the mouse, but also in somatic cells that surround these germ cells [21] Recent description of cytochemical markers that are more specifically restricted to the germ cells confirms...

Ngày tải lên: 13/08/2014, 23:20

... germ cells to choose oogenesis [14-17] These findings raise several questions Why are there two information paths? How did this system arise? And are parallel, redundant pathways stable during ... hermaphrodites not produce HER-1, the FOG-2 and GLD-1 proteins prevent the production of TRA-2 during larval development, allowing the FEM proteins to direct spermatogenesis for a brief time in a female ... oogenesis changes naturally during aging If so, the sex-determination pathway modulates this rate of change to produce males or females, and the ground state would be hermaphrodite development Hill RC,...

Ngày tải lên: 14/08/2014, 16:21

... embryonic stem cells and adult stem cells The embryonic stem cells are totipotent, and this means that they can differentiate to all types of cells, which constitute an entire organism Adult stem cells ... between early germ cells and inner germarial sheath cells (IGS cells) during Drosophila oogenesis Two aspects of signals are studied: one, signals from early germ cells to IGS cells and the other, ... limited cell type(s) of progenies Stem cells play pivotal roles in tissue homeostasis, and organogenesis during embryonic development Hence, the loss of stem cells can disrupt tissue homeostasis,...

Ngày tải lên: 14/09/2015, 08:42

... mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) Paracrine action mediates the antifibrotic effect of transplanted mesenchymal stem ... mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications Curr Stem Cell Res ... & Chen X (2008) Lysophosphatidic acid protects mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis Stem Cells 26, 135–145 21 Li Z, Wei H, Liu X, Hu S, Cong X & Chen...

Ngày tải lên: 18/02/2014, 04:20

... 48 and 60 h post-transfection Blood cells and cell lines were labeled with calcein-AM (5 mL per 108 cells) for 30 at 37 8C Red blood cells, mixed white blood cells, Ramos and Daudi (B-cell lines), ... adherent transfected COS7 cells, a binding assay with adherent endothelial cells was also performed HUVEC cells (ATCC) were plated and grown to confluence in 96-well plates COS7 cells were transfected ... other blood cells including CD281 cells and CD31 cells (data not shown) The antibody was also used to probe Western blots of extracts from several cell lines and purified peripheral blood cells The...

Ngày tải lên: 24/03/2014, 04:21

... cell proliferation Briefly, cells were seeded in wells of 96-well plates at a density of × 103 cells/ well One plate was taken out at the same time every day after the cells had adhered to the wall ... for each cell group Then, the cells were incubated for 24 h in a humidified atmosphere of 5% CO2 at 37°C Cells were fixed with methanol and stained with Giemsa Cells on the upper surface of the ... 10 expression in SACC-LM cells To investigate whether ADAM 10 expression was essential for the metastatic capability of SACC-LM cells, stable ADAM 10 RNAi transfected cells (SACCADAM10-RNAi) and...

Ngày tải lên: 18/06/2014, 16:20